Claude Andary

• Professor of Phytochemistry
• Université de Montpellier

Le genre Aquilaria Lam. (Thymelaeaceae) comprend 21 espèces d’arbres (The Plant List, 2013) et se trouve principalement en Asie du Sud-Est. Lorsque l’arbre est infecté (champignons, bactéries), son bois devient brunâtre ou noirâtre (appelé bois d’agar) en raison de la sécrétion d’une oléorésine en réaction à l’infection. La résine est très parfumée...

Aquilaria is a tree species belonging to the Thymeleaceae family. When Aquilaria sp. is injured, it can produce agarwood. Agarwood is characterized by a darker wood colour than the healthy one and by a strong perfume that is much esteemed by perfumers and some oriental religious communities. The production of agarwood is presumed to depend on envir...

The challenge is to contribute to the sustainable production of high quality Agarwood in French Guiana through the understanding of microbial factors involved in Agarwood formation.

Aquilaria is a tree species belonging to the Thymeleaceae family. When Aquilaria sp. is injured, it produce agarwood. Agarwood is characterized by a darker wood colour than the healthy one and by a strong perfume that is much esteemed by perfumers and some oriental religious communities. The production of agarwood is presumed to depend of environme...

The structure of echinacoside, isolated from Echinacea angustifolia D.C. (Asteraceae) roots has been determided by ¹ H-NMR-and ¹³ C-NMR-spectra to be β-(3′,4′-dihydroxyphenyl)-ethyl-O-α-ʟ-rhamnopyranosyl (1 → 3)-O-β-ᴅ-[β-ᴅ-glucopyranosyl (1 → 6)]-(4-O-caffeoyl)-glucopyranoside (cf. Fig. 1).

The caffeoyl derivatives of Stachys leaves (Labiateae) were identified as chlorogenic acid, verbascoside and stachyoside. The latter compound, reported here for the fist time, was shown to be a verbascoside derivative containing an extra glucose residue attached to C-2, C-3 or C-4 of rhamnose. Cotyledonary leaves of Stachys accumulate high levels (...

This report describes a study on the effects of pectinase and hydrogen peroxide (H2O2) on previously identified phenolic compounds in the leaves of Thunbergia alata. The elicitors were found to produce lesions resulting in the loss of chlorophyll and death of the cells surrounding the point of elicitation. At the optimal concentrations, hydrogen pe...

Some phenolic compounds were studied histochemically before and after infection during two kinds of interactions: Helianthus resinosus/Sclerotinia sclerotiorum and Vitis spp./Plasmopara viticola. H. resinosus is a wild sunflower species chosen for its high tolerance to S. sclerotiorum. For grapevine, three species of different susceptibilities were...

In the poplar/mistletoe association, the resistance phenomenon was investigated using histochemistry, histofluorescence and analytical methods. Among the six poplar cultivars tested, the cv. “Fritzi Pauley” was sensitive, the cvs “Blanc de Garonne”, “Vereecken” and “Italica” were totally resistant whereas the cvs “1214” and “Bergerac” were intermed...

The present invention relates to the use of chicoric acid and/or at least one of these metabolites selected among mono-caffeoyl-tartaric acid and/or m-hydroxyphenylpropionic acid, and/or 4-vinylcatechol and/or 4-ethylcatechol for manufacturing a composition intended for prevention or treatment in an insulin-resistant patient or hypoinsulinemia and/...

Several species of the genus Teucrium section polium from the mediterranean region are difficult to identify because of their wide morphological variability. The relationship between the morphological variations and the distribution of caffeic acid esters was studied. The results show that the taxonomic value of these markers is different depending...

Chemical BasisNatural Sources for AnthocyaninsApplicationsExamples of Commercial Products and ProcessingReferences

In the context of new alternative crop development in Europe, flavonoids were investigated in saw-wort, Serratula tinctoria L., a perennial Asteraceae which was used as a yellow dye until the 19th century. The phytochemical study described in this report indicates that leaves rather than stems should be used, and harvested at the end of the plant g...

A beta-glucosidase enzyme activity was enriched from skins of ripe grape berry by cell wall fractionation, hydrophobic interaction and cation-exchange chromatographies. This enriched enzyme extract contained several beta-glycosidase activities hydrolyzing a wide range of synthetic and natural monoglycosides and diglycosides, as well as a beta-fruct...

Flavonoids extracted from marigold flowers were investigated for their dyeing potential. Patulitrin (1) and patuletin (2) were isolated and their structures established using NMR and HPLC-MS. These compounds were identified as the main flavonoids present in the dyeing bath. Following the dyeing process, it was demonstrated that aglycone 2 bound mor...

Aqueous extracts of plant by—products (carrot, onion, black carrot, sage, spinach and thyme) were investigated for dyeing capacity on fibres and for both colorant and antioxidant potential using colorimetric and chromatographic tools, and FTC assay, respectively. Regarding fibres, classical correlations between measured colours and phytochemical pa...

A new, easy, rapid and relatively inexpensive method using microscopy has been developed for the detection of herbicide effects in leaves of grass weed species displaying no visual signs of damage. The method has potential to be used as a tool to indicate future death of grass species due to herbicide exposure by observing phytochemical effects, i....

The paper reports an investigation of the colouring properties of 43 dyeing plants chosen for their widespread use in previous centuries. Colorimetric analysis showed that the principal colours on different fabric supports were yellow and mostly unsaturated. The nature of the support fibres investigated, protein- or cellulose-based, was shown to pl...

Oral Comunication

Arenarioside (1) and pheliposide (2), caffeic acid glycoside esters, have been isolated from Orobanche arenaria by droplet countercurrent chromatography. The structures have been realized by high resolution 1H- and 13C-nmr studies. Compound 1 is β-(3′,4′-dihydroxyphenyl)-ethyl-O-α-L-rhamnopyranosyl (1→3)-β-D[β-D-xylopyranosyl (1→6)]-(4-0-caffeoyl)-...

Globe of Science, 3, 62-66 (2004). - Aqueous and methanol extracts obtained from fruiting bodies of lignicolous basidiomycete mushrooms Pholiota squarrosa, Pholiota gummosa, Kühneromyces mutabilis, Pleurotus ostreatus and Armillaria mellea (Basidiomycetes) have been studied for content of free sugars, free amino acids, phenolic acids, fungal toxins...

Cocoa breeders and growers continue to face the problem of high heterogeneity between individuals derived from one progeny. Vegetative propagation by somatic embryogenesis could be a way to increase genetic gains in the field. Somatic embryogenesis in cocoa is difficult and this species is considered as recalcitrant. This study was conducted to inv...

Contents of phenolic compounds in leaf extracts often serve as a measure of plant anti-herbivore defence. This method suffers from the multifunctionality of phenolics and from problems with their colorimetric quantification. Here we present further evidence for the pertinence of these problems. Contents of condensed tannins (CCT) were spectrophotom...

Since its original formulation by Janzen in 1966, the hypothesis that obligate ant‐plants (myrmecophytes) defended effectively against herbivores by resident mutualistic ants have reduced their direct, chemical defence has been widely adopted. We tested this hypothesis by quantifying three classes of phenolic compounds (hydrolysable tannins, flavon...

We describe the identification of three phenolic compounds (caffeoylmalic, feruloylmalic and p-coumaroylmalic acids) in the leaves of Thunbergia alata. Caffeoylmalic and feruloylmalic acids represent the majority of all the derivatives of hydroxycinnamic acid in this plant. Elicitation with 5 mM of salicylic acid (SA) after wounding produces an int...

Résumé: La composition en substances volatiles des feuilles, tiges et racines de Peganum harmala a été étudiée sur matériel frais et sec. Trente et un composés ont été identifiés notamment l’acide propylique, le 2,3-dihydrobenzofurane, le 3-octanone et l’isovanilline. Les feuilles sont plus riches en substances volatiles que les racines et les tige...

The effects of various degrees of water deficit on the synthesis and concentration of skin phenolic compounds (flavan-3-ols, anthocyanins, and flavonols) on growing Shiraz berries were studied. Different periods and levels of water stress were applied to a model system of cultured vines in containers. Treatments consisted of two levels of early wat...

Poster : Tahrouch S., Cosson L., Rapior S., Idrissi Hassani L.M., Rouhi R., Gargadennec A. et Andary C. Identification des flavonoïdes de Argania spinosa. Xèmes Journées Scientifiques Nationales STOLON (Marseille, France, 9-10/09/1999), P20. In : Acta Botanica Gallica, 149 (1), 113-114 (2002)._____ Argania spinosa (L.) Skeels (Sapotaceae) est une e...

Although observations suggest pairwise coevolution in specific ant-plant symbioses, coevolutionary processes have rarely been demonstrated. We report on, what is to the authors' knowledge, the strongest evidence yet for reciprocal adaptation of morphological characters in a species-specific ant-plant mutualism. The plant character is the prostoma,...

The use is reported of Mirande's reagent in epifluorescence microscopy which permits a clear distinction between cellulosic and lignified tissues. Homogeneous Prespermatophytae and gymnosperm xylem appeared entirely green with Mirande's reagent under ultraviolet excitation, whereas heteroxyled angiosperm wood showed a mixed pink and blue–green colo...

Black walnut (Juglans nigra L.) heartwood is characterised by the appearance of a dark-brown colour in the inner part of the trunk. At the cellular level, phenolic compounds and peroxidases (PODs, EC 1.11.1.7) are most likely involved in the generation of this colour. Ortho-diphenols are revealed by Arnow’s reagent and condensed tannins by vanillin...

Three mushroom species—Flammulina velutipes, Paxillus involutus, and Tricholoma pardinum (Basidiomycota)—were investigated for pharmacological activities on mice after intraperitoneal and per os administration of fruiting body extract. In particular, analgesic and spasmolytic activities of the mushroom extracts as well as their effects on the centr...

The photostabilities of luteolin, in solution, in the presence of aluminium ions, and deposited on a cellulosic substrate have been determined and compared with those of quercetin and other 5-hydroxyflavonols and their 3-O-glycosides.In aqueous methanol solution, luteolin and flavonol 3-glycosides exhibited no degradation over periods of up to 15 h...

Laetiporus sulphureus was investigated for volatile compounds by GC/MS. Twenty-six components were identified with major constituents being (Z)-3-methylcinnamaldehyde (27.5%), 2-phenylethanol (6.4%), benzaldehyde (4.0%) and N-phenylethylformamide (3.8%). Odorous aromatic derivatives represented 11.5% of the volatile fraction. Observed sulphur deriv...

Pagine di micologia, 14, 55-61 (2000).

The leaves of Argania spinosa from Morocco were investigated for flavonoids and condensed tannins. Four flavonol glycosides were identified by 1H NMR as myricitrin, quercitrin, hyperoside and myricetin 3-O-galactoside. UV spectrophotometric and histochemical methods were carried out to quantify and locate flavonoids and condensed tannins from leave...

Untransformed root cultures and Agrobacterium rhizogenes induced root cultures (hairy roots) of Plantago lanceolata were investigated for caffeic acid glycoside esters, i.e. verbascoside (V) and plantamoside (P), by HPLC. Levels of V (6–12 mg.g–1 DW) and P (30–80 mg.g–1 DW) from untransformed and hairy root cultures were not modified by 0.1 mM (E)-...

Annales de la Société d’Horticulture et d’Histoire Naturelle de l’Hérault, 139 (1), 18-19 (1999).

Poster a. Réunion de la Section de Mycologie (Société Française de Microbiologie) et du Réseau de Mycologie (Lille, France, 21-22/01/1999). b. in : Actes du Colloque, Société Française de Microbiologie, p 35.

Poster Tahrouch S., Andary C., Rapior S., Mondolot L., Gargadennec A. et Fruchier A. Polyphenol investigation of Argania spinosa (Sapotaceae) endemic tree from Morocco. Acta bot. Gallica, 147, 225-232 (2000).

Abstract Untransformed root cultures and Agrobacterium rhizogenes induced root cultures (hairy roots) of Plantago lanceolata were investigated for caffeic acid glycoside esters, i.e. verbascoside (V) and plantamoside (P), by HPLC. Levels of V (6–12 mg.g–1 DW) and P (30–80 mg.g–1 DW) from untransformed and hairy root cultures were not modified by 0...

ABSTRACT The burrowing nematode Radopholus similis is one of the most damaging pathogens on banana plantations. The role of phenolics in plant defense responses to the nematode was histochemically and ultrastructurally investigated in susceptible and partially resistant cultivars. Histochemical observations of healthy roots revealed that high level...

Many studies have addressed the improvement of indole alkaloid production in Catharanthus roseus cell suspension culture, but only few of them were concerned with entire plants cultured in vitro. The aim of this work was to investigate alkaloid distribution and the influence of DMSO (dimethyl sulfoxide) on alkaloid accumulation in C. roseus seedlin...

Summary. We determined the localization of phenolic compounds in dormant seeds of two American species of Sesbania (Fabaceae) by a combination of histochemical and biochemical methods. Phenolics are located in different layers of the seed coat and in the subhilar parenchyma. We then investigated the release of these compounds by imbibing seeds of b...

Ribwort (Plantago lanceolata) contains two main caffeic acid glycoside esters, plantamoside and verbascoside. These two polyphenols were investigated in the aerial and underground parts of in vitro cultured ribworts. For the first time, it is reported that, whatever the age of this plant, plantamoside and verbascoside are concentrated in the roots...

Veraison is a key stage ofgrape berry de-velopment, and few studies have been made to identify the nature of the signal that initiates ripening . Hormones such as abscisic acid (ABA) and auxin have been studied in rela-tion to berry ripening. ABA increases in concentration dur-ing berry ripening, and treatments that delay this increase seem to dela...

We investigated the uptake of a synthetic amine (N- (methoxycarbonylethyl)-N-[2-(1H-indol-3-yl)-ethyl]-β-methyl alaninate) by seedlings of C. roseus cultivated in vitro, and its effects on indole alkaloid accumulation. Root and aerial part indole alkaloids were analysed by HPLC and TLC. The results showed rapid absorption in the roots, which starte...

a. 4th Colloquium on Plant Natural Products (Ottawa, Canada, 26-29/05/1998), 39. b. in : Actes du Colloque, 89-92 (1999).

Analysis of the productivity, germination and karyograms of new populations of P. albicans in Tunisia clearly confirms that germination strategies are correlated with an increasing aridity gradient and hexaploid or tetraploid and diploid cytotypes. Germination is synchronous for the seeds of the hexaploid populations in a semi-arid bioclimate with...

Analysis of the productivity, germination and karyograms of new populations of P. albicans in Tunisia clearly confirms that germination strategies are correlated with an increasing aridity gradient and hexaploid or tetraploid and diploid cytotypes. Germination is synchronous for the seeds of the hexaploid populations in a semi-arid bioclimate with...

A screening programme for the detection of new natural antifungal agents showed high activity of crude extracts of Pycnoporellus fulgens against both yeast and dermatophytes. Before conducting additional studies on these mycolytic actions, we assessed the genotoxicity of crude extracts by using the cytokinesis-blocked micronucleus assay. A crude ex...

Argania spinosa, an endemic species from western-south of Morocco, was investigated for volatile components using Gas Chromatography/Mass Spectrometry. As far as we know, it is the first time that volatile constituents of A. spinosa are identified. Thirty volatile compounds were detected from leaves, pulp and kernel of fruit. The main constituents...

Annales de la Société d’Horticulture et d’Histoire Naturelle de l’Hérault, 138 (2), 42-46 (1998).

Poster Fons F., Rapior S., Gueiffier A., Roussel J.L., Gargadennec A. et Andary C. (E)-p-coumaroyl-1-O--D-glucopyranoside accumulation in roots of Plantago lanceolata cultures. Acta bot. Gallica, 145, 249-255 (1998). . Fons F., Tousch D., Rapior S., Gueiffier A., Roussel J.L., Gargadennec A. et Andary C. Phenolic profiles from untransformed and...

Poster a. 4th Colloquium on Plant Natural Products (Ottawa, Canada, 26-29/05/1998), 49-50. b. in : Actes du Colloque, 92-98 (1999).

Biotransformation of 1 mM (E)-cinnamic acid by thirty-day-old whole ribworts (Plantago lanceolala) was investigated. After addition of this caffeic acid precursor in medium culture, the root accumulation of the two main caifeic acid glycoside esters (CGEs), plantamoside and verbascoside was compared with in vitro ribwort control. No major modificat...

Fresh and dry Peganum harmala from Morocco were investigated for volatile components. Thirty-one volatile constituents were identified from aerial organs (leaves and stems) and roots. The dry parts were qualitatively richer in volatiles than the fresh ones. The leaves contained more volatile molecules than stems and roots. The main constituents wer...

Fruits, leaves arid scapes of wild Plantago lanceolata (ribwort) were investigated for volatile compounds by GC/MS. Leaves of young ribworts (35-day-old) cultured in vitro were also analysed. The volatile compound proportion corresponds to 0.05%, 0.03% and 0.001% of fresh weight for fruits, leaves and scapes, respectively. Thirty-five and twenty-si...

Volatile components of Argania spinosa (Sapotaceae) / Les substances volatiles d'Argania spinosa (Sapotaceae) Les substances volatiles des feuilles, de la pulpe et des amandes des fruits de l'arganier (Argania spinosa) ont ete etudiees pour la premiere fois par chromatographie en phase gazeuse couplee a la spectrometrie de masse. Trente composes vo...

Argania spinosa, an endemic species from western-south of Morocco, was investigated for volatile components using Gas Chromatography/Mass Spectrometry. As far as we know, it is the first time that volatile constituents of A. spinosa are identified. Thirty volatile compounds were detected from leaves, pulp and kernel of fruit. The main constituents...

The Madagascar periwinkle, Catharanthus roseus, produces numerous indole alkaloids, several of which have important therapeutic activities. These secondary metabolites are among the most expensive drugs because of their low abundance in intact plants. We investigated the biotransformation of a synthetic product (N-(methoxycarbonylethyl)-N-(2-(1H-in...

a. Réunion de la Section de Mycologie (Société Française de Microbiologie) et du Réseau de Mycologie (Lyon, France, 23-24/01/1997), Affiche 16. b. in : Actes du Colloque, Société Française de Microbiologie, p 53.

Riv. Ital. EPPOS (Numéro Spécial - 15èmes Journées Internationales sur les Huiles essentielles), 603-606 (1997).

Mikol. Fitopatol. (S. Petersburg), 31(3), 61-66 (1997). - Aqueous and methanol extracts obtained from fruiting bodies of lignicolous basidiomycete mushrooms Pholiota squarrosa, Pholiota gummosa, Kühneromyces mutabilis, Pleurotus ostreatus and Armillaria mellea (Basidiomycetes) have been studied for content of free sugars, free amino acids, phenolic...

Pholiota gummosa, Pholiota squarrosa, Kuehneromyces mutabilis, Pleurotus ostreatus. Amino acids - Aqueous and methanol extracts obtained from fruiting bodies of lignicolous basidiomycete mushrooms Pholiota squarrosa, Pholiota gummosa, Kühneromyces mutabilis, Pleurotus ostreatus and Armillaria mellea (Basidiomycetes) have been studied for content of...

Interactions between cotton cotyledons and #Xanthomonas campestris$ pv. #malvacearum$ were examined. During an incompatible interaction, fluorescence microscopy revealed that flavonoid compounds accumulated within 10 h after inoculation. Electron micrographs showed ultrastructural modifications of cells that exhibited an intense fluorescence sugges...

Rapior S., Ceballos L., Mousain D., Andary C. et Bessière J.M. Les champignons supérieurs : Etude préliminaire de bioproduction de substances volatiles par cultures sur milieux solides et liquides. Riv. Ital. EPPOS (Numéro Spécial - 15èmes Journées Internationales sur les Huiles essentielles, 1996), 603-606 (published 1997). Key Word: Boletaceae,...

An ethnobotanical survey of reproductive behaviour in Vanuatu and an extensive literature search have resulted in the selection of five plant species (Asplenium nidus, Hemigraphis reptans, Homalanthus nutans, Dysoxylum gaudichaudianum, Pemphis acidula) used for purposes relating to human reproduction in that country. Preliminary screening was carri...

Twenty-five volatile compounds were identified by GC/MS from seven fresh wild mushrooms (Piptoporus betulinus, Oligoporus caesius, Amanita rubescens, Paxillus involutus, Suillus grevillei, Suillus luteus, Xerocomus subtomentosus—Basidiomycetes). The main volatile components were 1-octen-3-ol, 3-heptanone, limonene, (E)-2-heptenal, (E)-2-decenal, 4-...

Twenty-nine volatile substances often frozen mushrooms (Grifola frondosa, Clitocybe nebularis, Clitocybe odora, Tricholoma sulfureum, Agaricus bisporus, Amanita ovoidea, Clitopilus prunulus, Hebeloma radicosum, Pholiota apicrea, Boletus satanas, Basidiomycetes) from France have been identified by GC/MS. The main components of the fruit bodies were...

The fruit bodies of Cortinarius armillatus were investigated for polyols, tree sugars and amino acids, phenolic acids, alkaloids, nitrogen content compounds, glycosides and fungal toxins using thin-layer chromatography and qualitative chemical reactions. Arabitol, mannitol, trehalose, fructose, 4-hydroxybenzoic acid, cholin, alanine, proline, serin...

Mikol. Fitopatol. (S. Petersburg), 30 (4), 79-86 (1996). Carpophores of Nematoloma fasciculare, N. capnoides and N. sublateritium were screened by thin layer chromatography and qualitative chemical reactions in order to detect polyols, free sugards, amino acides, phenolic acids, glycosides, alkaloids, nitrogen-containing compounds and toxins. Arabi...