Clarice D.B. Amaral

• Professor
• Professor at Federal University of Paraná

The present study developed a catalysis-based method using a natural deep eutectic solvent (NADES) for ammonia derivatization via air-assisted liquid–liquid microextraction (AALLME), followed by gas chromatography-mass spectrometry (GC–MS) analysis. The method involved the catalyzed derivatization of alkaline ammonia through its reaction with isobu...

In this study, extraction methods using natural deep eutectic solvents (NADES) were proposed for the determination of arsenic (As), cadmium (Cd), and lead (Pb) in plant and food matrices. NADES are green and sustainable solvents with advantageous chemical properties for such applications. The NADES were prepared with different components, character...

Objective To evaluate the efficacy of photobiomodulation therapy (PBMT) and bethanechol chloride (BC) on the quantity and quality of saliva in patients undergoing radiotherapy (RT) for head and neck cancer. Methods Saliva samples were collected from patients before and after RT, who were treated with PBMT or BC. Clinical parameters, including sali...

A vital component of surface plasmon resonance (SPR) biosensors is the recognition element. It generates the sensor selectivity towards the target biomarker and plays an important role in defining the sensor’s analytical sensitivity. In serological diagnostics, recombinant proteins (purified antigens) have presented better selectivity compared to s...

Objective To evaluate the efficacy of photobiomodulation therapy (PBMT) and bethanechol chloride (BC) on the quantity and quality of saliva in patients undergoing radiotherapy (RT) for head and neck cancer. Methods Saliva samples were collected from patients before and after RT, who were treated with PBMT or BC. Clinical parameters, including sali...

Sample preparation for elemental determination remains challenging, complex, and environmentally problematic. Recent advancements have been achieved by utilizing deep eutectic solvents (DES) in conventional extraction methods applied to many matrices and this article provides an evaluation of these applications. The DES-based methods were evaluated...

Background: Wilson’s disease (WD) is an autosomal recessive disorder secondary to an ATP7B gene mutation, leading to copper accumulation. Early diagnosis is fundamental because it allows copper clearance, thus limiting disease progression. This study assesses whether salivary copper is increased in Wilson’s disease patients compared to healthy cont...

Objective: Assess the impact of photobiomodulation therapy (PBMT) on xerostomia, salivary flow rate (SFR) and composition in patients undergoing radiotherapy (RT) for head and neck cancer (HNC). Study Design: Thirty patients undergoing RT (65 Gy) for HNC were enrolled. Saliva and xerostomia evaluations collected pre- and post-PBMT-RT. PBMT involved...

This study presents a novel and efficient method for the extraction of Al, Ca, Cr, Cu, K, Mg, Mn, and Zn in vegetable oil samples using a Natural Deep Eutectic Solvent (NADES) as an extractor combined with microwave radiation (MW) in an emulsion system. The NADES prepared with choline chloride:oxalic acid:water (1:1:4 molar ratio) provided a high e...

In this work, an innovative ultrasound-assisted matrix solid-phase dispersion (UA-MSPD) method and microwave-assisted extraction (MAE) were applied with amino acids-based deep eutectic solvents (AADES) for the extraction of arsenic (As) from medicinal herbs. Multivariate optimization by Doehlert design (DD) was performed to determine the optimal ex...

A natural deep eutectic solvent (NADES) synthesised from malic acid, xylitol, and water was used as a solvent in ultrasound-assisted extraction (UAE) for the determination of As and Cd in fish and shellfish samples by inductively coupled plasma mass spectrometry. The formation of the solvent was confirmed by infrared spectroscopy analysis, which sh...

THE FEMALE WINNERS OF THE NOBEL PRIZE IN CHEMISTRY (1901 – 2020). This paper contemplates the scientific contributions of the seven winners, from the creation of the Nobel Prize to the year 2020. The laureates are Marie Curie, Irène Joliot-Curie, Dorothy Crowfoot Hodgkin, Ada Yonath, Frances Hamilton Arnold, Emmanuelle Marie Charpentier, and Jennif...

The principles of green analytical chemistry have led to the development of analytical procedures that are increasingly sustainable. Different metrics have been created for the evaluation of greenness, although determination of the green nature of new analytical methods remains challenging, including for extraction methods that involve the use of n...

In this work, formic acid was used as an extracting solvent in a fast and accurate procedure for Cd and Pb determination in biological tissue by graphite furnace atomic absorption spectrometry (GF AAS). The procedure consists of adding formic acid to the sample, followed by heating and simple dilution before the determination of the analytes by GF...

In this study, a microwave-assisted extraction (MAE) method based on the natural deep eutectic solvent (NADES) was developed for the Cd, Cu, Fe, Mn, and Zn determination in medicinal herb samples. The experimental conditions were optimized and the method parameters were evaluated using certified reference material. The NADES composed of choline chl...

Amino acid-based deep eutectic solvents (AADES) represent a new subclass of deep eutectic solvents (DES) in which at least one of the components must be an amino acid, offering advantages such as low toxicity, biodegradability and low cost. In this work, β-alanine was used as hydrogen bond acceptor (HBA) in the preparation of a total of 30 AADES mi...

The behaviors of internal standards, according to different flow rates of the cell collision gas (He), were studied for the determination of Cd, Pb, Pd, Pt, Rh, and Sn in samples of fish and mollusks by inductively coupled plasma mass spectrometry (ICP-MS). The elements Bi, Ge, In, Sc, and Y were selected as internal standards, considering their ma...

NATURAL DEEP EUTECTIC SOLVENTS (NADES) IN SAMPLE PREPARATION OF PHOSPHATE ROCK AND MINERAL SUPPLEMENT FOR ELEMENTARY DETERMINATION BY PLASMA-BASED TECHNIQUES. Natural deep eutectic solvents (NADES) based on xylitol, citric acid, and malic acid were synthesized and used in ultrasound-assisted extraction (UAE) and heating-bath extraction of phosphate...

Background: Schizophrenia (SCZ) presents complex challenges related to diagnosis and clinical monitoring. The study of conditions associated with SCZ can be facilitated by using potential markers and patterns that provide information to support the diagnosis and oral health. Methods: The salivary composition of patients diagnosed with SCZ (n = 50)...

Livro de Passatempos sobre a vida e obra da cientista Marie Curie. São 66 passatempos do tipo: caça-palavras, palavras-cruzadas, desenhos para colorir, jogo dos sete erros, labirintos, criptogramas, anagramas, associação.

Neste artigo relatamos a experiência do Projeto de Extensão Meninas e Mulheres nas Ciências (MMC) da Universidade Federal do Paraná em divulgar informações técnico-científicas por meio de materiais lúdico-educativos sobre a Covid-19, na perspectiva da Divulgação Científica, para o grande público. Dentre as ações educativas do projeto, foi produzido...

Chromium determination in different matrices is very important, whether due to the harmful or beneficial effects of this element. The quantification of chromium present at very low concentrations in foods and environmental samples requires the use of highly sensitive analytical techniques, such as inductively coupled plasma mass spectrometry (ICP-M...

Conducting polymers are, without question, outstanding materials. Their unique combination between metal-like, electrical, magnetic, and optical properties and the processability of polymeric materials consolidated their use in the electrochemical sciences over the past decades. However, the versatile character of these polymers combined with the a...

The complexity of aerodynamic particulate matter’s (PM) matrices poses a challenge for the extraction and quantification of metals, especially for analytes with low concentration. Aiming to solve this issue, a precise and accurate protocol with the ultrasound extraction combined with microwave radiation digestion (USMW), was applied to PM samples w...

In this work, a method employing coprecipitation procedure with magnesium hydroxide as a carrier for separation/preconcentration of arsenic(V), cadmium(II), chromium(III), cobalt(II), copper(II), manganese(VII), mercury(II), nickel(II), and vanadium(V) was developed. The method was applied in fish and shellfish samples, and the elements were determ...

Solvents with both low density and viscosity have the advantage of higher extraction efficiency due to lower diffusivity and consequently higher mass transfer. In this study, a mixture design was performed for the synthesis of three different natural deep eutectic solvents (NADES) using citric acid, malic acid, and xylitol. The optimized proportion...

Elemental determination for fertilizer analysis by optical spectrometric techniques is often prone to non-spectral interferences due to the high contents of easily ionizable elements (EIE’s). Here, we verify the use of internal standardization as a strategy for compensating this matrix effect during the As, Cd, and Pb contaminant determinations in...

Catalysts used in the combustion process inside vehicles, palladium (Pd) and platinum (Pt), can be released to the atmosphere and deposited in aquatic environments. Thus, a simple and environmentally friendly method was developed for simultaneous Pd and Pt determination by ICP OES in aqueous samples. Cloud point extractions (CPE) combined with a fa...

Natural deep eutectic solvents (NADES) based on malic acid-citric acid-water (MA-CA), xylitol-citric acid-water (Xyl-CA) and xylitol-malic acid-water (Xyl-MA) were prepared according to three different methods: controlled heating and stirring, ultrasound-assisted synthesis (UAS), and microwave-assisted synthesis (MAS), in each case employing a 1:1:...

Natural deep eutectic solvents (NADES) based on xylitol, citric acid, and malic acid were synthesized and were then characterized using infrared spectroscopy, thermogravimetry (TG), differential scanning calorimetry (DSC), also density and viscosity were measurements. The deep eutectic solvents were used as solvent in ultrasound-assisted extraction...

Copper nanomaterials are increasingly produced and researched for many consumer products. They have high reactivity and bactericidal properties making them important in anti‐fouling paints, so being directly introduced in aquatic ecosystems. However, studies about the NPs‐Cu behaviour in natural aquatic systems and the interaction with primary prod...

This work aimed the development of an analytical procedure using inductively coupled plasma mass spectrometry (ICP-MS) in four modes of operation for simple and fast determination of Al, Ag, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Na, Ni, Pb, Sb, Se, Tl, Hg, V, U, and Zn in fresh and sea water samples attending simultaneously four Brazil...

Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilizat...

Some inorganic impurities are toxic to human health even when present at low concentrations and therefore must be carefully monitored in products as continuous use drugs. This work aimed the development of a simple microwave-assisted digestion procedure for different types of drugs and excipients and the analytical determination of elemental impuri...

This study investigated the capability of High Matrix Introduction (HMI) strategy for analysis of dialysis solution and urine samples using inductively coupled plasma mass spectrometry. The use of HMI enables the direct introduction of urine samples and dialysis solutions 2-fold diluted with 0.14 mol L−1 HNO3. Bismuth, Ge, Ir, Li, Pt, Rh, Sc and Tl...

The selection of an analytical method featuring multielemental capability, wide linear range, and ease of operation would be ideal for rare earth element (REE) determination in complex samples. This work targets the determination of Ce, Dy, Er, Eu, Gd, Ho, La, Lu, Nd, Pr, Sc, Sm, Tb, Th, Tm, Y, and Yb in geological samples, fertilizer, and agricult...

A microwave-induced plasma optical emission spectrometer with N2-based plasma was combined with multimode sample introduction system (MSIS) for hydride generation (HG) and multielemental determination of As, Bi, Ge, Sb and Sn in samples of forage, bovine liver, powered milk, agricultural gypsum, rice and mineral fertilizer, using a single condition...

Reports from the US media have raised the attention towards a possible contamination of apple juices by As. The study here described presents the development of an analytical procedure using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) for determination of As in apple and orange juices commercialized in the Tetra Pack® package. T...

A methodology was developed for the determination of vanadium in water samples from a hydromineral spa located in Ibirá, Sao Paulo State, Brazil and in bottled drinking water, employing inductively coupled plasma mass spectrometry (ICP-MS). Evaluation of matrix isobaric interferences focused on the ³⁵Cl¹⁶O⁺ polyatomic species at m/z 51. The determi...

The reactivity and analytical performance of O2 as cell gas in inductively coupled plasma tandem mass spectrometry was investigated. Selected analytes in a wide mass range were divided in three groups according to their reactivity: G1 represents elements with high oxygen affinity (Ce, La, P, Sc, Ti, and Y), G2 contains elements that may partially r...

Microwave-assisted sample digestion using closed vessels is becoming the standard for trace analysis because contamination and losses can be better controlled. Gases are generated during digestion and there is an increment of the internal vessel pressure. Consequently, vessels venting may occur depending on the design of the vessel and the maximum...

The feasibility of internal standardization and the oxygen reaction in ICP-MS was studied for accurate determination of boron. Lithium, Be and Rh were systematically evaluated as internal standards (IS.) for B determination in plants by ICP-MS/MS using a single quadrupole mode and MS/MS mode with O-2 into a cell, where B and internal standard signa...

In the present work, an inductively coupled plasma-tandem mass spectrometer (ICP-MS/MS) operating in single quadrupole and mass shift modes were evaluated for As determination in mineral fertilizers and agricultural gypsum with high contents of rare earth elements. For MS/MS mode, oxygen was the gas introduced into the octopole reaction system (ORS...

The performance of an inductively coupled plasma optical emission spectrometer was evaluated employing Ar, Ba, Mg, and Ni as test elements. Short and long-term stability were excellent with relative standard deviations lower than 2%. The warm-up times in axial, radial, and synchronous vertical dual view modes were 2 min. The limits of detection and...

Inorganic elements present in pharmaceutical products were determined by inductively coupled plasma-mass spectrometer (ICP-MS), employing microwave acid digestion as a sample preparation method. The most abundant isotopes, ⁷⁵As⁺, ⁶⁵Cu⁺, ⁵²Cr⁺, ⁵⁸Ni⁺, ²⁰⁸Pb⁺, and ⁵¹V⁺, were determined in acyclovir ointment and its constituents. Elemental impurities...

This work demonstrates the potentiality of inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) on trace element analysis. Aiming Hg determination in water analysis, sample preservation requires chloride addition, which presents formation of polyatomic interfering ions as a drawback for determination of other analytes (e.g. As and V). Th...

The feasibility of inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) to overcome polyatomic interferents in severely affected isotope determination is demonstrated here. This instrument arrangement presents an octopole reaction/collision system (ORS) in between two quadrupole mass analyzers. The first quadrupole operates as a mass fil...

Different conditions of extraction using water, a methanol–water mixture and nitric acid solutions were evaluated for speciation of As(III), As(V), DMA and MMA in plant samples that previously received As(V) after being sown and emergence was investigated. Microwave-assisted extraction (MAE) using diluted nitric acid solutions was also performed fo...

The objective of this work is to study the dynamics of nanoparticle formation by atomization of saline solutions at different concentrations. The nanoparticles were generated by a two fluid particle atomizer. Saline solutions were atomized and, with the immediate evaporation of the dispersion liquid in the droplets, resulted in an aerosol of solid...

2) Pesquisador da Embrapa Pecuária Sudeste, CP 339, CEP 13560-970, São Carlos, SP.; (3) Analista da Embrapa Pecuária Sudeste, CP 339, CEP 13560-970, São Carlos, SP. Resumo – O objetivo do trabalho foi estabelecer o teor crítico de contaminantes, com base nos resultados de produção de matéria seca de Brachiaria brizantha Stapf cv. Marandu após adiçã...