
Clara PosnerUniversity of California, San Diego | UCSD · Department of Bioengineering
Clara Posner
Bachelor of Science
About
16
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Introduction
I am a current PhD Candidate in the UC San Diego Bioengineering Department. I use genetically encoded fluorescent biosensors to investigate spatiotemporal signaling events in live cells under the guidance of Professor Jin Zhang in the UC San Diego Pharmacology Department.
Publications
Publications (16)
Recent advancements in optical metamaterials have opened new possibilities in the exciting field of super‐resolution microscopies. The far‐field metamaterial‐assisted illumination nanoscopies (MAINs) have, very recently, enhanced the lateral resolution to one‐fifteenth of the optical wavelength. However, the axial localization accuracy of fluoropho...
Cells execute specific responses to diverse environmental cues by encoding information in distinctly compartmentalized biochemical signalling reactions. Genetically encoded fluorescent biosensors enable the spatial and temporal monitoring of signalling events in live cells. Temporal and spatiotemporal computational models can be used to interpret b...
A major limitation of time-lapse microscopy combined with fluorescent biosensors, a powerful tool for quantifying spatiotemporal dynamics of signaling in single living cells, is low-experimental throughput. To overcome this limitation, we created a highly customizable, MATLAB-based platform: flexible automated liquid-handling combined microscope (F...
In this Letter, we propose a new quantitative phase imaging methodology named Fourier optical spin splitting microscopy (FOSSM). FOSSM relies on a metasurface located at the Fourier plane of a polarized microscope to separate the object image into two replicas of opposite circularly polarized states. The bias retardation between the two replicas is...
Time-lapse microscopy combined with genetically encoded fluorescent biosensors is a powerful tool for quantitatively measuring live cell signaling dynamics with high spatial and temporal resolution in single cells, providing rich signaling information beyond population-based biochemical assays and plate reading measurements. One major limitation, h...
Organic Hyperbolic Materials
In article number 2102230, Yeon Ui Lee, Zhaowei Liu, and co‐workers develop a structured illumination microscopy technique by using a low loss natural organic hyperbolic material (OHM). This OHM can support record high spatial‐frequency with an effective refractive index larger than 50 at visible frequencies. OHMs based...
Resolution capability of the linear structured illumination microscopy (SIM) plays a key role in its applications in physics, medicine, biology, and life science. Many advanced methodologies have been developed to extend the resolution of structured illumination by using subdiffraction‐limited optical excitation patterns. However, obtaining SIM ima...
Structured illumination microscopy (SIM) is one of the most powerful and versatile optical super-resolution techniques. Compared with other super-resolution methods, SIM has shown its unique advantages in wide-field imaging with high temporal resolution and low photon damage. However, traditional SIM only has about 2 times spatial resolution improv...
Determining the axial position of an emitter with nanoscale precision is critical to a fundamental imaging methodology. While there are many advanced optical techniques being applied to high-resolution imaging, high-axial-resolution topography imaging of living cells is particularly challenging. Here, we present an application of metamaterial-assis...
The dynamics of photons in fluorescent molecules plays a key role in fluorescence imaging, optical sensing, organic photovoltaics, and displays. Photobleaching is an irreversible photodegradation process of fluorophores, representing a fundamental limitation in relevant optical applications. Chemical reagents are used to suppress the photobleaching...
We developed a wide field of view super resolution microscopy technology, i.e. speckle metamaterial-assisted illumination nanoscopy (speckle-MAIN), showing biological imaging with resolution down to 40nm.
Genetically encoded Förster Resonance Energy Transfer (FRET)-based biosensors are powerful tools to illuminate spatiotemporal regulation of cell signaling in living cells, but the utility of the red spectrum for biosensing was limited due to a lack of bright and stable red fluorescent proteins. Here, we rationally improve the photophysical characte...
The lung is a relatively quiescent organ during homeostasis, but has a remarkable capacity for repair after injury. Alveolar epithelial type I cells (AEC1s) line airspaces and mediate gas exchange. After injury, they are regenerated by differentiation from their progenitors - alveolar epithelial type II cells (AEC2s) - which also secrete surfactant...