Claire E J Cheetham

Claire E J Cheetham
University of Pittsburgh | Pitt · Department of Neurobiology

PhD King's College London UK

About

49
Publications
3,419
Reads
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572
Citations
Additional affiliations
August 2017 - present
University of Pittsburgh
Position
  • Professor (Assistant)
February 2015 - July 2017
Carnegie Mellon University
Position
  • Professor
January 2012 - January 2015
National Institutes of Health
Position
  • Human Frontier Science Program Long-Term Fellow

Publications

Publications (49)
Article
For many organisms, certain odorants trigger instinctive responses that are essential for survival. A new study shows that mixing odorants interferes with this innate valence, demonstrating that innate odor information does not follow a privileged path through the brain.
Article
Age is the greatest risk factor for Parkinson's disease (PD) which causes progressive loss of dopamine (DA) neurons, with males at greater risk than females. Intriguingly, some DA neurons are more resilient to degeneration than others. Increasing evidence suggests that vesicular glutamate transporter (VGLUT) expression in DA neurons plays a role in...
Preprint
Full-text available
Postnatal neurogenesis provides an opportunity to understand how newborn neurons functionally integrate into circuits to restore lost function. Newborn olfactory sensory neurons (OSNs) wire into highly organized olfactory bulb (OB) circuits throughout life, enabling lifelong plasticity and regeneration. Immature OSNs can form functional synapses ca...
Preprint
Age is the greatest risk factor for Parkinson’s disease (PD) which causes progressive loss of dopamine (DA) neurons, with males at greater risk than females. We found that vesicular glutamate transporter (VGLUT) expression mediates vulnerability to age-related DA neurodegeneration in a sex-dependent manner, providing a new mechanism for sex differe...
Article
Full-text available
Dendritic spines are small protrusions on dendrites that endow neurons with the ability to receive and transform synaptic input. Dendritic spine number and morphology are altered as a consequence of synaptic plasticity and circuit refinement during adolescence. Dendritic spine density (DSD) is significantly different based on sex in subcortical bra...
Article
Voltage sensitive fluorescent dyes (VSDs) are important tools for probing signal transduction in neurons and other excitable cells. The impact of these highly lipophilic sensors has, however, been limited due to the lack of cell-specific targeting methods in brain tissue or living animals. We address this key challenge by introducing a nongenetic m...
Chapter
Neocortical circuits sit hierarchically atop somatosensory, visual, and auditory sensory processing in the mammalian brain. These circuits are complicated because each cortical area contains a vast range of excitatory and inhibitory cell types and each cell type integrates a variety of local and long-range input. Unraveling this circuitry is essent...
Article
Full-text available
Olfactory sensory neurons (OSNs) are generated throughout life from progenitor cells in the olfactory epithelium. OSN axons project in an odorant receptor-specific manner to the olfactory bulb (OB), forming an ordered array of glomeruli where they provide sensory input to OB neurons. The tetracycline transactivator (tTA) system permits developmenta...
Preprint
Full-text available
Voltage sensitive fluorescent dyes (VSDs) are important tools for probing signal transduction in neurons and other excitable cells. These sensors, rendered highly lipophilic to anchor the conjugated p-wire molecular framework in the membrane, offer several favorable functional parameters including fast response kinetics and high sensitivity to memb...
Article
Multiphoton intravital calcium imaging is a powerful technique that enables high-resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2-photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain. The prot...
Article
Full-text available
Significance statement: The identification of cell type-selective molecular markers has fostered tremendous insight into how distinct interneurons shape sensory processing and behavior. In the main olfactory bulb, inhibitory circuits precisely regulate the activity of principal cells to drive olfactory-guided behavior. Selective markers for main o...
Article
Full-text available
The mammalian olfactory bulb (OB) displays robust activity-dependent plasticity throughout life. Dopaminergic (DA) neurons in the glomerular layer (GL) of the OB are particularly plastic, with loss of sensory input rapidly reducing tyrosine hydroxylase (TH) expression and dopamine production, followed by a substantial reduction in DA neuron number....
Article
Full-text available
Incorporation of new neurons enables plasticity and repair of circuits in the adult brain. Adult neurogenesis is a key feature of the mammalian olfactory system, with new olfactory sensory neurons (OSNs) wiring into highly organized olfactory bulb (OB) circuits throughout life. However, neither when new postnatally generated OSNs first form synapse...
Data
Mature OSN axons innervating a single glomerulus in a juvenile OMP-sypGFP-tdTom mouse.
Data
Supplementary Figures 1-5, Supplementary Tables 1-3 and Supplementary References.
Data
Immature OSN axons innervating a single glomerulus in a juvenile Gγ8-sypGFP-tdTom mouse.
Article
Full-text available
The ability to label and manipulate specific cell types is central to understanding the structure and function of neuronal circuits. Here, we have developed a simple, affordable strategy for labeling of genetically-defined populations of neurons throughout a targeted brain region: Bulk Regional Viral Injection (BReVI). Our strategy involves a large...
Article
Full-text available
Synaptic neurotransmission is modified at cortical connections throughout life. Varying the amplitude of the postsynaptic response is one mechanism that generates flexible signaling in neural circuits. The timing of the synaptic response may also play a role. Here, we investigated whether weakening and loss of an entire connection between excitator...
Article
Full-text available
Longitudinal imaging studies of individual neurons in vivo have greatly enhanced our understanding of the rewiring that the adult brain is capable of, both during normal experience and in response to altered sensory input or learning. Most of these studies have focused on dendritic spines, tiny
Article
Full-text available
Mature neocortex adapts to altered sensory input by changing neural activity in cortical circuits. The underlying cellular mechanisms remain unclear. We used blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI) to show reorganization in somatosensory cortex elicited by altered whisker sensory input. We found that there w...
Article
Full-text available
A critical period of plasticity allows neuronal circuitry of the mammalian olfactory system to develop.
Article
Full-text available
Behavioral experience alters the strength of neuronal connections in adult neocortex. These changes in synaptic strength are thought to be central to experience-dependent plasticity, learning, and memory. However, it is not known how changes in synaptic transmission between neurons become persistent, thereby enabling the storage of previous experie...
Article
Full-text available
Behavioral experience alters the strength of neuronal connections in adult neocortex. These changes in synaptic strength are thought to be central to experience-dependent plasticity, learning, and memory. However, it is not known how changes in synaptic transmission between neurons become persistent, thereby enabling the storage of previous experie...
Article
Full-text available
Ocular dominance (OD) plasticity in the visual cortex is a classic model system for understanding developmental plasticity, but the visual cortex also shows plasticity in adulthood. Whether the plasticity mechanisms are similar or different at the two ages is not clear. Several plasticity mechanisms operate during development, including homeostatic...
Article
Full-text available
Non-technical summary The visual cortex and the somatosensory whisker barrel cortex are widely studied model systems of experience-dependent plasticity, which enables the brain to adapt to changes in the environment and is required for recovery in conditions such as stroke. It is known that presynaptic development of excitatory synapses in the cort...
Article
Full-text available
Experience-dependent cortical plasticity has been studied by using loss-of-function methods. Here, we take the complementary approach of using a genetic gain-of-function that enhances plasticity. We show that a constitutively active form of H-ras (H-ras(G12V)), expressed presynaptically at excitatory synapses in mice, accelerates and enhances multi...
Article
Full-text available
Visual and somatosensory cortices exhibit profound experience-dependent plasticity during development and adulthood and are common model systems for probing the synaptic and molecular mechanisms of plasticity. However, comparisons between the two areas may be confounded by a lack of accurate information on their relative rates of development. In th...
Article
Full-text available
Experience-dependent plasticity in adulthood is slower than during development. Previous experience can accelerate adult cortical plasticity. However, the contributions of functional synaptic changes and modifications in neuronal structure to the acceleration of adult cortical plasticity remain unclear. If structural remodeling was important then i...
Article
Full-text available
Neocortical circuitry can alter throughout life with experience. However, the contributions of changes in synaptic strength and modifications in neuronal wiring to experience-dependent plasticity in mature animals remain unclear. We trimmed whiskers of rats and made electrophysiological recordings after whisker cortical maps have developed. Measure...

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