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14
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Introduction
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July 2013 - December 2017
Publications
Publications (14)
Objective:
: The effects of carnosine synthesis on the structural and microstructural determinants of meat quality have not been studied to date. Therefore, this study aimed to investigate the effect of supplementation with carnosine synthesis precursors on the characteristics and microstructure of breast muscle fibers in slow-growing Korat chicke...
This study proposes a strategy to manipulate the fatty acid (FA) content in slow-growing Korat chicken (KRC) meat using tuna oil (TO). To determine the optimal level and feeding period of TO supplementation, we conducted a study investigating the effects of dietary TO levels and feeding periods on meat quality, omega-3 polyunsaturated fatty acid (n...
Carnosine enrichment of slow-growing Korat chicken (KRC) meat helps differentiate KRC from mainstream chicken. We aimed to investigate the effects of β-alanine and L-histidine supplementation on the carnosine synthesis in and quality and secondary structure of proteins in slow-growing KRC meat. Four hundred 21-day-old female KRC were used, and a co...
Improving feed efficiency is an important breeding target for the poultry industry; to achieve this, it is necessary to understand the molecular basis of feed efficiency. We compared the jejunal transcriptomes of low- and high-feed conversion ratio (FCR) slow-growing Korat chickens (KRs). Using an original sample of 75 isolated 10-week-old KR males...
In birds, oviductal cells play a crucial role in the storage of sperm via cell-to-cell communication including extracellular vesicles (EV). We developed a culture of oviductal organoids enriched in sperm storage tubules (SSTorg) to demonstrate the release of EV. SSTorg were cultured for 24h and added to live (LV), frozen (FZ) and lysed (LY) avian s...
Uterine fluid is an aqueous milieu to which sperm are exposed during their storage and ascent. In this study, a bottom‐up proteomic strategy and bioinformatic analysis of hen uterine fluid was performed to improve the understanding of this fluid and its potential role in sperm survival mechanisms. The proteomic data were submitted to ProteomeXchang...
Avian uterine fluid (UF) and uterovaginal sperm storage tubules (SST) are key components in accepting sperm in SST, maintaining sperm function for several weeks, releasing sperm from SST and their ascent through the uterus. To improve understanding of sperm storage processes requires investigating UF and SST. This study aimed to identify proteins m...
While uterine epithelium secretes eggshell matrix proteins to regulate eggshell structural organization, uterovaginal junction (UVJ) epithelium supports sperm storage in tubules (SST). Here, we examined the presence of OCX36, OC-116 and OC-17 eggshell matrix proteins in SSTs. Two experimental lines of hens displaying either a long (F+ line) or a sh...
About the avian uterine fluid proteome: exosomes significance and biological processes in regard to eggshell mineralization, immunity and reproduction
In avian species, sperm is stored for a long period in sperm storage tubules (SST) located in the utero-vaginal junction. It has been shown that progesterone (P4) regulates the release of sperm from SST for fertilization [Ito et al. 2011 Endocrinology 152, 3952–3621] in quails. In the same context, heat shock proteins (HSP) such as the HSP70 family...
The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the...
Hen’s Sperm Storage Tubes (SSP) new culture methodology using primary entire isolated tubes in culture suspention.
Because of prolonged sperm storage in their oviduct, domestic hens can produce fertile eggs for up to 3 weeks following a single AI. The oviduct secretions may have an effect on sperm survival, but its composition during fertilization is unknown. In the present study, we compared the proteomic content of uterine fluid collected from two lines of he...