Chwan-Yang Hong

Chwan-Yang Hong
National Taiwan University | NTU · Department of Agricultural Chemistry

Ph.D.

About

60
Publications
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Publications

Publications (60)
Article
Full-text available
Reactive oxygen species are thought to play an important role in NaCl stress. Therefore, the expression patterns of the gene family encoding the H2O2-scavenging enzyme ascorbate peroxidase (APx; EC1.11.1.11) were analysed in roots of etiolated rice (Oryza sativa L.) seedlings in response to NaCl stress. Applying semi-quantitative RT-PCR, the mRNA l...
Article
Full-text available
Gibberellic acid (GA) and abscisic acid (ABA) are the primary phytohormones that play antagonistic roles in the control of the transition from embryogenesis to germination in seeds (Finch‐Savage and Leubner‐Metzger, 2006). Given the low concentrations of GA and ABA in seeds and plants, biosynthesis inhibitors (Toh et al., 2008) or deficient mutants...
Article
Full-text available
In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable flu...
Article
Full-text available
Glutathione reductase (GR) is one of important antioxidant enzymes in plants. This enzyme catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH) with the accompanying oxidation of NADPH. Previously, we showed that salt-stress-responsive GR3 is a functional protein localized in chloroplasts and mitochondria in rice. To...
Article
The accumulation and effect of cadmium (Cd) on the growth and enzymatic activities changes of antioxidants in Tagetes patula, French marigold, were investigated to reveal the physiological mechanisms corresponding to its Cd tolerance and accumulation. Hydroponically grown T. patula plants were treated with different concentrations of Cd (0, 10, 25,...
Article
Full-text available
Key message The inhibitory effect of eugenol on rice germination is mediated by a two-step modulatory process: Eugenol first regulates the antagonism of GA and ABA, followed by activation of catalase activity. Abstract The natural monoterpene eugenol has been reported to inhibit preharvest sprouting in rice. However, the inhibitory mechanism remai...
Article
Full-text available
Plant CRM domain-containing proteins are capable of binding RNA to facilitate the splicing of group I or II introns in chloroplasts, but their functions in mitochondria are less clear. In the present study, Arabidopsis thaliana CFM6, a protein with a single CRM domain, was expressed in most plant tissues, particularly in flower tissues, and restric...
Chapter
Under natural conditions, plants are often exposed to both biotic (pathogens, wounding, insects) and abiotic stresses (drought, salinity, heat, high or low temperature, UV light, heavy metals, ozone) simultaneously. Among the various abiotic stresses, drought along with salinity and alkalinity confers osmotic stress and causes major yield losses, w...
Article
Full-text available
The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be considered for the efficient implementation of this technology. To generate genome-edited crops, single guide (sg)RNA and Cas9 DNA are delivered...
Article
Full-text available
Genome-editing techniques such as CRISPR/Cas9 have been widely used in crop functional genomics and improvement. To efficiently deliver the guide RNA and Cas9, most studies still rely on Agrobacterium-mediated transformation, which involves a selection marker gene. However, several limiting factors may impede the efficiency of screening transgene-f...
Article
Control of gene expression and induction of cellular protection mechanisms are two important processes that plants employ to protect themselves against abiotic stresses. ABA-, stress, and ripening-induced (ASR) proteins have been identified to participate in such responses. Previous studies have proposed that these proteins can act as transcription...
Article
Full-text available
Background: Many transcription factors (TFs), such as those in the basic helix-loop-helix (bHLH) family, are important for regulating plant growth and plant responses to abiotic stress. The expression of OsbHLH035 is induced by drought and salinity. However, its functional role in rice growth, development, and the salt response is still unknown....
Article
Full-text available
The florist’s Gloxinia, Sinningia speciosa, which bears considerable flower trait variations, is an emerging model plants to study floral traits development. However, the investigation of the genetic information linking these floral traits is limited due to a lack of a reliable and efficient transformation system for functional studies. This study...
Chapter
Full-text available
Abiotic stresses severely limit crop productivity. Plants being sessile, they are continuously exposed to a broad range of environmental stresses. Hence, multiple stress situations are more likely to occur in field conditions. Nevertheless, plants have evolved strategies to sense their environment to modulate its growth. However, its prime aim is t...
Chapter
Full-text available
Abiotic stress is a major factor impeding crop productivity globally. Almost all abiotic stresses induce the accumulation of reactive oxygen species and consequently cause oxidative stress. Glutathione (GSH) and glutathione reductase (GR) are important components of the antioxidant machinery that plants use to respond to abiotic stress. GR catalyze...
Article
Rice (Oryza sativa L.) is a salt-sensitive species. Salt stress can cause injury to plant cellular membrane. Plant lipid transfer proteins (LTPs) are abundant lipid binding proteins that are important in membrane vesicle biogenesis and trafficking, however, the biological importance of LTPs on salt-stress response in rice remains unclear. Therefore...
Article
Full-text available
An abundant 17 kDa RNase, encoded by OsPR10a (also known as PBZ1), was purified from Pi-starved rice suspension-cultured cells. Biochemical analysis showed that the range of optimal temperature for its RNase activity was 40-70°C and the optimum pH was 5.0. Disulfide bond formation and divalent metal ion Mg2+ were required for the RNase activity. Th...
Data
OsPR10a genomic DNA sequence. (PDF)
Data
Purification and characterization of OsPR10a protein expressed in E. coli. (PDF)
Data
RT-PCR analysis of pathogen resistant genes in WT and OsPR10a-overexpressing lines. (PDF)
Data
Phenotype of WT and Arabidopsis OsPR10a-overexpressing grown in +Pi and –Pi conditions. (PDF)
Data
Biomass of rice suspension-cultured cells growth under normal and nutritional starvation of culture media. (PDF)
Data
RT-PCR analysis of pathogen resistant genes in WT (Col-0) and OsPR10a-overexpressing lines of Arabidopsis. (PDF)
Data
Hydroponic culture of OsPR10a-overexpressing rice seedlings under +Pi and –Pi conditions. (PDF)
Data
Comparison of the phenotypes of the WT and the transgenic lines under normal growth conditions. (PDF)
Data
Enhanced disease resistance against Xoo challenge under -Pi conditions in WT rice seedlings. (PDF)
Data
Southern blot analysis of Ubi::OsPR10a transgenic lines. (PDF)
Data
Phenotypes of WT and OsPR10a-overexpressing transgenic lines under +Pi and –Pi conditions. (PDF)
Data
Amino acids sequence of OsPR10a. (PDF)
Article
Full-text available
Background: Anthocyanin accumulates in many plant tissues or organs, in rice for example leading to red, purple red and purple phenotypes for protection from damage by biotic and abiotic stresses and for reproduction. Purple leaf, leaf sheath, stigma, pericarp, and apiculus are common in wild rice and landraces and occasionally found in modern cul...
Article
Full-text available
The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mechanism behind Hyg-induced cell...
Article
Screening salt-sensitive mutants is a powerful method to identify genes associated with salt tolerance. We used forward genetic screening with sodium azide-mutated rice (Oryza sativa L. cv. Tainung 67) to identify mutants showing hypersensitivity to salt stress. A new mutant line, named salt hypersensitive 1 (shs1) and exhibiting a severe salt-sens...
Article
Conazole pollution is an emerging concern to human health and environmental safety because of the broad use of conazole fungicides in agriculture and medicine and their frequent occurrence in aquifers. The agricultural pesticide propiconazole has received much regulatory interest because it is a known rodent carcinogen with evidence of multiple adv...
Article
Our previous study demonstrated that purified Glycine max β-glucosidase (GmBGL) could hydrolyze glucosyl isoflavone to the aglyconic form. In this study, we report the cloning and functional characterization of a soybean cDNA encoding the β-glucosidase. GmBGL was isolated by use of a purified soybean N-terminal amino acid sequence and conserved seq...
Article
Full-text available
Rice is a major food source for much of the world, and expanding our knowledge of genes conferring specific rice grain attributes will benefit both farmer and consumer. Here we present novel dull grain mutants with a low amylose content (AC) derived from mutagenesis of Oryza sativa, ssp. japonica cv. Taikeng 8 (TK8). Positional cloning of the gene...
Article
Vinclozolin, a dicarboximide fungicide, is an endocrine disrupting chemical that competes with an androgenic endocrine disruptor compound. Most research has focused on the epigenetic effect of vinclozolin in humans. In terms of ecotoxicology, understanding the effect of vinclozolin on non-target organisms is important. The expression profile of a c...
Article
Full-text available
By oligo microarray expression profiling, we identified a rice RING zinc-finger protein (RZFP), OsRZFP34, whose gene expression increased with high temperature or abscisic acid (ABA) treatment. As compared with the wild type, rice and Arabidopsis with OsRZFP34 overexpression showed increased relative stomata opening even with ABA treatment. Further...
Article
The rice stunted lemma/palea 1 (slp1) mutant displays a dwarf, shortened panicle length, degenerated lemma and palea, and sterility. A previous study suggested that a missense mutation at the sixth amino acid of the OsSPL16 protein was likely to be responsible for the slp1 mutant phenotype. The current study shows that the overexpression of the wil...
Article
Full-text available
Deadenylation, also called poly(A) tail shortening, is the first, rate-limiting step in the general cytoplasmic mRNA degradation in eukaryotic cells. The CCR4-NOT complex, containing the two key components carbon catabolite repressor 4 (CCR4) and CCR4-associated factor 1 (CAF1), is a major player in deadenylation. CAF1 belongs to the RNase D group...
Article
Key message: Rice ASR genes respond distinctly to abscisic acid, dehydration and cold stress. Their tissue-specific expression provides new hints about their possible roles in plant responses to stress. Plant ASR proteins have emerged as an interesting distinct group of proteins with apparent roles in protecting cellular structures as well as puta...
Article
Full-text available
IIn the root of rice (Oryza sativa L.), ABA treatment, salinity or water-deficit stress induces the expression of a family of four genes, Repetitive Proline Rich Protein (RePRP). These genes encode two subclasses of novel proline-rich glycoproteins with highly repetitive PX1PX2 motifs, RePRP1 and RePRP2. RePRP orthologues exist only in monocotyledo...
Article
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Glutathione reductases (GRs) are important components of the antioxidant machinery that plants use to respond against abiotic stresses. In rice, one cytosolic and two chloroplastic GR isoforms have been identified. In this work, we describe the cloning and characterization of the full-length cDNA encoding OsGR3, a chloroplast-localized GR that up t...
Article
Full-text available
Background Actin depolymerizing factors (ADFs) are small actin-binding proteins. Many higher-plant ADFs has been known to involve in plant growth, development and pathogen defense. However, in rice the temporal and spatial expression of OsADF gene family and their relationship with abiotic stresses tolerance is still unknown. Results Here we repor...
Article
Full-text available
Lateral root (LR) development performs the essential tasks of providing water, nutrients, and physical support to plants. Therefore, understanding the regulation of LR development is of agronomic importance. In this study, we examined the effect of nitric oxide (NO), auxin, and hemin (Hm) on LR formation in rice. Treatment with Hm [a highly effecti...
Article
Full-text available
Cadmium (Cd) is readily taken up by the roots of rice seedlings, leading to growth reduction. H2O2 is a constituent of oxidative metabolism and is itself a reactive oxygen species. In this study, the participation of H2O2 in CdCl2-inhibited growth of rice roots was investigated. CdCl2 treatment increased H2O2 production in rice roots. CdCl2 treatme...
Article
Full-text available
Cadmium (Cd)-sensitive and -tolerant rice cultivars were used to study the role of reduced glutathione (GSH) in Cd-induced toxicity. Cd toxicity of rice seedlings was evaluated by the decrease in chlorophyll content and the increase in malondialehyde content in the second leaves. On treatment with 5 μM CdCl 2 for 6 days, the content of GSH decrease...
Article
Tagetes patula, a popular garden species, is a newly discovered Cd-hyperaccumulator with the advantages of broad environmental adaptablilty, high biomass and high accumulating capability to Cd. To investigate the physiological changes to Cd, hydroponic culture system was developed. Expose of plants to 5 and 10 μM CdCl2 for 14 days results in accumu...
Article
Cadmium (Cd) toxicity of rice (Oryza sativa L. cv. Taichung Native 1) seedlings was evaluated by the decrease in chlorophyll content and the increase in malondialdehyde (MDA) in the second leaves of rice seedlings. CdCl2 (5 microM) treatment was accompanied by a decrease in the contents of ascorbic acid (AsA) and AsA + dehydroascorbate (DHA) and in...
Article
Full-text available
Reactive oxygen species (ROS) play an important role in NaCl stress. Plants tolerant to NaCl stress may evolve certain strategies to remove these ROS, thus reducing their toxic effects. Therefore, the expression patterns of the gene family encoding glutathione reductase (GR, EC 1.6.4.2) were analyzed in roots of etiolated rice (Oryza sativa L.) see...
Article
Glutathione reductase (GR, EC 1,6.4.2) is an important reactive oxygen species-scavenging enzyme. The present study examined the relative importance of Na(+), Cl(-), and the osmotic component in NaCl-induced expression of Oryza sativa glutathione reductase (OsGR) genes in rice roots. Semi-quantitative RT-PCR was used to quantify the mRNA levels for...
Article
Full-text available
Ascorbate peroxidase (APx; EC 1.11.1.11) plays an important role in scavenging the toxic effects of H(2)O(2) in higher plants. Eight types of APx have been described for Oryza sativa: two cytosolic (OsAPx1 and OsAPx2), two putative peroxisomal (OsAPx3 and OsAPx4), and four chloroplastic isoforms (OsAPx5, OsAPx6, OsAPx7 and OsAPx8). We have recently...
Article
Full-text available
Promoters play key roles in conferring temporal, spatial, chemical, developmental, or environmental regulation of gene expression. Promoters that are subject to specific regulations are useful for manipulating foreign gene expression in plant cells, tissues, or organs with desirable patterns and under controlled conditions, and have been important...
Article
The accumulation of H2O2 by NaCl was observed in the roots of rice seedlings. Treatment with NaCl caused an increase in the activities of ascorbate peroxidase (APX) and glutathione reductase (GR) and the expression of OsAPX and OsGR in rice roots. Exogenously applied H2O2 also enhanced the activities of APX and GR and the expression of OsAPX and Os...
Article
The present study examined the response of antioxidant systems to NaCl stress and the relative importance of Na+ and Cl– in NaCl-induced antioxidant systems in roots of rice seedlings. NaCl treatment caused an increase in the activities of ascorbate peroxidase (APX) and glutathione reductase (GR) in roots of rice seedlings, but had no effect on the...
Article
Full-text available
Phytate is the main storage form of phosphorus in many plant seeds, but phosphate bound in this form is not available to monogastric animals. Phytase, an enzyme that hydrolyzes phosphate from phytate, has the potential to enhance phosphorus availability in animal diets when engineered in rice seeds as a feed additive. Two genes, derived from a rumi...

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