Chunfang Wang

Huazhong Agricultural University | HZAU · College of Fisheries

In order to evaluate the effects of the interaction between different proteins and feeding frequency on largemouth bass (Micropterus salmoides) and to provide scientific guidance for the application of novel proteins and the corresponding optimal feeding strategy, a two-factorial design (5 × 3) with five protein feeds (fishmeal (FM), Clostridium au...

The land-based recirculating aquaculture system (RAS) has been widely applied to fish farming as a new eco-friendly culture model. This system consists of circular culture tanks on land integrated with water treatment and recycling systems. This study investigated the growth performance of juvenile gibel carp (Carassius gibelio) cultured at high st...

Several nutrients are crucial in enhancing the immune system and preserving the structural integrity of bodily tissue barriers. Vitamin D (VD) and zinc (Zn) have received considerable interest due to their immunomodulatory properties and ability to enhance the body’s immune defenses. Due to their antiviral, anti-inflammatory, antioxidative, and imm...

Vitamin D receptors (VDR) and vitamin D3 metabolizing enzymes have been found to be highly expressed in the ovaries and spermatophores of fish. However, the role of VD3 on fish gonadal development has rarely been reported. In this study, two-month-old female zebrafish were fed with different concentrations of VD3 diets (0 IU/kg, 700 IU/kg, 1400 IU/...

Vitamin D (VD) is a steroid hormone, of which vitamin D3 (VD3) is the main form in fish. Fish obtain VD3 majorly from diets, and VD3 has been widely used in aquatic feed as feed additive. As one of the essential nutrients, VD3 plays an indispensable role in the growth and health of fish. Due to the particularity of the fish living environment, the...

Introduction In an effort to minimize the usage of fishmeal in aquaculture, novel protein diets, including Tenebrio molitor, cottonseed protein concentrate, Clostridium autoethanogenum, and Chlorella vulgaris were evaluated for their potential to replace fishmeal. Nevertheless, comprehensive examinations on the gut health of aquatic animals under a...

In order to solve a series of problems in the current feeding process, such as inaccuracy, unevenness and high labour costs, a precision feeding system for largemouth bass was developed by integrating feeding management model, water quality monitoring system, fish feeding activity sensor, automatic feeding machine and software platform. Based on th...

Precise feeding is an effective guarantee to improve the welfare of aquatic animals, increase culture efficiency and reduce environmental pollution, whereas internet of things (IoT) technology is an important means to promote the development of informatization and intelligence of aquaculture. In order to improve the precision, informatization and i...

The novel proteins, i.e., Clostridium autoethanogenum (CAP), Tenebrio molitor (TM), Chlorella vulgaris (ChM), and cottonseed protein concentrate (CSM), are promising food for the future aquaculture due to the limited fishmeal (FM) resources. However, in-depth investigations on the intestinal barriers of fish when fed novel proteins are sparse, and...

To investigate the possibility of completely replacing dietary fish meal with several novel proteins in largemouth bass (Micropterus salmoides), the intestinal health of largemouth bass switched to novel protein diets after being fed with fish meal diet for a certain time were examined. The novel proteins included Chlorella vulgaris (ChM), Clostrid...

Ferroptosis is a kind of iron-dependent programed cell death. Vitamin D has been shown to be an antioxidant and a regulator of iron metabolism, but the relationship between vitamin D and ferroptosis is poorly studied in fish. This study used zebrafish liver cells (ZFL) to establish a ferroptosis model to explore the effect of 1,25(OH)2D3 on cell fe...

Background: Smartphones have captured the attention of many researchers in the field of health and medical services. This study aimed to quantify research ‘hotspots’ in this field, analyse the relationship between research hotspots and the resulting knowledge groups, and provide visual representations of the findings. Methods: Using bibliometric an...

Grass carp, Ctenopharyngodon idella, was introduced to the southern United States in the 1960s to control aquatic macrophytes in aquaculture ponds. Accidental release of grass carp from some research facilities and aquaculture farms at the start of the introduction, followed by broad promotion of stocking triploid and diploid fish helped to facilit...

Vitamin D3 (VD3) has been shown to modulate the innate immune response in mammals but this has been rarely reported in fish. The current study found that increasing dietary VD3 content can reduce the density of yellow to dark brown pigmented macrophage aggregates (PMAs) in the spleens of yellow catfish infected with Edwardsiella ictaluri. The resul...

Vitamin D3 (VD3) has been shown to regulate immune function in mammals. 1,25-dihydroxyvitamin(1,25(OH)2D3) is the active form of vitamin D3, which is also known as calcitriol. The current study investigated the immunomodulatory effects of 1,25(OH)2D3 on the innate immune response of yellow catfish (Pelteobagrus fulvidraco) after in vivo and in vitr...

Edwardsiella ictaluri (E. ictaluri) causes severe infections in yellow catfish (Pelteobagrus fulvidraco), which leads to a massive loss in the aquaculture industry especially in catfish commercial production. Previous studies have confirmed that vitamin D3 is essential in immune regulation in mammals. Based on next-generation sequencing, this study...

In order to predict the growth performance, feed requirement and waste output, and improve the precision of feeding management, the current study reported some bioenergy models developed in grass carp (Ctenopharyngodon idella). In this study, the growth rate of C. idella at different growth stages was calculated by specific growth rate (SGR), daily...

In this study, two isoforms slc34a2 genes (type IIb sodium-dependent phosphate cotransporter), slc34a2a2 and slc34a2b, were cloned from intestine and kidney of yellow catfish (Pelteobagrus fulvidraco), with rapid amplification of cDNA ends. The structure differences and the regulation effects of dietary VD3 under low phosphorus were compared among...

The study was carried out to investigate the effects on possible gene regulation in intestine and kidney of yellow catfish (Pelteobagrus fulvidraco) under the treatments of different dietary Vitamin D3 levels (0(VD0), 1243(VD2), 22 700(VD20) IU/Kg) by RNA-seq technique. 83 265 unigenes were selected from the 305 568 982 raw reads. The average lengt...

A 70-day starvation was conducted to determine the effects of long-term starvation on whole body polyunsaturated fatty acid (PUFA) composition and fatty acid metabolism-related gene expression in the liver of zebrafish, Danio rerio. Starvation period was followed by a 15-day refeeding period to test recovery both PUFA composition and gene expressio...

A 70-day starvation was conducted to determine the effects of long-term starvation on whole body polyunsaturated fatty acid (PUFA) composition and fatty acid metabolism-related gene expression in the liver of zebrafish, Danio rerio. Starvation period was followed by a 15-day refeeding period to test recovery both PUFA composition and gene expressio...

A sodium-dependent phosphate cotransporter gene, NaPi-IIb (slc34a2), was isolated from yellow catfish (Pelteobagrus fulvidraco) intestine through homology cloning and the rapid amplification of cDNA ends. The full-length cDNA of slc34a2 consisted of 2326 bp with an open reading frame encoding 621 amino acids, a 160-bp 5' untranslated region, and a...

An 8-week feeding trial was conducted to evaluate the sparing effect of dietary phytase on decreasing supplement of dietary sodium dihydrogen phosphate (NaH2PO4) in the diets of juvenile yellow catfish Pelteobagrus fulvidraco. Seven diets were formulated: one reference group that contained only 2% NaH2PO4; one control group that consisted of neithe...

We conducted a growth trial to study how dietary phosphorus (P) influenced utilization and excretion of P for juvenile yellow catfish. Four mathematical models describing different mechanisms of fish phosphorus metabolism were tested, and the best model was selected using Akaike information criterion to describe the relationships between dietary Pa...

This experiment was conducted to evaluate the combined effects of phytase, citric acid and vitamin D3 on the digestibility coefficients, growth performance, body composition, and vertebral and serum mineral contents in juvenile yellow catfish (Pelteobagrus fulvidraco). The study used 1200 juvenile yellow catfish in a completely randomized design wi...

A growth trial was conducted to detect the effects of different diets on the growth performance and hypoxia adaptation capacity of Mississippi Paddlefish (Polyodon spathula) larvae. The larvae were fed with live food, formulated diets, and 1/2 live food with 1/2 formulated diets. After a 15-d growth trial, final body weight and total body length we...

Walleye (Sander vitreus) is an important sport fish throughout much of North America, and walleye populations support valuable commercial fisheries in certain lakes as well. Using a corrected algorithm for balancing the energy budget, we reevaluated the performance of the Wisconsin bioenergetics model for walleye in the laboratory. Walleyes were fe...

An 8‐week feeding trial was carried out using young hybrid sturgeon (♀Huso huso × ♂Acipenser schrenckii) to study the effects of dietary phosphorus (P) on growth performance, body composition, liver and serum antioxidant status as well as the effluent P content in the wintertime. Four puffed pellet diets were formulated to contain graded total P le...

An 8-week feeding trial was carried out with juvenile yellow catfish to study the effects of dietary available phosphorus (P) on growth performance, body composition, and hepatic antioxidant property. Six pellet diets were formulated to contain graded available P levels at 0.33, 0.56, 0.81, 1.15, 1.31, and 1.57% of dry matter, respectively. Triplic...

On Apr 1, 2011 this sequence version replaced gi:304277048.

l-Selenomethionine (SeMet) is a dominant form of selenium (Se) found in organisms at all levels of aquatic food chains and a key source of Se bioaccumulation and ecotoxicity. In mammals, intestinal absorption of SeMet is at least partly via the Na(+)-dependent neutral amino acid transporter. The mechanism of SeMet absorption and competitive effects...

A two-week trial was conducted to study the effect of feeding rates on heat shock protein levels in larval white sturgeon. The larvae (30 day post hatch, 230 mg initial body weight) were fed a commercial feed (12.6% moisture, 49.5% crude protein, 20.7% crude fat, and 8.6% ash) at 5, 15, or 25% body weight per day (BW d− 1). Liver heat shock protein...

Walleye (Sander vitreus) is an important game fish throughout much of North America. We evaluated the performance of the Wisconsin bioenergetics model for walleye in the laboratory. Walleyes were fed rainbow smelt (Osmerus mordax) in four laboratory tanks during a 126-day experiment. Based on a statistical comparison of bioenergetics model predicti...

The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Pelteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly ha...

In recent years, much progress has been made in the rearing of fish larvae fed only artificial diets. A preliminary study was made in an attempt to evaluate the effects of live food and formulated diets on survival, growth and body protein content of first-feeding larvae of Plelteobagrus fulvidraco. Three test diets varying in protein level were fo...