Christos TapeinosThe University of Manchester · Pharmacy and Optometry
Christos Tapeinos
Material Science
About
53
Publications
7,964
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1,252
Citations
Citations since 2017
Introduction
My main research interest lies in developing smart biomimetic systems that ‘read’ their microenvironment and respond to its alterations in predetermined controlled ways. Currently, I work at the University of Manchester as a Lecturer in Pharmaceutical Science specializing in Smart Nanomedicines for Brain-Targeted Drug Delivery.
Additional affiliations
May 2018 - present
Istituto Italiano di Tecnologia, Pontedera, Italy
Position
- PostDoc Position
May 2017 - April 2018
Istituto Italiano di Tecnologia, Pontedera, Italy
Position
- PostDoc Position
February 2015 - February 2017
Position
- PostDoc Position
Description
- ITN - Marie Curie Fellowship FP7 Project : AngioMatTrain
Education
May 2010 - July 2013
October 2006 - June 2009
September 2001 - September 2006
Publications
Publications (53)
Remarkable progress in phototherapy has been made in recent decades, due to its non-invasiveness and instant therapeutic efficacy. In addition, with the rapid development of nanoscience and nanotechnology, phototherapy systems based on nanoparticles or nanocomposites also evolved as an emerging hotspot in nanomedicine research, especially in cancer...
Stem cell-based therapies have shown promising results for the regeneration of the nervous system. However, the survival and integration of the stem cells in the neural circuitry is suboptimal and might compromise the therapeutic outcomes of this approach. The development of functional scaffolds capable of actively interacting with stem cells may o...
The precise control of stem cell behavior, including differentiation, transdifferentiation, and reprogramming , is fundamental for safely and efficiently using human stem cells in regenerative medicine. Thanks to recent innovations and developments in the field of nanomedicine, as well as its integration with advanced molecular biology approaches,...
Upon coming into contact with the biological environment, nanostructures are immediately covered by biomolecules, particularly by proteins forming the so-called "protein corona" (PC). The phenomenon of PC formation has gained great attention in recent years due to its implication in the use of nanostructures in biomedicine. In fact, it has been sho...
The combined approach of ex situ normothermic machine perfusion (NMP) and nanotechnology represents a strategy to mitigate ischemia/reperfusion injury in liver transplantation. We evaluated the uptake, distribution, and efficacy of antioxidant cerium oxide nanoparticles (nanoceria) during normothermic perfusion of discarded human livers. Nine disca...
Polymer capsules fabricated via the layer-by-layer (LbL) approach have attracted a great deal of attention for biomedical applications thanks to their tunable architecture. Compared to alternative methods, in which the precise control over the final properties of the systems is usually limited, the intrinsic versatility of the LbL approach allows t...
The present invention concerns a method for the production of cell-membrane derived nanoparticles, comprising the steps of extracting the cell membranes from cells of two or more different cell lines and fusing them by means of high-pressure homogenisation in the presence of a disperse therapeutic or diagnostic ingredient.
Another object of the pr...
Cardiovascular and cerebrovascular diseases (CCVDs) describe abnormal vascular system conditions affecting the brain and heart. Among these, ischemic heart disease and ischemic stroke are the leading causes of death worldwide, resulting in 16% and 11% of deaths globally. Although several therapeutic approaches are presented over the years, the cont...
Ribonucleic acid interference (RNAi) is an innovative treatment strategy for a myriad of indications. Non-viral synthetic nanoparticles (NPs) have drawn extensive attention as vectors for RNAi due to their potential advantages, including improved safety, high delivery efficiency and economic feasibility. However, the complex natural process of RNAi...
Graphene‐based materials (GBMs) demonstrate unique electrochemical, mechanical, thermal, and optical properties rendering them attractive candidates for numerous biomedical applications. Since graphene’s discovery, GBMs have been at the forefront of biomedical research offering innovative solutions for numerous diseases, including neurodegenerative...
Oxidative stress is caused by the accumulation of reactive oxygen and nitrogen species (ROS and RNS) in the cellular microenvironment. These ROS and RNS damage important cell structures leading to cell apoptosis and senescence, thus causing a detrimental effect on numerous disease pathologies such as osteoarthritis, neurodegeneration and cardiovasc...
Oxidative stress represents a common issue in most neurological diseases, causing severe impairments of neuronal cell physiological activity that ultimately lead to neuron loss of function and cellular death. In this work, lipid-coated polydopamine nanoparticles (L-PDNPs) are proposed both as antioxidant and neuroprotective agents, and as well as a...
Glioblastoma multiforme (GBM) is one of the most aggressive types of brain cancer, characterized by rapid progression, resistance to treatments, and low survival rates; the development of a targeted treatment for this disease is still today an unattained objective. Among the different strategies developed in the latest few years for the targeted de...
Cerebral ischemia (or ischemic stroke) is undeniably one of the most important life-threatening cerebral disorders. It occurs due to a clot formation in one of the blood arteries supplying the brain, causing a reduction or interruption of the blood flow. To date, the use of thrombolytics like the recombinant tissue plasminogen activator or the use...
Ischemic stroke is considered one of the most threatening neurological disorders with high percentages of mortality and morbidity worldwide. Although in recent years, several nanotechnological advances have improved the survival rates, severe untreated poststroke side‐effects continue to significantly influence the way of life of many. Tissue plasm...
Aiming at finding new solutions for fighting glioblastoma multiforme, one of most aggressive and lethal human cancer, here an in vitro validation of multifunctional nanovectors for drug delivery and hyperthermia therapy is proposed. Hybrid magnetic lipid nanoparticles have been fully characterized and tested on a multi-cellular complex model resemb...
In this study, hybrid nanocubes composed of magnetite (Fe3O4) and manganese dioxide (MnO2), coated with U‐251 MG cell‐derived membranes (CM‐NCubes) are synthesized. The CM‐NCubes demonstrate a concentration‐dependent oxygen generation (up to 15%), and, for the first time in the literature, an intracellular increase of temperature (6 °C) due to the...
Major obstacles to the successful treatment of gliolastoma multiforme are mostly related to the acquired resistance to chemotherapy drugs and, after surgery, to the cancer recurrence in correspondence of residual microscopic foci. As innovative anticancer approach, low-intensity electric stimulation represents a physical treatment able to reduce mu...
Neurodegenerative diseases comprise a large group of disorders characterized by a dramatic synaptic connections loss, occurring as a result of neurodegeneration, which is closely related to the overproduction of reactive oxygen and nitrogen species. Up to date, the treatment of neurodegenerative diseases has been limited mostly because of the inabi...
Major obstacles to the successful treatment of gliolastoma multiforme are mostly related to the acquired resistance to chemotherapy drugs and, after surgery, to the cancer recurrence in correspondence of residual microscopic foci. As innovative anticancer approach, low-intensity electric stimulation represents a physical treatment able to reduce mu...
In this study, taking into consideration the limitations of the current treatments of glioblastoma multiforme, we fabricated a biomimetic lipid-based magnetic nanovector with good loading capacity and a sustained release profile of the encapsulated chemotherapeutic drug, temozolomide. These nanostructures demonstrated an enhanced release after expo...
Osteosarcoma is an aggressive form of bone cancer mostly affecting young people. To date, the most effective strategy for the treatment of osteosarcoma is the surgical removal of the tumor with or without combinational chemotherapy. In this study, we present the development of a pH-sensitive drug-delivery system in the form of microparticles, with...
In this chapter we present the basic principles of magnetism and how these principles are altered in relation to the size of magnetic nanoparticles (MNPs), such as magnetite (Fe3O4) and maghemite (γ-Fe2O3). In addition, we make a small reference on the physical and chemical methods that are used for the synthesis of MNPs, as well as to some of the...
With the increasing advances in the fabrication and in monitoring approaches of nanotechnology devices, novel materials are being synthesized and tested for the interaction with biological environments. Among them, smart materials in particular provide versatile and dynamically tunable platforms for the investigation and manipulation of several bio...
Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) comprise a category of versatile drug delivery systems that have been used in the biomedical field for more than 25 years. SLNs and NLCs have been used for the treatment of various diseases including cardiovascular and cerebrovascular, and are considered a standard treatment...
Atherosclerosis is a chronic inflammatory disease of the arterial wall that leads to cardiovascular diseases which are the major cause of deaths worldwide. There is currently no treatment that can stop or reverse the disease. However, the use of microparticles with anti-inflammatory properties could represent a promising treatment. Herein, spherica...
Excess reactive oxygen species (ROS) has been implicated in numerous diseases including cancer, cardiovascular and neurodegenerative diseases. Overexpression of ROS can lead to oxidative stress and subsequently to H2O2-mediated cell apoptosis. In this study, it was demonstrated that biodegradable PLGA microspheres coated with collagen type I and de...
On page 5553, C. Tapeinos and A. Pandit review the properties of reactive oxygen species (ROS)-sensitive moieties which can respond to oxidative conditions inside cells. ROS-sensitive chemical structures, shown in the background of the image, are currently used in the biomedical field as drug-delivery systems, imaging probes, prochelators, and prod...
Reactive oxygen species (ROS) (H2 O2 , OCl(-) , (•) OH, O2 (-) ) are a family of reactive molecules that are generated intracellularly and are engaged in many biological processes. In physiological concentrations, ROS act as signaling molecules to a number of metabolic pathways; however, in excess they can be harmful to living organisms. Overproduc...
Resume : Introduction The uncontrollable growth of tumor cells results in decreased levels of oxygen and increased levels of reactive oxygen species (ROS) in the tumor cell microenvironment. Hence, systems that are able to scavenge ROS while increasing oxygen levels represent a promising approach to treat cancer. In the present work, poly(lactide-c...
Even though collagen hydrogels have been used in many biomedical applications due to their unique characteristics, they lacked in mechanical stability and resistance to enzymatic degradation. Aiming at the elimination of these drawbacks, collagen hydrogels have been crosslinked with a variety of synthetic crosslinkers all of which presented increas...
A soft template method is used for the synthesis of pH responsive microcontainers with inner cavity. Poly Glycidyl Methacrylate (PGMA) microspheres of narrow size distribution are synthesized by soap-free radical emulsion polymerization and the coating of the microspheres is carried out with the same procedure. The procedure consists of two steps....
Amphiphilic block copolymers are well established as building blocks for the preparation of micellar drug carriers. The functional polymer micelles possess several advantages, such as high drug efficiency, targeted delivery, and minimized cytotox-icity. The synthesis of block copolymers using nano-structured templates has emerged as a useful and ve...
Amphiphilic block copolymers are well established as building blocks for the preparation of micellar drug carriers. The functional polymer micelles possess several advantages, such as high drug efficiency, targeted delivery, and minimized cytotox-icity. The synthesis of block copolymers using nano-structured templates has emerged as a useful and ve...
Magnetic hollow spheres were synthesized through a two-step process and were evaluated by Fourier transform infrared spectroscopy, scanning electron microscopy, super quantum interference device, vibrating sample magnetometry and Mossbauer spectroscopy methods. First, polystyrene spheres (PS) were produced using emulsion polymerization. Second, the...
Projects
Projects (4)
This MSCA project aims at overcoming the limitations of the current available strategies for the treatment of post-ischemic stroke effects by introducing 'BIONICS', which represents a biomimetic and neuroprotective delivery nanocapsule. Its novelty lies to the enhanced delivery of various reactive oxygen species (ROS) and reactive nitrogen species (RNS) scavengers across the blood brain barrier (BBB), and the amelioration of the post-ischemic stroke symptoms through hypoxia and oxidative stress regulation.
The SLaMM project (ERC Starting Grant 709613) aims at the development of multifunctional lipid-based nanovectors for the delivery and the targeting of drugs to the brain, in the treatment of extremely aggressive brain tumors. The nanovectors will be tested on a specifically developed in vitro model, before their final validation in vivo.
