How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
The majority of neurons in the mammalian brain reside within the cerebellum (Cb). Yet, the evolutionary origins of the Cb are not well understood. There are several sensory nuclei present across vertebrate phylogeny collectively termed cerebellum‐like structures (CbLS) due to a shared anatomy and physiology with the Cb. Despite the similarities, th...
The developmental anatomy of the dorsal hindbrain in an elasmobranch fish, Leucoraja erinacea, is described. We focus on the cerebellum, which is a synapomorphy for gnathostomes. Cerebellar development in L. erinacea, a representative of the most basal gnathostome lineage, may be a proxy for the ancestral state of cerebellar development. We also fo...
The Inositol 1,4,5-trisphosphate receptor type 1 protein (Ip3r1) performs an essential role for the induction of cerebellar long-term depression. Here, I describe the use of RT-PCR, qPCR, western blotting and immunohistochemistry to assay Ip3r1 gene expression and localize Ip3r1 protein in the hindbrain of the elasmobranch fish, Leucoraja erinacea....
Recombinant adeno-associated viruses (AAVs) allow rapid and efficient gene delivery in the nervous system. AAVs are widely used in research and are the basis of multiple FDA-approved gene therapies. Here, we find that the immune response to AAV's genome reduces dendritic complexity in mammalian cortex. Dendritic loss associated with AAV-mediated ge...
I am doing western blots to determine if antibodies are binding selectively to the desired protein in a basal fish. I am using two different antibodies for the same protein in different vertical strips. They have different immunogens. One measures 110 kDa (the predicted molecular mass), the other measures 100 kDa. Uniprot states there is a 100 kDa isoform, but this hasn't been confirmed in our species. Is a 10 kDa difference in molecular mass acceptable considering they were run on different gels and possess different immunogens? What's an acceptable kDa range for the same protein on different gels? Thanks.