Christophe Stove

Christophe Stove
Ghent University | UGhent · Department of Bioanalysis

PharmD, PhD

About

272
Publications
48,110
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
5,463
Citations
Citations since 2016
180 Research Items
4143 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Additional affiliations
January 2003 - December 2012
Ghent University

Publications

Publications (272)
Article
Full-text available
Synthetic cannabinoids (SCs) are the largest group of compounds currently monitored in Europe by the EU Early Warning System on new psychoactive substances. Emerging recreational use of these products has led to multiple cases of adverse health effects and even death. In contrast to marijuana, where Δ9-tetrahydrocannabinol (Δ9THC) is metabolized to...
Article
Full-text available
About a century after its first described application by Ivar Bang, the potential of sampling via dried blood spots (DBS) as an alternative for classical venous blood sampling is increasingly recognized. Perhaps best known is the use of DBS in newborn screening programs, ignited by the hallmark paper by Guthrie and Susi half a century ago. However,...
Article
Full-text available
Volumetric absorptive microsampling (VAMS) is a novel sampling technique that allows the straightforward collection of an accurate volume of blood (approximately 10μL) from a drop or pool of blood by dipping an absorbent polymeric tip into it. The resulting blood microsample is dried and analyzed as a whole. The aim of this study was to evaluate th...
Article
Full-text available
Background and objective: Dried blood spot (DBS) sampling has gained wide interest in bioanalysis during the last decade and has already been successfully applied in pharmacokinetic and phenotyping studies. However, all of the available phenotyping studies used small datasets and did not include a systematic evaluation of DBS-specific parameters....
Article
A headspace-trap gas chromatography-mass spectrometry (HS-trap GC-MS) method was developed to determine GHB, a low molecular weight compound and drug of abuse, in various biological fluids. Combining this relatively novel and fully automated headspace technique with "in-vial" methylation of GHB allowed for a straightforward approach. One single met...
Article
4-Phosphoryloxy-N,N-dimethyltryptamine (psilocybin) is a naturally occurring tertiary amine found in many mushroom species. Psilocybin is a prodrug for 4-hydroxy-N,N-dimethyltryptamine (psilocin), which induces psychedelic effects via agonist activity at the serotonin (5-HT) 2A receptor (5-HT2A). Several other 4-position ring-substituted tryptamine...
Article
Dried blood spot(s) (DBS) microsampling has increasingly attracted interest as a patient-centric alternative to conventional blood withdrawal. Despite the many advantages associated with DBS sampling, its widespread use in clinical practice is still hampered, which is mainly caused by the hematocrit (Hct) effect. One approach to cope with this issu...
Article
Fully automated dried blood spot (DBS) analysis for therapeutic drug monitoring (TDM) of the immunosuppressants tacrolimus, sirolimus, everolimus and cyclosporin A suffers from a so-called hematocrit (hct) effect. This effect is related to the analysis of a partial DBS punch and extractability differences imposed by blood with different hcts. As th...
Article
Context.— The follow-up of patients under lifelong immunosuppressant therapy is pivotal to prevent allograft rejection after transplant. Part of the difficulties associated with routine monitoring of immunosuppressant concentrations can be alleviated by home sampling using dried blood spots (DBSs). Objective.— To evaluate the applicability of a DB...
Article
Full-text available
The serotonin 2A receptor (5-HT2AR) is the mediator of the psychedelic effects of serotonergic psychedelics, which have shown promising results in clinical studies for several neuropsychiatric indications. The 5-HT2AR is able to signal through the Gαq and β-arrestin effector proteins, but it is currently not known how the different signaling pathwa...
Article
Aim New psychoactive substances (NPS) are introduced on the illicit drug market at a rapid pace. They are usually poorly pharmacologically characterized, leaving users unaware of the potential harms they could be exposed to. Apart from the unknown effects and potencies of these new substances, their exact molecular targets are often inadequately el...
Article
Aim Synthetic cannabinoid receptor agonists (SCRAs) pose a danger to public health. Historically, SCRA use has been associated with a multitude of adverse events, including neurotoxicity and cardiotoxicity. Numerous case reports are published on observed SCRA toxicity, however clear studies on the toxicity of SCRAs in humans are lacking because of...
Article
Aim Synthetic cannabinoid receptor agonists (SCRAs) are a prominent danger to public health. Emerging SCRAs are most often highly active at the CB1 cannabinoid receptor. This high activity imposes serious health threats, illustrated by intoxications with SCRAs presenting at emergency departments (EDs). The rapid emergence of novel analogs makes the...
Article
Aim Controlling newly emerging drugs, such as synthetic cannabinoid receptor agonists (SCRAs), remains a never-ending challenge. They are typically abused for their psychoactive effects, related to their cannabinoid 1 (CB1) receptor activity. To evade control measures, for instance the 2021 Chinese class-wide ban, SCRAs with previously unknown stru...
Article
Aim A 25-year-old man visited his general practitioner with generalized muscle pain and severe abdominal pain including nausea, cramping and diarrhea, asking help for detoxification. The patient stated that his current complaints were the result of the use of high doses of N-piperidinyl etonitazene. After repeated intranasal administrations, he dec...
Article
Full-text available
Synthetic cannabinoid receptor agonists (SCRAs) pose a danger to public health. This study focused on individuals experiencing recreational drug toxicity who had used 5F-MDMB-PICA. Patient records were evaluated regarding vital signs, Glasgow Coma Scale (GCS) and clinical features. Liquid chromatography coupled to high-resolution mass spectrometry...
Article
Since the recent clinical approval of siRNA-based drugs and COVID-19 mRNA vaccines, the potential of RNA therapeutics for patient healthcare has become widely accepted. Lipid nanoparticles (LNPs) are currently the most advanced nanocarriers for RNA packaging and delivery. Nevertheless, the intracellular delivery efficiency of state-of-the-art LNPs...
Article
Activation of the human cannabinoid receptor type 1 (hCB1R) with high spatiotemporal control is useful to study processes involved in different pathologies related to nociception, metabolic alterations, and neurological disorders. To synthesize new agonist ligands for hCB1R, we have designed different classes of photoswitchable molecules based on a...
Article
This consensus represents the first internationally established harmonization document on PEth. It is an important document to further increase the use of PEth as a valuable alcohol biomarker in the clinical or forensic toolbox. The consensus defines the target measurand (PEth 16:0/18:1 in whole blood), cutoff concentrations (20 ng/mL and 200 ng/mL...
Article
Full-text available
The recent scheduling actions of fentanyl-related substances in both the United States and China have sparked the emergence and proliferation of other generations of “legal” opioids that are structurally distinct from fentanyl, including the recently emerged class of cinnamylpiperazines. In contrast to fentanyl, which contains a piperidine core and...
Article
Full-text available
N-Piperidinyl etonitazene (‘etonitazepipne’) represents a recent addition to the rapidly expanding class of 2-benzylbenzimidazole ‘nitazene’ opioids. Following its first identification in an online-sourced powder and in biological samples from a patient seeking help for detoxification, this report details its in-depth chemical analysis and pharmaco...
Article
Full-text available
Novel synthetic opioids continue to emerge on recreational drug markets worldwide. In response to legislative bans on fentanyl analogues, non-fentanyl structural templates, such as 2-benzylbenzimidazoles (‘nitazenes’), are being exploited to create new μ-opioid receptor (MOR) agonists. Here, we pharmacologically characterize an emerging cyclic anal...
Article
Full-text available
Objectives Fast and reliable ethanol assays analysis are used in a clinical context for patients suspected of ethanol intoxication. Mostly, automated systems using an enzymatic reaction based on ethanol dehydrogenase are used. The manuscript focusses on the evaluation of the performance of these assays. Methods Data included 30 serum samples used...
Article
Neurochemie Die Photomodulation des 5‐HT2A‐Rezeptors durch ein mit Licht kontrollierbares N,N‐Dimethyltryptaminderivat stellen Michael Decker et al. in ihrer Zuschrift vor (e202203034).
Article
Neurochemistry The photomodulation of the 5‐HT2A receptor by a light‐controllable N,N‐dimethyltryptamine derivative is reported by Michael Decker et al. in their Communication (e202203034).
Article
Full-text available
Therapeutic drug monitoring (TDM) of biologics-encompassing the measurement of (trough) concentrations and anti-drug antibodies-is emerging as a valuable tool for clinical decision making. While this strategy needs further validation, attention on its implementation into the clinic is warranted. Rapid testing and easy sampling are key to its implem...
Article
In recent years, several nations have implemented various measures to control the surge of new synthetic cannabinoid receptor agonists (SCRAs) entering the recreational drug market. In July 2021, China put into effect a new generic legislation, banning SCRAs containing one of 7 general core scaffolds. However, this has driven manufacturers towards...
Article
Following the class‐wide ban of synthetic cannabinoid receptor agonists (SCRAs) in China, SCRAs carrying new core and linker structures, aimed at circumventing the recent Chinese generic legislation, have appeared on the recreational drug market. A very recent example is (S)‐2‐(2‐(1‐(4‐fluorobenzyl)‐1H‐indol‐3‐yl)acetamido)‐3,3‐dimethylbutanamide (...
Article
Classical psychedelics are a group of hallucinogens which trigger non‐ordinary states of consciousness through activation of the 5‐HT 2A receptor (5‐HT 2A R) in the brain. However, the exact mechanism of how 5‐HT 2A R agonism alters perception remains elusive. When studying receptor signaling, tools which work at the same spatiotemporal resolution...
Article
Full-text available
Classical psychedelics are a group of hallucinogens which trigger non-ordinary states of consciousness through activation of the 5-HT 2A receptor (5-HT 2A R) in the brain. However, the exact mechanism of how 5-HT 2A R agonism alters perception remains elusive. When studying receptor signaling, tools which work at the same spatiotemporal resolution...
Article
Full-text available
The P2Y2 receptor (P2Y2R) is a G protein-coupled receptor that is activated by extracellular ATP and UTP, to a similar extent. This allows it to play roles in the cell’s response to the (increased) release of these nucleotides, e.g., in response to stress situations, including mechanical stress and oxygen deprivation. However, despite its involveme...
Article
Full-text available
Therapeutic drug monitoring (TDM) of tyrosine kinase inhibitors (TKIs) is not yet performed routinely in the standard care of oncology patients, although it offers a high potential to improve treatment outcome and minimize toxicity. TKIs are perfect candidates for TDM as they show a relatively small therapeutic window, a wide inter-patient variabil...
Article
Full-text available
Background: Synthetic cannabinoid receptor agonists (SCRAs) are amongst the largest groups of new psychoactive substances (NPS). Their often-high activity at the CB1 cannabinoid receptor frequently results in intoxications, imposing serious health risks. Hence, continuous monitoring of these compounds is important, but is challenged by the rapid e...
Article
Full-text available
New synthetic opioids (NSOs) are one of the fastest growing groups of new psychoactive substances. Amid this dynamic landscape, insight into the pharmacology of NSOs is important to estimate the harm potential of newly emerging drugs. In this work, we determined the µ-opioid receptor (MOR) affinity and activation potential of seven poorly character...
Preprint
Full-text available
INTRODUCTION The pandemic readiness toolbox needs to be extended, providing diagnostic tools that target different biomolecules, using orthogonal experimental setups and fit-for-purpose specification of detection. Here we build on a previous Cov-MS effort that used liquid chromatography-mass spectrometry (LC-MS) and describe a method that allows ac...
Article
Phosphatidylethanols (PEth) are a group of abnormal phospholipids which are formed within the human body in the presence of ethanol. As PEth accumulates in red blood cells, which prolongs its detectability, it became popular as a direct alcohol biomarker. When alcohol is consumed regularly, PEth concentrations in the human blood reach an equilibriu...
Article
New psychoactive substances (NPS), formerly also referred to as “designer drugs”, are often synthetic derivatives of existing psychoactive drugs, their diverse structures aiming at circumventing legislation and detection while their effects mimic those of traditional drugs of abuse. Of these, the group of new synthetic opioids (NSOs) has been one o...
Article
The use of alcohol, despite it being a psychoactive substance, is widely accepted. This acceptance and the ease of access may lead to alcohol abuse and dependence. Misuse of alcohol is one of the leading causes of preventable illness, injury or death, has social consequences, and can be linked to various types of criminal activities. Upon monitorin...
Article
Background and aims: Phosphatidylethanol 16:0/18:1 (PEth) is a biomarker for alcohol intake. It has a half-life of 7/8 days. Chronic alcohol consumption causes high PEth values. It can take weeks before PEth values fall below the decision limit for "alcohol abstinence". Our aim was to validate whether alcohol abstinence can be determined based on...
Article
Full-text available
Thiamin (or thiamine), known as vitamin B1, represents an indispensable component of human diets, being pivotal in energy metabolism. Thiamin research depends on adequate vitamin quantification in plant tissues. A recently developed quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) method is able to assess the level of thiamin,...
Article
Serotonergic psychedelics are substances that induce alterations in mood, perception and thought, and have the activation of serotonin (5-HT) 2A receptors (5-HT2ARs) as a main pharmacological mechanism. Besides their appearance on the (illicit) drug market, e.g. as new psychoactive substances, their potential therapeutic application is increasingly...
Data
Phenotype of all soil-grown engineered lines. All lines were grown on soil (16/8 light/dark regime; 21 °C; 50% relative humidity; 150μmol m-2 S-1 white light) and photographed at 30 days of age. Rosettes were digitally extracted to allow comparison. White bars indicate 1 cm, all images including different lines, are set to the same scale. Designati...
Article
Paracetamol (acetaminophen, APAP) is the most frequently used analgesic and antipyretic worldwide. Nonetheless, APAP induced hepatotoxicity is the most common cause of acute liver failure in the western world. This hepatotoxicity is related to the metabolism of APAP, via the formation of the electrophilic oxidation product N-acetyl-para-benzoquinon...
Article
Following our study of 4′-truncated (N)-methanocarba-adenosine derivatives that displayed unusually high mouse (m) A3AR affinity, we incorporated dopamine-related N⁶ substituents in the full agonist 5′-methylamide series. N⁶-(2-(4-Hydroxy-3-methoxy-phenyl)ethyl) derivative MRS7618 11 displayed Ki (nM) 0.563 at hA3AR (∼20,000-fold selective) and 1.5...
Article
Synthetic cannabinoid receptor agonists (SCRAs) remain one the most prevalent classes of new psychoactive substances (NPS) worldwide, and examples are generally poorly characterised at the time of first detection. We have synthesised a systematic library of amino acid-derived indole-, indazole-, and 7-azaindole-3-carboxamides related to recently...
Article
Full-text available
Background Dried blood spot (DBS) microsampling has gained interest in different clinical fields, owing to its many advantages compared to conventional blood sampling. However, whilst being applied for decades for screening purposes, some challenges, such as the hematocrit (Hct) effect, hinder further widespread use of DBS for quantitative purposes...
Article
Therapeutic drug monitoring (TDM) of tyrosine kinase inhibitors (TKIs) shows significant potential in guiding personalized anticancer treatment. Dried blood microsampling could be a valuable alternative for traditional plasma sampling to provide TDM results faster and to reach a wider audience. Sample collection is easy and patient friendly as only...
Article
Chronic treatments with dopamine D2 receptor ligands induce fluctuations in D2 receptor density. Since D2 receptors tend to assemble as homodimers, we hypothesized that receptor density might influence constitutive and ligand-induced homodimerization. Using a nanoluciferase-based complementation assay to monitor dopamine D2L receptor homodimerizati...
Article
Full-text available
Studies on the tissue distribution of the New Synthetic Opioid U‐47700 and its main metabolite N‐desmethyl‐U‐47700 revealed about 6‐fold higher metabolite concentrations in pig brain as compared to the parent compound. To better assess the toxic potential of this drug, the aim of this study was to assess the in vitro μ‐opioid receptor (MOR) activat...
Article
Full-text available
Synthetic cannabinoid receptor agonists (SCRAs) remain a prolific class of new psychoactive substances (NPS) and continue to expand rapidly. Despite the recent identification of hydroxybenzotriazole (HOBt) containing SCRAs in synthetic cannabis samples, there is currently no information regarding the pharmacological profile of these NPS with respec...
Article
Synthetic opioids constitute one of the fastest growing groups of new psychoactive substances (NPS) worldwide. With fentanyl analogues being increasingly controlled via class-wide scheduling, many non-fentanyl related opioids are now emerging on the recreational opioid market, rendering the landscape highly complex and dynamic. While new compounds...
Article
Patients receiving immunosuppressant therapy, require intensive follow-up via therapeutic drug monitoring (TDM). This puts quite a burden on the patient involving frequent hospital visits and venipunctures and could (partially) be resolved by the use of dried blood microsamples (e.g. dried blood spots, DBS). One of the drawbacks of the use of DBS i...
Article
Background: Although populations from low- and middle-income countries are at higher risk for thiamine (vitamin B-1) deficiency, accurate data on the global prevalence of thiamine deficiency are still lacking due to the difficult blood collection in remote regions. Volumetric absorptive microsampling (VAMS) from finger prick blood, generating drie...
Article
Full-text available
Biomonitoring of biological samples arises as an effective tool to evaluate the exposure to mycotoxins in the population. Owing to the wide range of advantages, there is a growing interest in the use of non- and minimally invasive alternative sampling strategies, such as dried blood spot sampling or volumetric absorptive microsampling (VAMS). A VAM...
Article
Full-text available
Thiamin (or thiamine) is a water-soluble B-vitamin (B1), which is required, in the form of thiamin pyrophosphate (TPP), as an essential cofactor in crucial carbon metabolism reactions in all forms of life. To ensure adequate metabolic functioning, humans rely on a sufficient dietary supply of thiamin. Increasing thiamin levels in plants via metabol...
Article
Full-text available
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include accurate monitoring of the spread, as this provides one of the few alternatives to total lockdown. However, COVID-...
Article
Full-text available
Some Cutibacterium acnes subgroups dominate on healthy skin while others are frequently acne-associated. Here we provide mechanistic insights into this difference, using an anaerobic keratinocyte-sebocyte-C. acnes co-culture model. An acneic C. acnes strain as well as its porphyrins activate NRLP3 inflammasome assembly, while this was not observed...
Article
Serotonergic psychedelics, substances exerting their pharmacological action through activation of the serotonin 2A receptor (5-HT2AR), have continuously comprised a substantial fraction of the over 1000 reported New Psychoactive Substances (NPS) so far. Within this category, N-benzyl derived phenethylamines, such as NBOMes and NBFs, have shown to b...
Article
Full-text available
The present work is the last of a three‐part study investigating a panel of 30 systematically designed synthetic cannabinoid receptor agonists (SCRAs) including features such as the 4‐pentenyl tail and varying head groups including amides and esters of L‐valine (MMB, AB), L‐tert‐leucine (ADB), and L‐phenylalanine (APP), as well as adamantyl (A) and...
Article
Profiling of the illicit fentanyl supply is invaluable from surveillance and intelligence perspectives. An important strategy includes the study of chemical attribution signatures (e.g. trace amounts of synthesis precursors, impurities/byproducts in seized material, metabolites in biological samples). This information provides valuable insight into...
Article
Full-text available
Förster resonant energy transfer (FRET) is a powerful mechanism to probe associations in situ. Simultaneously performing more than one FRET measurement can be challenging due to the spectral bandwidth required for the donor and acceptor fluorophores. We present an approach to distinguish overlapping FRET pairs based on the photochromism of the dono...