Christophe Rothan

Christophe Rothan
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Biologie du Fruit et Pathologie

About

189
Publications
30,548
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6,898
Citations
Additional affiliations
January 2001 - present
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Group Leader

Publications

Publications (189)
Article
Full-text available
Tomato ( Solanum lycopersicum ) is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit. In this study, we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype. By...
Article
Increased synthesis of H2O2 is observed during the initiation of fruit ripening. However, its association with plant cell processes triggering the maturation of fruit has not yet been demonstrated. The aim of this work is to investigate whether H2O2 participates in the tomato ripening process and particularly through its association with the ethyle...
Article
Full-text available
Fruit colour is central to the sensorial and nutritional quality of strawberry fruit and is therefore a major target in breeding programmes of the octoploid cultivated strawberry (Fragaria × ananassa). The red colour of the fruit is caused by the accumulation of anthocyanins, which are water-soluble flavonoids. To facilitate molecular breeding, her...
Article
Full-text available
Terrestrialization of vascular plants, i.e., Angiosperm, is associated with the development of cuticular barriers that prevent biotic and abiotic stresses and support plant growth and development. To fulfill these multiple functions, cuticles have developed a unique supramolecular and dynamic assembly of molecules and macromolecules. Plant cuticles...
Article
Full-text available
The tomato ( Solanum lycopersicum ) fruit has a thick, astomatous cuticle that has become a model for the study of cuticle formation, structure, and properties in plants. Tomato is also a major horticultural crop and a long-standing model for research in genetics, fruit development, and disease resistance. As a result, a wealth of genetic resources...
Article
Full-text available
Polyesters, as they exist in planta, are promising materials with which to begin the development of “green” replacements. Cutin and suberin, polyesters found ubiquitously in plants, are prime candidates. Samples enriched for plant polyesters, and in which their native backbones were largely preserved, were studied to identify “natural” structural f...
Article
The bZIP transcription factor (TF) SlTGA2.2 was previously highlighted as a possible hub in a network regulating fruit growth and transition to ripening (maturation phase). It belongs to a clade of TFs well known for their involvement in the regulation of the salicylic acid–dependent systemic acquired resistance. To investigate if this TGA TF plays...
Article
Full-text available
Plant architecture is central in determining crop yield. In the short‐day species strawberry, a crop vegetatively propagated via daughter‐plants produced by stolons, fruit yield is further dependent on the trade‐off between sexual reproduction (fruits) and asexual reproduction (daughter‐plants). Both are largely dependent on meristem identity, whic...
Article
Ascorbate is a major antioxidant buffer in plants, so several approaches have been developed to increase the ascorbate contents of fruits and vegetables. In this study, we combined forward genetics with mapping-by-sequencing approaches using an EMSMicro-Tom population to identify putative regulators underlying a high ascorbate phenotype in fruits....
Article
Root system architecture (RSA) manipulation may improve water and nutrient capture by plants under normal and extreme climate conditions. With the aim of initiating the genetic dissection of RSA in tomato, we established a defined ontology that allowed the curated annotation of the observed phenotypes on 12 traits at four consecutive growth stages....
Article
Full-text available
Tocochromanols constitute the different forms of vitamin E (VTE), essential for the human diet and display a high membrane protectant activity. By combining interval mapping and genome‐wide association studies (GWAS) we unveiled the genetic determinants of tocochromanol accumulation in tomato fruits. To enhance the nutritional value of this highly...
Article
Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signalling network for improving productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we i...
Article
Full-text available
The biopolyester cutin is ubiquitous in land plants, building the polymeric matrix of the plant's outermost defensive barrier, the cuticle. Cutin influences many biological processes in planta; however, due to its complexity and highly branched nature, the native structure remains partially unresolved. Our aim was to define an original workflow for...
Article
Full-text available
Main conclusion: The oxidant/antioxidant balance affects the ripening time of tomato fruit. Ripening of tomato fruit is associated with several modifications such as loss of cell wall firmness and transformation of chloroplasts to chromoplasts. Besides a peak in H2O2, reactive oxygen species (ROS) are observed at the transition stage. However, the...
Article
Cell fate maintenance is an integral part of plant cell differentiation and the production of functional cells, tissues, and organs. Fleshy fruit development is characterized by the accumulation of water and solutes in the enlarging cells of parenchymatous tissues. In tomato (Solanum lycopersicum), this process is associated with endoreduplication...
Article
Full-text available
This article comments on: Lu J, Sun J, Jiang A, Bai M, Fan C, Liu J, Ning G, Wang C. 2020. Alternate expression of CONSTANS-LIKE 4 in short days and CONSTANS in long days facilitates day-neutral response in Rosa chinensis. Journal of Experimental Botany 71, 4057–4068
Preprint
Full-text available
Fruit colour, which is central to the sensorial and nutritional quality of the fruit, is a major breeding target in cultivated strawberry (Fragaria x ananassa). Red fruit colour is caused by the accumulation of anthocyanins, which are water-soluble flavonoids. Here, using pseudo F1 progeny derived from the cv. Capitola and the advanced line CF1116...
Preprint
Full-text available
The biopolyester cutin is ubiquitous in land plants, building the polymeric matrix of the plant's outermost defensive barrier - the cuticle. Cutin influences many biological processes in planta however due to its complexity and highly branched nature, the native structure remains partially unresolved. Our aim was to define an original workflow for...
Article
Full-text available
Flavonoids are products from specialized metabolism that contribute to fruit sensorial (colour) and nutritional (antioxidant properties) quality. Here, using a pseudo full-sibling F1 progeny previously studied for fruit sensorial quality of cultivated strawberry (Fragaria x ananassa), we explored over two successive years the genetic architecture o...
Preprint
Full-text available
Flavonoids are products from specialized metabolism that contribute to fruit sensorial (colour) and nutritional (antioxidant properties) quality. Here, using a pseudo full-sibling F1 progeny previously studied for fruit sensorial quality of cultivated strawberry (Fragaria x ananassa), we explored over two successive years the genetic architecture o...
Article
Full-text available
Main conclusion Reduced GDP-l-galactose phosphorylase expression and deficiency of ascorbic acid content lead to decreased fruit set and yield in tomato plants. Abstract Reduced GDP-l-galactose phosphorylase expression and deficiency of ascorbic acid content lead to decreased fruit set and yield in tomato plants. GDP-l-galactose phosphorylase (GGP...
Conference Paper
Plants can reproduce sexually or asexually. In strawberry, both reproduction modes take place jointly and both present agronomical interests: flowering success impacts fruit yield and asexual reproduction enables cultivar propagation via stolon production. Flowering and stolon production, are physiologically and genetically linked but these traits...
Article
Flavonoids are products from specialized metabolism that contribute to fruit sensorial (colour) and nutritional (antioxidant properties) quality. Here, using a pseudo full-sibling F1 progeny previously studied for fruit sensorial quality of cultivated strawberry (Fragaria x ananassa), we explored over two successive years the genetic architecture o...
Article
The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides. The nature of the cutin‐embedded polysaccharides (CEP) and their association with cutin polyester are still unresolved. Using tomato fruit as a model, chemi...
Article
Full-text available
Strawberry is a fruit crop species of major horticultural importance, for which fruit quality and the control of flowering (for fruit yield), runnering (for vegetative propagation), and the trade-off between the two are main breeding targets. The octoploid cultivated strawberry has a limited genetic basis. This raises the question of how to identif...
Article
Full-text available
Improved plant varieties are important in our attempts to face the challenges of a growing human population and limited planet resources. Plant breeding relies on meiotic crossovers to combine favourable alleles into elite varieties¹. However, meiotic crossovers are relatively rare, typically one to three per chromosome², limiting the efficiency of...
Article
Tomato (Lycopersicum esculentum), which is used for both processing and fresh markets, is a major crop species that is ranked the first vegetable produced over the world. Tomato is also a model species for research in genetics, fruit development and disease resistances. Genetic resources available in public repositories comprise the 12 wild related...
Article
Full-text available
Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activit...
Preprint
Full-text available
Improved plant varieties are hugely significant in our attempts to face the challenges of a growing human population and limited planet resources. Plant breeding relies on meiotic crossovers to combine favorable alleles into elite varieties (1). However, meiotic crossovers are relatively rare, typically one to three per chromosome (2), limiting the...
Article
Full-text available
Light signaling has long been reported to influence fruit biology, although the regulatory impact of fruit-localized photoreceptors on fruit development and metabolism remains unclear. Studies performed in phytochrome (PHY)-deficient tomato (Solanum lycopersicum) mutants suggest that SlPHYA, SlPHYB2, and to a lesser extent SlPHYB1 influence fruit d...
Data
Heatmap showing protein levels in pericarp of fruit 20 days after anthesis (3 pools of 30 fruits) of the transgenic lines and wild-type. All proteins were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. The log2 of the protein ratio with the wild-type for each transgenic line is presented. The scale goes from g...
Data
Gene expression profiles for AO, Solyc04g054690; GLD, Solyc10g079470 and MDHAR, Solyc09g009390 as obtained from the TomExpress tool (http://tomexpress.toulouse.inra.fr).
Data
Fruit metabolite data (orange fruit pericarp). Quantitative data for the following metabolites: galactose, mannose, glutamate, glutamine, alanine, aspartate, tyrosine, fructose, glucose, sucrose, ascorbate, dehydroascorbate, GABA, citramalate, citrate, malate, chlorogenic acid, caffeic acid glucoside, cis-chlorogenic acid, quercetin derivative, and...
Data
WGCNA module identification. A graphical representation of the data from Supplementary Table S4: module eigengenes are labeled by color and the module membership scale from red (high adjacency, positive correlation) to green (low adjacency, negative correlation) shown for each metabolite and protein. The 7633 genes were used to generate the topolog...
Data
Proteins. Raw data for quantifiable proteins (see Materials and methods) from orange pericarp with spot identifier code for each of the three pools for the wild type, AO, GLD, and MDHAR RNAi lines.
Data
Gene expression profiles for AO, Solyc04g054690; GLD, Solyc10g079470 and MDHAR, Solyc09g009390 as obtained from the Solgenomics expression atlas tool (http://tea.solgenomics.net).
Data
Ascorbate and dehydroascorbate levels in pericarp fruit 20 days after anthesis of transgenic lines and WT. Ascorbate and dehydroascorbate were assayed in the pericarp tissue (3 biological replicates of 30 fruits per pool) of the lines by a spectrophotometric method. Measurements show means with standard error (SE). A comparison of means was carried...
Data
WGCNA. Solyc identifier, gene function, GO terms, module color, module membership (MM or eigengene-based connectivity) and associated p-value for the 38 modules and 7633 genes as generated by the WGCNA R package.
Data
Transcriptome raw data. For each of the 7633 genes mean expression (normalized) in each of the three transgenic lines and wild type with standard error (SE). F values, p-values, false discovery rate corrected p-values and fold change (FC) are also given for each comparison as follows: 1: AO vs. WT, 2: GLD vs. WT, 3: MDHAR vs. WT, 4: AO vs. MDHAR, 5...
Article
Full-text available
Changing the balance between ascorbate, monodehydroascorbate, and dehydroascorbate in plant cells by manipulating the activity of enzymes involved in ascorbate synthesis or recycling of oxidized and reduced forms leads to multiple phenotypes. A systems biology approach including network analysis of the transcriptome, proteome and metabolites of RNA...
Poster
Strawberry, the major berry fruit crop, is a relevant model to study the occurrence and concomitance of various developmental processes/events. Vegetative reproduction through stolons (elongated stems) and sexual reproduction through inflorescences occur successively or jointly, depending on the genotype, cultural technique and environment. The con...
Article
Improving crop productivity and quality while promoting sustainable agriculture have become major goals in plant breeding. The cuticle is a natural film covering the aerial organs of plants and consists of lipid polyesters covered and embedded with wax. The cuticle protects plants against water loss and pathogens and affects traits with strong impa...
Article
Full-text available
Asexual and sexual reproduction occur jointly in many angiosperms. Stolons (elongated stems) are used for asexual reproduction in the crop species potato (Solanum tuberosum) and strawberry (Fragaria spp.), where they produce tubers and clonal plants, respectively. In strawberry, stolon production is essential for vegetative propagation at the expen...
Poster
Tissue morphogenesis and overall fruit growth depend on complex cellular and molecular interactions that affect the balance between cell division and cell expansion. The molecular control of fruit tissue morphogenesis and growth remain largely underexplored and only few regulators have been already identified. Forward genetics appears as the most p...
Chapter
Tomato is currently the model species for fleshy fruit development and for Solanaceae species. The recent completion of a high-quality genome sequence of the inbred tomato (Solanum lycopersicum) cultivar ‘Heinz 1706’ allowed the prediction and in silico annotation of ca 35,000 genes. Assigning a biological function to these genes is among the prior...
Article
The tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutat...
Data
Figure S1 Position on the F. vesca subsp. bracteata pseudochromosome 4 (Tennessen et al., 2014) of the markers linked to perpetual flowering in the octoploid strawberry developed in this study (in bold), in Castro et al. (2015) and in Honjo et al. (2016) (in italic).
Data
Table S1 Description of the 15 and 11 female and male bins of the four linkage groups (LGs) IV in the octoploid Fragaria reference map. Table S2 Number of recombinants according to their population of origin, genotype and phenotype. Individuals were fine mapped between Bx089_196 and Bx064_216 SSRs. Phenotype was recorded as Perpetual Flowering (PF...
Article
Full-text available
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of th...
Article
Full-text available
Extending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry (Fragaria x ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting perpetual flowering (PF) cultivars. This trait is of considerable inte...
Article
Full-text available
Abstract The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micr...
Article
Full-text available
Tocopherol, a compound with vitamin E (VTE) activity, is a conserved constituent of the plastidial antioxidant network in photosynthetic organisms. The synthesis of tocopherol involves the condensation of an aromatic head group with an isoprenoid prenyl side chain. The latter, phytyl diphosphate, can be derived from chlorophyll phytol tail recyclin...
Book
Micro-Tom tomato cultivar is particularly adapted to the development of genomic approaches in tomato. Here, we describe the culture of this plant in greenhouse, including climate regulation, seed sowing and watering, vegetative development, plant maintenance, including treatment of phytosanitary problems, and reproductive development.
Article
Cuticle function is closely related to the structure of the cutin polymer. However, the structure and formation of this hydrophobic polyester of glycerol and hydroxy/epoxy fatty acids has not been fully resolved. An apoplastic GDSL-lipase kown as cutin synthase 1 (CUS1) is required for cutin deposition in tomato fruit exocarp. In vitro, CUS1 cataly...
Article
Micro-Tom tomato cultivar is particularly adapted to the development of genomic approaches in tomato. Here, we describe the culture of this plant in greenhouse, including climate regulation, seed sowing and watering, vegetative development, plant maintenance, including treatment of phytosanitary problems, and reproductive development.
Article
Xanthophylls, the pigments responsible for yellow to red coloration, are naturally occurring carotenoid compounds in many colored tissues of plants. These pigments are esterified within the chromoplast; however, little is known about the mechanisms underlying their accumulation in flower organs. In this study, we characterized two allelic tomato (S...