Christophe Hirtz

Christophe Hirtz
Université de Montpellier | UM1 · Clinical Proteomics Platform/Laboratory of Biochemistry and Clinical Proteomics

Professor

About

192
Publications
34,326
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Introduction
Christophe Hirtz was born in 1972 and studied Biochemistry at Paul Sabatier University in Toulouse (France). Research Technician during 12 years, he got his Doctorate (2005) and an accreditation to supervise research (2010) in the clinical proteomic field. He was recruited in 2008 as Associate Professor at the University of Montpellier (France) in Biochemistry and Clinical Proteomics. His scientific interest includes the development of new methods of protein quantification using targeted mass spectrometry in a clinical environment. He is in responsible of the Clinical Proteomic Platform of Montpellier.The University of Montpellier recognize his scientific excellence through regular evaluation. 70 international articles (H factor:18), 24 international and 16 national oral communications.
Additional affiliations
January 2012 - present
January 2011 - November 2017
Université de Montpellier
Position
  • Professor (Full)
January 2010 - January 2015
Université de Montpellier
Position
  • Professor (Associate)

Publications

Publications (192)
Article
Background: Little is known about the influence of Riluzole on serum neurofilament light chain (sNfL) levels, a biomarker of prognosis in amyotrophic lateral sclerosis (ALS), and variations with time of sNfL concentrations are controversial. Methods: Sera from ALS patients (n = 141) and controls (n = 33) were collected at inclusion (sNfL1) and s...
Article
Full-text available
Introduction: We explored what combination of blood-based biomarkers (amyloid beta [Aβ]1-42/1-40, phosphorylated tau [p-tau]181, neurofilament light [NfL], glial fibrillary acidic protein [GFAP]) differentiates Alzheimer's disease (AD) dementia, frontotemporal dementia (FTD), and dementia with Lewy bodies (DLB). Methods: We measured the biomarke...
Preprint
Neurofilament light chain (Nf-L) is a well-known biomarker for axonal damage, however the corresponding circulating Nf-L analyte in CSF is poorly characterized. We, therefore, isolated new monoclonal antibodies against synthetic peptides, and these monoclonals were characterized for their specificity on brain-specific intermediate filament proteins...
Article
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Parkinson’s disease is a complex neurodegenerative disorder resulting in a multifaceted clinical presentation which includes bradykinesia combined with either rest tremor, rigidity, or both, as well as many non-motor symptoms. The motor features of the disorder are associated with the pathological form of alpha synuclein aggregates and fibrils in L...
Article
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The present study focuses on the use of a metaproteomic approach to analyze Black Extrinsic Tooth Stains, a specific type of pigmented extrinsic substance. Metaproteomics is a powerful emerging technology that successfully enabled human protein and bacterial identification of this specific dental biofilm using high-resolution tandem mass spectromet...
Article
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Introduction Blood biomarkers represent a major advance for improving the management, diagnosis, and monitoring of Alzheimer's disease (AD). However, their context of use in relation to routine cerebrospinal fluid (CSF) analysis for the quantification of amyloid peptides and tau proteins remains to be determined. Methods We studied in two independ...
Article
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Voluntary asphyxia imposed by static apnea challenges blood-brain barrier (BBB) integrity in humans through transient extremes of hypertension, hypoxemia and hypercapnia. In the present study, ten ultra-elite breath-hold divers performed two maximal dry apneas preceded by normoxic normoventilation (NX: severe hypoxemia and hypercapnia) and hyperoxi...
Preprint
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Menstrual cups are gaining in popularity worldwide as a more environmentally sustainable and affordable alternative to disposable personal hygiene products. However, there is currently a lack of scientific literature regarding their safety and potential effects on women’s health. We analysed biological, demographic, and behavioural data in a cohort...
Article
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Recurrent contact and concussion in rugby union remains a significant public health concern given the potential increased risk of neurodegeneration in later-life. This study determined to what extent prior-recurrent contact impacts molecular-haemodynamic biomarkers underpinning cognition in current professional rugby union players with a history of...
Article
To ensure reliable diagnosis and treatment consistently throughout healthcare, metrological quality assurance is essential. There are, however, many observations in the social sciences and healthcare, such as the memory tests studied here, which have been ‘claimed’ to be measurements but in fact have not been fully metrologically legitimated. We ha...
Article
Monoclonal antibodies (mAbs) are large size molecules that have demonstrated high therapeutic potential for the treatment of cancer or autoimmune diseases. Despite some excellent results, their intravenous administration results in high plasma concentration. This triggers off-target effects and sometimes poor targeted tissue distribution. To circum...
Article
Pre‐analytical sample handling might affect the results of Alzheimer’s blood‐based biomarkers. In this collaborative study of the SABB‐GBSC working group guided by the Alzheimer’s Association, we elucidate the effects of the most common sample handling variations on blood‐based amyloid and non‐amyloid biomarkers. Freshly collected blood was differe...
Article
Neurofilament‐light chain (NF‐L) is a known early marker for neurodegeneration. This marker which is essential for the axonal structure, is quantifiable in biological fluids after neuronal damage but is not specific to a given pathology. Currently, the methods for detecting/quantifying this biomarker are mainly based on immunoassays. While the perf...
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Full-text available
Background The diagnosis of skin wound vitality is currently based on standard histology, but histological findings lack sensitivity in case of a short survival time. New reliable biomarkers of vitality are therefore strongly needed. We assessed the ability of 10 candidate cytokines (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF...
Article
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Introduction: Pre-analytical sample handling might affect the results of Alzheimer's disease blood-based biomarkers. We empirically tested variations of common blood collection and handling procedures. Methods: We created sample sets that address the effect of blood collection tube type, and of ethylene diamine tetraacetic acid plasma delayed ce...
Article
Despite significant progress in targeted therapies, cancer recurrence remains a major cause of mortality worldwide. Identification of accurate biomarkers, through molecular profiling in healthy and cancer patient samples, will improve diagnosis and promote personalized medicine. While genetic and epigenetic alterations of DNA are currently exploite...
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Background Tau proteins are recognized biomarkers of neurodegeneration and neuronal damage in the cerebrospinal fluid (CSF). It has also been suggested that these CSF proteins could increase post-mortem due to neuronal death. The aim of this study was to investigate the changes in CSF total and phosphorylated tau (p-tau) levels in the early post-mo...
Article
In patients with metastatic breast cancer (MBC), brain metastases (BM) are associated with high morbidity and mortality. However, there is no validated serum biomarker that accurately predicts BM occurrence in these patients, and the role of serum biomarkers for prognosis remains unclear. Here, we evaluated the association of Neurofilament light ch...
Article
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Objectives All categories included in the AT(N) classification can now be measured in plasma. However, their agreement with cerebrospinal fluid (CSF) markers is not fully established. A blood signature to generate the AT(N) classification would facilitate early diagnosis of patients with Alzheimer’s disease (AD) through an easy and minimally invasi...
Article
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Aim: To compare methods of quantifying serum hepcidin (based on MS and ELISA) and their ability to diagnose true iron deficiency anemia in critically ill patients. Materials & methods: Serum hepcidin was measured in 119 critically ill patients included in the HEPCIDANE clinical trial, using either an ultra-sensitive ELISA kit (from DRG) or two diff...
Article
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Human papillomaviruses (HPVs) are oncogenic viruses causing most cervical cancers. Highly prevalent in young, sexually active women, only a minority of HPV infections persist. To better characterize the immuno-modulatory impact of early HPV infections, we measured changes in a panel of 20 cytokines in cervicovaginal samples collected from young wom...
Preprint
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Forced and maintained expression of four transcription factors OCT4, SOX2, KLF4 and c-MYC (OSKM), can reprogram somatic cells into induced Pluripotent Stem Cells (iPSCs) and a limited OSKM induction is able to rejuvenate the cell physiology without changing the cell identity. We therefore sought to determine if a burst of OSKM might improve tissue...
Chapter
Quantification of tau proteins in the cerebrospinal fluid is a major biomarker for the positive diagnosis of Alzheimer’s disease, as well as for other neurodegenerative diseases and brain damage monitoring. Tau is present in this fluid in multiple proteoforms, different by their lengths and phosphorylation sites. Immunodetection approaches for “tot...
Article
Periodontitis is a complex immune-inflammatory condition characterized by the disruption of the periodontal ligament and subsequent formation of periodontal pockets, and by alveolar bone loss, often resulting in tooth loss. A myriad of factors, namely, genetic, metabolic, immunological, and inflammatory, is associated with progression of periodonti...
Article
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Cancer stem cells (CSCs) are a small but critical cell population for cancer biology since they display inherent resistance to standard therapies and give rise to metastases. Despite accruing evidence establishing a link between deregulation of epitranscriptome-related players and tumorigenic process, the role of messenger RNA (mRNA) modifications...
Article
Full-text available
This study aimed to evaluate the changes in the activity of total salivary alpha-amylase (TsAA) and both the non-glycosylated and glycosylated salivary alpha-amylase proteoforms (NGsAA and GsAA, respectively) in physical and psychological stress models, estimated using a simple and easily set-up method. The method used was a spectrophotometric assa...
Chapter
The human body has many different hormones that interact with each other and with other factors such as proteins, cell receptors and metabolites. There is still a limited understanding of some of the underlying biological mechanisms of some hormones. In the past decades, science and technology have made major advancements in regard to innovation an...
Preprint
Full-text available
Human papillomaviruses (HPVs) are the most oncogenic viruses known to human, causing nearly all cervical cancers worldwide. Highly prevalent in young, sexually active women, most HPV infections are cleared within 3 years, and only a minority of those infections persist and lead to cancer later in life. To better characterize the immuno-modulatory i...
Article
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Within the neurovascular unit (NVU), the blood-brain barrier (BBB) operates as a key cerebrovascular interface, dynamically insulating the brain parenchyma from peripheral blood and compartments. Increased BBB permeability is clinically relevant for at least two reasons: it actively participates in the etiology of central nervous system (CNS) disea...
Article
Early and accurate diagnosis of Alzheimer’s disease relies on different techniques, including cognitive tests, imaging and biomarkers measurement. Here we describe the potential of the use of a well characterized primary calibrator (traceable to the SI) and the development of a candidate reference method by LC‐IDMS for worldwide standardization of...
Article
The use of biomarkers which reflect Alzheimer’s disease (AD) improved the stratification of patients and the ability to propose personalized care. Their detection using either cerebrospinal fluid analysis (CSF), which is invasive, or using PET imaging, which is costly, is not adapted to large‐scale use. In 2018, Nakamura et al. using MALDI‐TOF IP‐M...
Article
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The use of dental stem cells has raised many hopes in the development of new treatments for neurodegenerative diseases. According to current statistics, about 1 in 6 people in the world would be affected by a neurological disease. This number continues to increase as the world's population ages, making neurodegenerative diseases probably the one of...
Article
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Background Amyloid pathology, which is one of the characteristics of Alzheimer’s disease (AD), results from altered metabolism of the beta-amyloid (Aβ) peptide in terms of synthesis, clearance, or aggregation. A decrease in cerebrospinal fluid (CSF) level Aβ1–42 is evident in AD, and the CSF ratio Aβ42/Aβ40 has recently been identified as one of th...
Article
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The association between restless legs syndrome (RLS) and iron homeostasis remains unclear. We compared serum hepcidin and ferritin levels in patients with RLS and controls, and assessed their relationships with RLS phenotype, drug intake, and history of augmentation syndrome. 102 drug-free RLS patients (age 58.9 [24.5–77.2], 63 females) and 73 cont...
Article
Full-text available
Neurofilament light chain (NfL) has been demonstrated to correlate with multiple sclerosis disease severity as well as treatment response. Nevertheless, additional serum biomarkers are still needed to better differentiate disease activity from disease progression. The aim of our study was to assess serum glial fibrillary acid protein (s-GFAP) and n...
Preprint
Full-text available
Background: Amyloid pathology, which is one of the characteristics of Alzheimer's disease (AD), results from altered metabolism of the beta-amyloid peptide (Aβ) in terms of synthesis, clearance or aggregation. A decrease in cerebrospinal fluid (CSF) level Aβ1-42 is evident in AD, and the CSF ratio Aβ40 /Aβ40 has recently been identified as one of t...
Article
Full-text available
The present study examined if repeated bouts of micro- and hypergravity during parabolic flight (PF) alter structural integrity of the neurovascular unit (NVU) subsequent to free radical-mediated changes in regional cerebral perfusion. Six participants (5♂, 1♀) aged 29 ± 11 years were examined before, during and after a 3h PF and compared to six se...
Article
The performances of three commercial albumin extraction methods for the isolation of intact albumin-amyloid beta peptide (HSA-Aβ) complexes from serum were compared using different analytical approaches. To determine the extraction yield, the repeatability and the selectivity of the extraction procedures, a capillary electrophoresis coupled to UV d...
Article
Full-text available
Background: Cerebrospinal fluid biomarker profiles characterized by decreased amyloid-beta peptide levels and increased total and phosphorylated tau levels at threonine 181 (pT181) are currently used to discriminate between Alzheimer's disease and other neurodegenerative diseases. However, these changes are not entirely specific to Alzheimer's dis...
Article
Full-text available
Background: Memory troubles and hippocampal atrophy are considered more frequent and focal atrophy less severe in late-onset (>65 years) than in presenile behavioral variant frontotemporal dementia (bvFTD). Objective: To compare cerebrospinal fluid (CSF) and plasma biomarkers in late-onset and presenile bvFTD. Methods: Multicentric retrospecti...
Preprint
Full-text available
Cancer stem cells (CSCs) are a small but critical cell population for cancer biology since they display inherent resistance to standard therapies and give rise to metastases. Despite accruing evidence establishing a link between deregulation of epitranscriptome-related players and tumorigenic process, the role of messenger RNA (mRNA) modifications...
Article
Full-text available
Background . Nowadays, invasive fungal infections (IFI) are of increasing importance and associated with an increased mortality. However, reliable diagnostic tools for the identification of patients suffering from an IFI are rare and associated with relevant weaknesses. Methods . Within this secondary analysis of an observational clinical study, an...
Article
Full-text available
Objectives To evaluate the sAA proteoforms’ expression during different stimulation situations. Materials and methods This study evaluated the salivary alpha-amylase (sAA) proteoforms’ behavior by western blot (WB) analysis and high-resolution mass spectrometry (LC-MS/MS) in different situations that produce increases in sAA activity. For this pur...
Article
Full-text available
The extraction of accurate physiological parameters from clinical samples provides a unique perspective to understand disease etiology and evolution, including under therapy. We introduce a new methodologic framework to map patient proteome dynamics in vivo, either proteome-wide or in large targeted panels. We applied it to ventricular cerebrospina...
Article
Full-text available
Alzheimer’s disease (AD) is an incurable neurodegenerative disease characterized by progressive decline of cognitive abilities. Amyloid beta peptides (Aβ), Tau proteins and the phosphorylated form of the Tau protein, p-Tau, are the core pathological biomarkers of the disease, and their detection for the diagnosis of patients is progressively being...
Preprint
Full-text available
The extraction of accurate physiological parameters from clinical samples provides a unique perspective to understand disease etiology and evolution, including under therapy. We introduce a new proteomics framework to map patient proteome dynamics in vivo, either proteome wide or in large targeted panels. We applied it to ventricular cerebrospinal...
Article
Full-text available
Alzheimer disease (AD) is one of several neurodegenerative diseases characterized by dysregulation, misfolding and accumulation of specific proteins in the CNS. The stable isotope labelling kinetics (SILK) technique is based on generating amino acids labelled with naturally occurring stable (that is, nonradioactive) isotopes of carbon and/or nitrog...
Article
Full-text available
Introduction Human papillomaviruses (HPVs) are responsible for one-third of all cancers caused by infections. Most HPV studies focus on chronic infections and cancers, and we know little about the early stages of the infection. Our main objective is to better understand the course and natural history of cervical HPV infections in healthy, unvaccin...
Poster
Full-text available
Multiplex quantification of RNA methylation by targeted mass spectrometry
Article
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Global, segmental, and gene duplication–related processes are driving genome size and complexity in plants. Despite their evolutionary potentials, those processes can also have adverse effects on genome regulation, thus implying the existence of specialized corrective mechanisms. Here, we report that an N6-methyladenosine (m ⁶ A)–assisted polyadeny...
Article
Full-text available
The accurate estimation of the time of death is a challenge in forensic medicine, as the methods routinely used to assess the post-mortem interval (PMI) are far from being precise. Over the past decades, biochemical methods have been implemented on post-mortem samples to improve the precision of PMI estimation. Studies have focussed on the biochemi...
Article
Full-text available
One of the main hallmarks of neurodegenerative diseases is the loss of neurons in the brain and/or spinal cord. The clinical characteristics of neurodegenerative diseases depend on the specific regions of the central nervous system that have undergone cell loss. These diseases place an enormous burden on society due to the degree of human suffering...
Article
Full-text available
While the dose-response relationship of radiation-induced bystander effect (RIBE) is controversial at low and high linear energy transfer (LET), mechanisms and effectors of cell-to-cell communication stay unclear and highly dependent of cell type. In the present study, we investigated the capacity of chondrocytes in responding to bystander factors...
Conference Paper
Objectif : Évaluer les taux d’hepcidine et de ferritine dans le sérum de patients présentant un syndrome des jambes sans repos (SJSR) en condition traitée et non-traitée en comparaison à des témoins. Méthodes: Au total, 102 patients avec un SJSR non-traité (63 femmes, âge médian 58,91 ans, 15 avec ferritine < 50 μg/l) dont 17 présentaient un syn...