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Christophe Grosset

Christophe Grosset
Bordeaux Institute of Oncology · U1312 Inserm

PhD

About

97
Publications
6,348
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Introduction
“Our team uses different in vitro and in vivo experimental approaches to study biological phenomena driving tumorigenesis. Its projects focus on the role of signaling pathways, genes and microRNAs in different types of adult and pediatric cancers with bad prognosis. The team has validated its competences by patenting novel therapeutic molecules and establishing novel tumor models in mice and chick embryo for drug screening”. Are you looking for research opportunities on pediatric cancers? Yes? See our project 2. ORCID: https://orcid.org/0000-0002-0479-6291
Additional affiliations
January 2016 - December 2020
French National Institute of Health and Medical Research
Position
  • Research Director
December 2015 - December 2020
French National Institute of Health and Medical Research
Position
  • Research Director
January 1999 - December 2013
Université Victor Segalen Bordeaux 2
Education
December 2015 - January 2020
French Institute of Health and Medical Research
Field of study
  • Biology and Health, liver cancer in adults and children
November 2001
October 1995 - January 1998
University BordeauxSegalen
Field of study
  • Biology and Health

Publications

Publications (97)
Article
Full-text available
Angiogenesis involves cell specification orchestrated by regulatory interactions between the vascular endothelial growth factor and Notch signaling pathways. However, the role of microRNAs in these regulations remains poorly explored. Here we show that a controlled level of miR-155 is essential for proper angiogenesis. In the mouse retina angiogene...
Article
Full-text available
Background Diffuse Midline Glioma, H3K27M-mutant (DMG) is a rare, highly aggressive pediatric tumor affecting the brainstem, and is one of the deadliest cancers. Currently available treatment options such as chemotherapy and radiotherapy do only modestly prolong survival. In this pathology, H3K27 mutations deregulate Polycomb Repressive Complex 2 (...
Article
Full-text available
Introduction: Hepatocellular carcinoma and hepatoblastoma are two liver cancers characterized by gene deregulations, chromosomal rearrangements, and mutations in Wnt/beta-catenin (Wnt) pathway-related genes. LHX2, a transcriptional factor member of the LIM homeobox gene family, has important functions in embryogenesis and liver development. LHX2 is...
Article
Full-text available
Despite recent progress in the characterization of tumour components, the tri-dimensional (3D) organization of this pathological tissue and the parameters determining its internal architecture remain elusive. Here, we analysed the spatial organization of patient-derived xenograft tissues generated from hepatoblastoma, the most frequent childhood li...
Article
Full-text available
Mitogen-activated protein kinases (MAPKs) are essential for proper cell functioning as they regulate many molecular effectors. Careful regulation of MAPKs is therefore required to avoid MAPK pathway dysfunctions and pathologies. The mammalian genome encodes about 200 phosphatases, many of which dephosphorylate the MAPKs and bring them back to an in...
Preprint
Full-text available
Despite recent progress in the characterization of tumour components, the tri-dimensional (3D) organization of this pathological tissue and the parameters determining its internal architecture remain elusive. Here, we analysed the spatial organization of patient-derived xenograft tissues generated from hepatoblastoma, the most frequent childhood li...
Preprint
Full-text available
BACKGROUND & AIMS ß-catenin is a well-known effector of the Wnt pathway and a key player in cadherin-mediated cell adhesion. Oncogenic mutations of ß-catenin are highly frequent in pediatric liver primary tumors. Those mutations are mostly heterozygous allowing the co-expression of wild-type (WT) and mutated ß-catenins in tumor cells. We investigat...
Article
Full-text available
Résumé Depuis leur découverte dans les années 1990, les microARN (miARN) fascinent les scientifiques par leur capacité à réguler post-transcriptionnellement et de façon fine un large spectre de gènes et à contrôler la quasi-totalité des processus cellulaires. Les miARNs sont des médiateurs clés de la reprogrammation génique et des fonctions cellula...
Article
Full-text available
Enterohepatic Helicobacters, such as Helicobacter hepaticus and Helicobacter pullorum, are associated with several intestinal and hepatic diseases. Their main virulence factor is the cytolethal distending toxin (CDT). In the present study, whole genome microarray-based identification of differentially expressed genes was performed in vitro in HT-29...
Article
Full-text available
Since their discovery in the mid-1990s, microRNAs (miRNAs) have captured the attention of the scientific community by their ability to post-transcriptionally and subtly regulate a large but still poorly defined set of gene targets and to control most cellular and molecular processes, making them central effectors of gene reprogramming and cell func...
Article
Background: Diffuse midline glioma (DMG) is a pediatric malignancy with a poor prognosis. Most children die less than one year after diagnosis. Recently, specific mutations in histone H3 have been identified and are believed to be oncogenic drivers. Targeting this epigenetic abnormality using HDAC inhibitors such as panobinostat (PS) is therefore a...
Article
Background: Therapeutic outcomes using the multikinase inhibitors sorafenib or regorafenib remain unsatisfactory for patients with advanced hepatocellular carcinoma (HCC). Thus, new drug modalities are needed. We recently reported the remarkable capacity of miR-4510 to impede the growth of HCC and hepatoblastoma through Glypican-3 (GPC3) targeting...
Article
Full-text available
Humans are frequently exposed to bacterial genotoxins involved in digestive cancers, colibactin and Cytolethal Distending Toxin (CDT), the latter being secreted by many pathogenic bacteria. Our aim was to evaluate the effects induced by these genotoxins on nuclear remodeling in the context of cell survival. Helicobacter infected mice, coculture exp...
Article
Full-text available
Les microARN (miARN) sont des petits ARN non-codants de 18 à 24 nucléotides présents chez les animaux, les végétaux et certains virus. Leur principale fonction biologique est de réguler négativement et de façon fine, dynamique et temporelle l’expression des gènes, en contrôlant étroitement le taux de protéines produites. Ils jouent donc un rôle ess...
Conference Paper
Identification of new treatments for relapsing pediatric cancer is an unmet clinical need and a societal challenge. Hepatoblastoma (HB) is a rare tumor that constitutes the most frequent form of childhood liver cancer. Current improvement in medical care allows 80% of children to survive this disease, but no second line treatment is available at re...
Conference Paper
Identification of new treatments for relapsing pediatric cancer is an unmet clinical need and a societal challenge. Hepatoblastoma (HB) is a rare tumor that constitutes the most frequent form of childhood liver cancer. Current improvement in medical care allows 80% of children to survive this disease, but no second line treatment is available at re...
Presentation
Full-text available
Flyer exposing the objectives of our work on pédiatric cancers
Article
Full-text available
A major limitation in the development of efficient clinical protocols for mesenchymal stromal cell (MStroC)-based tissue regeneration therapy is the low retention and survival of MStroC in injured tissue after therapeutic administration. Low oxygen concentration preconditioning (LOP) during ex vivo cultivation of MStroC, as a method for mimicking o...
Article
Full-text available
Hepatoblastoma (HBL) is a pediatric liver cancer with defined molecular alterations driving its progression. Here, we describe an animal model for HBL on the chick chorioallantoic membrane (CAM), which recapitulates relevant features of HBL in patients. Expression of classic tumor-associated proteins such as β-catenin, EpCAM and CK19 was maintained...
Article
Glypican 3 (GPC3) is a complex heparan sulfate proteoglycan associated with the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor (GPI). It is also N-glycosylated and processed by a furine-like convertase. GPC3 has numerous biological functions. While undetectable in normal liver tissue, it is abnormally and highly over...
Article
Full-text available
Surgery and cisplatin-based treatment of hepatoblastoma (HB) currently guarantee the survival of 70-80% of patients. However, some important challenges remain in diagnosing high risk tumors and identifying relevant targetable pathways offering new therapeutic avenues. Previously, two molecular subclasses of hepatoblastoma tumors have been described...
Article
Full-text available
Background The evolution of next-generation sequencing (NGS) technologies has led to increased focus on RNA-Seq. Many bioinformatic tools have been developed for RNA-Seq analysis, each with unique performance characteristics and configuration parameters. Users face an increasingly complex task in understanding which bioinformatic tools are best for...
Article
Full-text available
Glypican-3 (GPC3) is an oncogene, frequently upregulated in liver malignancies such as hepatocellular carcinoma (HCC) and hepatoblastoma and constitutes a potential molecular target for therapy in liver cancer. Using a functional screening system, we identified 10 new microRNAs controlling GPC3 expression in malignant liver cells, five of them e.g....
Article
Full-text available
Hepatoblastoma (HBL) is the most common pediatric liver cancer. In this malignant neoplasm, beta-catenin protein accumulates and increases Wnt signaling due to recurrent activating mutations in the CTNNB1 gene. Therefore, beta-catenin is a key therapeutic target in HBL. However, controlling beta-catenin production with therapeutic molecules has bee...
Article
Full-text available
MicroRNAs (miRNA) are generally described as negative regulators of gene expression. However, some evidence suggests that they may also play positive roles. As such, we reported that miR-1291 leads to a GPC3 mRNA expression increase in hepatoma cells through a 3' untranslated region (UTR)-dependent mechanism. In the absence of any direct interactio...
Article
Full-text available
Unlabelled: Hepatocellular carcinoma (HCC) is the major primary liver cancer. Glypican-3 (GPC3), one of the most abnormally expressed genes in HCC, participates in liver carcinogenesis. Based on data showing that GPC3 expression is posttranscriptionally altered in HCC cells compared to primary hepatocytes, we investigated the implication of microR...
Article
Full-text available
Besides the fact that miR-96 and miR-182 belong to the miR-182/183 cluster, their seed region (UUGGCA, nucleotides 2–7) is identical suggesting potential common properties in mRNA target recognition and cellular functions. Here, we used the mRNA encoding Glypican-3, a heparan-sulfate proteoglycan, as a model target as its short 3′ untranslated regi...
Article
Full-text available
An increasing number of arguments, including altered microRNA expression, support the idea that post-transcriptional deregulation participates in gene disturbances found in diseased tissues. To evaluate this hypothesis, we developed a method which facilitates post-transcriptional investigations in a wide range of human cells and experimental condit...
Article
Full-text available
A family with dominant X-linked chondrodysplasia was previously described. The disease locus was ascribed to a 24 Mb interval in Xp11.3–q13.1. We have identified a variant (c.∗281A>T) in the 3′ untranslated region (UTR) of the HDAC6 gene that totally segregates with the disease. The variant is located in the seed sequence of hsa-miR-433. Our data s...
Article
Full-text available
A family with dominant X-linked chondrodysplasia was previously described. The disease locus was ascribed to a 24 Mb interval in Xp11.3-q13.1. We have identified a variant (c.*281A>T) in the 3′ untranslated region (UTR) of the HDAC6 gene that totally segregates with the disease. The variant is located in the seed sequence of hsa-miR-433. Our data s...