Christophe Bailly

Christophe Bailly
Sorbonne Université | UPMC · Seed Biology Group - Laboratory of Developmental Biology

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About

115
Publications
45,477
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8,106
Citations
Citations since 2016
51 Research Items
4876 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Introduction
I am investigating the role of reactive oxygen species in seed biology, mainly as signaling compounds regulating dormancy and germination.
Additional affiliations
October 2007 - present
Université Paris 6 - UPMC - CNRS
January 2005 - present
January 2004 - present
University of Cape Town

Publications

Publications (115)
Preprint
High seed quality is key to agricultural production, which is increasingly affected by climate change. We studied the effects of drought and elevated temperature during seed production on key seed quality traits of two genotypes of malting barley ( Hordeum sativum L.). Plants of a “Hana-type” landrace (B1) were taller, flowered earlier and produced...
Article
Full-text available
In Arabidopsis thaliana, the breaking of seed dormancy in wild type (Col-0) by ethylene at 100 μL L−1 required at least 30 h application. A mutant of the proteolytic N-degron pathway, lacking the E3 ligase PROTEOLYSIS 6 (PRT6), was investigated for its role in ethylene-triggered changes in proteomes during seed germination. Label-free quantitative...
Article
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Climate change produces more frequent and intense drought events during seed development that can affect seed quality. Germination is critical to ensure plant growth and reproductive success but it can be impacted by various abiotic stresses. Here, we studied the effect of drought stress during sunflower seed development on the germination of the p...
Article
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A timely and efficient seed germination is critical for plantlets’ establishment and robustness as well as plant development and plant performance in both natural ecosystems and agrosystems [...]
Article
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In recent years, several reports pointed out the role of protein oxidation in seed longevity, notably regarding the oxidation of methionine (Met) residues to methionine sulfoxide (MetO) in proteins. To further consider this question, we present a handy proteomic method based on the use of two-dimensional diagonal electrophoresis (2Dd) and cyanogen...
Article
In barley, incubation of primary dormant (D1) grains on water under conditions that do not allow germination, i.e. 30°C in air and 15°C or 30°C in 5% O2, induces a secondary dormancy (D2) expressed as a loss of the ability to germinate at 15°C in air. The aim of this study was to compare the proteome of barley embryos isolated from D1 grains and D2...
Article
Reactive Oxygen Species (ROS) release seed dormancy through an unknown mechanism. We used different seed dormancy breaking treatments to decipher the dynamics and localization of ROS production during seed germination. We studied the involvement of ROS in the breaking of Arabidopsis seed dormancy by cold stratification, gibberellic acid (GA3) and l...
Article
Full-text available
In Arabidopsis seeds, ROS have been shown to be enabling actors of cellular signaling pathways promoting germination, but their accumulation under stress conditions or during aging leads to a decrease in the ability to germinate. Previous biochemical work revealed that a specific class of plastid thioredoxins (Trxs), the y-type Trxs, can fulfill an...
Article
Primary dormant seeds of Arabidopsis thaliana did not germinate in darkness at temperature higher than 10°C-15°C. Ethylene improved the germination of dormant wild type (Col-0) seeds at 25°C in darkness but seeds of the mutant affected in the proteolytic N-degron pathway, proteolysis6 (prt6), were insensitive to ethylene suggesting that PRT6 was in...
Article
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Underground early development of higher plants includes two distinct developmental processes, seed germination and then skotomorphogenesis, a mechanism which favours elongation of the hypocotyl and helps the seedling to find light. Interestingly, both processes, which are regulated by plant hormones, have been shown to depend on reactive oxygen spe...
Article
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Histone chaperones regulate the flow and dynamics of histone variants and ensure their assembly into nucleosomal structures, thereby contributing to the repertoire of histone variants in specialized cells or tissues. To date, not much is known on the distribution of histone variants and their modifications in the dry seed embryo. Here, we bring evi...
Article
At harvest sunflower seeds (Helianthus annuus L.) are dormant and poorly germinate at 10 and 25 °C. Seed sensitivity to low temperatures (10 °C) results from an embryo dormancy whereas the envelopes (pericarp and seed coats) inhibit the germination at 25 °C. Removal of pericarp greatly improved seed germination when the germination of whole achenes...
Article
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Ethylene and reactive oxygen species (ROS) regulate seed dormancy alleviation, but the molecular basis of their action and crosstalk remains largely unknown. Here we studied the mechanism of Arabidopsis seed dormancy release by ethylene using cell imaging, and genetic and transcriptomics approaches, in order to tackle its possible interaction with...
Article
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Background: Upon water uptake and release of seed dormancy, embryonic plant cells expand, while being mechanically constrained by the seed coat. Cortical microtubules (CMTs) are key players of cell elongation in plants: their anisotropic orientation channels the axis of cell elongation through the guidance of oriented deposition of load-bearing ce...
Article
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: Seed vigor is an important trait that determines seed performance in the field, which corresponds to seed germination rate and seedling establishment. Previous works brought helpful equations to calculate several parameters allowing vigor characterization. In this work we used base water potential (Ψb), base temperature (Tb) and seed lot (Ki) con...
Article
Seed germination is regulated by environmental factors, particularly water availability. Water deficits at the time of sowing impair the establishment of crop plants. Transcriptome and proteome profiling was used to document the responses of sunflower (Helianthus annuus) seeds to moderate water stress during germination in two hybrids that are nomi...
Article
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Reactive oxygen species (ROS) are versatile compounds which can have toxic or signalling effects in a wide range living organisms, including seeds. They have been reported to play a pivotal role in the regulation of seed germination and dormancy but their mechanisms of action are still far from being fully understood. In this review, we sum-up the...
Article
The accurate control of dormancy release and germination is critical for successful plantlet establishment. Investigations in cereals hypothesized a crucial role for specific MAP kinase (MPK) pathways in promoting dormancy release, although the identity of the MPK involved and the downstream events remain unclear. In this work, we characterized mut...
Article
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Background The functional determinants of H3K4me3, their potential dependency on histone H2B monoubiquitination, and their contribution to defining transcriptional regimes are poorly defined in plant systems. Unlike in Saccharomyces cerevisiae, where a single SET1 protein catalyzes H3K4me3 as part of COMPlex of proteins ASsociated with Set1 (COMPAS...
Preprint
Full-text available
The functional determinants of histone H3 Lys-4 trimethylation (H3K4me3), their potential dependency on histone H2B monoubiquitination (H2Bub) and their contribution to defining transcriptional regimes are poorly defined in plant systems. Unlike in S. cerevisiae, where a single SET1 protein catalyzes H3 Lys-4 trimethylation as part of COMPASS (COMP...
Article
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Temperature is the primary factor that affects seed dormancy and germination. However, the molecular mechanism that underlies its effect on dormancy alleviation remained largely unknown. In this study, we investigate hormone involvement in temperature induced germination as compared to that caused by after-ripening. Dormant (D) sunflower seeds cann...
Article
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Regulation of seed germination by dormancy relies on a complex network of transcriptional and post‐transcriptional modifications during seed imbibition that controls seed adaptative responses to environmental cues. High‐throughput technologies have brought significant progress in the understanding of this phenomena and have led to identify major re...
Article
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Dormant Arabidopsis (Arabidopsis thaliana) seeds do not germinate easily at temperatures higher than 10–15 °C. Using mutants affected in ethylene signaling (etr1, ein2 and ein4) and in the N-end-rule pathway of the proteolysis (prt6 and ate1-ate2) we have investigated the effects of cold and ethylene on dormancy alleviation. Ethylene (10–100 ppm) a...
Article
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10 °C, respe...
Article
Full-text available
Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate...
Article
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10 °C, respe...
Article
Full-text available
Global warming is a major agricultural issue in the Northern hemisphere where higher temperatures are expected to be associated with restricted water availability. In Europe, for maize, earlier and further northward sowings are forecasted in order to avoid water deficit periods in the crop life cycle. However these conditions may compromise seed ge...
Article
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The mother plant plays an important dynamic role in the control of dormancy of her progeny seed, in response to environmental signals. In order to further understand the mechanisms by which this dormancy control takes place we conducted a forward genetic screen to isolate mutants which fail to enter dormancy in response to variation in temperature...
Article
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The regulation of plant gene expression, necessary for development and adaptive responses, does not rely only on RNA transcription but also on mRNA fate. To understand whether seed germination relies on the degradation of specific subsets of mRNA we investigated whether the 5' to 3' RNA decay machinery participated in the regulation of this process...
Article
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Cuticular layers and seeds are prominent plant adaptations to terrestrial life that appeared early and late during plant evolution, respectively. The cuticle is a waterproof film covering plant aerial organs preventing excessive water loss and protecting against biotic and abiotic stresses. Cutin, consisting of crosslinked fatty acid monomers, is t...
Chapter
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Reactive oxygen species (ROS) have been shown to be toxic but also function as signaling molecules in a process called redox signaling. In seeds, ROS are produced at different developmental stages including dormancy release and germination. Main targets of oxidation events by ROS in cell are lipids, nucleic acids, and proteins. Protein oxidation ha...
Article
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Hydrogen sulfide (H2S) recently emerged as an important gaseous signaling molecule in plants. In this study, we investigated the possible functions of H2S in regulating Arabidopsis seed germination. NaSH treatments delayed seed germination in a dose-dependent manner and were ineffective in releasing seed dormancy. Interestingly, endogenous H2S cont...
Data
Effect of NaHS treatments on wild-type and des1 seed germination. WT and des1 seeds (50 per condition) were imbibed on paper filters soaked with distilled water containing 1 mM Na2SO4 (0) or increasing concentrations of NaHS. Germination was recorded after incubation at 15°C in the dark for 4 days. For all the conditions, 98–100% germination was ac...
Article
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The changes in germination potential of freshly harvested seeds of Arabidopsis thaliana stored in various combinations of temperature and relative humidity were investigated over 63 weeks of storage. Seeds of the wild type Col-0 and of two mutants displaying low and high levels of dormancy, cat2-1 and mtr4-1, respectively, were stored at harvest in...
Article
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Background and aims: Loss of seed viability has been associated with deteriorative processes that are partly caused by oxidative damage. The breaking of dormancy, a seed trait that prevents germination in unfavourable seasons, has also been associated with oxidative processes. It is neither clear how much overlap exists between these mechanisms no...
Article
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Dormancy is a complex evolutionary trait that temporally prevents seed germination thus allowing seedling growth at the favourable season. High throughput analyses of transcriptomes have led to significant progress in the understanding of the molecular regulation of this process but the role of post-transcriptional mechanisms has received little at...
Article
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The involvement of H2O2 generated by NADPH oxidase in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. In the wild-type, short-term salt exposure led to a transient and significant increase of H2O2 concentration, followed by a marked increase in catalase (CAT, EC 1.11.16), ascorbate peroxidase (APX, EC...
Article
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Ethylene is an important component of the gaseous environment, and regulates numerous plant developmental processes including seed germination and seedling establishment. Dormancy, the inability to germinate in apparently favorable conditions, has been demonstrated to be regulated by the hormonal balance between abscisic acid (ABA) and gibberellins...
Article
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Translatome profiling in dormant and nondormant sunflower (Helianthus annuus) seeds highlights post-transcriptional regulation of germination Summary Seed dormancy, which blocks germination in apparently favourable conditions, is a key reg-ulatory control point of plant population establishment. As germination requires de novo translation, its regu...
Article
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Sunflower (Helianthus annuus L.) seed dormancy is regulated by reactive oxygen species (ROS) and can be alleviated by incubating dormant embryos in the presence of methylviologen (MV), a ROS generating compound. Ethylene alleviates sunflower seed dormancy whereas abscisic acid (ABA) represses germination. The purposes of this study were to identify...
Article
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Orthodox seeds are living organisms that survive anhydrobiosis and may display dormancy, an inability to germinate at harvest. Seed germination potential can be acquired during a prolonged period of dry storage called after-ripening. The aim of this work was to determine if gene transcription is an underlying regulatory mechanism for dormancy allev...
Article
Germination of primary dormant barley grains is promoted by darkness and temperatures below 20°C, but is strongly inhibited by blue light. Exposure under blue light at 10°C for periods longer than 5 days, results in a progressive inability to germinate in the dark, considered as secondary dormancy. We demonstrate that the inhibitory effect of blue...
Article
Full-text available
Reactive oxygen species (ROS) are key players in the regulation of seed germination and dormancy. Although their regulated accumulation is a prerequisite for germination, the cellular basis of their action remains unknown, but very challenging to elucidate due to the lack of specificity of these compounds that can potentially react with all biomole...
Article
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In barley, primary dormant grains did not germinate at 30 °C in air and at 15 °C in an atmosphere containing less than 10% O2, while they germinated easily at 15 °C in air. O2 tension in embryos measured with microsensors was 15.8% at 15 °C but only 0.3% at 30 °C. Incubation of grains at 30 °C is known to induce secondary dormancy in barley, and it...
Data
Full-text available
Sunflower (Helianthus annuus L.) seeds progressively lost their ability to germinate at 25°C, the optimal temperature for germination, after accelerated aging was carried out at 45°C (a temperature too high to permit germination) in water or at 76 or 100% relative humidity (RH). The deleterious effects of the high‐temperature treatment increased wi...
Article
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Primary dormant barley (Hordeum vulgare) grains germinate at 10-15°C but not at 30°C, and there exist a positive correlation between embryo ABA content after 24 h on water and the depth of dormancy. Incubation at 30°C results in a progressive loss of the ability to germinate at 15°C. This induction of a secondary dormancy is optimal after 3 days an...
Article
Full-text available
Freshly harvested seeds of Arabidopsis thaliana, Columbia (Col) accession were dormant when imbibed at 25°C in the dark. Their dormancy was alleviated by continuous light during imbibition or by 5 weeks of storage at 20°C (after-ripening). We investigated the possible role of reactive oxygen species (ROS) in the regulation of Col seed dormancy. Aft...