Christina M. A. P. Schuh

Christina M. A. P. Schuh
University of Desarrollo | UDD · Clínica Alemana – UDD School of Medicine

PhD
Assistant Professor Centre for Regenerative Medicine / Vice Director PhD program "Science and Innovation in Medicine"

About

35
Publications
22,100
Reads
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364
Citations
Additional affiliations
January 2019 - present
University of Desarrollo
Position
  • Professor (Assistant)
August 2017 - December 2018
Universidad de Los Andes/ Cells for Cells
Position
  • PostDoc Position
April 2016 - October 2016
University College London
Position
  • PostDoc Position

Publications

Publications (35)
Article
Full-text available
Tissue engineering approaches in nerve regeneration often aim to improve results by bridging nerve defects with conduits that mimic key features of the nerve autograft. One such approach uses Schwann cell self-alignment and stabilisation within collagen gels to generate engineered neural tissue (EngNT). Here we investigated whether a novel blend of...
Article
Over the last decades exosomes have become increasingly popular in the field of medicine. While until recently they were believed to be involved in the removal of obsolete particles from the cell, it is now known that exosomes are key players in cellular communication, carrying source-specific molecules such as proteins, growth factors, miRNA/mRNA,...
Article
Full-text available
Microvesicles have become key players in cellular communication. Since glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative...
Article
Background and aim: The gold standard for peripheral nerve regeneration uses a sensory autograft to bridge a motor/sensory defect site. For motor nerves to regenerate, Schwann cells (SC) myelinate the newly grown axon. Sensory SCs have a reduced ability to produce myelin, partially explaining low success rates of autografts. This issue is masked i...
Article
Full-text available
Throughout the last decade, extracellular vesicles (EVs) have become increasingly popular in several areas of regenerative medicine. Recently, Apis mellifera royal jelly EVs (RJ EVs) were shown to display favorable wound healing properties such as stimulation of mesenchymal stem cell migration and inhibition of staphylococcal biofilms. However, the...
Preprint
Apis mellifera Royal Jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. Several active compounds have been identified, however, the mechanisms of action still remain widely unknown. As a glandular product, RJ h...
Article
Methylglyoxal (MGO) is an important molecule derived from glucose metabolism with the capacity of attaching to collagen and generating advanced glycation end products (AGEs), which accumulate in tissues over time and are associated with aging and diseases. However, the accumulation of MGO-derived AGEs in dentin and their effect on the nanomechanica...
Poster
Full-text available
A NOVEL HUMAN MATERIAL BASED PLATFORM TECHNOLOGY FOR TISSUE ENGINEERING This is the link to the supplemental video: https://youtu.be/cEArIBSnKsc
Article
Full-text available
Purpose: Recently, our group found exosome-like extracellular vesicles (EVs) in Apis mellifera honey displaying strong antibacterial effects; however, the underlying mechanism is still not understood. Thus, the aim of this investigation was to characterize the molecular and nanomechanical properties of A. mellifera honey-derived EVs in order to elu...
Article
Collagen is the most abundant structural protein in the body and the main component of the extracellular matrix of most tissues, including dentine and periodontal tissues. Despite the well-characterized role of collagen and specifically type-I collagen, as a ligand for host cells, its role as a substrate for bacterial adhesion and biofilm formation...
Article
Full-text available
Neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Creutzfeldt–Jakob disease (CJD) are brain conditions affecting millions of people worldwide. These diseases are associated with the presence of amyloid-β (Aβ), alpha synuclein (α-Syn) and prion protein (PrP) depositions in the brain, respectively, which lead t...
Article
Biofilm-mediated oral diseases such as dental caries and periodontal disease remain highly prevalent in populations worldwide. Biofilm formation initiates with the attachment of primary colonizers onto surfaces, and in the context of caries, the adhesion of oral streptococci to dentinal collagen is crucial for biofilm progression. It is known that...
Article
Full-text available
The diagnosis and management of pain is an everyday occurrence in dentistry, and its effective control is essential to ensure the wellbeing of patients. Most tooth-associated pain originates from the dental pulp, a highly vascularized and innervated tissue, which is encased within mineralized dentin. It plays a crucial role in the sensing of sti...
Article
Full-text available
To improve the outcome after autologous nerve grafting in the clinic, it is important to understand the limiting variables such as distinct phenotypes of motor and sensory Schwann cells. This study investigated the properties of phenotypically different autografts in a 6 mm femoral nerve defect model in the rat, where the respective femoral branche...
Article
Full-text available
Alzheimer’s disease (AD) is a neurodegenerative condition affecting millions of people worldwide. It is associated with cerebral amyloid-β (Aβ) plaque deposition in the brain, synaptic disconnection, and subsequent progressive neuronal death. Although considerable progress has been made to elucidate the pathogenesis of AD, the specific causes of th...
Article
Full-text available
Considerable advances have been made toward understanding the cellular and molecular mechanism of wound healing, however, treatments for chronic wounds remain elusive. Emerging concepts utilizing mesenchymal stem cells (MSCs) from umbilical cord, adipose tissue and bone marrow have shown therapeutical advantages for wound healing. Based on this pos...
Chapter
Laminins are major components of all basement membranes surrounding nerve or vascular tissues. In particular laminin-111, the prototype of the family, facilitates a large spectrum of fundamental cellular responses in all eukaryotic cells. Laminin-111 is a biomaterial frequently used in research, however it is primarily isolated from non-human origi...
Article
Full-text available
Background Cell-based therapies with autologous adipose tissue–derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progeni...
Conference Paper
Full-text available
Background: Recovery from peripheral nerve injuries is assessed with countless methods, but exact and reproducible assessment of functional regeneration is the most important criterion. To determine it may seem relatively straightforward in humans, but is rather difficult in animals, as those are unable to perform certain movements on command or to...
Article
Full-text available
Schwann cells play a key role in peripheral nerve regeneration. Failure in sufficient formation of Büngner bands due to impaired Schwann cell proliferation has significant effects on the functional outcome after regeneration. Therefore, the growth substrate for Schwann cells should be considered with highest priority in any peripheral nerve tissue...
Article
Full-text available
Damage to the peripheral nervous system (PNS) is a prevalent issue and represents a great burden to patients. Although the PNS has a good capacity for regeneration, regeneration over long distances poses several difficulties. Several recent studies have addressed Schwann cells' limited proliferative capacity; however, a solution has yet to be found...
Article
Background aims: As new approaches for peripheral nerve regeneration are sought, there is an increasing demand for native Schwann cells for in vitro testing and/or reimplantation. Extracorporeal shockwave treatment (ESWT) is an emergent technology in the field of regenerative medicine that has also recently been shown to improve peripheral nerve r...
Article
Full-text available
Therapeutic compensation of deficient bone regeneration is a challenging task and a topic of on-going search for novel treatment strategies. One promising approach for improvement involves non-viral gene delivery using the bone morphogenetic protein-2 (BMP-2) gene to provide transient, local and sustained expression of the growth factor. However, s...
Article
Tissue engineering approaches in nerve regeneration search for ways to support gold standard therapy (autologous nerve grafts) and to improve results by bridging nerve defects with different kinds of conduits. In this study, we describe electrospinning of aligned fibrin-poly(lactic-co-glycolic acid) (PLGA) fibers in an attempt to create a biomimick...
Article
Full-text available
Over the last decade, silk fibroin has been emergently used in peripheral nerve tissue engineering. Current approaches aiming to produce silk fibroin based nerve guidance conduits (SF-NGCs) used dissolved silk either based on aqueous solutions or organic solvents. In this study, we describe a novel procedure to produce SF-NGCs: a braided tubular st...
Article
Background aims Adipose-derived progenitor/stem cells (ASCs) are discussed as a promising candidate for various tissue engineering approaches. However, its applicability for the clinic is still difficult due to intra- and inter-donor heterogeneity and limited life span in vitro, influencing differentiation capacity as a consequence to decreased mul...
Article
Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell properties. In its decellularized form it has been successfully applied as nerve conduit biomaterial to improve peripheral nerve regeneration in injury models. We hypothesize that viable hAM without prior cell isolation can be differentiated towards the Schwann cell li...

Questions

Question (1)
Question
Hi everyone,
I have a question about an error message of our ultracentrifuge (Hanil 5.0):
We keep getting "error 50", when trying to start the centrifuge- but in the end it usually works after turning it off/on again.
However, we asume that error appears for a reason, and we´re worried about damaging the centrifuge. We tried manuals in all languages, but the last listed error is "error 45", nothing on error 50.
Does anybody have experience with Hanil ultracentrifuges and an idea what it could be?
Thanks in advance,
Christina

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Projects

Projects (3)
Project
We're interested in characterizing exosomes derived from apis mellifera honeybee regarding their antimicrobial and pro-regenerative effects.
Project
The aim of this project is to determine how approaches from regenerative medicine impact biofilm formation onto different substrates. Focus is placed on conditions such as wound healing, aging and oral diseases.
Project
Development and preclinical research focussed on effective delivery methods and expression systems for regenerative gene therapy in musculoskeletal defects