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Publications (15)
Amyloidosis is a disease group caused by pathological aggregation and deposition of peptides in diverse tissue sites. Apart from the fibril protein, amyloid deposits frequently enclose non-fibrillar constituents. In this study, carpal tunnel tissue sections with ATTR amyloid were analysed by quantitative mass spectrometry-based proteomics. Followin...
Top-down proteomics analyzes intact proteoforms with all of their post-translational modifications and genetic and RNA splice variants. In addition, modifications introduced either deliberately or inadvertently during sample preparation, that is, via oxidation, alkylation, or labeling reagents, or through the formation of noncovalent adducts (e.g.,...
Inflammasomes are cytosolic protein complexes, which orchestrate the maturation of active IL-1β by proteolytic cleavage via caspase-1. Although many principles of inflammasome activation have been described, mechanisms that limit inflammasome-dependent immune responses remain poorly defined. Here, we show that the thiol-specific peroxidase peroxire...
Spatial clustering of enzymes has proven an elegant approach to optimize metabolite transfer between enzymes
in synthetic metabolic pathways. Among the multiple methods used to promote colocalisation, enzyme fusion is
probably the simplest. Inspired by natural systems, we have explored the metabolic consequences of spatial
reorganizations of the ca...
The nematode Caenorhabditis elegans interacts with a variety of bacteria as it feeds on microbes, and a number of these both associate and persist within the worm's intestine. Host-microbe interactions in C. elegans have been analysed primarily at the transcriptome level with the host response often been monitored after challenge with pathogens. We...
Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a...
N-acyl homoserine lactones (AHL) are small signal molecules involved in the quorum sensing of many gram-negative bacteria, and play an important role in biofilm formation and pathogenesis. Present analytical methods for identification and quantification of AHL require time-consuming sample preparation steps and are hampered by the lack of appropria...
The invention relates to a compound having the chemical formula (I), wherein R1 represents the amino acids 1-120 of beta-lactoglobulin and R2 the amino acids 122-162 of beta-lactoglobulin from cow's milk. The invention further relates to a method for producing S-allylmercaptocysteine-modified beta-lactoglobulin, comprising the following steps: prov...
Relative protein abundances of Escherichia coli MG1655 growing exponentially on minimal medium with acetate or glucose as the sole carbon source were investigated in a quantitative shotgun proteome analysis with TMT6-plex isobaric tags. Peptides were separated by high resolution high/low pH 2D-LC, using an optimized fraction pooling scheme followed...
The whey protein β-lactoglobulin has been proposed as a transporter for covalent bound bioactive compounds in order to enhance their stability and reduce their sensory perception. The garlic derived compounds allicin and diallyl disulfide were bound covalently to the native and heat denatured protein. The binding site and the influence of the modif...
Die Erfindung betrifft Verbindung mit der chemischen Formel: wobei R1 die Aminosäuren 1–120 des β-Lactoglobulins und R2 die Aminosäuren 122–162 des β-Lactoglobulins aus Kuhmilch sind. Ferner betrifft die Erfindung ein S-Allylmercaptocystein modifiziertem β-Lactoglobulin-Herstellungsverfahren, aufweisend die Schritte:Bereitstellen einer wässrigen Lö...
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Caenorhabditis elegans can be infected by a plethora of pathogens, most of them are also pathogenic for humans. Consequently, the nematode has emerged as a powerful surrogate host to model microbial human infectious diseases in a non-vertebrate, for the study of innate immunity and host-pathogen interactions. Signaling cascades are wel...
Model organisms are an important tool for the development and validation of analytical approaches for proteomics and for the study of basic mechanisms of biological processes. The Initiative on Model Organism Proteomics (iMOP) organized a session during the 11th HUPO world congress in Boston in 2012, highlighting the potential of proteomics studies...
