Christian E. H. Schmelzer

Christian E. H. Schmelzer
Fraunhofer Institute for Microstructure of Materials and Systems IMWS | IWM · Business Unit Biological and Macromolecular Materials

PD Dr. rer. nat. habil.

About

89
Publications
33,292
Reads
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Introduction
Christian is head of a research department dedicated to future materials for the healthcare sector. He is an energetic, ambitious and creative person, driven by his passion for science. He considers himself as a 'forever student', eager to build on his strong academic foundations in engineering, physics, and life sciences. Christian is a purposeful leader and committed to the development of his employees and students. He has been teaching at a university for 10+ years.
Additional affiliations
July 2016 - present
Fraunhofer Institute for Microstructure of Materials and Systems IMWS
Position
  • Group Leader
January 2014 - June 2016
Martin Luther University Halle-Wittenberg
Position
  • Researcher
Description
  • Matrix Biology
February 2013 - December 2013
Technion - Israel Institute of Technology
Position
  • Researcher
Description
  • Proteomics, Peptidomics
Education
April 2003 - April 2007
Martin Luther University Halle-Wittenberg
Field of study
  • Ph.D. position
April 2002 - August 2002
Institute of Experimental Physics, University of Gdańsk
Field of study
  • Diploma
October 1998 - March 2003
University of Applied Sciences Merseburg
Field of study
  • Physical Engineering and Information Processing

Publications

Publications (89)
Article
Full-text available
MHC class I peptides are products of endogenous cellular protein degradation. Their prompt presentation, after rapid degradation of their newly synthesized source proteins, is needed to alert the immune system during pathogen infection. A possible source for such rapidly degrading proteins can be defective ribosome products (DRiPs), which include p...
Article
Background: This study aimed to investigate the prolyl and lysine hydroxylation in elastin from different species and tissues. Methods: Enzymatic digests of elastin samples from human, cattle, pig and chicken were analyzed using mass spectrometry and bioinformatics tools. Results: It was confirmed at the protein level that elastin does not con...
Article
Full-text available
Elastin is an essential vertebrate protein responsible for the elasticity of force-bearing tissues such as those of the lungs, blood vessels, and skin. One of the key features required for the exceptional properties of this durable biopolymer is the extensive covalent cross-linking between domains of its monomer molecule tropoelastin. To date, elas...
Article
Lysyl oxidases (LOXs) play a central role in extracellular matrix remodeling during development and tumor growth and fibrosis through cross-linking of collagens and elastin. We have limited knowledge of the structure and substrate specificity of these secreted enzymes. LOXs share a conserved C-terminal catalytic domain but differ in their N-termina...
Article
Elastin is an essential structural protein in the extracellular matrix of vertebrates. It is the core component of elastic fibers, which enable connective tissues such as those of the skin, lungs or blood vessels to stretch and recoil. This function is provided by elastin's exceptional properties, which mainly derive from a unique covalent cross‐li...
Article
In recent years, efforts have been made to develop special wound dressings to improve the healing of chronic or large area wounds. Elastin-gelatin-based nonwovens and sponges are candidates for these applications. Such materials are absorbable and possess a microstructure which is appropriate for the growth of biological cells. While sponges are su...
Article
Electrospun nonwovens have great potential for biomedical applications. They can be used, for example, to mimic the structure of the extracellular matrix of biological tissue. In this work, we demonstrate that the surface properties of nanofiber nonwovens made of biocompatible and very slowly biodegrading polyamide can be modified by UV picosecond...
Chapter
The vascular wall is a composite environment made of a myriad of different cell types and a complex extracellular matrix (ECM). The latter has a direct impact on vessel function and pathophysiological remodeling of ECM is associated with alterations of vessel structure and modifications of cell phenotype. In this context, elastin plays a fundamenta...
Article
Full-text available
The stratum corneum (SC) is the largest physical barrier of the human body. It protects against physical, chemical and biological damages, and avoids evaporation of water from the deepest skin layers. For its correct functioning, the homeostasis of the SC lipid matrix is fundamental. An alteration of the lipid matrix composition and in particular o...
Article
Polyelectrolyte multilayers (PEMs) consisting of the polysaccharides hyaluronic acid (HA) as the polyanion and chitosan (Chi) as the polycation were prepared with layer-by-layer technique (LbL). The [Chi/HA]5 multilayers were exposed to solutions of metal ions (Ca2+, Co2+, Cu2+ and Fe3+). Binding of metal ions to [Chi/HA]5 multilayers by the format...
Thesis
Die in dieser kumulativen Habilitationsschrift vorgestellten Arbeiten widmeten sich der Entstehung, den Eigenschaften und der Schädigung des extrazellulären Strukturproteins Elastin. Als Hauptkomponente der elastischen Fasern trägt es wesentlich zur strukturellen Integrität und Biomechanik dynamischer Gewebe von Vertebraten bei. Molekulare Untersuc...
Article
Full-text available
Elastic fibers are extracellular components of higher vertebrates and confer elasticity and resilience to numerous tissues and organs such as large blood vessels, lungs and skin. Their formation and maturation take place in a complex multistage process called elastogenesis. It requires interactions between very different proteins but also other mol...
Article
Full-text available
Extracellular matrix (ECM) is a dynamic 3-dimensional network of macromolecules that provides structural support for the cells and tissues. Accumulated knowledge clearly demonstrated over the last decade that ECM plays key regulatory roles since it orchestrates cell signaling, functions, properties and morphology. Extracellularly secreted as well a...
Article
Objective: TSP-1 (thrombospondin 1) is one of the most expressed proteins in platelet α-granules and plays an important role in the regulation of hemostasis and thrombosis. Interaction of released TSP-1 with CD47 membrane receptor has been shown to regulate major events leading to thrombus formation, for example, platelet adhesion to vascular endot...
Article
Full-text available
Biphasic calcium phosphate (BCP) materials are widely employed as bone substitute materials due to their resorption/degradation properties. Inflammation after implantation of such materials represents a prerequisite for bone tissue repair and regeneration but can be also problematic if it is not only transient and if it is followed by fibrosis and...
Article
Full-text available
Fibroblast to myofibroblast differentiation is a key feature of wound-healing in soft tissues, including the vagina. Vaginal fibroblasts maintain the integrity of the vaginal wall tissues, essential to keep pelvic organs in place and avoid pelvic organ prolapse (POP). The micro-environment of vaginal tissues in POP patients is stiffer and has diffe...
Article
Full-text available
Cationic liposomes composed of a novel lipid (N‐{6‐amino‐1‐[N‐(9Z) ‐octadec9‐enylamino] ‐1‐oxohexan‐(2S) ‐2‐yl} ‐N’‐ {2‐ [N, N‐bis(2‐aminoethyl) amino] ethyl} ‐2‐hexadecylpropandiamide) (OO4) and dioleoylphosphatidylethanolamine (DOPE) possess high amounts of amino groups and are promising systems for lipofection. Moreover, these cationic liposomes...
Article
Electrospinning is a well-established and widely used method for the production of protein-based fibrous biomaterials. The visualization of the morphology and the characterization of sample features related to the three-dimensional (3D) structure, like the porosity and fibers thickness, is crucial for the design and fabrication of tailor-made and a...
Article
Background Skin ageing is associated with structure-functional changes in the extracellular matrix, which is in part caused by proteolytic degradation. Since cysteine cathepsins are major matrix protein-degrading proteases, we investigated the age-dependent expression of elastolytic cathepsins K, S, and V in human skin, their in vitro impact on th...
Article
Full-text available
Elastic fibers are essential assemblies of vertebrates and confer elasticity and resilience to various organs including blood vessels, lungs, skin, and ligaments. Mature fibers, which comprise a dense and insoluble elastin core and a microfibrillar mantle, are extremely resistant toward intrinsic and extrinsic influences and maintain elastic functi...
Article
Full-text available
For parenteral controlled drug release, the desired zero order release profile with no lag time is often di�cult to achieve. To overcome the undesired lag time of the current commercial risperidone controlled release formulation, we developed PLGA–lipid microcapsules (MCs) and PLGA–lipid microgels (MGs). The lipid phase was composed of middle chain...
Article
Full-text available
Several spatial correlations of up to six fatty acid (FA) binding sites in albumins were found by double electron‐electron resonance (DEER). A strategy was used that combines spin‐labeling and spin‐probing techniques in electron paramagnetic resonance (EPR) spectroscopy. This is here achieved by introducing an additional covalent landmark spin (LS)...
Article
Full-text available
Freestanding multilayer films prepared by layer-by-layer technique have attracted interest as promising materials for wound dressings. The goal is to fabricate freestanding films using chitosan (CHI) and alginate (ALG) including subsequent crosslinking to improve the mechanical properties of films while maintaining their biocompatibility. Three cro...
Conference Paper
Full-text available
Electrospun nanofiber nonwovens have been studied for applications in regenerative medicine since several years. Their structure resembles the extracellular matrix protein fiber network in biological tissues. Approaches to use electrospun nonwovens as 3-dimensional cell carrier scaffolds are often hindered by the small pore size of the nonwovens. T...
Article
Full-text available
Matrix metalloproteinases are a class of enzymes, which degrade extracellular matrix components such as collagens, elastin, laminin or fibronectin. So far, four matrix metalloproteinases have been shown to degrade elastin and its precursor tropoelastin, namely matrix metalloproteinase-2, -7, -9 and -12. This study focuses on investigating the elast...
Article
Full-text available
Implants or cell carriers made of biopolymers or biodegradable polymers are well suited for the regeneration of defects in various tissues. They act as an adhesion surface for autologous cells and provide sufficient stability. Electrospun nonwovens have a favourable surface to volume ratio and mimic the structure of the fiber proteins of the extrac...
Conference Paper
Electrospun polylactide (PLA) nanofiber nonwovens are promising candidates for applications in tissue engineering. They may combine the advantageous chemical properties of PLA with a highly porous three-dimensional structure, which promotes the transportation of nutrients and the cell proliferation into the scaffolds. In this work, we tested differ...
Article
Laser interference lithography (LIL) and the layer-by-layer (LbL) technique are combined here for the first time to design a system with variable nanotopographies and surface viscoelasticity to regulate cell behavior. LIL is used to generate hexagonally arranged nanostructures of gold with different periodicity. In contrast, LBL is used to assemble...
Article
Full-text available
In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC–MS/MS of plasma m...
Article
We present an electron paramagnetic resonance (EPR) spectroscopic characterization of structural and dynamic effects that stem from posttranslational modifications of bovine serum albumin (BSA), an established model system for polymer-protein conjugation. Beyond the typical drug delivery and biocompatibility aspect of such systems, we illustrate th...
Article
Full-text available
A systematic study on the self-assembled solution system of human serum albumin (HSA) and paramagnetic doxyl stearic acid (5-DSA and 16-DSA) ligands is reported covering the broad pH range 0.7–12.9, mainly using electron paramagnetic resonance (EPR) methods. It is tested to which extent the pH-induced conformational isomers of HSA reveal themselves...
Article
Full-text available
Affecting more than 30% of the Western population, nonalcoholic fatty liver disease (NAFLD) is the most common liver disease and can lead to multiple complications, including nonalcoholic steatohepatitis (NASH), cancer, hypertension, and atherosclerosis. Insulin resistance and obesity are described as potential causes of NAFLD. However, we surmised...
Article
Rationale: α1-antitrypsin (AAT) deficiency-related emphysema is the fourth leading indication for lung transplantation. Chymotrypsin-like elastase 1 (Cela1) is a digestive protease that is expressed during lung development in association with regions of elastin remodeling, exhibits stretch-dependent expression during lung regeneration, and binds l...
Article
Neprilysin is also known as skin fibroblast-derived elastase, and its up-regulation during aging is associated with impairments of the elastic fiber network, loss of skin elasticity and wrinkle formation. However, information on its elastase activity is still limited. The aim of this study was to investigate the degradation of fibrillar skin elasti...
Preprint
α1-antitrypsin related lung disease (AAT-RLD) is the fourth leading indication for lung transplantation and is characterized by protease-mediated progressive emphysema that manifests in the 4 th or 5 th decade of life. Chymotrypsin-like elastase 1 ( Cela1 ) is a digestive enzyme that binds to elastin in a stretch-dependent manner and is covalently...
Article
Chronic skin conditions such as atopic dermatitis, psoriasis and aged skin are characterized by defective skin barrier and dryness which are associated with reduced levels of skin ceramides (CERs). The beneficial effects of plant-derived CERs for skin hydration and skin barrier recovery have been shown in several studies. Although plenty of glucosy...
Poster
Full-text available
Changes in tissue composition in premenopausal women have been linked to the pathophysiology of pelvic organ prolapse (POP). The aim of this study is to further our knowledge by performing extensive biochemical analyses of the main components of the extracellular matrix (ECM) in tissues from postmenopausal women with and without POP.
Conference Paper
Full-text available
Hypothesis / aims of study Changes in elastin content have been linked to the pathophysiology of pelvic organ prolapse (POP), however it is unclear whether the elastin content is increased [1] or decreased [2,3]. Such discrepancies could be attributed to differences in biopsy site, kind of analysis and/or patient population. Our study is the first...
Article
Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrinsic (chronological aging) and e...
Chapter
Soft connective tissues, such as skin, blood vessels, and lung and urogenital tissues, exhibit architecturally complex extracellular matrices (ECM) of which the elastic matrix forms a major structural component. This book is the first comprehensive resource on the subject, detailing state-of-the-art approaches to elastic matrix regeneration and rep...
Article
Full-text available
Williams–Beuren syndrome (WBS) is a congenital disorder, which involves the heterozygous deletion of the elastin gene and other genes on chromosome 7. Clinical symptoms that are associated with hemizygosity of the essential extracellular matrix protein elastin include premature aging of the skin and supravalvular aortic stenosis. However, only litt...
Article
Cadmium (Cd) is an important environmental pollutant and is poisonous to most organisms. We aimed to unravel the mechanisms of Cd toxicity in the model water plant Ceratophyllum demersum exposed to low (nM) concentrations of Cd as are present in nature. Experiments were conducted under environmentally relevant conditions, including nature-like ligh...
Article
Aging is a progressive process determined by genetic and acquired factors. Among the latter are the chemical reactions referred to as nonenzymatic posttranslational modifications (NEPTMs), such as glycoxidation, which are responsible for protein molecular aging. Carbamylation is a more recently described NEPTM that is caused by the nonenzymatic bin...
Article
Full-text available
Elastin is a vital protein of the extracellular matrix of jawed vertebrates and provides elasticity to numerous tissues. It is secreted in the form of its soluble precursor tropoelastin, which is subsequently cross-linked in the course of the elastic fiber assembly. The process involves the formation of the two tetrafunctional amino acids desmosine...
Article
Full-text available
This study aimed to characterize the structures of two elastin-like constructs, one composed of a cross-linked elastin-like polypeptide and the other one of cross-linked tropoelastin, and native aortic elastin. The structures of the insoluble materials and human aortic elastin were investigated using scanning electron microscopy. Additionally, all...
Article
The aim of this study was to explore the honeybee venom proteome applying a shotgun proteomics approach using different enrichment strategies (combinatorial peptide ligand libraries and solid phase extraction). The studies were conducted using nano-LC/MALDI-TOF/TOF-MS system. The MS analysis of peptide profiles (in the range of 900 - 4500 Da) and v...
Article
Post-translational modifications play a key role in defining the biological functions of proteins. Among them, the hydroxylation of proline producing the (2S,4R)-4-hydroxyproline (Hyp) is one of the most frequent modifications observed in vertebrates, being particularly abundant in the proteins of the extracellular matrix. In collagen, hydroxylatio...
Conference Paper
Full-text available
The velocity of ultrasound (c) and the density (ρ) have been measured in mixtures of water and polyethylene glycol (PEG) in the temperature range from 288.15 to 328.15 K. Based on the data obtained, the adiabatic compressibility coefficient (β_ad) has been calculated from Laplace's equation. The variations of c, ρ and β_ad with concentration (µ: mo...
Article
Full-text available
Although it has long been established that the extracellular matrix acts as a mechanical support, its degradation products, which mainly accumulate during ageing, have also been demonstrated to play an important role in cell physiology and the development of cardiovascular and metabolic diseases. In the present study, we showed that elastin-derived...
Article
Background: Elastin is a vital protein and the major component of elastic fibers which provides resilience to many vertebrate tissues. Elastin's structure and function are influenced by extensive cross-linking, however, the cross-linking pattern is still unknown. Methods: Small peptides containing reactive allysine residues based on sequences of...
Article
This study aimed to investigate the susceptibility of intact fibrillar human elastin to human leukocyte elastase (HLE) and cathepsin G (CG). Elastin is a vital protein of the extracellular matrix of vertebrates and provides exceptional properties including elasticity and tensile strength to many tissues and organs including aorta, lung, cartilage,...
Article
Aging and degeneration of human intervertebral disc (IVD) are associated with biochemical changes, including racemization and glycation. These changes can only be counteracted by protein turnover. Little is known about the longevity of IVD elastin in health or disease. Yet, such knowledge is important for a quantitative understanding of tissue synt...
Article
Full-text available
There is considerable evidence for specific pathology of lipid metabolism in schizophrenia, affecting polyunsaturated fatty acids and in particular sphingolipids. These deficits are assumed to interfere with neuronal membrane functioning and the development and maintenance of myelin sheaths. Recent studies suggest that some of these lipid pathologi...
Article
This study aimed to investigate the degradation of the natural substrates tropoelastin and elastin by the neutrophil-derived serine proteases human leukocyte elastase (HLE), proteinase 3 (PR3) and cathepsin G (CG). Focus was placed on determining their cleavage site specificities using mass spectrometric techniques. Moreover, the release of bioacti...
Article
In order to produce recombinant microbial transglutaminase (rMTG) which is free of the activating protease, dispase was used to activate the pro-rMTG followed by immobilized metal affinity chromatography (IMAC). As shown by MALDI-MS, the dispase does not only cleave the pro-sequence, but unfortunately also cleaves within the C-terminal histidine-ta...
Article
Aspergillus nidulans amine oxidase (ANAO) has the unusual ability among the family of copper and trihydroxyphenylalanine quinone-containing amine oxidases of being able to oxidize the amine side chains of lysine residues in large peptides and proteins. We show here that in common with the related enzyme from the yeast Pichia pastoris, ANAO can prom...
Article
The aim of this study was to characterize the two ceramide (CER) subclasses CER[NP] and CER[EOS] of human stratum corneum and to identify the chemical structures of their subspecies. High-performance thin-layer chromatography and normal-phase high-performance liquid chromatography were used for the separation of CER fractions, whereas nanoelectrosp...
Article
The aim of the study was to comprehensively characterize different honeybee venom samples applying two complementary mass spectrometry methods. 41 honeybee venom samples of different bee strains, country of origin (Poland, Georgia, and Estonia), year and season of the venom collection were analyzed using MALDI-TOF and nanoESI-QqTOF-MS. It was possi...