Christian Klein

Universitätsklinikum Tübingen · Poliklinik für Zahnerhaltung

Distinguishing composite remnants from tooth structure after trauma splint removal can be challenging. This study aimed to compare the Fluorescence-aided Identification Technique (FIT) with conventional light illumination (CONV) in terms of accuracy and time required for the detection of composite remnants after trauma splint removal. Ten bovine to...

Eine 67-jährige Brustkrebspatientin mit bekannten Metastasen in Sternum und Magen sowie dem Verdacht einer weiteren Metastasierung des Haupttumors in Richtung Leber und Pankreas stellte sich mit Beschwerden im rechten Unterkiefer vor. Die initiale Schmerztherapie basierte auf der Verdachtsdiagnose einer apikalen Parodontitis. Das im weiteren Behand...

Cell cultures can provide useful in vitro models. Since odontoblasts are postmitotic cells, they cannot be expanded in cell cultures. Due to their extension into the dentin, injuries are inevitable during isolation. Therefore, "odontoblast-like" cell culture models have been established. Nowadays, there is no accepted definition of odontoblast-like...

To reduce the risk of intra-operative haemorrhage during harvesting connective tissue from the palate and in order to assess the potential size of the soft tissue graft pre-operatively this study aims to evaluate the suitability of Doppler ultrasound scanning in detecting the greater palatine artery by non-invasive surface scanning and investigatin...

Objectives Publications on stamp techniques for placing resin-based composite (RBC) restorations consist mainly of case studies. Furthermore, comparative studies are rare and no longer relevant to the materials tested today. Thus, two general techniques were investigated in this study. Materials and methods Standardized occlusion class I cavities...

Objectives This study investigated the fluorescence properties of the most commonly used fluorescent CAD/CAM materials for monolithic dental restorations and their suitability to perform the fluorescence-aided identification technique (FIT). Materials and methods A total of 175 different color shades (n = 1) from 13 CAD/CAM material brands were an...

Purpose Molar-Incisor Hypomineralisation (MIH) remains a widespread developmental disorder of the teeth with a still largely unknown etiology. Perinatal events were blamed in previous studies for the development of MIH. The aim of the present study was to evaluate the influence of perinatal hypoxia—determined by the pH value of the umbilical cord b...

Objectives The autofluorescence of dental hard tissues has been known for over 100 years. Thus, manufacturers add fluorophores to dental restorative materials to improve the esthetic properties of these materials. So far, there has been no study evaluating the ability of these fluorophores to reproduce the autofluorescence of dental hard tissues....

The long-term preservation of fluorescence qualities of resin-based composite (RBC) restorations is an absolute condition for the implementation of the fluorescence-aided identification technique (FIT) in dentistry and forensic medicine. Therefore, this study assessed the fluorescence of 244 color shades of 16 commercially available RBC brands with...

Objectives This study investigated the ability of the fluorescence-aided identification technique (FIT) facilitated by a novel handpiece to simplify the removal of tooth-colored composite restorations with water-cooled rotating instruments. Materials and methods Five undergraduate students and five dentists (6–14 years of professional experience)...

Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of col...

The aim of this study was to determine singular fluorescence qualities of different, commercially available resin composites shades. A total of 234 brand name colors including enamel, dentin and special shades were examined using a monochromator-based spectrophotometer. From the examined composites, FiltekTM Z250 (867±279) RFU and Supreme XT (denti...

Bone regeneration in critical size defects is a major challenge in oral and maxillofacial surgery, and the gold standard for bone reconstruction still requires the use of autologous tissue. To overcome the need for a second intervention and to minimize morbidity, the development of new biomaterials with osteoinductive features is the focus of curre...

The objective of the study was to analyse Streptococcus mutans biofilms grown under different dietary conditions by using multifaceted methodological approaches to gain deeper insight into the cariogenic impact of carbohydrates. S. mutans biofilms were generated during a period of 24 h in the following media: Schaedler broth as a control medium con...

The goal of our research work is to establish mesenchymal osteoprogenitors derived from human jaw periosteum for tissue engineering applications in oral and maxillofacial surgery. For future autologous and/or allogeneic transplantations, some issues must be addressed. On the one hand, animal-free culture conditions have yet to be established. On th...

The objective was to assess the fluorescence properties of current commercial resin composites. Sixteen light-curing resin composites, representing a total number of 241 shades, were analyzed. Fluorescence measurements of all samples were taken using the monochromator-based fluorescence reader Synergy(TM )Mx (BioTek Instruments Inc. ). Additionally...

The aim of our study was to generate a biofunctionalized, three-dimensional (3D) biomaterial to enhance jaw periosteal cell (JPC) adhesion and differentiation into osteogenic tissue. Therefore, open-cell polylactic acid (OPLA) scaffolds were coated covalently with different RGD peptides (a conserved recognition sequence of the most ECM proteins--ar...

Aim To assess the accuracy of Cone Beam Computed Tomography (CBCT) in determining root canal length ex vivo. Methodology The real lengths of 25 root canals were determined under a stereomicroscope, Stemi 2000 c (Carl Zeiss GmbH, Germany) with 4· magnification. A file was introduced into the root canal until the tip was flush to the apical foramen....

Caries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-soluble EPS produced by S. mutans during several gro...

The purpose of the present study was to evaluate the Reamer/Irrigator/Aspirator (RIA), a reaming system designed to incorporate the advantages of irrigation and suction for every day clinical use. The evaluation process was focused on the displacement of the medullary content and its impact on cortical perfusion. The results of the RIA reamed naili...

OBJECTIVES: The aim of this study was to assess the fluorescence properties of current commercial composites in comparison to compomers, glass ionomer cements, dentin and enamel. METHODS: Sixteen light-curing resin composites (i.e. Tetric EvoCeram, Empress Direct (Ivoclar-Vivadent); Esthet-X HD, Ceram-X Duo, Spectrum (Dentsply); EcuSphere (DMG); F...

LEHRFELD: Die Parodontitis ist eine multifaktorielle Erkrankung des Zahnhalteapparates, verläuft schleichend und schmerzfrei, und stellt in der zweiten Lebenshälfte die häufigste Ursache für Zahnverlust dar (Glockmann 1998, Splieth 2002). Die Planung und Therapie erfordert übergreifende Kenntnisse in konservierender, chirurgischer und prothetischer...

Isolated jaw periosteum-derived cells (JPCs) comprise a morphologically heterogeneous population. There are no known specific surface markers that are able to distinguish between progenitors and cells of other tissue types. The aim of our study was to identify differentiation markers as predictors of JPC mineralization capacity. JPCs underwent oste...

To compare the preparation quality of two rotary systems and NiTi-hand files in oval root canals, and to evaluate the effect of canal dimensions on the preparation. Ninety roots with oval root canals were selected. The middle third was cross sectioned at two levels and photographed. The maximum and minimum diameters of the root and canal were recor...

During tooth development, several different mineralization processes take place. The tooth is a functional system of dental hard and soft tissues, and the formation of teeth underlies genetic and subsequent cellular control. Mineralization arises by the occurrence of matrix vesicles, followed subsequently by extracellular matrix mineralization proc...

IntroductionBasic OdontogenesisDentinogenesisPre-conditions for Dentinogenesis as Model for Biomineralization ProcessesMethods and ResultsAcknowledgmentsReferences

Ziel der Arbeit war die Bewertung des „Reamer Irrigator Aspirator“-Prototyps (RIA), eines mit Spülen und Absaugen arbeitenden Markraumbohrers, in Bezug auf seine Effektivität betreffend der Reduktion der Fettverpressung und seine Auswirkungen auf die Durchblu-tung und Frakturheilung.Als Vergleich diente der ungebohrte, verriegelte AO-Nagel (Ø 7,5 m...