Christian Chervin

Christian Chervin
Institut National Polytechnique de Toulouse | INPT · ENSAT

PhD + Dipl. Oenol.

About

101
Publications
21,185
Reads
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3,553
Citations
Citations since 2017
25 Research Items
2020 Citations
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20172018201920202021202220230100200300400
20172018201920202021202220230100200300400
Additional affiliations
September 1998 - December 2018
École Nationale Supérieure Agronomique de Toulouse
Position
  • Professor (Full)

Publications

Publications (101)
Article
Full-text available
Auxin is known to regulate cell division and cell elongation, thus controlling plant growth and development. Part of the auxin signaling pathway depends on the fine-tuned degradation of the auxin/indole acetic acid (Aux/IAA) transcriptional repressors. Recent evidence indicates that Aux/IAA proteins play a role in fruit development in tomato (Solan...
Article
Glycosides are an important potential source of aroma and flavour compounds for release as volatiles in flowers and fruit. The production of glycosides is catalysed by UDP-glycosyltransferases (UGTs) that mediate the transfer of an activated nucleotide sugar to acceptor aglycones. A screen of UGTs expressed in kiwifruit (Actinidia deliciosa) identi...
Article
Full-text available
Auxin is one of the most prominent phytohormones regulating many aspects of fleshy fruit development including fruit set, fruit size through the control of cell division and cell expansion, and fruit ripening. To shed light on the role of auxin fruit ripening, we have previously shown that Sl-ARF4 is a major player in mediating the auxin control of...
Article
Tomato (Solanum lycopersicum) quality traits such as juice soluble solid content (Brix), juice pH, color parameters (Hue and Chroma), firmness and water content, are critical factors for fruit quality assessment. The need for screening very large numbers of fruit has led to the development of a high-throughput method using visible-near infrared (VI...
Article
Full-text available
Successful completion of fruit developmental programs depends on the interplay between multiple phytohormones, yet, beside ethylene the impact of other hormones on fruit quality traits remains elusive. A previous study has shown that down-regulation of Sl-ARF4, a member of the tomato (Solanum lycopersicum). Auxin Response Factor (ARF) gene family,...
Preprint
Full-text available
Ethylene is known to stimulate plant respiration, and this later is always associated with heat generation. The grapevine fruit set is dependent upon pistil temperature, controlling the pollen germination and ovule fertilization. This led us to test whether ethylene would be able to impact fruit set often limited in cool climate conditions, particu...
Article
A recent method using paired comparison between coded wines and a pivot wine, first developed with wine experts, was adapted to be performed by students, as potential young consumers. The untrained students generated the list of words in a preliminary session, and then a paired comparison with a pivot wine was performed, using the words representin...
Conference Paper
Full-text available
Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing su...
Article
The plant hormone ethylene plays vital roles in plant development, including pollen tube (PT) growth. Many studies have used the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), as a tool to trigger ethylene signaling. Several studies have suggested that ACC can act as a signal molecule independently of ethylene, inducing responses...
Article
Ethylene modulates plant developmental processes including flower development. Previous studies have suggested ethylene participation in pollen tube (PT) elongation, and both ethylene production and perception seem critical at fertilization time. The full gene set regulated by ethylene during PT growth is unknown. To study this, we used various ETh...
Article
Full-text available
One irreversible consequence of acidic pH for roots is cell death. Growing evidence suggests the role of hormones and cell wall-related enzymes in response to acidic pH that could possibly avoid cell mortality. Here, we have investigated the role of ethylene and class III peroxidases (CIII Prxs) activity on sensitivity to further low pH treatment....
Article
Full-text available
3-isobutyl-2-methoxypyrazine (IBMP) is a grape-derived aroma compound responsible for the bell pepper character of wine. It is still unclear whether this molecule is always negatively perceived by consumers. The objective of this study was to establish a consumer rejection threshold (CRT) for IBMP in French white and red wines from the Gaillac area...
Article
Full-text available
Arbuscular mycorrhizal symbiosis is a mutualistic interaction between most land plants and fungi of the glomeromycotina subphylum. The initiation, development and regulation of this symbiosis involve numerous signalling events between and within the symbiotic partners. Among other signals, phytohormones are known to play important roles at various...
Chapter
Small molecules of natural origin have been reported to act as alternatives to synthetic fungicides. These are reviewed in this chapter, and some ideas of new development are given. The list, which is not exhaustive, is the following: acetaldehyde, acetic acid, aldehydes (other than acetaldehyde), aminobutyric acids, ascorbic acid, ethanol, ethylen...
Preprint
Arbuscular Mycorrhizal (AM) symbiosis is a mutualistic interaction between most land plants and fungi of the glomeromycotina subphylum. The initiation, development and regulation of this symbiosis involve numerous signalling events between and within the symbiotic partners. Among other signals, phytohormones are known to play important roles at var...
Article
With the forecasted fast increase in world population and global climate change, providing sufficient amounts of quality food becomes a major challenge for human society. Seed and fruit crop yield is determined by developmental processes including flower initiation, pollen fertility and fruit set. Fruit set is defined as the transition from flower...
Article
Full-text available
Ethylene regulates many aspects of plant growth and development. It is perceived by a family of ethylene receptors (ETRs) that have been well described. However, a full understanding of ETR function is complicated by functional redundancy between the receptor isoforms. Here, we characterize a new ETR, SlETR7, that was revealed by tomato genome sequ...
Article
Ethanol is known to accumulate in various plant organs under various environmental conditions. However, there are very scarce data about ethanol sensing by plants. We observed that ethanol accumulates up to 3.5 mM during tomato seed imbibition, particularly when seeds were stacked. Stacked seeds germinated less than spread out seeds suggesting etha...
Article
With the forecasted fast increase in world population and global climate change, providing sufficient amounts of quality food becomes a major challenge for human society. Seed and fruit crop yield is determined by developmental processes including flower initiation, pollen fertility and fruit set. Fruit set is defined as the transition from flower...
Article
Full-text available
Aim: Grape growers sometimes use cyanamides (calcium or hydrogen) to release bud dormancy in warm climate regions, where the chilling requirement has not been met during winter. However, these products can cause damage to plants and are dangerous to handle, so alternatives would be welcomed by growers. Connections between metabolisms of ethanol, et...
Article
Full-text available
Ethylene regulates fruit ripening and several plant functions (germination, plant growth, plant-microbe interactions). Protein quantification of ethylene receptors (ETRs) is essential to study their functions, but is impaired by low resolution tools such as antibodies that are mostly nonspecific, or the lack of sensitivity of shotgun proteomic appr...
Article
A complex network of pathways coordinates nodulation and epidermal root hair infection in the symbiotic interaction between rhizobia and legume plants. Whereas nodule formation was known to be autoregulated, it was so far unclear whether a similar control is exerted on the infection process. We assessed the capacity of Medicago plants nodulated by...
Conference Paper
Full-text available
Digital technology has revolutionized the way we communicate. It should fundamentally transform the way we teach. Since late 2011, the rise of MOOCs (massive open online courses) has gradually redefined both access to and the design of higher education worldwide. In this context, the French government adopted an ambitious roadmap for digital teachi...
Chapter
The fruit ripening is the latest part of fruit development, leading to fruit that attracts seed dispersers (humans and other animals), because of color change, sweet taste, nice odors, among other traits. The ethylene is a simple two-carbon gas, that is produced by plants, among various organisms, and enhances fruit ripening. The ethylene biosynthe...
Article
Full-text available
Ethylene is a gaseous plant hormone perceived by a family of receptors in Arabidopsis thaliana including ETHYLENE RESPONSE1 (ETR1) and ETR2. Previously we showed that etr1-6 loss-of-function plants germinate better and etr2-3 loss-of-function plants germinate worse than wild-type under NaCl stress and in response to abscisic acid (ABA). In this stu...
Book
The fruit ripening is the latest part of fruit development, leading to fruit that attracts seed dispersers (humans and other animals), because of color change, sweet taste, nice odors, among other traits. The ethylene is a simple two-carbon gas, that is produced by plants, among various organisms, and enhances fruit ripening. The ethylene biosynthe...
Article
Pyoverdines are siderophores synthesized by fluorescent pseudomonas spp. Under iron-limiting conditions, these high-affinity ferric iron chelators are excreted by bacteria in the soil to acquire iron. Pyoverdines produced by beneficial Pseudomonas ameliorate plant growth. Here, we investigate the physiological incidence and mode of action of pyover...
Article
Full-text available
The plant hormone ethylene plays a key role in climacteric fruit ripening. Studies on components of ethylene signaling have revealed a linear transduction pathway leading to the activation of ethylene response factors. However, the means by which ethylene selects the ripening-related genes and interacts with other signaling pathways to regulate the...
Article
Auxin is known to be involved in all the stages of fruit development. Aux/IAAs are regulators of the auxin signaling at the transcription level. In a recent study, using RNAi strategy to limit the expression Sl-IAA17, it was shown that this tomato AuxIAA regulates fruit size mainly through altering the ploidy level of pericarp cells. Indeed, Sl-IAA...
Article
Full-text available
Background Tomato fruit ripening is controlled by ethylene and is characterized by a shift in color from green to red, a strong accumulation of lycopene, and a decrease in β-xanthophylls and chlorophylls. The role of other hormones, such as auxin, has been less studied. Auxin is retarding the fruit ripening. In tomato, there is no study of the caro...
Conference Paper
Full-text available
At present, spectrum analysis is predominantly employed to characterize the mature stage of fruits and vegetables in laboratory condition. It is well known that microwave permittivity is an important characteristic of materials. Measuring the permittivity of vegetables and fruits will be a potential supplementary method. In this paper, two sensors,...
Article
As a consequence of the non-climacteric status of grapes (Vitis vinifera), ethylene biosynthesis and signal transduction have scarcely been studied in this fruit. In spite this drawback, the available information suggests a role for ethylene in ripening grape berries. In this work, we report the identification of three homologous genes that encode...
Chapter
Full-text available
Several small molecules of natural origin have been shown to act as alternatives to synthetic fungicides for biological control of fungal diseases of grapes and other crops. The small molecule chemicals discussed here include: acetaldehyde, acetic acid, aldehydes (other than acetaldehyde), ascorbic acid, ethanol, ethylene, jasmonic acid and methyl...
Chapter
Introduction, botany, cultivation and product statisticsMorphology and physiologyPost-harvest technology for wine and juice grapesPost-harvest technology for dried grapesPost-harvest technology for table grapes
Article
Full-text available
‘Fallglo’ is a popular tangerine (Citrus reticulata Blanco) cultivar with high eating quality. However, ‘Fallglo’ may contain as many as 30–40 seeds per fruit. ‘US Early Pride’ is a seedless mutation of ‘Fallglo’ with similar quality attributes. The objective of the current study was to determine if ‘Fallglo’ and ‘US Early Pride’ fruit differed in...
Article
This book has 19 chapters focusing on the beneficial effects of the consumption of fruits and vegetables on human health. Some of the most common fruits and vegetables, their biologically active constituents and their medicinal properties are discussed. Some methodologies used for the extraction, isolation, characterization and quantification of th...
Article
Full-text available
There are several studies suggesting that tomato (Solanum lycopersicum) chromoplasts arise from chloroplasts, but there is still no report showing the fluorescence of both chlorophylls and carotenoids in an intermediate plastid, and no video showing this transition phase. Pigment fluorescence within individual plastids, isolated from tomato fruit u...
Article
Volatile esters are key compounds of kiwifruit flavour and are formed by alcohol acyltransferases that belong to the BAHD acyltransferase superfamily. Quantitative RT-PCR was used to screen kiwifruit-derived expressed sequence tags with proposed acyltransferase function in order to select ripening-specific sequences and test their involvement in al...
Article
Full-text available
Chromoplasts are nonphotosynthetic plastids that accumulate carotenoids. They derive from other plastid forms, mostly chloroplasts. The biochemical events responsible for the interconversion of one plastid form into another are poorly documented. However, thanks to transcriptomics and proteomics approaches, novel information is now available. Data...
Article
The volatile compounds that constitute the fruit aroma of ripe tomato (Solanum lycopersicum) are often sequestered in glycosylated form. A homology-based screen was used to identify the gene SlUGT5, which is a member of UDP-glycosyltransferase 72 family and shows specificity towards a range of substrates, including flavonoid, flavanols, hydroquinon...
Article
Full-text available
Chromoplasts are carotenoid-accumulating plastids conferring color to many flowers and fruits as well as to some tubers and roots. Chromoplast differentiation proceeds from preexisting plastids, most often chloroplasts. One of the most prominent changes is remodeling of the internal membrane system associated with the formation of carotenoid-accumu...
Article
Full-text available
The ethylene signalling pathway has never been fully described in grapes. Regarded as a non-climacteric fruit, grape berry seems to ripen independently to ethylene, however 1-methylcyclopropene (1-MCP), a specific inhibitor of ethylene receptors has been shown to alter berry ripening processes. Here, we report profiles of transcript abundance of va...
Article
Preharvest applications of a 16% ethanol (EtOH) solution, containing 1 % of calcium chloride (CaCl2), reduced gray mold development in ‘Chasselas’ table grapes picked at a late harvest date, the losses due to rotten clusters dropped from 15% in controls to 5% in grapes treated with EtOH+CaCl2. Then over a 6-week cold storage, the losses due to gray...
Article
Full-text available
The UDP glucose-flavonoid 3-O-glucoslyltrans-ferase (UFGT) is a key enzyme for biosynthesis and stability of anthocyanin pigments of red grapes. Un-derstanding factors affecting expression of this en-zyme is thus important for the control of grape colour. A 1640 bp promoter region of the grapevine ufgt gene was cloned and sequenced. Sequence analys...
Article
Grape is considered as a non-climacteric fruit, the maturation of which is independent of ethylene. However, previous work had shown that ethylene is capable of affecting the physiological processes during maturation of grape berries. Experiments were designed to screen the gene pool affected by ethylene at the ripening inception in Cabernet Sauvig...
Book
Le rapport de l'expertise scientifique collective Inra (novembre 2007) disponible sur le site de l'Institut, comprend les 1 330 références bibliographiques des publications scientifiques citées dans le rapport.
Article
Full-text available
Stimulation by ethylene of the UDP glucose-flavonoid 3-O-glucosyltransferase (UFGT) in grape tissues is independent from the MybA transcription factors
Article
Shredded carrots were either chlorinated, rinsed, and spin-dried as in industrial processes, or irradiated (2 kGy), replacing the three steps. Several factors defining the quality of minimally processed vegetables were monitored during storage at 10°. Atmospheres inside micro-porous plastic bags stabilized at 7–15% O2 and 10–15% CO2. Sugar levels i...
Article
The effects of radiation-mediated free radical production on polyamine metabolism were investigated in grape cells (Vitis vinifera L. cv. Gamay) using a cell suspension culture. Putrescine (Put) synthesis was triggered in irradiated cells (0. 5 kGy) only when ammonium was present in the culture medium. Under these conditions. Put accumulated to a c...
Article
Full-text available
*Purpose of the review: The involvement of ethylene in fruit ripening has been the subject of intensive molecular and biochemical studies over the last twenty years. Whereas new methods and new genes are being discovered, the differences between climacteric and non climacteric fruit seem to decrease. Recent studies are showing potential roles for e...
Article
Grape ripening is thought to be ethylene independent. However, Cabernet Sauvignon berries that were treated with 1-methylcyclopropene (1-MCP), a specific inhibitor of ethylene receptors, accumulated less sucrose over the following three weeks than did controls. This was associated with a decreased RNA accumulation of two sucrose transporters (SUC11...
Conference Paper
Full-text available
Alcohol dehydrogenase (ADH) has been considered for a long time a stress-responsive gene, but renewed interest in this marker arose when it was found to be involved in organ development. The VvADH2 gene is one of the ripeningrelated genes and its expression increases from the onset of ripening (véraison) until fruit maturity. Little information is...
Article
Full-text available
While grapes have been classified as a non-climacteric fruit, we show here that endogenous ethylene production just before veraison is required for an increase in berry size and possibly for anthocyanin accumulation in the ripening berry. Our data also show that the peak of ethylene production just prior to veraison is associated with increased acc...
Article
The application of ethanol vapours has been optimised over two seasons in order to prevent rot development, caused by Botrytis cinerea, and stem browning in ‘Chasselas’ table grapes. At a dose rate of 2 ml kg−1 of grapes, ethanol vapour was as effective as sulphur dioxide pads. Consumer panels detected no significant difference in sensory perceptio...
Article
Full-text available
Spraying aqueous solutions of ethanol (between 2.5 and 10%, v/v) 1 month before harvest has been shown to increase berry weight of Cabernet Sauvignon by about 10% at harvest compared with controls sprayed with water. These observations were made in France and in Australia. The increase in berry weight was not associated with significant changes in...
Article
While the grape has been classified as a non-climacteric fruit whose ripening is thought to be ethylene independent, we show here that a transient increase of endogenous ethylene production occurs just before veraison (i.e. inception of ripening). We observed that ethylene perception, at this time, is required for at least the increase of berry dia...
Article
Full-text available
Although grape berries have been classified as non-climacteric fruits, ongoing studies on grape ethylene signalling challenge the role of ethylene in their ripening. One of the significant molecular changes in berries is the up-regulation of ADH (alcohol dehydrogenase, EC 1.1.1.1) enzyme activity at the inception of fruit ripening and of VvADH2 tra...
Article
Full-text available
Three different ethanol solutions were sprayed onto Cabernet Sauvignon (Vitis vinifera L.) clusters during the ripening period: 2.5, 5 and 10% by volume in water. Controls were sprayed with water alone. Three different times of spraying were also tested: 8, 10 and 13 weeks post-flowering. One of the observed changes was a lower titratable acidity i...
Article
Full-text available
We have shown previously that ethanol vapours (given by 2 ml per kg of grapes) can prevent Botrytis development and stem browning, two of the major problems in postharvest quality of table grapes. In the present paper, we will give emphasis to preliminary results about (i) the role of ethanol vapours in the inhibition of berry shatter and (ii) the...
Article
Full-text available
Recent studies have shown that dipping table grapes in ethanol solutions at harvest improved storage of the fruit. We report here the first results obtained by treating "Chasselas" table grapes (Vitis vinifera) with ethanol vapours over the storage period. We tested the effect of ethanol at 0, 4 and 8 g/kg fruit during cold storage for 2, 4 and 6 w...
Article
The treatment of grape berries (Vitis vinifera L. cv. Cabernet Sauvignon) with the ethylene-releasing compound, 2-chloroethylphosphonic acid (2-CEPA), at veraison is a method known to enhance grape skin colour. We observed that it produced a 6-fold increase, up to 30 pmol g1 FW, of the cluster internal ethylene compared to untreated controls within...
Article
Eutypine, 4-hydroxy-3-(3-methyl-3-butene-1-ynyl) benzaldehyde, is a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in grapevine. The effect of the toxin on anthocyanin synthesis has been investigated in Vitis vinifera cv. Gamay cell cultures. At concentrations higher than 200 micromol/L, eutypine reduced anthocyanin accumulation...
Article
Full-text available
Early picked "Granny Smith" apples (Malus domestica Borkh.) were stored under air or CA (controlled atmosphere at 2 kPa O2 and <1 kPa CO2) at 1°C. During the first week of storage, fruit was subjected to ethanol vapours in doses from 0 to 8 g/kg of fruit. Ethanol at 4 g/kg protected fruit against superficial scald in CA storage for at least 5.5 mon...
Article
Recent studies have shown that low doses of ethanol stimulate the maturation of some fruits. The present work showed that spraying Cabernet Sauvignon grapes, with 5% ethanol at veraison enhances the anthocyanin accumulation. Veraison is the time when the berries turn from green to purple. HPLC analysis showed a marked increase in the total concentr...
Article
Full-text available
Spraying ethanol (5% v/v in water)onto grape clusters at mid-veraison led to a 30 % drop in the malic acid concentration at harvest. As a consequence, tritable acidity also dropped by 10 %. The concentration of tartaric acide did not change significantly. The mode of action of ethanol on malic acid metabolism is discussed.
Article
Aqueous ethanol (5% v/v) was sprayed onto bunches of Vitis vinifera L. cv. Cabernet Sauvignon 8–9 weeks following full flowering (controls were sprayed with water). Internal ethylene concentration in treated berries increased to a maximum within 6 hours, and berry colour subsequently increased substantially (maximum attained 2–3 weeks after sprayin...
Article
Full-text available
The effects of ethanol vapors, controlled atmosphere (CA) storage, and a combination of both on superficial scald development on "Granny Smith" apples (Malus ×domestica Borkh.) are reported. The major result was that ethanol vapors, applied in cold storage, prevented scald development over a week at 20 °C in apples that had been CAstored for 4 mont...
Article
We evaluated the efficacy of ethanol vapour treatment to control scald of Granny Smith apples, because diphenylamine treatment is not permitted by certain importing countries. In the first year, in a simple preliminary experiment on bagged fruits, ethanol at 3 g/kg, reduced scald compared with controls. In the second year, we observed that ethanol...
Article
Controlled atmosphere storage is known to decrease pome fruit aroma. Here results are presented showing that low O2 storage (3 kPa) for 2 months reduced character impact compounds of ‘Packham’s Triumph’ pears, namely methyl and ethyl decadienoates, during subsequent ripening. This reduction was detected in both whole pears and crushed pear flesh, u...
Article
Full-text available
Insect feeding traces on fruit are a major concern to orchardists. Breeding fruit for insect resistance is becoming more important as available pesticides are limited by more stringent regulations, problems of insect resistance, and residue limits. We present a method to analyze fruit skin damage via treatment of video images. This aspect has not b...