Christian Brede

Christian Brede
University of Wuerzburg | JMU · Department of Internal Medicine II

PhD

About

66
Publications
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723
Citations

Publications

Publications (66)
Article
We describe a new species of the genus Conraua from the Fouta Djallon Highlands in Guinea. The species is recognised as distinct from nominotypical C. alleni, based on morphological evidence and is supported by a recent species delimitation analysis, based on DNA sequence data. The new species is distinguished from its congeners by the unique combi...
Article
Full-text available
We describe a new species of the genus Conraua from the Fouta Djallon Highlands in Guinea. The species is recognised as distinct from nominotypical C. alleni , based on morphological evidence and is supported by a recent species delimitation analysis, based on DNA sequence data. The new species is distinguished from its congeners by the unique comb...
Article
Full-text available
Acute graft-versus-host disease (aGvHD) is a severe and often life-threatening complication of allogeneic hematopoietic cell transplantation (allo-HCT). AGvHD is mediated by alloreactive donor T-cells targeting predominantly the gastrointestinal tract, liver, and skin. Recent work in mice and patients undergoing allo-HCT showed that alloreactive T-...
Article
Full-text available
The use of animal models of infection is essential to advance our understanding of the complex host-pathogen interactions that take place during Aspergillus fumigatus lung infections. As in the case of humans, mice need to suffer an immune imbalance in order to become susceptible to invasive pulmonary aspergillosis (IPA), the most serious infection...
Preprint
Aspergillus fumigatus is an opportunistic fungal pathogen that can cause life-threatening invasive lung infections in immunodeficient patients. The cellular and molecular processes of infection during onset, establishment and progression are highly complex and depend on both fungal attributes and the immune status of the host. Therefore, preclinica...
Article
Acute graft versus host disease (aGVHD) remains a major complication in patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT), the only curative treatment for many malignant hematologic diseases. After initial priming in secondary lymphoid organs, alloreactive donor T cells efficiently migrate to the intestinal tract, l...
Article
Blood platelets play key roles in hemostasis and thrombosis and are the second most abundant cell type in the circulation. Due to their short life span of only a few days, anuclear platelets are continuously replenished and thus provide a classic system to study hematopoiesis. In mammals, platelets are produced by megakaryocytes (MKs) that are pred...
Article
Full-text available
The thymus is not only extremely sensitive to damage but also has a remarkable ability to repair itself. However, the mechanisms underlying this endogenous regeneration remain poorly understood, and this capacity diminishes considerably with age. We show that thymic endothelial cells (ECs) comprise a critical pathway of regeneration via their produ...
Article
Full-text available
In mammals, megakaryocytes (MKs) in the bone marrow (BM) produce blood platelets, required for hemostasis and thrombosis. MKs originate from hematopoietic stem cells and are thought to migrate from an endosteal niche towards the vascular sinusoids during their maturation. Through imaging of MKs in the intact BM, here we show that MKs can be found w...
Article
SLAMF7 is under intense investigation as a target for immunotherapy in multiple myeloma. In this study, we redirected the specificity of T cells to SLAMF7 through expression of a chimeric antigen receptor (CAR) derived from the huLuc63 antibody (elotuzumab) and demonstrate that SLAMF7-CAR T cells prepared from patients and healthy donors confer pot...
Article
Full-text available
Donor CD4+Foxp3+ regulatory T cells (T reg cells) suppress graft-versus-host disease (GvHD) after allogeneic hematopoietic stem cell transplantation (HCT [allo-HCT]). Current clinical study protocols rely on the ex vivo expansion of donor T reg cells and their infusion in high numbers. In this study, we present a novel strategy for inhibiting GvHD...
Article
Endogenous thymic regeneration is a crucial function that allows for renewal of immune competence following immunodepletion caused by cytoreductive chemotherapy or radiation; however, the mechanisms governing this regeneration remain poorly understood. Moreover, despite this capacity, prolonged T cell deficiency is a major clinical hurdle in recipi...
Article
Adoptive transfer of donor-derived cytolytic T-lymphocytes (CTL) has evolved as a promising strategy to improve graft-versus-leukemia (GvL) effects in allogeneic hematopoietic stem-cell transplantation. However, durable clinical responses are often hampered by limited capability of transferred T cells to establish effective and sustained antitumor...
Article
Full-text available
West African torrent-frogs of the genus Odontobatrachus currently belong to a single species: Odontobatrachus natator (Boulenger, 1905). Recently, molecular results and biogeographic separation led to the recognition of five Operational Taxonomic Units (OTUs) thus identifying a species-complex. Based on these insights, morphological analyses on mor...
Conference Paper
A young tomographic imaging method is growing up: since the first publication of magnetic particle imaging (MPI) [1] different hardware designs and scanner concepts have been shown [2], furthermore multiple studies on optimizing magnetic tracer materials have been published [3]. The next step on the way to the clinical routine is the pre-clinical t...
Article
Donor CD4+Foxp3+ regulatory T cells (Tregs) suppress graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HCT) while maintaining the anti-tumoral effect of transplanted conventional T cells in preclinical mouse models. Current clinical study protocols with donor Tregs for treatment or prophylaxis of GVHD r...
Article
Thymopoiesis is a complex process involving crosstalk between developing thymocytes and the non-hematopoietic stromal microenvironment, which includes thymic epithelial cells (TECs), fibroblasts and endothelial cells (ECs). Despite its importance, the thymus is exquisitely sensitive to cellular insults, including cytoreductive chemo- and radiation...
Article
Full-text available
Whereas interspecific associations receive considerable attention in evolutionary, behavioural and ecological literature, the proximate bases for these associations are usually unknown. This in particular applies to associations between vertebrates with invertebrates. The West-African savanna frog Phrynomantis microps lives in the underground nest...
Article
Full-text available
Multiple activities are ascribed to the cytokine tumor necrosis factor (TNF) in health and disease. In particular, TNF was shown to affect carcinogenesis in multiple ways. This cytokine acts via the activation of two cell surface receptors, TNFR1, which is associated with inflammation, and TNFR2, which was shown to cause anti-inflammatory signaling...
Thesis
In acute graft-versus-host disease (GVHD) alloreactive donor T cells selectively damage skin, liver, and the gastrointestinal tract while other organs are rarely affected. The mechanism of this selective target tissue infiltration is not well understood. We investigated the importance of alloantigen expression for the selective organ manifestation...
Article
Full-text available
Background Acute graft-versus-host disease (aGVHD) poses a major limitation for broader therapeutic application of allogeneic hematopoietic cell transplantation (allo-HCT). Early diagnosis of aGVHD remains difficult and is based on clinical symptoms and histopathological evaluation of tissue biopsies. Thus, current aGVHD diagnosis is limited to pat...
Article
Tumor necrosis factor (TNF) signals via two cell surface receptors, TNFR1 and TNFR2 and is involved in a number of physiological processes including the regulation of immune cell homeostasis and function. Initially identified to cause tumor necrosis, a number of recent studies showed TNF to drive inflammation-induced cancer and metastasis. We emplo...
Article
The cytokine tumor necrosis factor (TNF) has pleiotropic functions both in normal physiology and disease. TNF signals by the virtue of two cell surface receptors TNFR1 and TNFR2. Exogenous TNF promotes experimental metastasis in some models, yet the underlying mechanisms are poorly understood. To study the contribution of host TNFR1 and TNFR2 on tu...
Article
Full-text available
Background: Multiple myeloma (MM) is a B-cell malignancy, where malignant plasma cells clonally expand in the bone marrow of older people, causing significant morbidity and mortality. Typical clinical symptoms include increased serum calcium levels, renal insufficiency, anemia, and bone lesions. With standard therapies, MM remains incurable; there...
Data
CD31 staining verifies MOPC-315 localization inside blood vessels in the lung. (A) Representative immunofluorescence staining of an IgA+CD4+ MOPC-315.BM cell within a pulmonary CD31+ vessel in the lung taken from untreated mice 33 days after MM injection. (B) Negative control staining, without anti-CD31, anti-IgA and anti-CD4. Only secondary strepa...
Data
Verification of in vivo signal localization by ex vivo BLI. BALB/c wild type mice were injected with 1×105 MOPC-315.BM luc+ cells via the tail vein. 19 days after inoculation tumors were established in all mice and readily detected by BLI. Then treatment was started ( = day 0 of treatment). Mice received 5 mg/kg melphalan or mock treatment (vehicle...
Data
In vivo BLI measurements strongly correlate with MM burden determined by flow cytometry in spleen and femur/tibia. To additionally verify that non-invasive BLI data correlate with actual MM load we measured in vivo BLI signals from the spleen and femur/tibia and subsequently extracted cells from both organs for FACS analysis. For FACS MM cells were...
Data
Flow cytometric measurement of surface receptors associated with BM homing and infiltration of myeloma cells. MOPC-315.BM luc+ myeloma cells were either directly taken from cell culture or extracted from BM and spleen as indicated and identified as CD138+CD4+ double positive cells. α4β7 integrin positive MOPC-315.BM luc+ cells were identified by fl...
Data
Representative flow cytometry gating scheme according to the fluorescence minus one method (FMO). (A) First, live cells were identified using propidium iodide staining. Within the live cells the gate for CD138+CD4+ MM cells was set and applied to all samples within the measurement. Among those cells the quadrant gate for α4 and α4β7 was set accordi...
Data
CD4 expression of MOPC-315.BM luc+ and the wild type (WT) cell line. MOPC-315.BM luc+ and MOPC-315 WT cells (obtained from ATCC) were stained for CD4. Only live cells as determined by propidium iodide staining were used for the analysis. Both cell lines clearly expressed CD4 to the same extent. Therefore, the constitutive CD138 and CD4 co-expressio...
Article
1809 Various imaging platforms are well established in hematology research. Nevertheless, the three-dimensional architecture of the bone marrow and tumor growth within this microenvironment remain largely uncharacterized. To date the major hindrance to microscopically image tumor engraftment and the immune response in the bone marrow on a single ce...
Article
Full-text available
Understanding the spatiotemporal changes of cellular and molecular events within an organism is crucial to elucidate the complex immune processes involved in infections, autoimmune disorders, transplantation, and neoplastic transformation and metastasis. Here we introduce a novel multicolor light sheet fluorescence microscopy (LSFM) approach for de...
Article
Full-text available
Astylosternus laticephalus sp. nov. Rödel, Hillers, Leaché, Kouamé, Ofori-Boateng, Diaz & Sandberger is described from eastern Ivory Coast and western and central Ghana, and compared to Astylosternus occidentalis Parker, 1931 from the western part of the Upper Guinea forest zone (western Ivory Coast, Liberia, Guinea and Sierra Leone). Based on a co...
Article
1110 The cytokine tumor necrosis factor-α (TNF) has pleiotropic functions both in normal physiology and disease. TNF and its relative lymphotoxin-α (LT) signal by activating two cell surface receptors TNFR1 and TNFR2. TNFR1 is expressed on most cells whereas TNFR2 is mainly expressed in cells of the hematopoietic system. TNF-TNFR interactions were...
Article
3746 Acute graft-versus-host disease (aGvHD) is an immune syndrome after allogeneic hematopoietic cell transplantation (allo-HCT) caused by alloreactive donor T cells that attack the gastrointestinal tract, liver and skin. Thus, early T cell migration patterns to these organs could provide first cues for the onset of aGvHD. Hence, a unique surface...
Article
Full-text available
We report on the search and rediscovery of the Liberian Nimba toad, Nimbaphrynoides liberiensis, 30 years after its original description. A small surviving population could be traced in the surroundings of the type locality Mount Alpha, Liberia. The type locality was meanwhile destroyed by open cast mining. Similar to the Guinean Nimba toad, Nimbap...
Article
2436 Poster Board II-413 Sensitive in vivo imaging methods have advanced the fields of stem cell transplantation, graft-versus–host disease (GVHD) and graft-versus-tumor responses (GVT). Near-infrared (NIF) fluorescent proteins (FP) appear advantageous for deeper tissue penetration due to minimized absorbance by hemoglobin, water and lipids. Theref...
Article
4497 Patients undergoing allogeneic hematopoietic cell transplantation (allo-HCT) are at high risk to develop acute graft-versus-host disease (aGVHD) which is caused by alloreactive donor T cells. Early diagnosis of aGVHD remains difficult: there are no efficient methods to identify patients at risk of aGVHD, which could improve disease prevention....
Article
3559 Poster Board III-496 Acute graft-versus-host disease (GVHD) can be spatio-temporally separated in an initiation phase confined to secondary lymphoid organs (SLO) followed by the GVHD effector phase in the intestinal tract (GIT), liver and skin. Employing non-invasive bioluminescence imaging, fluorescence microscopy and flow cytometry in a muri...
Article
61 In vivo models of complex immune processes like stem cell engraftment, host-pathogen interactions or anti-tumor responses face the challenge to either provide dynamic information in low resolution (e.g. in vivo bioluminescence Imaging, BLI) or provide high resolution information with a limited field of view (e.g. multi-photon laser scanning micr...