Christa Ringers

Christa Ringers
Uppsala University | UU · Department of Pharmaceutical Biosciences

Doctor of Medicine
Automating phenotypic screening in 3D cell cultures.

About

18
Publications
1,361
Reads
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198
Citations
Introduction
My current project aims to optimize phenotypic screening in 3D to support precision medicine in colorectal cancer treatment. Specifically, I want to investigate whether morphological profiling can identify potential resistance mechanisms and variability between patients. Hence, my main research interests are phenotypic screening, morphological profiling, 3D cell culture, precision medicine, and the biology behind drug resistance and patient variability.
Additional affiliations
January 2017 - February 2019
Norwegian University of Science and Technology
Position
  • PhD Student
October 2014 - December 2014
University of Amsterdam
Position
  • Teaching assistent
Description
  • Teaching assistant in a laboratory course (Genetics and Evolution).
Education
August 2015 - June 2017
September 2013 - December 2013
University of British Columbia - Vancouver
Field of study
  • Computer science, philosophy, cognitive science
September 2011 - August 2014
University of Amsterdam
Field of study
  • Psychobiology

Publications

Publications (18)
Article
Motile cilia are actively beating hair-like structures that cover the surface of multiple epithelia. The flow that ciliary beating generates is utilized for diverse functions and depends on the spatial location and biophysical properties of cilia. Here we show that the motile cilia in the nose of aquatic vertebrates are spatially organized and stab...
Article
Full-text available
Motile cilia are miniature, propeller-like extensions, emanating from many cell types across the body. Their coordinated beating generates a directional fluid flow, which is essential for various biological processes, from respiration to reproduction. In the nervous system, ependymal cells extend their motile cilia into the brain ventricles and con...
Article
Motile cilia are miniature, whip-like organelles whose beating generates a directional fluid flow. The flow generated by ciliated epithelia is a subject of great interest, as defective ciliary motility results in severe human diseases called motile ciliopathies. Despite the abundance of motile cilia in diverse organs including the nervous system, t...
Article
Two new studies elegantly identify a missing link between idiopathic scoliosis and the Reissner fiber.
Article
Full-text available
Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles...
Preprint
Full-text available
Motile cilia are hair-like cell extensions present in multiple organs of the body. How cilia coordinate their regular beat in multiciliated epithelia to efficiently displace fluids remains elusive. Here, we propose the zebrafish nose as an accessible model system to study ciliary dynamics, due to its conserved properties with other ciliated tissues...
Preprint
Full-text available
Motile cilia defects impair cerebrospinal fluid (CSF) flow, and can cause brain and spine disorders. To date, the development of ciliated cells, their impact on CSF flow and their function in brain and axial morphogenesis are not fully understood. Here, we have characterized motile ciliated cells within the zebrafish brain ventricles. We show that...
Article
Full-text available
The developing brain undergoes drastic alterations. Here, we investigated developmental changes in the habenula, a brain region that mediates behavioral flexibility during learning, social interactions, and aversive experiences. We showed that developing habenular circuits exhibit multiple alterations that lead to an increase in the structural and...
Data
Video S5. The CSF Flow Is Contributed by a Directional, Near-Wall Flow and a Pulsatile Flow, Related to Figure 4 Interfering with the heartbeat (upon heart microdissection) or ciliary beating (upon mutation of elipsa, smh and foxj1a) affects the CSF flow dynamics at 2 dpf, as shown by confocal imaging along the sagittal midline of the diencephalic...
Data
Video S1. Analysis of T2BGSZ10 (foxj1b:gfp) and 0.6kbfoxj1a:gfp Transgenic Lines, Related to Figure 1 The ventricular system is composed of three interconnected cavities: the telencephalic ventricle, the diencephalic ventricle, and the rhombencephalic ventricle. The GFP positive cells in both the T2BGSZ10 (foxj1b:gfp) and Tg(foxj1a:gfp)nw6Tg trans...
Data
Video S4. The CSF Flow Patterns of 2- and 4-dpf Larval Zebrafish, Related to Figure 4 The CSF flow of 2 and 4 dpf larval zebrafish is contributed by two main components; a directional, near-wall flow and a pulsatile flow, as shown by confocal imaging along the sagittal midline of the diencephalic ventricle upon ventricular co-injection of 70 kDa R...
Data
Video S7. Upon Bodily Movement, the Stringent Compartmentalization of the 2-dpf Larval Ventricular System Is Temporarily Disrupted, Related to Figure 6 The baseline cilia-mediated, unidirectional flow along the diencephalic ventricular walls of zebrafish larvae compartmentalizes the CSF to the separate ventricles, with little interventricular exch...
Data
Video S3. Motile Cilia Point toward an Anterior Direction and Beat with a Rotational Movement, Related to Figure 2 Light-sheet recording (frame rate: 400 Hz) along the dorsal wall of the diencephalic ventricle. Anterior to the left, posterior to the right. The analysis of this recording is shown in Figure 2B.
Data
Video S2. Cilia Located in the Dorsal and Ventral Walls of the Diencephalic Ventricle Are Motile, Related to Figure 2 Light-sheet recording (frame rate: 100 Hz) of the sagittal midline of a 2 dpf β-actin:arl13b-gfp transgenic larvae. The analysis of this recording is shown in Figure 2A.
Data
Video S6. 3D Analysis of CSF Flow Patterns in the Telencephalic and Diencephalic Ventricles, Related to Figure 4 CSF flow patterns and pulsations in telencephalic ventricle (TV) and diencephalic ventricle (DV) were obtained by confocal microscopy upon ventricular injection of fluorescent particles (Φ = 160 nm) at 2 dpf. Sequential recordings were...

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