Chris Mathes

Chris Mathes
AnaBios

Ph.D.

About

27
Publications
3,003
Reads
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789
Citations
Citations since 2016
1 Research Item
94 Citations
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2016201720182019202020212022051015
Introduction
Leader of the Business Development team that interfaces between the scientists of AnaBios and the partner scientists of biotech and pharma companies. Responsible for all sales and marketing activities and contributing to the corporate strategy of the company.
Additional affiliations
June 1996 - July 1997
Max Planck Institute for Biophysical Chemistry
Position
  • PostDoc Position
Description
  • Role of the sphingomyelin pathway in regulation of Icrac
June 1995 - June 1996
Stanford University
Position
  • PostDoc Position
Description
  • Inactviation of K channels in squid giant fiber lobe neurons.
January 1991 - June 1995
Education
September 1988 - March 1995
University of California, Los Angeles
Field of study
  • Neuroscience
September 1984 - June 1988
Stanford University
Field of study
  • Biological Sciences

Publications

Publications (27)
Article
One of the significant challenges for automated patch clamp has been to incorporate amplifiers with the ability of adjusting to changes in membrane capacitance, series resistance (Rs) and voltage-clamp time constants without user intervention. If these adjustments are not made correctly there is a risk of generating un-precise data or even losing d...
Article
Full-text available
The conventional patch clamp has long been considered the best approach for studying ion channel function and pharmacology. However, its low throughput has been a major hurdle to overcome for ion channel drug discovery. The recent emergence of higher throughput, automated patch clamp technology begins to break this bottleneck by providing medicinal...
Article
Full-text available
Alpha7 nicotinic acetylcholine receptor channels are important ligand-gated ion channels that are fast desensitizing, cation selective and have been implicated in the pathophysiology of schizophrenia and Alzheimer's disease. We report here high quality alpha7 parallel patch clamp recordings using the QPatch automated patch clamp system. The QPatch...
Article
Full-text available
The suitability of an automated patch clamp for the characterization and pharmacological screening of calcium release-activated calcium (CRAC) channels endogenously expressed in RBL-2H3 cells was explored with the QPatch system. CRAC currents (I( CRAC)) are small, and thus precise recordings require high signal-to-noise ratios obtained by high seal...
Article
The 2nd Annual Ion Channel Targets (ICT) Conference (by Select Bioscience LLC) was held in Boston on 12-13 September 2006. A healthy mixture of scientists from pharma, biotech and academic sectors attended the meeting. The speaker list reflected this mixture. In general, the conference focused on new ion channel targets and the methods for studying...
Article
Full-text available
The QPatch 16 significantly increases throughput for gigaseal patch clamp experiments, making direct measurements in ion channel drug discovery and safety testing feasible. Released to the market in the Autumn of 2004 by Sophion Bioscience, the QPatch originated from work done at NeuroSearch (Denmark) in the early days of automated patch clamp. Tod...
Article
Full-text available
The authors used the PatchXpress 7000A system to measure compound activity at the hERG channel using procedures that mimicked the "gold-standard" conventional whole-cell patch clamp. A set of 70 compounds, including hERG antagonists with potencies spanning 3 orders of magnitude, were tested on hERG302-HEK cells using protocols aimed at either ident...
Article
Full-text available
Large quaternary ammonium (QA) ions block voltage-gated K(+) (Kv) channels by binding with a 1:1 stoichiometry in an aqueous cavity that is exposed to the cytoplasm only when channels are open. S-nitrosodithiothreitol (SNDTT; ONSCH(2)CH(OH)CH(OH)CH(2)SNO) produces qualitatively similar "open-channel block" in Kv channels despite a radically differe...
Chapter
Signal transduction events mediate and regulate secretion in specialized cells, including mast cells. Secretion occurs when vesicles or granules containing molecular transmitters fuse with a specific region of the plasma membrane and release their contents into the cleft near an adjacent cell or into the extracellular milieu (i.e., bloodstream). Th...
Article
Full-text available
Whole cell patch-clamp recordings were made to study the regulation of the store-operated calcium release-activated calcium current (ICRAC) by metabolites involved in the sphingomyelin pathway in RBL-2H3 cells. Sphingosine, a regulator of cell growth, inhibits ICRAC completely within 200 s and independently from conversion to either sphingosine 1-p...
Article
Full-text available
Inactivation of delayed rectifier K conductance (gk) was studied in squid giant axons and in the somata of giant fiber lobe (GFL) neurons. Axon measurements were made with an axial wire voltage clamp by pulsing to VK (approximately -10 mV in 50-70 mM external K) for a variable time and then assaying available gK with a strong, brief test pulse. GFL...
Article
Full-text available
Inward currents activated by 8-bromc-cGMP and by muscarinic agonist were compared in N1E-115 mouse neuroblastoma cells using perforated-patch voltage clamp and Fura-2 imaging. The cGMP analog activates a voltage-independent inward current that is carried at least in part by Ca2+ because it persists in Na(+)-free saline when Ca2+ is present and is b...
Article
Full-text available
The relationship between the depletion of IP3-releasable intracellular Ca2+ stores and the activation of Ca(2+)-selective membrane current was determined during the stimulation of M1 muscarinic receptors in N1E-115 neuroblastoma cells. External Ca2+ is required for refilling Ca2+ stores and the voltage-independent, receptor-regulated Ca2+ current r...
Article
Muscarinic agonists elicit large increases in intracellular Ca2+ and guanosine 3',5'-cyclic monophosphate (cGMP) in N1E-115 neuroblastoma cells. Both signals are blocked in cells loaded with the Ca2+ buffer 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid showing that the increase in intracellular Ca2+ concentration ([Ca2+]i) is necessary t...
Article
Full-text available
The activation of muscarinic receptors in N1E-115 neuroblastoma cells elicits a voltage-independent calcium current. The current turns on slowly, reaches its maximum value approximately 45 s after applying the agonist, is sustained as long as agonist is present, and recovers by one half in approximately 10 s after washing the agonist away. The curr...
Article
Full-text available
The effects of calphostin A on cytoplasmic calcium levels, receptor-mediated calcium release, and membrane input resistance were measured in neuroblastoma cells. Calphostin A is a lipophilic, light-sensitive perylenequinone that generates singlet oxygen when illuminated. It inhibits the activity of protein kinase C (IC50 = 250 nM), but only in the...
Article
Full-text available
We modified existing acetylcholinesterase (AChE) histochemical techniques to visualize cholinergic neuronal somata and processes in the same rat brain. The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualize...
Article
Full-text available
Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtypes(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (+/-)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP greater than pirenzepine greater...
Article
Full-text available
The macroscopic and single-channel properties of sodium currents and membrane potential were studied in intact extensor digitorum longus (EDL) muscle fibers from mdx (C57BL/10ScSn-mdx) and normal (C57BL/10SnJ) mice. The voltage dependence of activation and inactivation were determined and the associated gating charges were calculated to determine i...
Article
Buspirone was studied to determine whether the detailed profile of male sexual behavior observed following treatment with the prototypical 5-HT1A ligand, 8-OH-DPAT, can be generalized to other 5-HT1A agonist drugs. Systemic and intrathecal (IT) routes of administration were compared. Like DPAT, significant reduction in intromission frequency follow...
Article
Full-text available
A B S TRACT The relationship between the depletion of IP3-releasable intracellu- lar Ca z+ stores and the activation of Ca2+-selective membrane current was deter- mined during the stimulation of M1 muscarinic receptors in N1E-115 neuroblas- toma cells. External Ca 2+ is required for refilling Ca 2+ stores and the voltage-inde- pendent, receptor-reg...
Article
Thesis (Ph. D.)--University of California, Los Angeles, 1995. Typescript (photocopy). Vita. Includes bibliographical references.

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