Chris Harrington

Chris Harrington
Royal Surrey County NHS Foundation Trust | RSCH · Department of Clinical Biochemistry

MSc PhD FRSC FRCPath

About

120
Publications
28,764
Reads
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Citations
Introduction
The Supra-Regional Assay Service, SAS, Trace Element Lab at Guildford measures: Cu, Zn and Se for nutritional purposes; As, Pb and Cd for toxicological profiling; and Co, Cr and Ti in hip-replacement patients. An active research program looking at: trace elements in human nails; Co, Cr and Ti in serum, blood, hip-aspirates; and human metallomics. We run an ISO 17043 accredited External Quality Assessment scheme for trace elements. Details can be found here: http://www.surreyeqas.org.uk
Additional affiliations
September 2007 - January 2009
Nottingham Trent University
Position
  • Professor (Associate)
January 2003 - January 2007
University of Leicester
Position
  • Senior Researcher
February 1999 - January 2003
De Montfort University
Position
  • Lecturer then Senior Lecturer
Education
October 2010 - June 2012
University College London
Field of study
  • Clinical Biochemistry
October 1985 - June 1988
University of South Wales
Field of study
  • Combined Science, chemistry and environmental pollution

Publications

Publications (120)
Article
The production and evaluation of an isotopically enriched metalloprotein standard for use as a calibrant in species-specific isotope dilution analysis by HPLC coupled to inductively coupled plasma mass spectrometry is described. Using a model system involving the copper-containing protein rusticyanin (Rc) from the bacterium Acido-thiobacillus ferro...
Article
Full-text available
Hip replacements are used to improve the quality of life of people with orthopaedic conditions, but the use of metal-on-metal (MoM) athroplasty has led to poor outcomes for some patients. These problems are related to the generation of micro to nano sized metal wear particles containing Cr, Co or other elements, but the current analytical methods u...
Article
Full-text available
Background Patients with metal-on-metal hip replacements require testing for cobalt and chromium. There may also be a need to test for titanium, which is used in the construction of the femoral stem in total hip replacements. It is not possible to use quadrupole inductively coupled plasma mass spectrometry due to interferences. Methods Titanium wa...
Poster
Full-text available
Purpose: To present the capabilities and advantages of triple quadrupole ICP-MS for clinical research analysis. To illustrate how accurate and precise results can be obtained for Se in the presence of Gd in clinical research specimens. To show how triple quadrupole technology provides improved phosphorus and sulphur data.
Article
Full-text available
Objectives: We investigated the reliability of the cobalt-chromium (CoCr) synovial joint fluid ratio (JFR) in identifying the presence of a severe aseptic lymphocyte-dominated vasculitis-associated lesion (ALVAL) response and/or suboptimal taper performance (SOTP) following metal-on-metal (MoM) hip arthroplasty. We then examined the possibility th...
Article
This review covers advances in elemental speciation by a range of coupled techniques, including HPLC-ICP-MS, HPLC-HG-AFS and GC-ICP-MS.
Article
This is the 13th Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2019. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states, organometallic compounds, coordination...
Article
Aim: This report aims to render a proposed concept model for cancer risk in Nigerian electronic waste exposure by making deductions from data on the assessment of Nigerians' exposure to toxic metals in e-waste, using biomarkers of exposure and genotoxicity to evaluate the risk of cancer development. Material and methods: In the cross-sectional stud...
Article
This is the 12th Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2018. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordination...
Article
Biomedical analytical methods often rely on indirect measurements, such as immunoassays, which can lack effective metrological traceability. In the nephelometric determination of ceruloplasmin (Cp), an important protein whose circulating level is altered in Wilson's disease (WD), the anti-Cp antibody used is not specific for the biologically active...
Article
This is the 11th Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2017. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordination...
Presentation
Full-text available
Presentation to WDA USA on new Cu methods for the diagnosis and monitoring of Wilson Disease a rare inherited disease of copper metabolism.
Article
The determination of copper and iron in liver biopsies have important roles in the diagnosis of Wilson’s Disease and haemochromatosis respectively. An external quality assurance scheme is essential for analytical validation of results, however, none was available for these analytes at the time of this investigation. Accordingly a pilot scheme was e...
Research
ABSTRACT The determination of copper and iron in liver biopsies have important roles in the diagnosis of Wilson’s Disease and haemochromatosis respectively. An external quality assurance scheme is essential for analytical validation of results, however, none was available for these analytes at the time of this investigation. Accordingly a pilot sch...
Article
Exposure to titanium (Ti), via the ingestion of pigment grade Ti dioxide (TiO2), is commonplace for Westernised populations. It may also occur as a consequence of metal ion leaching in...
Poster
Full-text available
Results from an EQA solid sample scheme for the analysis of Cu and Fe in liver biopsy samples. Liver biopsies are used to diagnose Wilson's disease (Cu) and genetic haemochromatosis (Fe).
Article
This is the tenth Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2016. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordinatio...
Presentation
Full-text available
Current ICP-MS practice for trace elemental analysis in clinical science. Covering instrumentation, interferences, clinical applications, HPLC-ICP-MS, S and P analysis by triple quadrupole instrumentation, new application areas.
Article
This is the ninth Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2015. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordinatio...
Presentation
Full-text available
Presentation showing the performance of the iCap triple quadrupole ICP-MS instrument compared to the iCap-Q for trace elements in clinical samples.
Article
This is the eighth Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2014. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordinati...
Presentation
Full-text available
Talk given at the 2016 Thermo ICP-OES Users Group Meeting held at Jodrell Bank, UK. The presentation covers the clinical significance of taper wear in metal-on-metal replacements, which causes the production of Ti; development of a method based on ICP-OES; comparison to ICP-MS/MS; and the results for approximately 500 patient serum samples.
Conference Paper
Full-text available
EQA is the cornerstone of quality assurance and method validation in clinical testing laboratories in the UK, ensuring that the results of patient investigations are reliable and comparable wherever they are produced. In this talk we focus specifically on EQA (ISO 17043:2010) for laboratories performing trace element measurements, although many of...
Conference Paper
Full-text available
Inductively Coupled Plasma Mass Spectrometry: Bring a Little Sunshine into Your Laboratory Chris F. Harrington, Deputy Director, SAS Trace Element Laboratory, 15 Frederick Sanger Road, Surrey Research Park, Guildford, GU2 7YD. Abstract Many hospitals have a requirement for the determination of trace elements, for: nutritional purposes (Cu, Zn, Se...
Article
Full-text available
External Quality Assessment (EQA) is the cornerstone of quality assurance and method validation in clinical testing labs in the UK, ensuring that the results of patient investigations are reliable and comparable wherever they are produced. In this article we focus specifi cally on EQA for laboratories performing trace element measurements, although...
Conference Paper
Full-text available
Trace Elements External Quality Assessment Scheme (TEQAS) provides specimens prepared using serum, whole blood, urine or dialysis fluids and water as the base material, spiked with various concentrations of up to 14 trace elements covering clinically relevant ranges. Also included is a solid matrix scheme, primarily to challenge measurements of Cu...
Conference Paper
Full-text available
Hip replacement devices • Clinical presentation. • Reaction of the regulator and EQA requirements. • Interpretation of blood Co and Cr concentrations. • Titanium method development by ICP-OES. • Ti levels in serum, blood and hip-aspirates. • Co, Cr and Ti in human samples. • Binding of Co and Cr to serum proteins by HPLC-ICP-MS. 3 Hip Replacement D...
Data
Full-text available
Annual Report: 2013-14 4th July 2014 EQA@S External Quality Assessment at Surrey
Article
Full-text available
This is the sixth Atomic Spectrometry Update (ASU) to focus specifically on advances in elemental speciation and covers a period of approximately 12 months from December 2012. This review deals with all aspects of the analytical speciation methods developed for: the determination of oxidation states; organometallic compounds; coordination compounds...
Conference Paper
Full-text available
Hip replacement devices Clinical presentation. Reaction of the regulator and EQA requirements. Interpretation of blood Co and Cr concentrations. Titanium method development by ICP-OES. Ti levels in serum, blood and hip-aspirates. Co, Cr and Ti in human samples. Binding of Co and Cr to serum proteins by HPLC-ICP-MS.
Article
Full-text available
Measurement of selenium in serum is an important clinical biomarker of nutritional status. The presence of gadolinium (Gd) in samples following administration of the contrast agents used for magnetic resonance imaging (MRI) results in a significant positive bias when using quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS). Three in...
Article
The relationship between arsenic (As) and selenium (Se) in the human body is poorly understood. We have investi-gated the concentrations of urinary As and Se in three ethnic groups (n = 63) in the United Kingdom and show that there is a positive correlation (r = 0.62, p < 0.001) between total concentrations of As and Se and that the ratio of these...
Article
Full-text available
In the Panasqueira mine area of central Portugal, some environmental media show higher metal(loid) concentrations when compared with the local geochemical background and the values proposed in the literature for these environmental media. In order to evaluate the effect of the external contamination on selected indexes of internal dose, As, Cd, Cu,...
Article
Full-text available
Mining activities may affect the health of miners and communities living near mining sites, and these health effects may persist even when the mine is abandoned. During mining processes various toxic wastes are produced and released into the surrounding environment, resulting in contamination of air, drinking water, rivers, plants, and soils. In a...
Article
Full-text available
In 2010 the Medicines and Healthcare Products Regulatory Agency (MHRA) issued a medical device alert recommending measurement of cobalt and chromium concentrations in blood from patients experiencing discomfort or pain associated with metal-on-metal hip prostheses. 1 2 This followed concerns relating to possible adverse effects of metal released fr...
Article
Full-text available
This is the fifth Atomic Spectrometry Update (ASU) to focus specifically on developments in elemental speciation and covers a period of approximately 12 months from January 2012. The International Union for Pure and Applied Chemistry (IUPAC) have evaluated speciation and provided a definition as follows: "speciation analysis is the analytical activ...
Article
The effect of zinc, cadmium, and copper on the growth, tolerance indices, and metal uptake of Merlin Festuca rubra from Trelogan mine, Wales, and Cascade Festuca rubra, a normal grass, were investigated by growth experiments in metal amended nutrient solution. The effect of zinc or copper on the malic acid concentration in both strains was also inv...
Article
Full-text available
Selenium is an important clinical biomarker of nutritional status; however, the occurrence of gadolinium in a patient's serum as a result of the contrast agents used during magnetic resonance imaging investigations, results in a significant positive bias in its measurement by inductively coupled plasma-quadrupole mass spectrometry.
Article
This review of the use of high-performance liquid chromatography (HPLC) for the speciation of organotin compounds which are primarily of significance in the marine environment, is divided into sections on the basis of the different HPLC modes of separation. However, it should be noted that such a classification does not exist in reality. For instan...
Article
Full-text available
Members of the Atomic Spectrometry Updates Editorial Board take a look back over 25 years at how the subject of atomic spectrometry has changed, and how the review articles have developed to reflect this.
Article
Platinum-containing drugs are widely used to treat cancer in a variety of clinical settings. Their mode of action involves the formation of DNA adducts, which facilitate apoptosis in cancer cells. Cisplatin binds to the N7 position of the purine DNA bases forming intrastrand cross-links between either two adjacent guanines [cis-Pt(NH(3))(2)d(pGpG),...
Article
Full-text available
Measurement of the different physicochemical forms of metals and metalloids is a necessary pre-requisite for the detailed understanding of an element's interaction with environmental and biological systems. Such chemical speciation data is important in a range of areas, including toxicology, ecotoxicology, biogeochemistry, food safety and nutrition...
Article
Earthworms native to the former mine site of Devon Great Consols (DGC), UK reside in soils highly contaminated with arsenic (As). These earthworms are considered to have developed a resistance to As toxicity. The mechanisms underlying this resistance however, remain unclear. In the present study, non-resistant, commercially sourced Lumbricus terres...
Article
Full-text available
A significant proportion of human cancers overexpress DNA polymerase beta (Pol beta), the major DNA polymerase involved in base excision repair. The underlying mechanism and biological consequences of overexpression of this protein are unknown. We examined whether Pol beta, expressed at levels found in tumor cells, is involved in the repair of DNA...
Article
Full-text available
Two species of arsenic (As) resistant earthworm, Lumbricus rubellus and Dendrodrillus rubidus, their host soils and soil excretions (casts) were collected from 23 locations at a former As mine in Devon, UK. Total As concentrations, measured by ICP-MS, ranged from 255 to 13,080 mg kg(-1) in soils, 11 to 877 mg kg(-1) in earthworms and 284 to 4221 mg...
Article
Full-text available
A pilot study was conducted to determine the applicability of toenails as a biomarker of exposure to elevated environmental arsenic (As) levels. A total of 17 individuals were recruited for the pilot study: 8 residents living near to a former As mine, Devon, UK, forming the exposed group, plus 9 residents from Nottinghamshire, UK, with no anticipat...
Article
Full-text available
This is the first Atomic Spectrometry Update (ASU) to focus specifically on developments in elemental speciation and covers a period of 10 months from January 2008. Other ASU reviews1–5 detail aspects of the research in this area, but our aim is to bring all of the work on this subject together in a single review. This new development comes as a re...
Article
Full-text available
The relationship of the total arsenic content of a soil and its bioaccumulation by earthworms (Lumbricus rubellus and Dendrodrilus rubidus) to the arsenic fraction bioaccessible to humans, measured using an in vitro physiologically-based extraction test (PBET), was investigated. Soil and earthworm samples were collected at 24 sites at the former ar...
Article
Full-text available
The consumption of fish and shellfish is a major route of human exposure to arsenic (As), because they contain relatively large concentrations of organoarsenicals, in particular arsenobetaine (AB). AB is considered non-toxic because of its rapid excretion from the human body. However, previous studies on human metabolism and excretion of AB have us...
Article
Full-text available
The relationship between the total arsenic concentration and the chemical speciation of arsenic in two species of earthworm (Lumbricus rubellus and Dendrodrilus rubidus) in relation to the host soil, was investigated for 13 sites of varying arsenic content, including a background level garden soil and a former mine site at the Devon Great Consols,...
Article
Full-text available
Bradykinin and substance P have been derivatised with cyclic diethylenetriaminepentaacetic anhydride (cDTPA) and subsequently labelled with natural and isotopically enriched Eu(3+). This enabled the detection and relative quantitation of the peptides using element-selective detection by high-performance liquid chromatography inductively coupled pla...
Article
Cellular resistance, both intrinsic and acquired, poses a problem in the effectiveness of platinum-based chemotherapy. The cytotoxic activity of Pt-based chemotherapeutic agents is derived from their ability to react with cellular DNA. Oxaliplatin binds to the N7 position of the purine DNA bases, forming mainly intrastrand cross-links between eithe...
Article
The use of high-performance liquid chromatography (HPLC) coupled to inductively coupled plasma mass spectrometry (ICP-MS) for the determination of methylmercury (MeHg+) in fish tissue and hair samples is described. Analysis of these sample types is required when carrying out biomonitoring studies to determine human dietary exposure to this toxic me...