Ching-Hua Su

Ching-Hua Su
Taipei Medical University | TMU · College of Medicine

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86
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Introduction
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Publications

Publications (86)
Article
Full-text available
Taiwanofungus camphoratus (Tc) is a basidiomycete fungus that causes brown heart rot of the aromatic tree Cinnamomum kanehirae. The Tc fruiting bodies have been used to treat hangovers, abdominal pain, diarrhea, hypertension, and other diseases first by aboriginal Taiwanese and later by people in many countries.
Article
Composite dressing composed of Rhizochitosan and RegenplexTM to promote wound healing were assessed. Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6. Physicochemical characterizations of Rhizochitosan demonstrated that it conta...
Article
Full-text available
Candida albicans (C. albicans) is an opportunistic human pathogen responsible for approximately a half of clinical candidemia. The emerging Candida spp. with resistance to azoles is a major challenge in clinic, suggesting an urgent demand for new drugs and therapeutic strategies. Alpha–enolase (Eno1) is a multifunctional protein and represents an i...
Article
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Context: Taiwanofungus camphoratus is a parasitic mushroom found in the heartwood of Cinnamomum kanehirai and is used as a nutritional supplement. It has an anticancer action, both alone and synergistically with amphotericin B (AmB). Objective: The study intended to assess the efficacy of a T camphoratus ethanol extract (TCEE) combined with AmB...
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The incidence of liver cancer, the second leading cause of cancer-related deaths has increased over the past few decades. Although recent treatments such as sorafenib are promising in patients with advanced hepatocellular carcinoma (HCC), the response rates remain poor thereby warranting the identification of novel therapeutic agents against liver...
Article
As peri-implantitis is associated with biofilm development, the characteristics of titanium implants may influence biofilm formation, and thereby increase the risk for inflammation. The objective of this study was to evaluate the effect of titanium surface roughness induced by various debridement methods of peri-implants, such as the use of an ultr...
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Full-text available
Taiwanofungus camphoratus (synonym Antrodia camphorata ) is a widely used medicinal fungus in the folk medicine of Taiwan with several pharmacological features such as anti-inflammatory, liver protection, antihypertensive, and antioxidative activities. The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds includ...
Article
Full-text available
A mycelial mattress of Rhizopus stolonifer obtained from a liquid static culture was utilized for wound dressing and biomedical use. Following screening of mutants induced by UV radiation, F6, exhibiting delayed sporangium formation was selected because its sporangium maturation exhibited a 5-day delay without significant loss of mycelial weight co...
Article
Candida albicans is considered to be an obligate diploid fungus. Here, we describe an approach to isolate aneuploids or haploids induced by the short-term (12-16 h) exposure of diploid reference strains SC5314 and CAI4 to the most commonly used antifungal drug, fluconazole, followed by repeated single-cell separation among small morphologically dis...
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LUFFACHITIN obtained from the residue of the sponge-like dried fruit of Luffa aegyptiaca was developed as a weavable skin substitute in this study. A chemical analysis revealed that LUFFACHITIN was composed of a copolymer containing N-acetyl-glucosamine (~40%) as a major monomer with a filamentary structure as demonstrated by both optical and scann...
Article
Recently, the structure-function relationships between amphotericin B (AmB) and ergosterol have been solved using synthetic techniques that require a mycosamine-mediated direct binding interaction between AmB and ergosterol to form AmB ion channels. However, studies to directly probe the AmB-induced membrane permeability changes have not been condu...
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We have studied the micellization behavior of tertiary amine methacrylate-based diblock copolymers, comprising the hydrophobic poly[2-(diethylamino)ethyl methacrylate] (PDEA) and hydrophilic/polyelectrolyte poly[2-(dimethylamino)ethyl methacrylate] (PDMA) blocks. Revealed by small-angle X-ray scattering (SAXS) and dynamics light scattering, micelle...
Article
Ageing dynamically disrupts the multilayered supporting components of the skin that are held together by cell adhesion molecules (CAMs). Skin specimens from 33 female Chinese patients undergoing lower blepharoplasty were divided into three age groups and examined by haematoxylin and eosin (H&E) staining, immunohistochemistry (IHC) and Elastica-van...
Article
In this study, we analyzed the key parameters of modified transcutaneous lower blepharoplasty based on multidisciplinary principles (biochemical findings and biophysical wrinkling theory). A total of 408 female patients received our subciliary lower blepharoplasty between March 2002 and January 2010. The severity of the eyebags (dynamic wrinkle num...
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Candida albicans, the most prevalent human fungal pathogen, is considered to be an obligate diploid that carries recessive lethal mutations throughout the genome. Here we demonstrate that C. albicans has a viable haploid state that can be derived from diploid cells under in vitro and in vivo conditions, and that seems to arise through a concerted c...
Article
Full-text available
Candida albicans, the most prevalent human fungal pathogen, is considered to be an obligate diploid that carries recessive lethal mutations throughout the genome. Here we demonstrate that C. albicans has a viable haploid state that can be derived from diploid cells under in vitro and in vivo conditions, and that seems to arise through a concerted c...
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Niu-chang-chih, a medicinal polypore restricted to Taiwan on the endemic tree species Cinnamomum kanehirae, currently goes by the names Antrodia cinnamomea T.T. Chang & W.N. Chou, A. camphorata (M. Zang & C.H. Su) Sheng H. Wu & al., and Taiwanofungus camphoratus (M. Zang & C.H. Su) Sheng H. Wu & al., the latter two based on Ganoderma camphoratum M....
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The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, m...
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The use of multiple drugs in cancer therapy increases the efficacy of the potential therapeutic effects. In this study, the authors investigated the adjuvant effects of an ethanol extract of solid-state cultivated Taiwanofungus camphoratus (TCEE) and amphotericin B (AmB) in the human cancer cell lines RPMI7951 and MG63. Taiwanofungus camphoratus is...
Article
Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal age...
Article
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An ideal anticancer strategy should target only the malignant cells but spare the normal ones. In this regard, we established a platform, consisting of an antigen-delivering vehicle and a protein vaccine, for developing an immunotherapeutic approach with the potential for eliminating various cancer types. Mesenchymal stem cells (MSCs) have been dem...
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There is emerging interest in the use of standardized virally inactivated human platelet lysate preparations rich in GFs (growth factors) for cell cultures, cell therapy and clinical applications. In the present paper, we report a simple process to prepare a virally inactivated platelet lysate preparation rich in TGF-beta1 (transforming growth fact...
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Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined t...
Article
Far-infrared ray (FIR) radiation has been shown to be beneficial to human health; however, little scientific evidence of its mechanisms has been provided. In the present study, we investigated the effect of nonthermal-enhanced FIR on the expression of calmodulin (Cam) protein and nitric oxide (NO) production by RAW 264.7 macrophages. Results indica...
Article
Ganoderma lucidum (generic type), originally described from England, has been reported worldwide. In the Orient, an economically important fungus commonly known as 'ling-zhi' or 'chi-zhi' for more than 2000 years, has also been named G. lucidum. However, the identity of the Oriental fungus has been questioned in recent years. Earlier molecular stud...
Article
Structural evolution of syndiotactic polystyrene (sPS) in a cold crystallization process was quantitatively examined with in-situ small/wide-angle X-ray scattering and differential scanning calorimetry (SAXS/WAXS/DSC). After removal of background scattering from fractal-like matrix structure, SAXS profiles obtained during programmed heating of an a...
Article
Asthma is a chronic disease characterized by airway inflammation caused by the dysregulated production of cytokines secreted by allergen-specific type 2 T helper (Th2) cells. Antrodia camphorata is a commonly used fungus in Asian folk medicine, and A. camphorata polysaccharides are reported to possess anti-cancer activities. In this study, the immu...
Article
By means of time-resolved photoluminescence and photothermal techniques, after-effects from excited-state dynamics, energy migration, and conformational rearrangement of poly(9,9-di-n-octyl-2,7-fluorene) (PFO) and its homologues has been examined and interpreted with rotational potential maps from quantum mechanical calculations. Steady-state photo...
Article
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Determine the release of growth factors (GF) from platelet-rich fibrin (PRF) and supernatant serum to optimize clinical use. Platelet-derived growth factors-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were quantified in...
Article
The wound dressing of acrylic acid-grafted and gamma-polyglutamic acid/chitosan-immobilized polypropylene non-woven fabric (PP-AAg-PGCi) was produced in this study. Gamma-polyglutamic acid (gamma-PGA) was first applied in this field to composed with chitosan. The PP-AAg-PGCi wound dressings were expected to enhance the water absorbing, water permea...
Article
By means of in situ small/wide-angle X-ray scattering (SAXS/WAXS) and differential scanning calorimetry (DSC), we examined evolutions of lamellar crystal thickness for α and β crystals, respectively, in bulk-crystallized syndiotactic polystyrene (sPS) during the partial melting−reorganization process upon progressive heating up to 290 °C. For the S...
Article
Dermatophytes and Malassezia are common pathogen in Taiwan. However, systemic Candida albicans (C. albicans) infection in animals has not yet been reported here and is rare elsewhere in the world. This report, describe a young dog, in which a submandibular pyogranulomatous lymphadenitis caused by C. albicans infection progressed to systemic candidi...
Article
Ganoderma, known as Lingzhi or Reishi, has been traditionally administered throughout Asia for centuries as a cancer treatment and for other medicinal purposes. To investigate the inhibitory activity and explore the molecular mechanisms of anti-tumor effect on colorectal cancer cells in vitro and in vivo as well as to test the side effects of Ganod...
Article
By means of in situ small-angle X-ray scattering/wide-angle X-ray scattering/differential scanning calorimetry (SAXS/WAXS/DSC), structural evolution of poly(9,9-di-n-octyl-2,7-fluorene) (PFO) in a cold crystallization process was quantitatively examined. After removal of background scattering from the fractal-like structure, SAXS profiles obtained...
Article
Taiwanofungus camphoratus (T. camphoratus), a fungus and a Taiwan-specific, well-known traditional Chinese medicine, has long been used to treat diarrhea, hypertension, itchy skin, and liver cancer. To gain a large amount of T. camphoratus, several culture techniques have been developed, including solid-state culture and liquid-state fermentation....
Article
A durable sandwich wound dressing system with high liquid absorbing, biocompatibility, and antibacterial properties was designed. Various solution weight ratios of collagen to chitosan were used to immobilize on the polypropylene nonwoven fabric, which were pregrafted with acrylic acid (AA) or N-isopropyl acrylamide (NIPAAm) to construct a durable...
Article
To search for antimicrobial compounds against pathogenic bacteria from grape vines (Vitis spp.). To investigate the antimicrobial efficacy of active compounds towards methicillin-resistant Staphylococcus aureus (MRSA). The root extracts of taiwanese wild grape (Vitis thunbergii var. taiwaniana) showed marked activities against Gram-positive bacteri...
Article
Taiwanofungus camphoratus (syn. Antrodia camphorata), a medicinal mushroom in Taiwan, is reputed to provide several therapeutic benefits, but the wild fruiting body is very rare. In this study, we used Taiwanofungus camphoratus extracts from wild fruiting bodies and two types of artificial cultivation (solid-state culture and liquid-state fermentat...
Article
We have previously demonstrated that solution-cast films of conjugated polymers with grafted alkyl side chains comprise clearly identifiable domains ca. 10 nm in size upon solvent evaporation. Here we show that these nanodomains indeed serve as basic units for morphological development in poly(9,9-di-n-octyl-2,7-fluorene) (PFO) films during cold cr...
Article
Microbial control plays an important role in the clinical efficacy of dental practice. The purpose of this study was to develop a long-lasting antibacterial guided-tissue regeneration (GTR) barrier containing the non-shellfish chitosan from the fruiting body residue of Ganoderma tsugae. The powder of ground up G. tsugae fruiting body residue was pr...
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Antrodia camphorata (AC), a precious and unique folkloric medicinal mushroom enriched in polyphenolics, isoflavonoids, triterpenoids, and polysaccharides, has been diversely used in Formosa (Taiwan) since the 18th century. In this study, prostate cancer (PCa) cell lines PC-3 (androgen independent) and LNCaP (androgen responsive) were treated with A...
Article
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Wild and solid-state cultures (SSC) of Taiwanofungus camphoratus (aka Antrodia camphorata and Chang-chih [CC]) were sequentially extracted with cold water, methanol, and hot water to get cold-water-soluble (CWS), methanol-soluble (MS), and hot-water-soluble (HWS) extracts, respectively. Only the MS extract exhibited angiotensin-converting enzyme (A...
Article
The Antrodia camphorata crude extract (ACCE), an extract obtained from a precious traditional Chinese folkloric herbal medicine Zhan-Ku (a camphor tree mushroom) since the 18th century, has showed rather significant inhibitory effects on the growth and proliferation of the transitional cell carcinomas (TCC) cell lines RT4, TSGH-8301, and T24. On tr...
Article
Full-text available
Based on results of small-angle X-ray scattering and differential scanning calorimetric measurements, the equilibrium melting temperature and basal surface energy (σe) of crystalline poly(9,9-di-n-octyl-2,7-fluorene) (PFO) were preliminarily estimated as ca 451.6 K and 0.084 J/m2, respectively, via Gibbs–Thomson analysis. This σe value leads to a v...
Article
Human intervertebral disc (IVD) degeneration often initiated from the human nucleus pulposus (hNP) with aging leading to IVD destruction and extracellular matrix (ECM) depletion. Previously, we have successfully employed transforming growth factor-beta1 (TGF-beta1) to promote chondrogenesis of mesenchymal progenitor cells (MPCs) and immortalized hu...
Article
Bladder cancer has been cited to result from the neoplastic lesion with environmental and/or occupational factors identified as causatives. Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Most of the bladder cancer patients die from the invasive, metastatic TCC that has turned out to be resistant to chemotherapy. T24 ce...
Article
Here we report the phase behavior of poly(9,9-di-n-hexyl-2,7-fluorene) (PFH), which previously received little attention as compared to its homologues poly(9,9-di-n-octyl-2,7-fluorene) (PFO) and poly(9,9-di-(2'-ethylhexyl)-2,7-fluorene) (PFEH). By means of differential scanning calorimetry, X-ray diffraction, and electron microscopy, we show that t...
Article
Direct and non-intrusive observations of crystallization and melting behavior of and polymorphs in bulk syndiotactic polystyrene were made by means of temperature-programmed x-ray diffraction. Results indicated that the highest sustainable temperature identifiable via wide-angle x-ray diffraction using stepwise annealing at increasingly higher temp...
Article
SACCHACHITIN membranes, prepared from the waste residue of the fruiting body of Ganoderma taugae, were used in our previous study to enhance skin wound healing in animal models. In the present study, the effects of the membrane on the growth of keratinocytes and the activity of matrix metalloproteinases (MMPs), as well as on the healing of skin wou...
Article
By means of transmission electron microscopy (TEM) and X-ray diffraction (XRD) we demonstrate the presence of a previously unspecified modification (α‘) of the crystalline (α) phase in poly(9,9-di-n-octyl-2,7-fluorene) (PFO). The α‘ form is metastable and lower in melting temperature than the α form, and its formation is kinetically favored at temp...
Article
Full-text available
Direct and non-intrusive observations of crystallization and melting behavior of alpha and beta polymorphs in bulk syndiotactic polystyrene were made by means of temperature-programmed x-ray diffraction. Results indicated that the highest sustainable temperature identifiable via wide-angle x-ray diffraction using stepwise annealing at increasingly...
Article
SACCHACHITIN is a skin wound-healing membrane made of residual fruiting body of Ganoderma tsugae. Its effect on proliferating cell nuclear antigen (PCNA) expression in actively proliferating cells, type I collagen expression and tissue remodeling in the healing tissue, and the association of tissue-transglutaminase (t-TGase) with wound healing were...
Article
Structural evolution and its effect on the optical absorption/emission behavior of poly(2,5,2‘,5‘-tetrahexyloxy-8,7‘-dicyano-di-p-phenylenevinylene) (DH-CNPPV) upon short-term isothermal heat treatment at elevated temperatures were studied by means of a combination of X-ray diffraction, transmission electron microscopy, UV−vis spectroscopy, and pho...
Article
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Taiwanofungus Sheng H. Wu, Z.H. Yu, Y.C. Dai & C.H. Su is proposed as a new genus of lignicolous poly- pore, and Ganoderma comphoratum M. Zang & C.H. Su is designated as its generic type. New combinations for two species of Taiwanofungus are presented: T. camphoratus (M. Zang & C.H. Su) Sheng H. Wu, Z.H. Yu, Y.C. Dai & C.H. Su, and T. salmoneus (T....
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Antrodia camphorata (A. camphorata) is well-known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of A. camphorata extracts to protect against oxidative stress in vitro and against carbon tetrachloride (CCl(4))-induced hepatic injury in vivo. An extract of A. camphorata inhibited nonenzymatic iron-...
Article
In this case resport, we describe pyogranulomatous lymphadenitis due to Candida albicans that progressed to a systemic infection in a 10-kg, 1-year-old spayed female mixed-breed dog. The dog had bilaterally enlarged submandibular lymph nodes with peripheral oedema. Fine-needle aspirate of fluid from a lymph node and associated area was collected fo...
Article
Reported in this communication are our experimental findings that MEH-PPV is in fact mesomorphic in nature, with the tendency to form supramolecular assemblies with primitively layered structure (i.e., of smectic order) and yet maintaining its nematic-like optical texture upon heat treatment in the temperature range of 200 - 260 °C. Similarities in...
Article
The fruiting body and culture broth of many edible mushrooms contain water-soluble polysaccharides. Numerous researchers have reported that these polysaccharides have immunoenhancing effects. In this study, Hericium erinaceus and Hericium laciniatum were separately cultivated in a shaker at 25 degrees C for 25 days. Polysaccharides were extracted f...
Article
SACCHACHITIN membrane, a weavable skin substitute made from the residual fruiting body of Ganoderma tsugae, has been demonstrated to promote skin wound healing. Prior to its clinical application, it is critical to learn more about any possible cytotoxicity, immunogenicity, or allergy response, and at least some of its mechanism(s) of action(s). In...
Article
SACCHACHITIN membrane, a weavable skin substitute made from the residual fruiting body of Ganoderma tsugae, has been demonstrated to promote skin wound healing. Prior to its clinical application, it is critical to learn more about any possible cytotoxicity, immunogenicity, or allergy response, and at least some of its mechanism(s) of action(s). In...
Article
Full-text available
A high-performance liquid chromatographic (HPLC) analysis of triterpenoids from Ganoderma is developed and validated in an attempt to explore a way to differentiate a number of species of the genus Ganoderma. Results show that 64 samples examined in this study could be divided into 18 groups based on characteristics of the HPLC pattern of triterpen...
Article
In this study, Gram-positive Staphylococcus aureus lipoteichoic acid (LTA) dose dependently (0.1–1.0 μg/mL) and time dependently (10–60 min) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., thrombin and collagen). LTA also dose dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by coll...
Article
In this study, Escherichia coli LPS dose-dependently (100-500 microg/ml) and time-dependently (10-60 min) inhibited platelet aggregation in human and rabbit platelets stimulated by agonists. LPS also dose-dependently inhibited the intracellular Ca2+ mobilization in human platelets stimulated by collagen. In addition, LPS (200 and 500 microg/ml) sig...
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Full-text available
In this study, Sacchachitin membrane, prepared from the residue of the fruiting body of Ganoderma tsugae, was estimated for its effects on wound healing and the proliferation and migration of fibroblast cells. Two mirror-image wounds were made on the back of female guinea pigs by dissecting a 1.5×1.5 cm2 skin surface of full thickness. Sacchachitin...
Article
In this study, the mechanism involved in the antiplatelet activity of rutaecarpine in human platelet suspensions was investigated. In platelet suspensions (4.5×108/ml), rutaecarpine (100 and 200 μM) did not influence the binding of FITC-triflavin to platelet glycoprotein IIb/IIIa complex. Additionally, rutaecarpine (200 μM) did not significantly ch...
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For identification of genuine and counterfeit samples of Cordyceps sinensis, an RAPD (random amplified polymorphic DNA) analysis was performed. In the study, twelve samples were collected, of which six were genuine and six were counterfeit samples. Genuine samples of Cordyceps sinensis contain two parasitic larvae produced from each of the three pr...
Article
Triflavin, a 7.5-kD cysteine-rich polypeptide purified from Trimeresurus favoviridis snake venom, belongs to a family of Arg-Gly-Asp-(RGD)-containing peptides, termed disintegrins. In this study, aggregating human platelets dose-dependently induced vasoconstriction in de-endothelialized isolated rat thoracic aortas. At 5x10(7) cells per milliliter,...