Chiho Watanabe

Chiho Watanabe
Hiroshima University | HU · Graduate School of Integrated Sciences for Life

PhD

About

21
Publications
1,917
Reads
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107
Citations
Introduction
Chiho Watanabe currently works at Graduate School of Integrated Sciences for Life in Hiroshima University. She works on projects using in vitro cell models (artificial cells) such as polymer solutions, droplets (emulsions), and liposomes. Her aim is to understand life from materials and explore connections between them.
Additional affiliations
July 2020 - present
Hiroshima University
Position
  • Professor (Assistant)
Description
  • Working on interfacial phenomena, including liquid-liquid phase separation, droplets and liposomes.
January 2019 - June 2020
The University of Tokyo
Position
  • Professor (Assistant)
Description
  • Worked on projects of biomimetic system for basic understanding of life through (soft) matter.
April 2016 - December 2018
Tokyo University of Agriculture and Technology
Position
  • PostDoc Position
Description
  • Worked on a project on molecular diffusion in cell mimetic confinement.

Publications

Publications (21)
Article
Polymer micromaterials in a liquid or gel phase covered with a surfactant membrane are widely used materials in pharmaceuticals, cosmetics, and foods. In particular, cell-sized micromaterials of biopolymer solutions covered with a lipid membrane have been studied as artificial cells to understand cells from a physicochemical perspective. The charac...
Article
Full-text available
Tardigrades are able to tolerate almost complete dehydration by entering a reversible ametabolic state called anhydrobiosis and resume their animation upon rehydration. Dehydrated tardigrades are exceptionally stable and withstand various physical extremes. Although trehalose and late embryogenesis abundant (LEA) proteins have been extensively stud...
Article
Two droplets composed of different chemicals, 1-decanol and liquid paraffin, floating on the water surface show characteristic co-responsive behavior. The presence of two different types of droplets in the system imposes an asymmetry that would not be possible with single droplets alone. The self-propulsion and interactions between droplets appear...
Preprint
Biomolecular condensates driven by liquid-liquid phase separation (LLPS) have received attention as novel activity regulators of living organisms. In intracellular LLPS, an important question is what type of biomolecules form condensates under what conditions. In this regard, possible interactions between biomolecules have been investigated. Recent...
Article
Full-text available
The dextran–PEG system is one of the most famous systems exhibiting phase separation. Various phase behaviors, including the evaporation process of the dextran–PEG system, have been studied in order to understand the physicochemical mechanism of intracellular phase separation and the effect of condensation on the origin of life. However, there have...
Article
We investigated the effect of the adhered interface on the phase separation pattern using two or three adhered droplets containing a binary solution of poly(ethylene glycol) and gelatin. Under the experimental conditions, single domains of the gelatin-rich phase exhibited partial wetting to the droplet adhered interface (DAI) and nonadhered droplet...
Preprint
Full-text available
Tardigrades are able to tolerate almost complete dehydration by entering a reversible ametabolic state called anhydrobiosis and resume their animation upon rehydration. Dehydrated tardigrades are exceptionally stable and withstand various physical extremes. Although trehalose and late embryogenesis abundant (LEA) proteins have been extensively stud...
Chapter
Full-text available
Biomimetic lipid bilayer systems are a useful tool for modeling specific properties of cellular membranes in order to answer key questions about their structure and functions. This approach has prompted scientists from all over the world to create more and more sophisticated model systems in order to decipher the complex lateral and transverse orga...
Article
Membranes are ubiquitous structures in cells. The effects of membranes on various functional molecules have been reported, but their behaviors under macromolecular crowding and cell-sized confinement have not fully been understood. In this study, we model an intracellular environment by crowding micrometer-sized droplets and investigate the effects...
Article
Full-text available
Assembled water-in-oil droplets bounded by lipid bilayers are used in synthetic biology as minimal models of cell tissue. Microfluidic devices successfully generate monodispersed droplets and assemble them via droplet interface bilayesr (DIB) formation. However, a honeycomb pattern of DIB-bounded droplets, similar to epithelial tissues, remains unr...
Article
Membrane adhesion is a ubiquitous phenomenon in cells and is related to various biological events such as migration, morphogenesis, and differentiation. To understand the physicochemical aspects of membrane adhesion, liposome-liposome adhesion and liposome- substrate adhesion have been studied. Although membrane adhesion has been shown to increase...
Article
Molecular behaviors in small liquid droplets (pL scale), such as phase transitions and chemical reactions, are essential for the industrial application of small droplets and their use as artificial cells. However, the droplets often differ from those in bulk solutions (mL scale). Since the droplet size is much larger than the molecular size, the so...
Article
Liposomes have been attracted attention for cosmetic and pharmaceutical applications because of their high biocompatibility and biodegradability; however, even after long–term research, challenges still remain to develop their wider use. In this short review, we describe the basic issues and their possible solutions along with our recent achievemen...
Article
Micrometric membrane confinements and macromolecular crowding of cytoplasm are key factors that regulate molecular diffusion in live cells. Previous studies have shown that macromolecular crowding delays molecular diffusion. However, the effect of cell-size confinement on diffusion in the crowding environment is yet to be elucidated. Using fluoresc...
Article
A strong interplay between the neurodegerative effects of the amyloid β (Aβ) peptides and the cell lipid composition or metabolism has been evidenced in Alzheimer's disease. This appears to be, in part, related to Aβ-membrane interactions. Recently, an influence of the two cell fate-modulating single-chain sphingolipids, sphingosine (Sph) and sphin...
Article
An important question in membrane biological chemistry is whether bioactive signaling lipids act only as second messenger ligands or also through an effect on bilayer physical properties. Sphingosine (Sph) and sphingosine-1-phosphate (S1P) are single-chained charged sphingolipids that have antagonistic functions in the “sphingolipid rheostat” which...
Article
A recurring question in membrane biological chemistry is whether bioactive signaling lipids act only as second messenger ligands or also through an effect on bilayer physical properties. Sphingosine (Sph) and sphingosine-1-phosphate (S1P) are single-chained charged sphingolipids that have antagonistic functions in the "sphingolipid rheostat" which...
Article
Lipid rafts are assumed to undergo biologically important size-modulations from nanorafts to microrafts. Due to the complexity of cellular membranes, model systems become important tools, especially for the investigation of the factors affecting "raft-like" Lo domain size and the search for Lo nanodomains as precursors in Lo microdomain formation....

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