Chih-Hsin Lin

Taipei Medical University | TMU · College of Biomedical Engineering

Three-dimensional (3D) bioprinting, or additive manufacturing, is a rapid fabrication technique with the foremost objective of creating biomimetic tissue and organ replacements in hopes of restoring normal tissue function and structure. Generating the engineered organs with an infrastructure that is similar to that of the real organs can be benefic...

Drug metabolism leads to biotransformations of pharmaceutical substances that alter drug efficacy, toxicity, as well as drug interactions. Modeling these processes ex vivo stands to greatly accelerate our capacity to develop safe and efficacious drugs and formulations. Recognizing the liver as the primary site of drug metabolism, here we report a n...

To recreate or substitute tissue in vivo is a complicated endeavor that requires biomaterials that can mimic the natural tissue environment. Gelatin methacrylate (GelMA) is created through covalent bonding of naturally derived polymer gelatin and methacrylic groups. Due to its biocompatibility, GelMA receives a lot of attention in the tissue engine...

Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydr...

Anticancer nanomedicines have been studied over 30 years, but fewer than 10 formulations have been approved for clinical therapy today. Despite abundant options of anticancer drugs, it remains challenging to have agents specifically target cancer cells while reducing collateral toxicity to healthy tissue. Nanocompartments that can be selective towa...

Gaining precise control over the cellular entry pathway of nanomaterials is key in achieving cytosolic delivery, accessing subcellular environments, and regulating toxicity. However, this precise control requires a fundamental understanding of the behavior of nanomaterials at the bio-nano interface. Herein, we report a computational study investiga...

Inactive ingredients and generally recognized as safe compounds are regarded by the US Food and Drug Administration (FDA) as benign for human consumption within specified dose ranges, but a growing body of research has revealed that many inactive ingredients might have unknown biological effects at these concentrations and might alter treatment out...

Purpose The purpose of this study is to develop resin composite materials composed of polycaprolactone (PCL) acrylates and hydroxyapatite (HA) nanoparticles for ultraviolet digital light projection (DLP) three-dimensional (3D) printing technique. Design/methodology/approach Two PCL-based triacrylates, namely, glycerol-3 caprolactone-triacrylate (G...

Statement of problem: Three-dimensional printing has the potential for clinical applications, and additive manufacturing materials for dental use merit further investigation. Purpose: The purpose of this in vitro study was to evaluate the properties of materials formulated with ethoxylated bisphenol A-dimethacrylate (Bis-EMA), urethane dimethacr...

For stem cell differentiation, the microenvironment can play an important role, and hydrogels can provide a three dimensional (3D) microenvironment to allow native cell growth in vitro. A challenge is that the stem cell's differentiation can be influenced by the matrix stiffness. We demonstrate a low‐toxicity method to create different stiffness ma...

The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL)...

We demonstrated an approach that modifies the scaffold surface with a range of molecules, simultaneously conjugated to the scaffold by a single treatment with concentrated conditioned medium (CM), inducing mesenchymal stem cells (MSC) to differentiate into osteogenic lineages. We first show that the CM from MG63 cells is capable of inducing the des...

For many tissue engineering applications, an important goal is to create functional tissues in vitro. In order to make such tissues viable, they have to be vascularized. Bone marrow mesenchymal stem cells (BMSCs) are a promising candidate for vascularization. Growth supplements are commonly used to induce BMSCs differentiation, and further improvem...

Photoencapsulation of cells inside a hydrogel system can provide a suitable path to establish a gel in-situ, for soft tissue regeneration applications. However, the presence of photoinitiators and blue or UV light irradiation can result in cell damage and an increase of reactive oxygen species (ROS). We here evaluate the benefits of an antioxidant...

Lysophosphatidic acid (LPA) is a lipid mediator which binds to G‐protein‐coupled receptors and regulates various cellular functions. Interleukin‐1β (IL‐1β), a pro‐inflammatory cytokine, is elevated upon LPA treatment in human umbilical vein endothelial cells (HUVECs). Previous studies indicated that LPA upregulated vascular endothelial growth facto...