Chien-Yeong Wee

Chien-Yeong Wee
Institute for Agricultural Research and Development Malaysia · Biotechnology Research Centre

PhD

About

21
Publications
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Introduction
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Publications

Publications (21)
Article
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Fusarium oxysporum f. sp. cubense tropical race 4 ( Foc TR4) is a destructive necrotrophic fungal pathogen afflicting global banana production. Infection process involves the activation of programmed cell death (PCD). In this study, seven Musa acuminata vacuolar processing enzyme ( MaVPE1 – MaVPE7 ) genes associated with PCD were successfully ident...
Article
Full-text available
Pluronic F-68 (PF-68) is a non-ionic surfactant used in plant tissue culture as a growth additive. Despite its usage as a plant growth enhancer, the mechanism underlying the growth-promoting effects of PF-68 remains largely unknown. Hence, this study was undertaken to elucidate the growth-promoting mechanism of PF-68 using recalcitrant MR 219 callu...
Article
Full-text available
Antibiotic-adjuvant combinatory therapy serves as a viable treatment option in addressing antibiotic resistance in the clinical setting. This study was carried out to assess and characterize the adjuvant potential and mode of action of linalool against carbapenemase-producing Klebsiella pneumoniae (KPC-KP). Linalool exhibited bactericidal activity...
Article
Lignosulfonate (LS) is a commonly used to promote plant growth. However, the underlying growth promoting responses of LS in plant remain unknown. Therefore, this study was undertaken to elucidate the underlying growth promoting mechanisms of LS, specifically calcium lignosulfonate (CaLS). Addition of 100 mg/L CaLS in phytohormone-free media enhance...
Article
Full-text available
The Malaysian stingless bee industry is hugely dependent on wild colonies. Nevertheless, the availability of new queens to establish new colonies is insufficient to meet the growing demand for hives in the industry. Heterotrigona itama is primarily utilized for honey production in the region and the major source of stingless bee colonies comes from...
Article
Full-text available
This study was undertaken to evaluate growth-promoting effects of Pluronic F-68 (PF-68) on recalcitrant MR 219 rice callus. Our study shows that calli grown on Murashige and Skoog medium supplemented with 0.04% PF-68 significantly increased callus proliferation by 58.80% (fresh weight) and 23.98% (dry weight) while root formation from callus was en...
Article
Full-text available
Heterotrigona itama is a species of stingless bee recently domesticated (or reared) for honey production in a few Southeast Asian countries namely Malaysia and Indonesia. Being categorized in the clade Corbiculata together with the honeybees (Apis spp.) and bumble bees (Bombus spp.), the stingless bees are highly social in which the colony members...
Article
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The main objective of this study was to develop transgenic Malaysian Eksotika papaya with enhanced resistance to papaya dieback disease (PDD). Papaya dieback disease, which is caused by a Gram-negative bacterium, Erwinia mallotivora, has adversely affected Malaysia papaya industry and resulted in major production declines and economical losses. To...
Article
Full-text available
In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood in...
Article
Full-text available
Carica papaya L. or commonly known as papaya, is a major tropical crop consumed worldwide either as a vegetable or fresh fruit or processed products. In Malaysia, papaya was initially planted as a smallholder crop throughout the country. Eventually after 15 years of breeding and selection, a new variety, named C. papaya L. var. Eksotika, was releas...
Experiment Findings
Full-text available
Betik Eksotika (Carica papaya L. cv. Eksotika) merupakan salah satu varieti betik komersial yang penting di Malaysia. Namun, industri betik negara diancam oleh penyakit mati rosot betik yang memberi kesan negatif kepada penghasilan dan pengeluaran hasil betik tempatan. Bagi mengawal penyakit ini, satu strategi alternatif untuk mengganggu sistem kom...
Article
Dieback disease caused by Erwinia mallotivora is the most devastating disease of papaya in Malaysia. Most papaya cultivars here are susceptible to the disease, indicating a compatible relationship between the cultivars and the pathogenic strains of the bacteria. In this study, we evaluated early responses of a susceptible cultivar to a virulent str...
Article
Full-text available
Mitogen-activated protein kinase 4 (MPK4) interacts with the (Mitogen-activated protein kinase kinase kinase 1) MEKK1/ Mitogen-activated protein kinase kinase 1 (MKK1)/ Mitogen-activated protein kinase kinase 2 (MKK2) complex to affect its function in plant development or against pathogen attacks. The KEGG (Kyoto Encyclopedia of Genes and Genomes)...
Article
Full-text available
Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-...
Article
The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearin...
Article
Full-text available
Papaya is an important fruit crop in Malaysia for the domestic and export markets. The papaya industry is currently facing a major threat from the papaya dieback disease for which no effective control measure is known to prevent further spreading of the disease. The causal agent of the disease, Erwinia mallotivora was only identified lately. Hence,...
Article
Full-text available
Carotenoids, which naturally occurs as coloured pigment contributing to the attractive red colour in flowers and fruits, are C40 tetraterpenes biosynthesised from the central isoprenoid pathway. The Ggpps, Psy, Pds and Zds genes encode for the enzymes involved in catalysing sequent synthesis and desaturation reaction from isopentenyl pyrophosphate...

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