Chien-Kai Wang- PhD
- Professor (Associate) at National Chung Hsing University
Chien-Kai Wang
- PhD
- Professor (Associate) at National Chung Hsing University
Reproductive physiology, endocrinology and related behavior study in farm animals.
About
55
Publications
6,158
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Introduction
1. Research fields:
1.1 Animal Endocrinology
1.2 Reproductive Physiology
2. Current research
2.1 Immune regulation and health management during early pregnancy and transition period of dairy cows
2.2 Neuro-endocrine regulation in aggressive behaviours of chickens.
2. Research Experience:
2.1 Animal Endocrinology and Reproductive Physiology
2.2 Toxicity Assessment of Environment Air Pollutants
2.3 Chronic Pulmonary Disorders and Lung Cancer
2.4 Pre-clinical Evaluation of Natural Products for Cancer Therapy
Current institution
Additional affiliations
August 2015 - present
Position
- Professor (Assistant)
Description
- Animal physiology and experiment animal model.
July 2011 - June 2015
July 1998 - June 2000
Education
September 2001 - September 2005
September 1994 - June 1998
Publications
Publications (55)
This study describes a digital technique for uterine morphometry and its application to endometrial structure during the bovine oestrous cycle. Neither the number nor the size of uterine gland ducts changed during the cycle but a reduction in total endometrial area from days 0 to 8 after oestrus led to an increase in the proportion of the endometri...
Cooking oil fumes (COF) are known to be associated with respiratory diseases and risk of lung cancer. Involvement of trans , trans -2,4-decadienal ( tt -DDE), a major component in COF, is suspected.
Male CD-1® (ICR) mice were intratracheally instilled with either 8 or 24 mg·kg ⁻¹ tt -DDE weekly for 8 weeks. Total numbers and types of cells in bronc...
Negative energy balance (NEB) occurs in dairy cows during the transition period, during which time the blood concentrations of nonesterified fatty acids (NEFA) and metabolic ketones such as β-hydroxybutyric acid (BHBA) are elevated. These increased levels may disrupt immune functions and are regarded as risk factors associated with postparturient i...
The objective of the present study was to investigate the association among the largest follicle (LF), preovulatory estradiol (E2), and predominant vaginal epithelial cell at the completion of hormonal ovarian stimulation for fixed‐time artificial insemination (FTAI) in goats. Thirty‐seven crossbred Boer does received gonadotropin‐releasing hormone...
High levels of non-esterified fatty acids (NEFAs) during the transition period lead to increased oxidative stress and immunosuppression in cows. Feeding them a vitamin-E-supplemented diet reduces reactive oxygen species (ROS) levels in the blood and diminishes immunosuppression in the transition period. However, whether the restoration of immune ce...
In the poultry industry, broiler and layer strains are genetically selected for different purposes (e.g., high meat-yield and high egg-production). Genetic selection for productivity can have unintended consequences on the behavioral repertoire of the birds, including aggression. Alongside the increasing societal concern regarding the welfare of an...
Day-old male chick culling is one of the world’s most inhumane problems in the poultry industry. Every year, seven billion male chicks are slaughtered in laying-hen hatcheries due to their higher feed exchange rate, lower management than female chicks, and higher production costs. This study describes a novel non-invasive method for determining the...
Repeat breeding is a substantial problem in heifer and cow breeding leading to greater infertility for female dairy herds. The aim of present study was to investigate the impact of corpus luteum (CL) presence and category and the first gonadotropin-releasing hormone (GnRH) administration concurrent with exogenous progesterone (P4) treatment on the...
Background: Day-old male chicks culling is one of the world most inhumane problems in the poultry industry. Every year, seven billions of male chicks are being killed in laying-hen hatcheries, due to their higher feed exchange rate, lower management compare to female ones and production costs. This work reports a novel non-invasive method for the g...
Past immunological studies in broilers focused on juveniles within the rapid pre-slaughter growth period and may not reflect adult immune responses, particularly in breeders managed with chronic feed restriction (R). The study aimed to assess innate immune cell functions in respect to R vs. ad libitum (Ad) feed intake in breeder hens with and witho...
The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed’s adaptability to the area and disease
resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regen...
Many human diseases are inflammation-related, such as cancer and those associated with aging. Previous studies demonstrated that plasmon-induced activated (PIA) water with electron-doping character, created from hot electron transfer via decay of excited Au nanoparticles (NPs) under resonant illumination, owns reduced hydrogen-bonded networks and p...
Objective:
The purpose of this retrospective study was to investigate the relationship between temperature-humidity index (THI), season, and conception rate (CR) of Holstein cows in central Taiwan.
Methods:
The mean performance and number of observations were statistically evaluated for various parameters, including age at first service, number...
Pancreatic cancer remains the fourth leading cause of cancer-related death in the USA with a 5-year survival rate of 5 %. The effects of epidermal growth factor receptor and vascular endothelial growth factor A blockade with chemotherapy on pancreatic tumor growth were examined. Mice bearing human PANC-1 cell xenografts were divided into three grou...
The objective of this study is to evaluate the efficacy and safety profiles of the targeted medications, bevacizumab and cetuximab, in combination with cytostatic drugs in patients with locally advanced or metastatic pancreatic cancer.
In this retrospective phase 2 study, a total of 59 patients with pancreatic cancer were recruited and received con...
Current chemotherapeutic regimens for nonsmall cell lung cancer (NSCLC) have reached a plateau over the last few years. Targeted therapy makes use of tyrosine kinase inhibitors (TKIs) to suppress a number of signaling pathways including epidermal growth factor receptor and vascular endothelial growth factor which are active in NSCLC biology. In thi...
Chemotherapy is a major clinical treatment for managing patients with advanced and recurrent ovarian cancer. However, the clinical performance of chemotherapy is limited, and adverse effects have been observed. Integrating chemotherapy with current chemotherapeutic drugs and novel antitumor ingredients might improve the clinical performance of curr...
Taiwanofungus camphoratus
(synonym
Antrodia camphorata
) is a widely used medicinal fungus in the folk medicine of Taiwan with several pharmacological features such as anti-inflammatory, liver protection, antihypertensive, and antioxidative activities. The ethanolic extract of
T. camphoratus
(TCEE) which contains abundant bioactive compounds includ...
Chemotherapy is the main approach for treating advanced and recurrent hepatocellular carcinoma (HCC), but the clinical performance of chemotherapy is limited by a relatively low response rate, drug resistance, and adverse effects that severely affect the quality of life of patients. The aqueous extract of
Solanum nigrum
(AE-SN) is a crucial ingredi...
Cell-based systems are useful for discovering antiviral agents. Dissecting the viral life cycle, particularly the early entry stages, allows a mechanistic approach to identify and evaluate antiviral agents that target specific steps of the viral entry. In this report, the methods of examining viral inactivation, viral attachment, and viral entry/fu...
Fermented wheat germ extract (FWGE) is a nutrient supplement and a potential antitumor ingredient for developing an integrated chemotherapy with standard chemotherapeutic drugs for treating ovarian cancer patients. In this study, we evaluated the tumor suppression efficiency of FWGE in human ovarian carcinoma cells, SKOV-3 and ES-2, and found the h...
The aqueous extracts of the leaves and fruit of Camptotheca acuminata have long been used in traditional Chinese medicine (TCM) for treating cancer patients. The chemotherapeutic drug, camptothecin (CPT), and related analogs were first isolated from C. acuminata in the 1970s. Although the antitumor effects of CPT have been characterized in recent y...
Cervical cancer is an important health issue for women worldwide, and the endoplasmic reticulum stress pathway is important for determining the chemotherapeutic response to cancer. However, the role of glucose-regulated protein 94 (GRP94) in taxane therapy for cervical cancer remains unclear. In this study, we generated GRP94 knockdown (GRP94-KD) H...
Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were...
Thrombomodulin (TM) has been shown to regulate many physiological and pathological processes, including inflammation, thrombosis, and tumor progression. TM is also a natural anticoagulant that maintains circulatory homeostasis in endothelial cells. However, little is known regarding the role of TM in the progression and metastasis of cervical cance...
Colorectal cancer is a common cancer worldwide, and chemotherapy is a mainstream approach for advanced and recurrent cases. Development of effective complementary drugs could help improve tumor suppression efficiency and control adverse effects from chemotherapy. The aqueous extract of Solanum nigrum leaves (AE-SN) is an essential component in many...
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related death worldwide. Due to the difficulties of early diagnosis, curative treatments are not available for most patients. Palliative treatments such as chemotherapy are often associated with low response rate, strong adverse effects and limited clinical benefits for patie...
Chemotherapy is the main approach in dealing with advanced and recurrent endometrial cancer. An effective complementary ingredient can be helpful in improving the clinical outcome. Aqueous extract of Solanum nigrum leaf (AE-SN) is a principal ingredient for treating cancer patients in traditional Chinese medicinal practice but lacks sufficient evid...
Background:
Human CYP2A6 and CYP2A13 play important roles in metabolic activation of many pulmonary carcinogens and thus their expression and distribution may determine the pulmonary susceptibility to metabolically activated carcinogens and the following lung cancer development. Because of the 93.5% of amino acid identity between CYP2A6 and CYP2A1...
The locations of sampling grouper fish farms in Taiwan. Taiwan is located between tropical and subtropical regions. The grouper aquacultures are mainly gathered in southern Taiwan due to the grouper fish preferred warm water temperature. Black circles indicate five major regions of sampling grouper aquaculture farms. The open circle indicates the l...
The electropherograms of the RT-PCR products from purified RNA. The minimum concentration detected on the CE module was 3 copies/µL (Kuo et al. unpublished). Each sample includes a mixture of DNA markers and RT-PCR products obtained from the infected grouper. Eleven peaks (corresponding to the DNA markers, added to samples post-amplification) and a...
Evaluation of multiplex RT-PCR with ex vivo samples (infected fish). M. DNA marker; lane 1, NNV (nodavirus); lane 2, V. anguillarum; lane 3, iridovirus; lane 4, NNV (nodavirus)+V. anguillarum; lane 5, NNV (nodavirus)+iridovirus; lane 6, V. anguillarum+iridovirus; lane 7, NNV (nodavirus)+V. anguillarum+iridovirus.
(TIF)
Chronic obstructive pulmonary diseases (COPD) have been the major cause of mortality worldwide. Early identification of populations at risk allows us to prevent the occurrence and to reduce the cost of health care. In human studies, exposure to environmental tobacco smoke (ETS) and arsenic respectively increased the risk of chronic lung diseases, i...
Infection of virus (such as nodavirus and iridovirus) and bacteria (such as Vibrio anguillarum) in farmed grouper has been widely reported and caused large economic losses to Taiwanese fish aquaculture industry since 1979. The multiplex assay was used to detect dual viral infection and showed that only nervous necrosis virus (NNV) can be detected t...
Although 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is classified as a human carcinogen, TCDD only induced oxidative DNA damages. In our present study, we combined TCDD with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) to investigate their tumorigenic effects on lung tumor formation in A/J mice. Application of NNK at a tumorigenic dose (2 m...
We had previously reported that aryl hydrocarbon receptors (AhRs) are overexpressed in lung adenocarcinomas. Benzo[a]pyrene (BaP), an AhR agonist, increased FGF-9 expression in human lung adenocarcinoma cells. Similarly, several AhR agonists increased FGF-9 mRNA levels, and BaP-induced FGF-9 expression was prevented by cotreatment with AhR antagoni...
The association of aryl hydrocarbon receptor (AhR) overexpression with cytochrome P450 1B1 (CYP1B1) expression is commonly detected in non-small cell lung cancer (NSCLC). In vitro and animal studies have shown an interaction between AhR and p53, or the epidermal growth factor receptor (EGFR). The clinical importance of AhR or CYP1B1 overexpression,...
Background: The association of aryl hydrocarbon receptor (AhR) overexpression with cytochrome P450 1B1 (CYP1B1) expression is commonly detected in non-small cell lung cancer (NSCLC). In vitro and animal studies have shown an interaction between AhR and p53, or the epidermal growth factor receptor (EGFR). The clinical importance of AhR or CYP1B1 ove...
We aimed to produce an estrogen-responsive reporter plasmid that would permit monitoring of estrogen receptor function in the uterus in vivo. The plasmid pBL-tk-CAT(+)ERE was induced by estrogen in bovine endometrial stromal cells. When the CAT gene was replaced by the secreted alkaline phosphatase SeAP, the resulting construct pBL-tk-SeAP(+)ERE re...
The angiopoietin/Tie receptor system may contribute to angiogenesis and vascular remodeling by mediating interactions of endothelial cells with smooth muscle cells and pericytes. The temporal expression of angiopoietin-1 (Angpo-1), angiopoietin-2 (Angpo-2), Tie-1, and Tie-2 mRNA was studied in a focal cerebral ischemia model in rats. The cDNA fragm...
Iron may catalyse the production of reactive oxygen species during post-ischemic reoxygenation and subsequently lead to brain damage. Ferritin, an iron sequestering and storage protein, can also be a source of iron after ischemic insult. However, its role in ischemia-reperfusion has not been carefully investigated. In the present study, we examined...
In the present study, we examined the temporal and spatial expression profiles of GFAP mRNA and protein in a focal cerebral ischemia model with ischemic injury confined to the cerebral cortex in the right middle cerebral artery (MCA) territory. Northern blot analysis showed a respective 5.5-fold and 7.2-fold increase in the GFAP mRNA in the ischemi...
