Chevance Soizic

Chevance Soizic
Université de Rennes 1 | UR1 · Institut des Sciences Chimiques de Rennes (ISCR) - UMR CNRS 6226

Doctor of Structural Biology
Assitant Professor in Chemisrty - University of Rennes - Institute of Chemical Sciences of Rennes (UMR 6226 CNR

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69
Publications
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Publications

Publications (69)
Article
Glioblastoma remains a cancer for which the effectiveness of treatments has shown little improvement over the last decades. For this pathology, multiple therapies combining resection, chemotherapy and radiotherapy remain the norm. In this context, the use of high-Z nanoparticles such as gold or hafnium to potentiate radiotherapy is attracting more...
Article
Exposure of consumers to aluminum-containing nanomaterials (Al NMs) is an area of concern for public health agencies. As the available data on the genotoxicity of Al2O3 and Al⁰ NMs are inconclusive or rare, the present study investigated their in vitro genotoxic potential in intestinal and liver cell models, and compared with the ionic form AlCl3....
Article
Particles stabilize fluid interfaces. In particular, oil/water Pickering emulsions undergo limited coalescence, yielding droplets of smaller size as the amount of particles is increased. Herein, we studied the effect of hydrophobic nanoparticles (< 10 nm, alkyl-coated) on submicronic droplets (ca 100 nm) formed in an Ouzo system. We investigated th...
Preprint
Full-text available
Background: Exposure of consumers to aluminum-containing nanomaterials (Al NMs) through numerous products is an area of concern for public health agencies since human health risks are not completely elucidated. In addition, the available data on the genotoxicity of Al2O3 and Al⁰ NMs are inconclusive or rare. In order to provide further information,...
Article
A growing body of evidences indicate the major role of extracellular vesicles (EVs) as players of cell communication in the pathogenesis of liver diseases. EVs are membrane-enclosed vesicles released by cells into the extracellular environment. Oxidative stress is also a key component of liver disease pathogenesis, but no role for hepatocyte-derive...
Article
We investigate the encapsulation in Hybridosomes®, a type of capsules unique regarding their structure and method of elaboration. Hybridosomes®, are made of a single shell of nanoparticles (~5 nm) crosslinked with a polymer and are easily obtained via spontaneous emulsification in a ternary mixture THF/water/butylated hydroxytoluene (BHT). Our main...
Article
Full-text available
Extracellular vesicles (EVs) are membrane enclosed nanostructures released by cells into the extracellular environment. As major actors of physiological intercellular communication, they have been shown to be pathogenic mediators of several liver diseases. EVs also appear to be potential actors of drug-induced liver injury, but nothing is known con...
Article
Aluminum (Al) can be ingested from food and released from packaging and can reach key organs involved in human metabolism, including the liver via systemic distribution. Recent studies discuss the occurrence of chemically distinct Al-species and their interconversion by contact with biological fluids. These Al species can vary with regard to their...
Article
Full-text available
Background and Aims: Extracellular vesicles (EVs), membrane enclosed nanostructures released by cells into the extracellular environment, have been described as mediators in several pathogenic processes including liver diseases. In regards to xenobiotic liver injury, EVs emerge as potential actors of DILI (Drug Induced Liver Injury), but nothing i...
Article
Full-text available
Latent membrane protein 1 (LMP1) encoded by the Epstein-Barr virus (EBV) plays an important role in EBV-induced cell transformation. Down-regulation of the LMP1 expression had shown promising results on cancer cell therapy. In this study, we identified by Phage display a novel peptide called B1.12 (ACPLDLRSPCG) which selectively binds to the extrac...
Article
Useful properties render titanium dioxide nanomaterials (NMs) to be one of the most commonly used NMs worldwide. TiO2 powder is used as food additives (E171), which may contain up to 36% nanoparticles. Consequently, humans could be exposed to comparatively high amounts of NMs that may induce adverse effects of chronic exposure conditions. Visualiza...
Article
Full-text available
Herein we present examples of the application of the 13C-13C Nuclear Overhauser Effect Spectroscopy (NOESY) experiment to the study of metalloproteins and we critically discuss the advantages and drawbacks of the method as a function of the molecular size of the investigated systems. The contribution is focused on a few case studies among the syste...
Article
Full-text available
Metal clusters Nanoparticles are obtained by a simple solvent shifting procedure called the Ouzo effect. Remarkably, the assembly of [{Mo6Br8}L6]2- (L=Br- or NCS-) cluster units can be directed into nanomarbles...
Article
Aluminum (Al) is one of the most common elements in the earth crust and increasingly used in food, consumer products and packaging. Its hazard potential for humans is still not completely understood. Besides the metallic form, Al also exists as mineral, including the insoluble oxide, and in soluble ionic forms. Representatives of these three specie...
Article
Full-text available
The application of appropriate analytical techniques is essential for nanomaterial (NM) characterization. In this study, we compared different analytical techniques for NM analysis. Regarding possible adverse health effects, ionic and particulate NM effects have to be taken into account. As NMs behave quite differently in physiological media, speci...
Article
Full-text available
Hollow nanocapsules (named Hybridosomes®) possessing a polymer/nanoparticles shell were used to covalently construct hybrid films in a one-pot fashion. Alkyne bearing organic/inorganic Hybridosomes® were reticulated with azide bearing homobifunctional polyethyleneglycol (PEG) linkers, by using an electro-click reaction on F-SnO2 (FTO) electrodes. T...
Article
A number of nanoparticles has been developed by chemists for biomedical applications to meet imaging and targeting needs. In parallel, adoptive T therapy with chimeric antigen receptor engineered T cells (CAR T cells) has recently held great promise in B-cell malignancy treatments thanks to the development of anti-CD19 CAR T cells. Indeed, CD19 is...
Patent
The present invention relates to the field of nanomaterials. More particularly, the present invention relates to new nano-objects, Hybridosomes®, consisting of an aqueous core and an organic-inorganic hybrid shell. The shell comprises both inorganic nanoparticles and polymer chains. The present invention also relates to a method for preparing the h...
Article
Full-text available
Our group recently introduced a new process to synthesize nanoparticle shells of about 100nm, named “hybridosomes®”. Here, the structure and the mechanical properties of hybridosomes® made from iron oxide nanoparticles and poly(acrylic acid) are characterized using TEM, AFM and an osmotic compression technique. For this latter, the size distributio...
Cover Page
In the Communication featured on the Inside Cover, the authors use the unsuspected presence of nanodroplets (∼100 nm) in water/THF mixtures to template the formation of hybrid nanocapsules, “hybridosomes”, with a shell consisting of a crosslinked network of particles and polymers. Magnetic hybridosomes prepared from iron oxide nanoparticles enhance...
Article
A general method to generate hybrid hollow capsules is reported. The process is based on the stabilization of solvent droplets by nanoparticles, in macroscopically miscible mixtures of tetrahydrofuran (THF) and water. After addition of a crosslinking polymer and removal of the solvent core, capsules of diameter ca 100 nm are obtained. This novel st...
Article
Monometallic gold(I)-alkynyl-helicene complexes (1 a,b) and bimetallic gold(I)-alkynyl-helicene architectures featuring the presence (2 a,b) or absence (3 a,b) of aurophilic intramolecular interactions were prepared by using different types of phosphole ligands (mono-phosphole L1 or bis-phospholes L2,3). The influence of the Au(I) d(10) metal cente...
Article
The metal complex FeTSPPCl (5,10,15,20-tetrakis)-(4-sulfonato-phenyl)-porphyrin-iron(III) chloride is an active catalyst for carbenoid insertion in NH bonds of aminoacid derivatives in aqueous media. A variety of diazoacetates and methyl diazophosphonate were used as carbenoid precursors. The commercially available iron porphyrin complex can also s...
Article
The development of environmentally benign reactions is an important goal in synthetic organic chemistry and chemical engineering. However, catalytic enantioselective reactions using transition-metal complexes in protic or aqueous solvents are within limited ranges. The current applications of asymmetric catalysis using metalloporphyrins will be rep...
Article
Full-text available
The use of peptidic ligands is validated as a generic chemical platform allowing one to finely control the organization in solid phase of semiconductor nanorods originally dispersed in an aqueous media. An original method to generate, on a macroscopic scale and with the desired geometry, three-dimensional supracrystals composed of quantum rods is i...
Article
Full-text available
Symmetry plays a fundamental role in chiral recognition and enantioselective catalysis. Porphyrins possess a number of structural features that make them attractive for the stereocontrol of chiral recognition and metal-catalyzed asymmetric reactions. This article is a brief account of our studies on chiral recognition and enantioselective catalysis...
Article
The development of environmentally benign reactions is an important goal in synthetic organic chemistry and chemical engineering. However, catalytic enantioselective oxidations using transition-metal complexes are limited when the oxidant is hydrogen peroxide. The two main difficulties of using hydrogen peroxide in the presence of transition metal...
Article
The direct reduction of carboxylic acids to disilylacetals was achieved through a manganese catalyzed hydrosilylation reaction in the presence of triethylsilane under mild conditions, at r.t. and under UV irradiation (350 nm). The aldehydes were obtained in good to excellent yields after acidic hydrolysis.
Article
Full-text available
An efficient asymmetric oxidation of sulfides catalyzed by water-soluble chiral manganese porphyrin was carried out in presence of cheap and environmentally benign oxidant H2O2 at 25 °C. Prochiral sulfides were converted to respective sulfoxides with up to 100% conversion and up to 57% enantiomeric excess. The present study demonstrated the necessi...
Article
Full-text available
In addition to its electron transfer activity, cytochrome c is now known to trigger apoptosis via peroxidase activity. This new function is related to a structural modification of the cytochrome upon association with anionic lipids, particularly cardiolipin present in the mitochondrial membrane. However, the exact nature of the non-native state ind...
Article
Full-text available
In addition to its electron transfer activity, cyto-chrome c is now known to trigger apoptosis via peroxidase activity. This new function is related to a structural modification of the cytochrome upon association with anionic lipids, particularly cardiolipin present in the mitochondrial membrane. However, the exact nature of the non-native state in...
Article
The asymmetric 3-ethyl-2-methylporphyrin iron complex was synthetized and inserted into apomyoglobin. UV-visible spectroscopic studies demonstrated the capacity of iron to coordinate different exogenous axial ligands in ferrous and ferric forms. The position of synthetic heme into the hydrophobic pocket of the reconstituted myoglobin was investigat...
Data
Plot of the chemical shift variation (Garrett values) of the backbone Bcl-xL amide signals in the cytochrome c−Bcl-xL adduct. The horizontal line indicates the selected chemical shift perturbation threshold. (TIF)
Data
Plot of the chemical shift variation (Garrett values) of the backbone cytochrome c amide signals in the cytochrome c−Bcl-xL adduct. The horizontal line indicates the selected chemical shift perturbation threshold. (TIF)
Data
Acquisition parameters for the NMR experiments; all spectra were acquired at 300 K. (PDF)
Data
Fitting of the weighted average chemical shift variations of three perturbed residues (Leu90, Gly94, Gly200) of the 15N-enriched Bcl-xL as a function of the concentration of the unlabeled cytochrome c. (TIF)
Data
Full-text available
HADDOCK active residues for Bcl-xL and cytochrome c. (PDF)
Data
Structural statistics calculated over all structures for the 4 clusters obtained by HADDOCK calculations. (PDF)
Article
Full-text available
A structural model of the adduct between human cytochrome c and the human anti-apoptotic protein Bcl-xL, which defines the protein-protein interaction surface, was obtained from solution NMR chemical shift perturbation data. The atomic level information reveals key intermolecular contacts identifying new potentially druggable areas on cytochrome c...
Article
Direct and remarkably fast electron transfers between a bare glassy carbon electrode and heme proteins (hemoglobin or myoglobin) are obtained by using an aqueous 1-butyl-3-methyl imidazolium tetrafluoroborate (BMI.BF(4)) ionic-liquid mixture as electrolyte. The ionic liquid is observed to play a key role in the achievement of the electron transfer....
Article
The complexation of optically active or racemic amines, amino esters and amino acids with chiral ruthenium Halterman porphyrins is described. For various amino esters, chiral recognition was observed for the complexation of the ligand with up to 60% enantiomeric excess for 1-(1-naphthyl) ethylamine. A water-soluble ruthenium Halterman porphyrin, du...
Article
The transfer of the metallocarbene derived from N- and O-protected 6-diazo-5-oxo-l-norleucine (DON) catalyzed by metalloporphyrins undergoes dimerization, cyclopropanation, N–H and S–H insertion reactions, respectively. An efficient and direct synthesis of 5-oxo-l-pipecolic acid from DON is described from unprotected 6-diazo-5-oxo-l-norleucine.
Article
Inhibition of histone deacetylases (HDACs) leads to growth arrest, differentiation, or apoptosis of tumor cell lines, suggesting HDACs as promising targets for cancer therapy. At present, only one HDAC inhibitor (HDACi) is used in therapy: suberoylanilide hydroxamic acid (SAHA). Here, we describe the synthesis and biological evaluation of a new ser...
Article
Full-text available
Nuclear receptors are ligand-activated transcription factors involved in all major physiological functions of complex organisms. In this respect, they are often described as drugable targets for a number of pathological states including hypercholesterolemia and atherosclerosis. HNF4alpha (NR2A1) is a recently 'deorphanized' nuclear receptor which i...
Article
Full-text available
Detailed characterization of protein, peptide or drug interactions with natural membrane is still a challenge. This review focuses on the use of nuclear magnetic resonance (NMR) for the analysis of interaction of molecules with small unilamellar vesicles (SUV). These phospholipid vesicles are often used as model membranes for fluorescence or circul...
Article
The molecular tumbling of small unilamellar vesicles is not fast enough to enable the detection of (1)H NMR signals of molecules associated with phospholipids. We show that relatively fast kinetic exchange of the interacting molecules is able to induce a strong decrease of the residual homonuclear dipolar coupling, allowing the acquisition of sharp...
Article
[structure: see text] A solid-phase fluorenylmethyloxycarbonyl (Fmoc)-based synthesis strategy is described for "mixed" aza-beta3-peptides as well as a convenient general approach for their required building blocks, the aza-beta3-amino acid residues (aza-beta3-aa). These monomers allow the synthesis of relatively large quantities of pure mixed aza-...
Article
Fourier transform infrared (FTIR) difference spectroscopy allows the study of molecular changes occurring at active sites in proteins with high sensitivity. Reactions are triggered by light, potential, or temperature steps and more recently by the diffusion of buffers containing effectors above membrane proteins deposited as films on ATR crystals....
Article
Saccharomices cerevisiae (yeast iso-1) cytochrome c has been investigated in the presence of 100 mM SDS in order to simulate the interaction of cytochrome c with membrane. Under these circumstances, a high spin species with detached methionine axial ligand is observed through NMR, in analogy to findings on the horse heart protein. However, at varia...
Article
The interaction of cytochrome c with micelles of sodium dodecyl sulfate was studied by proton NMR spectroscopy. The protein/micelles ratio was found to be crucial in controlling the extent of the conformational changes in the heme crevice. Over a range of ratios between 1:30 and 1:60, the NMR spectra of the ferric form display no paramagnetic signa...

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Projects (3)
Project
What mode of action do nanomaterials ingested via food have in liver and intestine? Which factors determine their toxicity? Due to the large number of different nanomaterials, it is hardly possible to test every one for its toxic properties. For this reason, specific properties for the classification of nanomaterials are to be examined within the scope of the Franco-German research project "SolNanoTox".
Project