Chengjun Huang

Chengjun Huang
Institute of Microelectronics of Chinese Academy of Sciences (IMECAS) · e-Health Department

Ph.D

About

84
Publications
8,459
Reads
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1,048
Citations
Introduction
- Micro/nano sensors, actuators and systems - MEMS device design, simulation and processing - Microfluidics and lab-on-a-chip - Biomedical microdevices and systems, electronics for healthcare
Additional affiliations
May 2016 - present
The University of Chinese Academy of Sciences
Position
  • Professor (Full)
Description
  • A adjunct professor of UCAS
January 2014 - March 2020
Institute of Microelectronics of Chinese Academy of Sciences (IMECAS)
Position
  • Head of Department
January 2007 - December 2013
imec
Position
  • Researcher

Publications

Publications (84)
Article
Cellular adherent morphology and suspended electrical property are two important intrinsic biophysical features of single cells in two states. However, few studies reported their relationship due to lacking systematic methods. Here, we proposed a toolchain for enriching, proliferating single cells from pleural effusions (PEs), and characterizing th...
Article
Due to its high sensitivity and temporal resolution, surface plasmon resonance microscopy (SPRM) is currently widely used in biological analysis. In this review, we briefly introduce the principle and setup of SPRM. Applications in label-free biological analysis are also indicated, including fast detection to single cells, exosomes, viruses, and mo...
Preprint
Full-text available
Exosomes are highly important in clinical diagnosis due to their high homology with their parental cells. However, conventional exosome detection methods still face the challenges of expensive equipment, low sensitivity, and complex procedures. Field effect transistors (FETs) are not only the most essential electronic component in the modern microe...
Article
Owing to the label-free and real-time biosensing advantages, the nanostructured plasmonic biosensors on optical fiber tips have attracted increasing attention as potential plug-and-play remote sensing probes available for limited environments. Various nanostructures have been developed by either top-down or bottom-up approaches on optical fiber tip...
Article
Full-text available
Plasmonic imaging has exhibited superiority in label-free and fast detection to single nanoparticles due to its high sensitivity and high temporal resolution, which plays an important role in environmental monitoring and biomedical research. As containing plenty of information associated with particle features, plasmonic imaging has been used for i...
Article
The sieving and enrichment of rare tumor cells from large-volume pleural effusion (PE) samples is a promising technique for cell-based lung tumor diagnosis and drug tests, which features high throughput and recovery, purification, as well as viability rates of rare target cells as the prerequisites for high sensitivity, specificity, and accuracy of...
Article
In this letter, we report a performance enhancement approach for MEMS thermopile Pirani sensors through in-situ integration of nanoforests. The nanoforests not only increase the light absorption of the thermopile, resulting in an improved output voltage, but also increase the contact area and heat exchange sites for gas molecules due to their high...
Article
Full-text available
Real-time, high-sensitivity, and label-free detection to single nanoparticles has been achieved via visualizing the interaction between surface plasmon polaritons (SPPs) and nanoparticles, which is widely applied to chemistry and biology. In this work, aiming to enhance the detection sensitivity to nanoparticles, we explore the interaction of SPP s...
Article
Three-dimensional (3D) surface-enhanced Raman scattering (SERS) devices have been extensively studied owing to their unique structural advantages. In particular, 3D metal/semiconductor heterostructures with synergistic contribution of plasmonic “hot spots” and charge transfer resonance can exhibit a significant SERS effect. In this work, a novel ty...
Article
Tumor-derived exosomes have been recognized as potential biomarkers for cancer diagnosis because they are actively involved in cancer progression and metastasis. However, progress in practical exosome analysis is still slow due to the limitation in exosome isolation and detection. The development of microfluidic devices has provided a promising ana...
Article
An angle-interrogated surface plasmon resonance (SPR) sensor based on a prism-coupled configuration has been extensively applied in biomedicine, environment monitoring, and food safety. Yet, the low sensitivity and low spatial resolution impede its further development. In this Letter, we investigated objective-coupled locally excited SPR for refrac...
Article
Full-text available
The development of surface-enhanced Raman scattering (SERS) devices for detection of trace pesticides has attracted more and more attention. In this work, a large-area self-assembly approach assisted with reactive ion etching (RIE) is proposed for preparing SERS devices consisting of Ag-covered “hedgehog-like” nanosphere arrays (Ag/HLNAs). Such a S...
Article
Full-text available
Three-dimensional nanowire arrays based on different biomaterials have emerged as promising platforms for the studies of cell behaviours over the years. Fabrication of nanosurfaces with complex geometries and controllable morphologies is still a challenge. In this work, nanowire forests (NWFs), which exhibited various aspect ratios, were fabricated...
Article
Full-text available
Plasmonic interferometric imaging has been widely used for fast and label-free detection to nano-objects, which associates the interference between launched and scattered surface plasmon polaritons (SPPs). As scattering of SPPs is affected by the characteristics of nano-objects, particle size, particle index and particle-interface distances have be...
Preprint
Full-text available
Real-time, high-sensitivity, and label-free detection to single nanoparticles has been achieved via visualizing the interaction between surface plasmon polaritons (SPPs) and nanoparticles, which is widely applied to chemistry and biology. In this work, aiming to enhance the detection sensitivity to nanoparticles, we explore the interaction of SPP s...
Chapter
Exosomes are extracellular nano-scale vesicles with a diameter of about 40–100 nm and a density of about 1.13–1.19 g/ml, which are secreted by most cells and have phospholipid bilayer structure on their surface. Exosomes contain a large amount of genetic information and a variety of proteins involved in cell–cell communication and macromolecular tr...
Article
Full-text available
By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scatteri...
Article
Self-assembly at the air/water interface (AWI) has proven to be an efficient strategy for fabricating two-dimensional (2D) colloidal monolayers, which was widely used as the template for nanosphere lithography in nanophononics, optofluidics, and solar cell studies. However, the monolayers fabricated at the AWI usually suffer from a small domain are...
Article
Full-text available
The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporatio...
Article
Full-text available
Circulating tumor cells (CTCs), a type of cancer cell that spreads from primary tumors into human peripheral blood and are considered as a new biomarker of cancer liquid biopsy. It provides the direction for understanding the biology of cancer metastasis and progression. Isolation and analysis of CTCs offer the possibility for early cancer detectio...
Article
Full-text available
Neutrophil chemotaxis plays a vital role in human immune system. Compared with traditional cell migration assays, the emergence of microfluidics provides a new research platform of cell chemotaxis study due to the advantages of visualization, precise control of chemical gradient, and small consumption of reagents. A series of microfluidic devices h...
Article
Full-text available
Label-free, fast, and single nanoparticle detection is demanded for the in situ monitoring of nano-pollutants in the environment, which have potential toxic effects on human health. We present the label-free imaging of single nanoparticles by using total internal reflection (TIR)-based leakage radiation microscopy. We illustrate the imaging of both...
Article
In this work, we develop a new method for fabricating wafer-level gold nanoparticles covered silicon nanopillars (SNPs) combined with surface chemical modification to detect trace level carbonyl compounds based on surface-enhanced Raman scattering (SERS) technique. The SNPs are fabricated with an etching process using nano masks synthesized in oxyg...
Article
Full-text available
Due to their excellent physical and chemical properties, graphene sheets are widely used in industry, which makes detection important to guarantee their performance. Atomic force microscopy, scanning electron microscopy, and Raman spectroscopy are the most common detection methods, which is either time-consuming or easily destructive. In this work,...
Article
In this erratum, the function ${\lambda _{{\rm SPP}}}$λSPP in the third page of Opt. Lett.44, 5707 (2019)OPLEDP0146-959210.1364/OL.44.005707 has been corrected.
Conference Paper
The directional scattering in low-index dielectric nanoparticles is hard to detect, as the multipole resonances are weak. With surface plasmon polariton (SPP) induced multipole moments, we observed the directional SPP scattering by single polystyrene nanoparticles.
Conference Paper
We presented a method to manipulate the surface plasmon propagation by using the total reflection of single hollow nanoparticle. We used plasmonic imaging to observe the manipulation by single hollow nanoparticle in different surrounding medium.
Article
Label-free single-nanoparticle detection is crucial for the fast detection of nanoparticles and viruses in environmental monitoring and biological sciences. In this Letter, benefiting from the leakage radiation that transforms the near-field surface plasmon polariton (SPP) distribution along the interface to the far field, we demonstrated the plasm...
Article
This paper presents a crossing constriction channel based microfluidic system for high-throughput characterization of specific membrane capacitance (Csm) and cytoplasm conductivity (σcy) of single cells. In operations, cells in suspension were squeezed through the major constriction channel and effectively seal rather than invading into the side co...
Article
Full-text available
Carbonyl compounds in water sources are typical characteristic pollutants, which are important indicators in the health risk assessment of water quality. Commonly used analytical chemistry methods face issues such as complex operations, low sensitivity, and long analysis times. Here, we report a silicon microfluidic device based on click chemical s...
Article
Full-text available
The quality of edge detection is related to detection angle, scale, and threshold. There have been many algorithms to promote edge detection quality by some rules about detection angles. However these algorithm did not form rules to detect edges at an arbitrary angle, therefore they just used different number of angles and did not indicate optimize...
Article
Capillary-driven microfluidic devices have a great potential for the point-of-care testing systems based on the advantages of self-pumping, low reagent usage and rapid detection. Here, the study presents a lidless Si-based capillary-driven microfluidic device, comprising two inlets for sample and buffer loading, a snake-shaped microchannel (120/0.0...
Article
Full-text available
Surface-enhanced Raman scattering (SERS) nanoprobes show promising potential for biosensing and bioimaging applications due to advantageous features of ultrahigh sensitivity and specificity. However, very limited research has been reported on the SERS photostability of nanoprobes upon continuous laser irradiation, which is critical for high-speed a...
Article
This paper presents a new microfluidic impedance cytometry with crossing constriction microchannels, enabling the characterization of cellular electrical markers (e.g., specific membrane capacitance (Csm) and cytoplasm conductivity (σcy)) in large cell populations (~ 100,000 cells) at a rate greater than 100 cells/s. Single cells were aspirated con...
Article
Full-text available
This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Pyt...
Patent
Abstract: A microfluidic magnetic selector comprises a microfluidic channel comprising at least one bifurcation, forming a selection portion of the selector and splitting the microfluidic channel into a main channel and at least one selection channel; at least one magnetic flux concentrator for concentrating a magnetic flux at the level of the b...
Patent
The present disclosure provides a cell positioning unit, array, device and a method for manufacturing the same. The cell positioning unit comprises: a substrate; at least a pair of microelectrodes on the substrate, wherein the microelectrodes are disposed at intervals on a circumference such that pDEP force are generated in an area where tips of th...
Conference Paper
Dean flow in a spiral microchannel is simulated systematically using the set of Navier Stokes equations. The influence of fluid flow rate and spiral initial radius on Dean flow is discussed. The particals' motion under the Dean drag force in the spiral microchannel is shown in the last.
Conference Paper
In this paper, we present an original concept of plasmonic-related instrumentation platform dedicated to diagnostic biosensing tests out of the laboratory. The developed instrumental platform includes both disposable one-use microfluidic affinity biochip and compact optical readout device for biochip monitoring involving mobile Internet devices for...
Article
Non-small-cell lung cancer (NSCLC) comprises �75% of all lung cancer and consists of several subtypes. Identification of lung cancer cell subtypes is important for choosing the appropriate therapy plan and reducing mortality. In this study, we have been able to identify and distinguish three subtypes of NSCLC cells (H1229, H460 and A549) and leukoc...
Conference Paper
The spectral imaging technology is able to analysis the spectra l and spatial geometric character of the target at the same time. To break through the limitation brought by the size, weight and cost of the traditional spectral imaging instrument, a miniaturized novel spectral imaging based on CMOS processing has been introduced in the market. This...
Conference Paper
To break the limitation of the traditional spectral analysis system in low speed, high cost and huge size, this paper presents a CMOS-based monolithically miniaturized spectral system whose core component is the spectral imager. The idea of the spectral imager is to fabricate a spectral filter on top of the traditional CMOS imager. This paper desig...
Article
The solid-state nanopore-based detection technique is attracting extensive research interests, which can be widely used for various applications, such as nanoparticle detection, biomolecule analysis and DNA sequencing. In this study, two types of nanopores, the cylindrical nanopore and the pyramidal-shaped nanopore, were systematically studied in s...
Article
Full-text available
Single-nanoparticle detection with cylindrical and pyramidal-shaped nanopores was systematically studied using FEM (Finite Element Modeling) method simulations and terminal currents were obtained. The effects of pore size, length and the ratio of particle and pore diameter on the blocked current were discussed. Based on the obtained results, an opt...
Conference Paper
Full-text available
This paper presented a new technique for electrical detection of single nanoparticle in a cylindrical solid-state nanopore by measuring the blockage ionic current change. Both numerical and analytical models were developed to simulate and optimize the electrical responses of the device when a single particle was passing through the nanopore. The ef...
Article
Full-text available
The analysis of single cell type typically requires expensive equipments in combination with labeling techniques. As a label-free alternative, in this letter, the characteristic dielectric properties of various cancer cell lines (MCF-7, SKOV-3, MDA-MB-231, and LnCap) and healthy peripheral blood mononuclear cells were examined and compared using th...
Patent
A particle screening device is provided. The particle screening device comprises: a substrate including a first side and a second side opposite to the first side; a micropore array formed on the substrate, wherein each micropore penetrates through the substrate from the first side to the second side and has a size configured to at least permit part...
Article
We reported a microfluidic integrated dielectrophoretic (DEP) device for single MCF-7 cell trapping and studied the different effects of applied electric field on the viability of the trapped cell. The cell remained alive when DEP voltage was 3 V. Above 3 V, cell viability significantly decreased when increasing stimulation time. At 8 V, the cell w...
Article
A sensitive and compact 3D plasmonic nanosensor was developed by deposition of gold nanoparticles (AuNPs) on a silicon micropillar array for label-free biosensing. This silicon micropillar array also worked as an autonomous capillary pump, capable of transporting a specific amount of bio-samples into the sensing area without external pumping requir...
Article
We report an on-chip nanosensor based on the localized surface plasmon resonance (LSPR) property of gold nanorings. The fabricated nanorings using nanosphere lithography showed highly tunable optical properties and were optimized to boost LSPR-based biosensing. The LSPR extinction spectra of the nanosensor agreed well with the theoretical calculati...
Article
In this report, we demonstrate a label-free genosensor based on DNA hairpins coupled to gold coated sensor surfaces. The hairpin probes were labeled with a thiolated moiety for immobilization at the 5' end and with a fluorophore for signal transduction at the 3' end. In the absence of the complement, the fluorophore is quenched by energy transfer t...
Article
The detection of small changes in the wavelength position of localized surface plasmon resonances in metal nanostructures has been used successfully in applications such as label-free detection of biomarkers. Practical implementations, however, often suffer from the large spectral width of the plasmon resonances induced by large radiative damping i...