Chen Situ

Chen Situ
Queen's University Belfast | QUB · Faculty of Medicine, Health and Life Sciences

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37
Publications
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Introduction
Skills and Expertise

Publications

Publications (37)
Article
Full-text available
Poultry farming is growing globally, particularly in developing countries, to meet the demands of growing populations for poultry meat and eggs. This is likely to lead to an increase in the use of antibiotics in poultry feed, thus contributing to the development and spread of antibiotic resistance which, poses a serious threat to human and animal h...
Article
Full-text available
The worldwide ethnobotanical use of four investigated plants indicates antibacterial properties. The aim of this study was to screen and determine significant antibacterial activity of four plant extracts in vitro and in a poultry digest model. Using broth microdilution, the concentrations at which four plant extracts inhibited Listeria monocytogen...
Article
Full-text available
Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organisms Objectives: To investigate the antimicrobial and anti-inflammatory properties of Fusarium solani et...
Poster
Governments worldwide emphasise the importance of developing alternatives in agriculture to in-feed to improve poultry health and performance whilst reducing antibiotic resistance. The aim of this research was to develop a screening process to select botanicals for in vivo study by testing the in vitro efficacy of plant extracts against bacteria...
Article
To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg-1 in swine muscle and 2.7 µg kg-1 in swine liver, which are l...
Article
Full-text available
In this paper, we report the development of a sensitive and specific monoclonal antibody-based immunodiagnostic method for the detection of iso-tenuazonic acid (ITeA), an Alternaria mycotoxin, in food samples. The ITeA was derivatized with hydrazine hydrate to produce the antigen (E)-3-(1-hydrazonoethyl)-4-hydroxy-5-isobutyl-1H-pyrrol-2(5H)-one (IT...
Article
Full-text available
To better monitor the residues of clenbuterol and salbutamol in edible tissues and products of animals treated with these compounds, a monoclonal antibody (mAb) against the β-agonists was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on this antibody was also developed. The hapten of salbutamol was synthes...
Article
Full-text available
The antimicrobial modes of action of six naturally occurring compounds, cinnamon oil, cinnamaldehyde, oregano oil, carvacrol, 2,5-dihydroxybenzaldehyde, and 2-hydroxy-5-methoxybenzaldehyde, previously found to inhibit the growth of Mycobacterium avium subsp. paratuberculosis (Map) reported to infect food animals and humans and to be present in milk...
Article
The effect of low melting fractions of milk fat on oxidative stability of ice cream was investigated. Cream was fractionated at three different temperatures (25, 15, and 10°C), designated as LF-25, LF-15, and LF-10. All the low melting fractions were individually incorporated into ice cream and compared with a control, unmodified milk fat. The othe...
Article
With the advancement of computing and optical technologies, it is now possible to capture digital holograms of real-life object scenes. Theoretically, through the analysis of a hologram, the three-dimensional (3D) objects coded on the hologram can be identified. However, being different from an optical image, a hologram is composed of complicated f...
Article
Full-text available
Monolayers composed of bacterial phospholipids were used as model membranes to study interactions of the naturally occurring phenolic compounds 2,5-dihydroxybenzaldehyde and 2-hydroxy-5-methoxybenzaldehyde, and the plant essential oil compounds carvacrol, cinnamaldehyde, and geraniol, previously found to be active against both Gram-positive and Gra...
Article
Full-text available
This study investigated the effect of ethanolic sesame cake extract on oxidative stabilization of olein based butter. Fractionation of cream was performed by the dry fractionation technique at 10 A degrees C, ethanolic sesame cake extract (SCE) was incorporated into olein butter at three different concentrations; 50, 100, 150 ppm (T-1, T-2, T-3) an...
Article
A method for fast and low bit-rate compression of digital holograms based on a new vector quantization (VQ) method known as the skip-dimension VQ (SDVQ) is proposed. Briefly, a complex hologram is converted into a real off-axis hologram, and partitioned into a set of image vectors. The image vectors are passed into a graphic processing unit (GPU),...
Article
The administration of recombinant methionyl bovine somatotropin (rMbST) to dairy cows to increase milk yield remains a common practice in many countries including the USA, Brazil, Mexico, South Africa and Korea, whereas it has been forbidden within the European Union (EU) since 1999. A rapid screening immunoanalytical method capable of the unequivo...
Conference Paper
The decision on whether a pair of closed contours is derived from different views of the same object, a task commonly known as affine invariant matching, can be encapsulated as the search for the existence of an affine transform between them. Past research has demonstrated that such search process can be effectively and swiftly accomplished with th...
Article
Chrysoidine was used as a model analyte to study its interactions with two proteins, a specific anti-chrysoidine and bovine serum albumin ( BSA) using fluorescence spectroscopy. The excitation wavelength was set at 280 nm and the emission wavelengths were scanned from 300 nm to 600 nm. The results showed that quenching mechanism of the antibody wit...
Article
Viewpoint invariant identification of fragmented scene contours can be realized by matching them against a collection of known reference models. For near planar objects, the matching of a pair of contours can be encapsulated as the search for the existence of an affine transform between them. Past research has demonstrated that the search process c...
Article
Full-text available
Built on the principle of specific biological-recognition measurements coupled with a signal transducer, surface-plasmon-resonance (SPR)-biosensor technology has developed over the past several decades, resulting in commercial production of versatile tools for many applications that greatly impact disciplines from the life sciences to the medical,...
Article
Viewpoint independent identification of fragmented object contours can be accomplished by matching them against a collection of known reference models. For the class of near-planar objects, the matching process can be posed as the search for the existence of an affine transform between a pair of contours. Recently, it has been demonstrated that the...
Article
Recently particle swarm optimization (PSO) has been successfully applied in identifying contours that are originated from different views of the same object. As compared with similar approaches based on simple genetic algorithms (SGA), the PSO exhibits higher success rates, faster convergence speed and in general more stable performance. Despite th...
Article
Coptis chinensis Franch (CCF) is commonly used in herbal remedies in Traditional Chinese Medicine (TCM) owing to its observed antimicrobial and anticancer effects in clinical medicine [1]. As an individual herb, CCF has exhibited potent inhibition on a number of bacteria including Escherichia coli in vitro conditions. The alkaloid components in CCF...
Article
The use of screening methods based on the detection of biological effects of growth promoters is a promising approach to assist residue monitoring. To reveal useful effects on protein metabolism, male and female veal calves at 10 weeks of age were treated thrice with a combination of 25mg 17beta-estradiol 3-benzoate and 150 mg 19-nortestosterone de...
Article
Full-text available
The antibacterial activities of 18 naturally occurring compounds (including essential oils and some of their isolated constituents, apple and green tea polyphenols, and other plant extracts) against three strains of Mycobacterium avium subsp. paratuberculosis (a bovine isolate [NCTC 8578], a raw-milk isolate [806R], and a human isolate [ATCC 43015]...
Article
Full-text available
Surface plasmon resonance (SPR) based biosensor technology has been widely used in life science research for many applications. While the advantages of speed, ruggedness, versatility, sensitivity and reproducibility are often quoted, many researchers have experienced severe problem of non-specific binding (NSB) to chip surfaces when performing anal...
Article
Widespread outbreaks of animal diseases have become more common, while foodborne diseases are a growing public health problem worldwide. The new European legislation banning traditional antimicrobial growth promoters has stimulated the search for suitable alternatives to chemical drugs that will help control animal diseases. Current research involv...
Article
The detection of illicit growth promoter use during meat production within the European Union is reliant on residue testing which is a limiting factor on the number of animals which can be tested and consequently compromises the efficacy of testing procedures. The present study examined a novel detection strategy based on the profiling of plasma co...
Article
Full-text available
The uses of five antimicrobial growth promoters (AGPs) zinc bacitracin, spiramycin, tylosin, virginiamycin and olaquindox in animal feedingstuffs have been prohibited in the European Union (EU) since 1999. The development and validation (in-house) of an immunoassay-based multi-analyte screening procedure for simultaneously detecting five banned AGP...
Article
A chemical confirmation method for the identification and quantification of five banned antibacterial growth promoters (AGPs) in animal feed was developed and in-house validated as part of the European Feedstuffs-RADIUS project [1]. To complete the validation process a collaborative trial was performed by eleven laboratories within the European Uni...
Article
In recent years there has been an increase in the use of tylosin in apiculture as bacterial brood diseases become resistant to oxytetracycline. Confirmatory mass spectrometry based methods have been developed but up until now there has been no complementary screening method available capable of sub 10 microg kg(-1) detection limits. In this paper t...
Article
Full-text available
As a result of increasing concerns over the transfer of resistance between different bacteria and between human and animals, a group of antimicrobial growth promoters including bacitracin, spiramycin, tylosin, virginiamycin and olaquindox have been banned in the EU since 1999. A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) with sim...
Article
The performance of a prototype multi-channel optical biosensor in both the laboratory and on site at an abattoir was evaluated. The high-throughput surface plasmon resonance (SPR) instrument allows either simultaneous analysis of eight samples for a single analyte or multi-analyte analysis. In conjunction with an automated sample pipetting station,...