Chee  How Teo

Chee How Teo
University of Malaya | UM ·  Centre For Biotechnology In Agriculture Research (CEBAR)

BSc, MSc (UPM), PhD (University of Leicester,UK)

About

87
Publications
17,209
Reads
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678
Citations
Introduction
I currently involve in transgenic plant research & molecular marker development.
Additional affiliations
September 2016 - present
University of Malaya
Position
  • Professor (Associate)
August 2014 - August 2016
Taylor's University
Position
  • Professor (Associate)
November 2012 - July 2014
Agro-Biotechnology Institute Malaysia
Agro-Biotechnology Institute Malaysia
Position
  • Research Officer
Education
September 2004 - January 2008
University of Leicester
Field of study
  • Plant biology
June 2000 - July 2002
University Putra Malaysia
Field of study
  • Molecular Biology
May 1997 - June 2000
University Putra Malaysia
Field of study
  • Biotechnology

Publications

Publications (87)
Article
Full-text available
Flooding caused or exacerbated by climate change has threatened plant growth and food production worldwide. The lack of knowledge on how crops respond and adapt to flooding stress imposes a major barrier to enhancing their productivity. Hence, understanding the flooding-responsive mechanisms of crops is indispensable for developing new flooding-tol...
Article
Full-text available
Cucumis debilis W.J.de Wilde & Duyfjes is an annual and monoecious plant. This species is endemic to Southeast Asia, particularly Vietnam. However, C. debilis is rarely studied, and no detailed information is available regarding its basic chromosome number, 45S ribosomal DNA (rDNA) status, and divergence among other Cucumis species. In this study,...
Article
Full-text available
Boesenbergia rotunda (Zingiberaceae), is a high-value culinary and ethno-medicinal plant of Southeast Asia. The rhizomes of this herb have a high flavanone and chalcone content. Here we report the genome analysis of B. rotunda together with a complete genome sequence as a hybrid assembly. B. rotunda has an estimated genome size of 2.4 Gb which is a...
Preprint
Flooding caused or exacerbated by climate change has threatened plant growth and food production worldwide. The lack of knowledge on how crops respond and adapt to flooding stress imposes a major barrier to enhancing their productivity. Hence, understanding the flooding-responsive mechanisms of crops is indispensable for developing new flooding-tol...
Preprint
Boesenbergia rotunda (Zingiberaceae), is a high-value culinary and ethno-medicinal plant of Southeast Asia. The rhizomes of this herb have high flavanone and chalcone content. Here we report genome analysis of B. rotunda together with a complete genome sequence as a hybrid assembly. B. rotunda has an estimated genome size of 2.4 Gb which was assemb...
Article
A novel glufosinate-tolerant Pseudomonas sp. LA21, was isolated from soil samples of an oil palm plantation with a long history of glufosinate application. The genome of Pseudomonas sp. LA21 was sequenced with 150 bp paired-end conducted using Illumina sequencing technology. De novo genome assembly was performed using SPAdes, ABySS, and Velvet asse...
Article
Gene prediction is a laborious and time-consuming task. The advancement of sequencing technologies and bioinformatics tools, coupled with accelerated rate of ribosome profiling and mass spectrometry development, have made identification of small open reading frames (sORFs) (< 100 codons) in various plant genomes possible. The past 50 years have see...
Article
The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibit...
Article
Full-text available
Proper identification of strain is essential in understanding the ecology of a bacteria species. The classification of Pseudomonas nitroreducens is still being questioned and revised until now. The novel P. nitroreducens strains FY43 and FY47 used in this study have been reported to show a high level of tolerance to glyphosate. In this study, next-...
Article
Full-text available
Fatmawati Y, Setiawan AB, Purwantoro A, Respatie DW, Teo CH. 2021. Analysis of genetic variability in F2 interspecific hybrids of mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system. Biodiversitas 22: 4880-4889. Mung bean (Vigna radiata L. Wilczek) categorized as one of the pivotal annual crops of Vigna genera...
Article
Full-text available
Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome...
Article
Full-text available
An unambiguous characterization of melon (Cucumis melo L.) accessions based on their morphological traits is often laborious and affected by environment when compared with molecular marker genotyping. Short interspersed nuclear elements (SINEs) are highly abundant non-autonomous and non-coding retrotransposons that are widely scattered over all chr...
Article
Full-text available
Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a neo-chromosome was formed by insertion of segments of Arabidopsis chromosomes into human chromosome 15. We used this unique system to investi...
Article
Full-text available
Mobile elements are major regulators of genome evolution through their effects on genome size and chromosome structure in higher organisms. Non-long terminal repeat (non-LTR) retrotransposons, one of the subclasses of transposons, are specifically inserted into repetitive DNA sequences. While studies on the insertion of non-LTR retrotransposons int...
Article
Global production of tomato has been hampered by the increased incidences of tomato viral disease. The high genetic heterogeneity of tomato plant viruses, because of their high mutation rates, has lead to ineffective control strategies and the fast spread of the viruses. Viruses utilize the resources in host plants for their replication. Therefore,...
Article
Full-text available
In vitro regeneration of date palm (Phoenix dactylifera L.) plants through somatic embryogenesis leads to the generation of somaclonal variants. The transposition of retrotransposons and DNA transposons in a host genome can be activated by tissue culture stresses, thus these elements can be both the cause of and useful markers for genomic variation...
Article
5-Enolpyruvylshikimate 3-phosphate synthase (EPSPS) is the primary target for the broad-spectrum herbicide, glyphosate. Improvement of EPSPS gene for high level of glyphosate tolerance is important to generate glyphosate-tolerant crops. In this study, we report the isolation and characterization of EPSPS genes of glyphosate-tolerant Pseudomonas nit...
Article
Full-text available
Transgenic technologies have been applied to a wide range of biological research. However, information on the potential epigenetic effects of transgenic technology is still lacking. Here, we show that the transgenic process can simultaneously induce both genetic and epigenetic changes in rice. We analyzed genetic, epigenetic, and phenotypic changes...
Article
Full-text available
Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft g...
Article
Full-text available
Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture Lai, Z. W., Teo, C. H. Abstract × Aims: Hyaluronic acid (HA) is a high molecular weight polymer and a major component of mucoid capsule in bacteria and extracellular matrix (ECM) of vertebrate tissue....
Article
Full-text available
Repetitive DNA sequences are highly abundant in plant genomes and are favorable probes for chromosome identification in plants. However, it is difficult to conduct studies on the details of metaphase chromosome structures in plants with small chromosomes due to their highly condensed status. Therefore, identification of homologous chromosomes for k...
Article
Full-text available
Bananas and plantains (Musa spp.) are an important socio-economic fruit crop grown worldwide. Their production across the regions where they are grown is largely hampered by pests and diseases. Fusarium wilt is a disastrous diseases of bananas caused by the fungal pathogen Fusarium oxysporum f.sp. cubense (Foc). Managing it with chemicals, biologic...
Article
Full-text available
Fusarium wilt of bananas (Musa spp.), caused by Fusarium oxysporum f. sp. cubense (Foc) causes up to 100% yield loss in bananas. Foc race 1 in particular is very devastating to dessert bananas in Uganda. One of the effective control strategies for the disease is the development of resistant cultivars through breeding. The objectives of this study w...
Article
Homologues of Secreted in Xylem (SIX) genes, a group of known fungal effectors, were here identified in the genome of Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Expression of FocSIX genes was analyzed in FocTR4 vegetative mycelia and FocTR4-infected banana roots using quantitative reverse transcription PCR (qRT-PCR). Differential g...
Article
Full-text available
Cucumis melo, one of the most important horticultural crops in Cucumis genus, has great variation in fruit sizes and shapes. Although comparative karyotype in Cucu- mis species has been conducted by several researchers, detailed information on molecular cytogenetic variation in relation to centromeres and ribosomal DNA (rDNA) loci within C. melo gr...
Article
Background Snake fruit (Salacca zalacca) is a unique tropical palm that bears fruit, botanically known as drupes, with a leathery and scaly skin that resembles snake scales. A number of studies have demonstrated that the nutritional profile of this fruit is comparable to those of better known fruits like mango, kiwi and apple, owing to its richness...
Conference Paper
The prototypic cyclotide (Kalata B1), isolated from African plant Oldenlandia affinis (Rubiceae), exhibits molluscicidal property against golden apple snails. Given their naturally high mollucicidal activities against golden apple snail, and reports on the introduction of the Oak 1 gene into various plant species, there is a possibility to generate...
Article
Full-text available
Curcuma alismatifolia widely used as an ornamental plant in Thailand and Cambodia. This species of herbaceous perennial from the Zingiberaceae family, includes cultivars with a wide range of colours and long postharvest life, and is used as an ornamental cut flower, as a potted plant, and in exterior landscapes. For further genetic improvement, how...
Article
Full-text available
Fusarium wilt of banana constantly causing considerable losses on banana industry of both local and global markets. Global exportation of banana was greatly affected by this disease since 1950s. After the emergence of a hypervirulent Tropical Race 4 strain of Fusarium oxysporum f. sp. cubense, many genetic improvements aiming to create resistant cu...
Article
Information on the abiotic stress tolerance and ice–ice disease resistance properties of tissue-cultured Kappaphycus alvarezii is scarce and can pose a big hurdle to a wider use of tissue-cultured seaweed in the industry. Here, we reported on a study of seaweed-associated bacteria diversity in farmed and tissue-cultured K. alvarezii, and ice–ice di...
Article
Full-text available
Background Detailed karyotyping using metaphase chromosomes in melon (Cucumis melo L.) remains a challenge because of their small chromosome sizes and poor stainability. Prometaphase chromosomes, which are two times longer and loosely condensed, provide a significantly better resolution for fluorescence in situ hybridization (FISH) than metaphase c...
Article
Full-text available
Minichromosomes offer an enormous potential for plant breeding and biotechnology, because they may simultaneously transfer and stably express multiple genes. Segregating independently of their host chromosomes, they provide a platform for accelerating plant breeding. Minichromosomes can be established from cloned components in vivo (bottom up) or v...
Article
Full-text available
Artificial minichromosomes are highly desir-able tools for basic research, breeding, and biotechnol-ogy purposes. We present an option to generate plant artificial minichromosomes via de novo engineering of plant centromeres in Arabidopsis thaliana by targeting kinetochore proteins to tandem repeat arrays at non-centromeric positions. We employed t...
Article
Full-text available
Engineered minichromosomes offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. Following a top-down approach, we truncated endogenous chromosomes in barley (Hordeum vulgare) by Agrobacterium-mediated transfer of T-DNA constructs containing telomere sequences....
Article
Full-text available
Minichromosomes possess functional centromeres and telomeres and thus should be stably inherited. They offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. Segregating independently of host chromosomes, they provide a platform for accelerating plant breeding. F...
Article
Full-text available
The Argonaute proteins are key components in the effector complex of RNA silencing pathways which interact with small RNAs to mediate sequence specific silencing of nucleic acid targets. In plants, these proteins are involved in a diversity of biological roles such as antiviral defence, heterochromatin regulation and in the regulation of growth and...
Article
Full-text available
Satellite DNA (satDNA) is a major component of genomes but relatively little is known about the fine-scale organization of unrelated satDNAs residing at the same chromosome location, and the sequence structure and dynamics of satDNA junctions. We studied the organization and sequence junctions of two nonhomologous satDNAs, pBuM and DBC-150, in thre...
Chapter
Full-text available
Plant pararetroviruses replicate their genome via a transcription–reverse transcription cycle like retroviruses, but unlike them their genomes do not obligatorily integrate into the host chromatin. Nevertheless, one can find complete or fragmented pararetrovirus genomes, as well as those from geminiviruses and even RNA viruses incorporated into the...
Article
We studied the structure, organization and relationship of repetitive DNA sequences in the genome of the scallop, Pecten maximus, a bivalve that is important both commercially and in marine ecology. Recombinant DNA libraries were constructed after partial digestion of genomic DNA from scallop with PstI and ApaI restriction enzymes. Clones containin...
Data
Prometaphase (A) and pachytene (B) chromosomes of S.lycopersicum "MicroTom" after fluorescent in situ hybridization with LycEPRV-Sl (red) and U30 repetitive sequence (green). The U30 signal covers most of the pericentromeric heterochromatin stained strongly with DAPI (blue) while LycEPRV-Sl has fewer hybridization sites. Bar equals 10 μm.
Article
Full-text available
Endogenous pararetroviral sequences (EPRVs) are a recently discovered class of repetitive sequences that is broadly distributed in the plant kingdom. The potential contribution of EPRVs to plant pathogenicity or, conversely, to virus resistance is just beginning to be explored. Some members of the family Solanaceae are particularly rich in EPRVs. I...
Article
Full-text available
Musa beccarii N.W.Simmonds var. beccarii and Musa beccarii N.W.Simmonds var. hottana Hakkinen were described earlier in Acta Phytotaxonomica et Geobotanica based on their morphological characteristics. In order to distinguish the genomes between the two M. beccarii varieties and M. coccinea Andrews, we have now studied them with the Inter-Retrotran...
Article
Full-text available
The banana cultivars are originated from the intra- and inter-specific hybridization of two wild diploid species, Musa acuminata Colla and Musa balbisiana Colla, contributing the A and B genomes, respectively. They are classified into genomic groups by scoring morphological features. Molecular markers provide a quick and reliable system of genome c...
Article
Full-text available
We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genusMusa. Insertional polymorphisms of the elements were studied using seven published and two newly designed primers facing outwards from the LTRs and reverse tr...
Article
Full-text available
Ty1-copia-like retrotransposons have been identified and investigated in several plant species. Here, the internal region of the reverse transcriptase (RT) gene of Ty1-copia-like retrotransposons was amplified by PCR from total genomic DNA of 10 varieties of banana. Two to four clones from each variety were sequenced. Extreme heterogeneity in the s...