Chatchai Chinpaisal

• Silpakorn University

Natural rubber (NR)'s value has become increasingly important for human applications. This study prepared NR beads as floating drug delivery system by dripping NR latex into hradening solution to obtain spherical beads. Theophylline was loaded as a model drug. NR latex formed into harden beads suddenly after dripping droplets in acidic medium. Thes...

Naturally-occurring saturated fatty acids have been classified as the interesting biomaterials. Typically, the interfacial tension and wettability relying on intermolecular forces relate to the well-fitted performance with any target sites of in situ forming matrix. This research aimed to determine surface tension/contact angle of several saturated...

Lubricant is an essential compound for pharmaceutical formulation since it is often required to ensure the success of pharmaceutical manufacturing especially tablet compression and capsule filling process. Most lubricants used in pharmaceutical industry are boundary lubricants. The most commonly used boundary lubricants are metallic salts of fatty...

Clove oil (CO) has been claimed for its efficient antimicrobial activities against many microbes especially in oral cavity and it is interesting for using as active component in the lauric acid-based solvent exchange-induced in situ forming gel (IFG). The present study investigated the physicochemical properties of lauric acid-based solvent exchang...

Background: Colorectal cancer is a common cause of cancer-related deaths Epigenetic regulation of the influx sodium dependent monocarboxylate transporter-1 )SMCT1), a tumor suppressor, was recognized in colorectal cancer. In this study, effects of Curcumin (Cur), on SMCT1 gene expression was determined. A low SMCT1 expression, HCT116, cell line was...

Tenofovir disoproxil fumarate (TDF), a nucleotide reverse transcriptase inhibitor, after conversion to TFV, is mainly eliminated by glomerular filtration and active tubular secretion. The major adverse effect of tenofovir is nephrotoxicity, however, the exact mechanism remains poorly understood. In this study, ABCC11 (MRP8) transporter, a member of...

Aims: The purposes of this study were to investigate the influence of genetic polymorphisms of cytochrome P450 2C9 (CYP2C9)FNx013 and Vitamin K epoxide reductase complex subunit 1-1639 (VKORC1-1639) G >A and patient′s characteristics on warfarin dose requirement and to establish an equation for predicting the warfarin maintenance dose in Thai patie...

The effect of aqueous and various organic extracts from the different parts of Areca catechu L. (Arecaceae) on oxidative DNA damage in human hepatocarcinoma HepG2 cells was investigated using single cell gel electrophoresis (comet) assay. Comparison of a series of organic extracts from the nut husk and different parts of this plant was performed. I...

The 360 base-pair fragment in HVS-1 of the mitochondrial genome were determined from ancient human remains excavated at Noen U-loke and Ban Lum-Khao, two Bronze and Iron Age archaeological sites in Northeastern Thailand, radio-carbon dated to circa 3,500-1,500 years BP and 3,200-2,400 years BP, respectively. These two neighboring populations were p...

The orphan nuclear receptor TR2 functions as a constitutive activator for the endogenous retinoic acid receptor beta2 (RAR(beta2)) gene expression in P19 embryonal carcinoma cells and for reporters driven by the RAR(beta2) promoter in COS-1 cells. The activation of RAR(beta2) by TR2 is mediated by the direct repeat-5 (DR5) element located in the RA...

The mouse homologue of the human receptor-interacting protein 140 (RIP140) was isolated from a mouse embryonic cDNA library in yeast two-hybrid screening experiments by using the ligand binding domain (LBD) of nuclear orphan receptor TR2 as the bait. The receptor-interacting domains of mouse RIP140 were mapped to the regions containing the LXXLL mo...

The orphan nuclear receptor TR2 and its truncated isoform deleted in the ligand binding domain (LBD) were localized exclusively in the nuclei as revealed by two methods of detection. An anti-hemagglutinin (HA) antibody detected specific nuclear localization of HA-tagged receptors and the green fluorescent protein (GFP)-tagged receptors were found t...

A unique heterodimerization pathway involving orphan receptors TR2 and TR4 is demonstrated. TR2 and TR4 preferentially form heterodimers in solution as well as on DNA elements containing a direct repeat-5 (DR5). The in vitrointeraction between TR2 and TR4 is demonstrated by the yeast and the mammalian two-hybrid interaction assays, the pull-down as...

The mouse orphan nuclear receptor TR2-11 functions as a repressor for reporter genes containing a direct repeat-5 or direct repeat-4 hormone response element. The functional domains responsible for its suppressive activity are defined, including the DNA-binding domain and the ligand-binding domain. The C-terminal 30 amino acid residues can be delet...

The mouse orphan nuclear receptor TR2-11-f suppressed the expression of reporters fused to a hormone response element of the mouse cellular retinoic acid-binding protein I gene promoter. TR2-11-f was able to bind to a direct repeat with four nucleotides in the spacer (5'TGACCTTTGGGGACCT3') located within this hormone response element as homodimers....

The mouse Rab geranylgeranyl transferase beta (Rab GGTase beta) catalytic subunit gene was isolated and characterized. This gene (Rabggtb) spans a distance of approx. 7 kb and is organized into eight exons. All the exon/intron junction sequences follow the GT/AG rule. Multiple transcription initiation sites are located within 384 bp upstream from t...

The mouse Geranylgeranyl transferase βcatalytic subunit gene has been recently isolated from a mouse E8.5 cDNA library. We now report the full length cDNA and the genomic structure of this gene. Compared to the cDNA from rat brain, the mouse GGTase β subunit has 8 extra amino acid immediately following the ATG start codon. This gene spans a distanc...

The mouse Rab geranylgeranyl transferase beta subunit has been cloned from a mouse E8.5 embryonic cDNA library. Sequence comparison reveals 97.4% sequence identity at the amino acid level to the rat clone isolated from an adult rat brain cDNA library. This gene, given a gene symbol of Rabggtb, is mapped in the distal region of mouse chromosome 3. I...

Thesis (Ph. D.)--University of Minnesota, 1998. Includes bibliographical references.