Charlotte M Vines

Charlotte M Vines
University of Texas at El Paso | UTEP · Department of Biological Sciences

Ph.D.

About

52
Publications
4,038
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1,297
Citations
Citations since 2017
14 Research Items
463 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
Additional affiliations
July 2013 - present
University of Texas at El Paso
January 2005 - June 2013
University of Kansas
January 2003 - December 2005
University of New Mexico

Publications

Publications (52)
Article
C-C chemokine receptor 7 (CCR7) plays a pivotal role in promoting the migration of T-cell acute lymphoblastic leukemia (T-ALL) cells into the central nervous system during leukemogenesis. Overexpression of CCR7 has also been linked to developing central nervous system (CNS) lymphoma. CCR7 and its ligand, CCL19 are important mediators in the develop...
Article
C-C chemokine receptor 7 (CCR7) binds to its ligand CCL19, which is expressed on the surface of activated dendritic cells, antigen activated B-cells and within lymphoid organs. Previous studies showed that homozygous deletion of CCR7 or its ligands leads to increased production of antibodies. However, it is unclear to what extent CCL19 contributes...
Article
C-C Chemokine Receptor 7 (CCR7) and its ligands (CCL19 and CCL21) are important for immune cell migration/co-localization during the primary and subsequent immune responses. We have previously shown both in humans and in mice that signaling through CCR7/CCL19 promotes rapid receptor internalization via arrestin-3. Therefore, we questioned whether i...
Article
Full-text available
C-C chemokine receptor 7 (CCR7) was one of the first two chemokine receptors that were found to be upregulated in breast cancers. Chemokine receptors promote chemotaxis of cells and tissue organization. Since under homeostatic conditions, CCR7 promotes migration of immune cells to lymph nodes, questions immediately arose regarding the ability of CC...
Article
Full-text available
Background: Breast cancer (BC) continues to have the second highest mortality amongst women in the United States after lung cancer. For 2021, the American Cancer Association predicted 281,550 new invasive breast cancer cases besides 49,290 new cases of non-invasive breast cancer and 43,600 deaths from the metastatic disease. A treatment modality i...
Article
Full-text available
The potential emergence of SARS-CoV-2 variants capable of escaping vaccine-generated immune responses poses a looming threat to vaccination efforts and will likely prolong the duration of the COVID-19 pandemic. Additionally, the prevalence of beta coronaviruses circulating in animals and the precedent they have set in jumping into human populations...
Article
It has become increasingly evident that the bone marrow is an important niche for memory B cells and memory T cells that can be activated to become effector T cells during a secondary immune response. However, signaling events that promote bone marrow persistence of memory cells remain largely unknown. We have found that the C-C chemokine receptor...
Article
Ligand mediated GPCR internalization/signaling is regulated by G protein-coupled receptor kinase (GRK) induced receptor phosphorylation and recruitment of β arrestins. The GPCR chemokine receptor, CCR7, has two ligands, CCL19 and CCL21 that mediate chemotactic migration of CCR7 expressing immune cells, including naïve T cells. Distinct internalizat...
Chapter
Full-text available
Phospholipase C (PLC) family members constitute a family of diverse enzymes. Thirteen different family members have been cloned. These family members have unique structures that mediate various functions. Although PLC family members all appear to signal through the bi-products of cleaving phospholipids, it is clear that each family member, and at t...
Article
Full-text available
Chronic low-level lead exposure alters cognitive function in young children however the mechanisms mediating these deficits in the brain are not known. Previous studies in our laboratory showed that early lead exposure reduced the number of microglial cells in hippocampus/dentate gyrus of C57BL/6 J mice. In the current study, C-C chemokine receptor...
Article
G protein-coupled receptor (GPCR) signaling can be regulated by phosphorylation via GPCR kinases (GRKs) and recruitment of b-arrestins. The C-C chemokine receptor 7 (CCR7) GPCR, has two ligands, CCL19 and CCL21 that promote chemotactic migration of CCR7 expressing immune cells, including naïve T cells. Distinct signaling pathways are activated in T...
Research
Full-text available
In casual conversation with non-medical individuals, it is common for them to ask: why do we not have a cure for cancer or vaccinations for all diseases? It seems somewhat logical to assume that after so many years of research that cures should be readily available, diseases in general should simply require a pill or jab and that somehow, if scient...
Article
Full-text available
Although IL-17 is emerging as an important cytokine in cancer promotion and progression, the underlining molecular mechanism remains unclear. Previous studies suggest that IL-17 (IL-17A) sustains a chronic inflammatory microenvironment that favors tumor formation. Here we report a novel IL-17-mediated cascade via the IL-17R-Act1-TRAF4-MEKK3-ERK5 po...
Chapter
Phospholipases play central roles in regulating signalling during numerous cellular events including proliferation, G protein-coupled receptor signalling and neuronal activation by catalysing the lysis of phosphorylated lipids. In turn, second messengers are released, which control downstream events. Four distinct families of phospholipases have be...
Article
Full-text available
Autoimmune diseases such as multiple sclerosis (MS) are characterized by the breakdown of immune tolerance to autoantigens. Targeting surface receptors on immune cells offers a unique strategy for reprogramming immune responses in autoimmune diseases. The B7 signaling pathway was targeted using adaptations of soluble antigen array (SAgA) technology...
Article
Full-text available
Aim: Approximately 30 percent of pediatric T-cell acute lymphoblastic leukemia (T-ALL) patients who undergo standard chemotherapy will suffer disease relapse within two years of completing treatment. Many of these patients develop leukemic involvement of the central nervous system (CNS). Currently, there is an urgent need to identify chemotherapeut...
Article
Full-text available
Autoimmune diseases such as multiple sclerosis (MS) are typified by the misrecognition of self-antigen and the clonal expansion of autoreactive T cells. Antigen-specific immunotherapies (antigen-SITs) have long been explored as a means to desensitize patients to offending self-antigen(s) with the potential to retolerize the immune response. Soluble...
Chapter
Phospholipase C (PLC) family members play critical roles in regulating immune cell functions during inflammatory responses. This chapter discusses how different family members can be activated by G-protein coupled receptors, T-cell receptors, B-cell receptors, and other tyrosine kinase receptors, in addition to many of the pathways that contribute...
Article
Full-text available
A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic ce...
Article
Functional loss of C-C chemokine receptor 7 (CCR7) and its ligands, CCL19 and CCL21, has been linked to development of autoimmune diseases. Therefore, we hypothesized that CCR7 antagonists could be used to promote an immune response that could be measured by tittering antibodies produced in animals injected with test antigens. To test this hypothes...
Article
Full-text available
Phospholipase C (PLC) family members constitute a family of diverse enzymes. Thirteen different family members have been cloned. These family members have unique structures that mediate diverse functions. Although PLC family members all appear to signal through the bi-products of cleaving phospholipids, it is clear that each family member, and at t...
Article
Full-text available
T lymphocytes circulate between the blood, tissues, and lymph. These T cells carry out immune functions, using the C-C chemokine receptor 7 (CCR7) and its cognate ligands, CCL19 and CCL21, to enter and travel through the lymph nodes. Distinct roles for each ligand in regulating T lymphocyte trafficking have remained elusive. We report that in the h...
Article
The efficacy of bifunctional peptide inhibitor (BPI) in preventing blood-brain barrier (BBB) breakdown during onset of experimental autoimmune encephalomyelitis (EAE) and suppression of the disease was evaluated in mice. The mechanism that defines how BPI prevents the disease was investigated by measuring the in vitro cytokine production of splenoc...
Article
Full-text available
Proteins participating in immunological signaling have emerged as important targets for controlling the immune response. A multitude of receptor-ligand pairs that regulate signaling pathways of the immune response have been identified. In the complex milieu of immune signaling, therapeutic agents targeting mediators of cellular signaling often eith...
Article
Dendritic cells (DCs) are potent professional antigen presenting cells (APC) that activate naïve T cells. Interaction of ICAM-1 and LFA-1 molecules on each cell is required for T cell conjugation to DCs, which leads to naïve CD4+ T cell activation and proliferation. Nanoparticles capable of blocking LFA-1/ICAM-1 interaction were studied as inhibito...
Article
Full-text available
C-C chemokine receptor 7 (CCR7) controls lymphocyte migration to secondary lymphoid organs. Although CCR7 has been implicated in targeting the metastasis of cancers to lymph nodes, the role of CCR7 in the metastasis of breast cancer, along with the molecular mechanisms that are controlled by CCR7 that target breast cancer metastasis to the lymph no...
Article
Full-text available
CCR7 binds to its cognate ligand, CCL21, to mediate the migration of circulating naive T lymphocytes to the lymph nodes. T lymphocytes can bind to fibronectin, a constituent of lymph nodes, via their β1 integrins, which is a primary mechanism of T lymphocyte migration; however, the signaling pathways involved are unclear. We report that rapid (with...
Article
C-C Chemokine Receptor 7 (CCR7) regulates migration of naïve T cells to lymph nodes, via chemotaxis to the ligand, CCL21. Approximately 72 hours after lymph node entry, Endothelial Differentiation Gene 1 (EDG-1) is up-regulated on T cells and mediates lymph node egress. It is unclear how EDG-1 expression is up-regulated. We hypothesized that lymph...
Article
Full-text available
Internalization of ligand bound G protein-coupled receptors, an important cellular function that mediates receptor desensitization, takes place via distinct pathways, which are often unique for each receptor. The C-C chemokine receptor (CCR7) G protein-coupled receptor is expressed on naive T cells, dendritic cells, and NK cells and has two endogen...
Article
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or mo...
Article
Recent studies have highlighted the emergence of a class of G protein-coupled receptors that are internalized in an arrestin-independent manner. In addition to demonstrating that the N-formyl peptide receptor belongs in this family, we have recently shown that recycling of the receptor requires the presence of arrestins. To further elucidate mechan...
Article
Full-text available
In response to ligand binding, G protein-coupled receptors undergo phosphorylation and activate cellular internalization machinery. An important component of this process is the concentration of receptors into clusters on the plasma membrane. Aside from organizing the receptor in anticipation of internalization, little is known of the function of l...
Article
Mast cells and neutrophils play a major role in the innate immune response. Following invasion of the host by microorganisms, these immune cells become activated and release anti-microbial cytotoxic granules in an effort to destroy invading microorganisms in a process termed degranulation. By-products from the degradation of microorganisms can also...
Article
Full-text available
G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptors (GPCRs) activate numerous cellular signals through the combined actions of G proteins, GPCR kinases, and arrestins. Although arrestins have traditionally been thought of as mediating GPCR desensitization, they have now been shown to play important roles in the internali...
Article
Full-text available
Arrestins mediate phosphorylation-dependent desensitization, internalization, and initiation of signaling cascades for the majority of G protein-coupled receptors (GPCRs). Many GPCRs undergo agonist-mediated internalization through arrestin-dependent mechanisms, wherein arrestin serves as an adapter between the receptor and endocytic proteins. To u...
Article
Full-text available
One of the major functions of the N-formyl peptide receptor (FPR) is to mediate leukocyte degranulation. Phosphorylation of the C-terminal domain of the FPR is required for receptor internalization and desensitization. Although arrestins mediate phosphorylation-dependent desensitization, internalization, and initiation of novel signaling cascades f...
Article
While beta 2 integrin ligand-receptor recognition interactions are well characterized, less is known about how these events trigger signal transduction cascades to regulate the transition from tethering to firm adhesion, spreading, and transendothelial migration. We have identified critical positive and negative regulatory components of this cascad...
Article
Full-text available
Ingestion of opsonized pathogens by professional phagocytes results in the generation and release of microbicidal products that are essential for normal host defense. Because these products can result in significant tissue injury, phagocytosis must be regulated to limit damage to the host while allowing for optimal clearance and destruction of opso...
Article
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The effect of the maturation-inducing polar solvent, hexamethylene bisacetamide (HMBA), on the radiosensitivity of two human tumour cell lines (clone A, a colon carcinoma; and EJ, a bladder carcinoma) was investigated. Exposure of clone A or EJ cells to HMBA resulted in a concentration-dependent increase in doubling time, a decreased plating effici...
Article
The differentiation-inducing agent N-methylformamide (NMF) enhances the sensitivity of some cell lines to ionizing radiation. To elucidate the mechanism of NMF-mediated radiosensitization, we examined the effects of this agent on gamma-ray-induced DNA double-strand breaks and micronuclei in two cell lines, clone A (human colon carcinoma) and HCA-1...
Article
Full-text available
The ability of the sister chromatid exchange (SCE) assay to detect heterogeneity in intrinsic radiation sensitivity was investigated. In order to identify tumour cell subpopulations, frequency histograms of cis-diamminedichloroplatinum (II) (cPt)-induced SCEs were generated and compared to those from cultures that had been irradiated 96 h before dr...
Article
Full-text available
Exposure of certain cell lines to the differentiation-inducing agent N-methylformamide (NMF) enhances their radiosensitivity. As part of an attempt to elucidate the mechanism of NMF-induced radiosensitization, we examined the effects of NMF on chromatin structure, as reflected by changes in DNA-protein cross-links (DPCs) and the chromatin protein/D...
Article
Full-text available
The effects of the differentiation-inducing polar solvent N-methylformamide (NMF) on artificially induced and spontaneous metastases from a murine hepatocarcinoma (HCA-1) in C3Hf/Kam mice were investigated. Exposure of HCA-1 cells in vitro for 6 days to 1.0% or 1.25% NMF resulted in an increase in the number of lung nodules formed in mice when thes...
Article
Full-text available
The effect of cis-diamminedichloroplatinum(II) (cPt) on sister chromatid exchange (SCE) induction was determined in 13 human primary tumor cell cultures. Primary cultures were derived from surgical specimens of solid tumors composed of a variety of histologies. Three to 16 days after biopsy, depending on the growth rate, cultures were treated with...
Article
Full-text available
The limitations of the agar suspension culture method for primary culturing of human tumor cells prompted development of a monolayer system optimized for cell adhesion and growth. This method grew 83% of fresh human tumor cell biopsy specimens, cultured and not contaminated, from a heterogeneous group of 396 tumors including lung cancer (93 of 114,...
Article
The effects of the differentiation-inducing polar solvent N-methylformamide (NMF) on the in vitro response of murine hepatocarcinoma (HCa-1) cells to 1,3-bis(2-chloroethyl)-1-nitrosourea, cis-diamminedichloroplatinum (II), and melphalan were investigated using the sister chromatid exchange (SCE) and cell survival assays. When cells were exposed to...
Article
The effects of the differentiation-inducing polar solvent dimethylsulfoxide (DMSO) on the in vitro response of murine hepatocarcinoma cells to cisplatinum, BCNU, and melphalan were investigated using the sister chromatid exchange (SCE) and cell survival assays. Growth of cells in medium containing 2 per cent DMSO enhanced drug-induced SCEs and cell...

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