Charlotte Nef

Charlotte Nef
Ecole Normale Supérieure de Paris | ENS · Institut de Biologie de l’Ecole Normale Supérieure (IBENS)

Ph.D

About

13
Publications
2,620
Reads
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46
Citations
Introduction
I am a post-doctoral researcher in marine biology and ecology. I study interactions between organisms and how biotic and abiotic factors can modify their interactions. I combine molecular methods (gene expression analyses, comparative proteomics) with classical physiological approach (biochemical measurements, controlled cultures) and computing biology (network analyses). Besides research, I like to be involved in scientific education.
Additional affiliations
February 2016 - July 2016
Institut Français de Recherche pour l'Exploitation de la Mer
Position
  • Master's Student
Description
  • Study on the influence of light and nutrition on the growth and toxin production of Dinophysis acuminata and D. sacculus
April 2015 - June 2015
University of Nantes
Position
  • MS internship
Description
  • Study on the retention time of chloroplasts and microalgae by the Sacoglossan Elysia viridis and the Nudibranchs Spurilla neapolitana and Aeolidia papillosa
July 2014 - July 2014
University of Nantes
Position
  • BS internship to discover research topics
Description
  • Participation to the analysis of meiofauna samples, identification and taxonomy of bivalves, estimation of oyster mortality, sample preparation for SMRI analysis

Publications

Publications (13)
Article
Full-text available
Haptophyte microalgae are key contributors to microbial communities in many environments. It has been proposed recently that members of this group would be virtually all dependent on vitamin B12 (cobalamin), an enzymatic cofactor produced only by some bacteria and archaea. Here, we examined the processes of vitamin B12 acquisition by haptophytes. W...
Preprint
Full-text available
Diatoms form a diverse and abundant group of photosynthetic protists that are essential players in marine ecosystems. However, the microevolutionary structure of their populations remains poorly understood, particularly in polar regions. Exploring how closely related diatoms adapt to different oceanic ecoregions is essential given their short gener...
Chapter
Almost half of photosynthesis on Earth occurs in marine and freshwater environments; hence, aquatic photosynthetic organisms have an enormous impact on food webs and on the biogeochemistry of the entire Earth System. In this chapter, the authors provide an overview of the main groups of aquatic photoautotrophs, exploring their complex evolutionary...
Preprint
Full-text available
The Arctic Ocean is being impacted by warming temperatures, increasing freshwater and highly variable ice conditions. The microalgal communities underpinning Arctic marine food webs, once thought to be dominated by diatoms, include a phylogenetically diverse range of small algal species, whose biology remains poorly understood. Here, we present gen...
Article
Full-text available
Marine plankton form complex communities of interacting organisms at the base of the food web, which sustain oceanic biogeochemical cycles and help regulate climate. Although global surveys are starting to reveal ecological drivers underlying planktonic community structure and predicted climate change responses, it is unclear how community-scale sp...
Article
Diatoms, an evolutionarily successful group of microalgae, display high levels of intraspecific genetic variability in natural populations. However, the contribution of various mechanisms generating such diversity is unknown. Here we estimated the genetic micro-diversity within a natural diatom population and mapped the genomic changes arising with...
Preprint
Full-text available
Marine plankton form complex communities of interacting organisms at the base of the food web, which sustain oceanic biogeochemical cycles, and help regulate climate. Though global surveys are starting to reveal ecological drivers underlying planktonic community structure, and predicted climate change responses, it is unclear how community-scale sp...
Preprint
Full-text available
Diatoms, an evolutionarily successful group of microalgae, display high levels of intraspecific variability in natural populations. However, the process generating such diversity is unknown. Here we estimated the variability within a natural diatom population and subsequently mapped the genomic changes arising within cultures clonally propagated fr...
Article
Full-text available
Cobalamin (vitamin B12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, not...
Article
Full-text available
Vitamin B12 (cobalamin) can control phytoplankton development and community composition, with around half of microalgal species requiring this vitamin for growth. B12 dependency is determined by the absence of cobalamin-independent methionine synthase and is unrelated across lineages. Despite their important role in carbon and sulphur biogeochemist...
Poster
Full-text available
Vitamin B12, or cobalamin, is an enzymatic cofactor synthetized only by some prokaryotes that can limit phytoplankton growth in several oceanic regions. It is assumed that around half of microalgae species are B12-dependent (auxotrophic) for their development with no clear phylogenetic pattern. We investigated B12 auxotrophy of Haptophyta lineage...
Poster
Full-text available
This work aimed to characterize the global metabolic response of the B12-dependent and oleaginous microalgae Tisochrysis lutea during B12 limitation and repletion.
Poster
Full-text available
This poster sums up the different steps of selecting vitamin B12-producing bactera in order to cultivate the B12-auxotroph microalgae Tisochrysis lutea. This work is part of the project about microalgae-bacteria interactions for nutrients.

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Cited By

Projects

Projects (2)
Project
- Analysis of vitamin B12-dependency of Tisochrysis lutea. - Set up and validation of an extraction & dosage protocole of vitamin B12. - Purification of microalgae cultures. - Bioinformatic research about B12-dependent proteins in T. lutea to reconstruct its B12 metabolism. - Metabolic response analysis during B12 limitation in continuous culture. - Screening & selection of B12-producing bacteria to cultivate T. lutea without B12 input, and analysis of their interactions.