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Charlotte Francoeur

Charlotte Francoeur
Rutgers University - Newark · Earth and Environmental Sciences

Doctor of Philosophy

About

6
Publications
1,392
Reads
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63
Citations
Citations since 2017
5 Research Items
62 Citations
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Education
August 2016 - March 2022
University of Wisconsin–Madison
Field of study
  • Insect-Microbe Symbiosis
August 2012 - May 2016
University of Maryland, College Park
Field of study
  • Microbiology

Publications

Publications (6)
Article
Full-text available
Fungi shape the diversity of life. Characterizing the evolution of fungi is critical to understanding symbiotic associations across kingdoms. In this study, we investigate the genomic and metabolomic diversity of the genus Escovopsis, a specialized parasite of fungus-growing ant gardens. Based on 25 high-quality draft genomes, we show that Escovops...
Article
Within animal associated microbiomes, the functional roles of specific microbial taxa are often uncharacterized. Here, we use the fungus-growing ant system, a model for microbial symbiosis, to determine the potential defensive roles of key bacterial taxa present in the ants’ fungus gardens. Fungus gardens serve as an external digestive system for t...
Article
The hyper‐diverse order Coleoptera comprises a staggering ~25% of known species on Earth. Despite recent breakthroughs in next generation sequencing, there remains a limited representation of beetle diversity in assembled genomes. Most notably, the ground beetle family Carabidae, comprising more than 40,000 described species, has not been studied i...
Article
Full-text available
Leaf-cutter ants are dominant neotropical herbivores capable of deriving energy from a wide range of plant substrates. The success of leaf-cutter ants is largely due to their external gut, composed of key microbial symbionts, specifically, the fungal mutualist L. gongylophorus and a consistent bacterial community. Both symbionts are known to have c...

Questions

Question (1)
Question
I have been having trouble with some yellow fluorescence occurring while using DAPI.
Image 1: DAPI staining of fungal nuclei lysed from hyphae. I took 20uL samples and added 1uL of 1mg/mL DAPI.
Image 2: DAPI staining of fungal nuclei inside hyphae. The hyphae were collected from a rich nutrient broth so I was thinking maybe the DAPI was reacting with an unwashed component of that?

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