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Publications
Publications (137)
The use of in vitro systems to investigate the process of corneal wound healing offers the opportunity to reduce animal pain inflicted during in vivo experimentation. This study aimed to establish an easy-to-handle ex vivo organ culture model with porcine corneas for the evaluation and modulation of epithelial wound healing. Cultured free-floating...
Background/aims:
The use of skin-derived stem cells and stem cells of other origins in regenerative medicine requires knowledge of stem cell fate after transplantation. In order to achieve non-invasive long-term imaging and tracking of transplanted stem cells in preclinical studies, a non-toxic, efficient labeling technique that does not alter ste...
The combination of an aging population and an increasing prevalence of diseases associated with impaired-wound healing, including obesity, peripheral vascular disease and diabetes, is likely to result in a dramatic increase in the incidence and prevalence of chronic skin wounds. Indeed, systemic reviews are now not only trying to establish both the...
High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded i...
Aqueous tear deficiency due to lacrimal gland insufficiency is one of the major causes of dry eye disease. In severe cases, such as Sjogren's syndrome, Stevens-Johnson syndrome, or ocular cicatricial pemphigoid, therapy with artificial tears is often insufficient to relieve severe discomfort, prevent progressive ocular surface disease, or enable vi...
The invention relates to material compositions comprising adult stem cells obtained from exocrine gland tissue and a supporting matrix having the shape of a thread structure and/or of a net. The supporting matrix preferably consists of a plastic material which is physiologically acceptable and degradable in the body. The material compositions of th...
A targeted cultivation phase adjustment is provided in a process for the cultivation of cells in which biological cells are cultivated on at least one boundary surface between different, non-solid cultivation phases so that pre-determined cultivation conditions are given. A cultivation apparatus (100) for biological cells is also provided that incl...
The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comp...
Human skin harbours multiple different stem cell populations. In contrast to the relatively well-characterized niches of epidermal and hair follicle stem cells, the localization and niches of stem cells in other human skin compartments are as yet insufficiently investigated. Previously, we had shown in a pilot study that human sweat gland stroma co...
A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the subst...
With the increasing relevance of cell-based therapies, there is a demand for cell-labeling techniques for in vitro and in vivo studies. For the reasonable tracking of transplanted stem cells in animal models, the usage of quantum dots (QDs) for sensitive cellular imaging has major advances. QDs could be delivered to the cytoplasm of the cells provi...
A long term functional and reliable coupling between neural tissue and implanted microelectrodes is the key issue in acquiring neural electrophysiological signals or therapeutically excite neural tissue. The currently often used rigid micro-electrodes are thought to cause a severe foreign body reaction resulting in a thick glial scar and consequent...
Nestin-expressing cells isolated from different human tissues reveal self-renewal capacity and a multilineage differentiation potential. In particular adult stem/progenitor cell populations from exocrine glands like pancreas, salivary gland and sweat gland are characterized by a prominent nestin expression. Interestingly human mammary gland histolo...
Mammalian and fish skin share protective activities against environments that are rich in infectious agents. Fish epidermis is endowed with an extrinsic barrier consisting of a mucus layer and antimicrobial peptides (AMPs). These operate together as a protective chemical shield. As these AMPs are evolutionarily well preserved and also found in high...
Cell based therapies promise to be a cure for many incurable diseases.
However, despite extensive investments in the last two decades, most
cell-based therapies have failed to deliver clinical and financial success.
Particularly, autologous products are still too expensive compared to
conventional therapies. We want to report on a newly developed a...
The current quest for brain-machine-interfaces is hindered by the need to find a reliable solution for truly chronic brain implants. One approach to tackle that problem is not only to use flexible, mechanically compliant micro recording probes, but to enhance their acceptance in the target tissue by biologization. In order to shed light on the best...
Background:
Pancreatic cancer is one of the most deadly malignancies with insufficient therapeutic options and poor outcome. Cancer stem cells (CSCs) are thought to be responsible for progression and therapy resistance. We investigated the potential of pancreatic cell lines for CSC research by analyzing to what extent they contain CSC populations...
3-dimensional fish cell culture systems are advantageous tools to study e.g. cell interactions, cell differentiation and tissue formation. However, cultivation of 3-dimensional cultures is difficult. A lot more has to be learned about the features that allow cultivation of tissue nnnnnn-like structures, after all about the interplay of different ce...
Vascularization is a key process in tissue engineering and regeneration and represents one of the most important issues in the field of regenerative medicine. Thus, several strategies to improve vascularization are currently under clinical evaluation. In this study, stem cells derived from human sweat glands were isolated, characterized, seeded in...
Background: Beside its anti-proliferative, anti-hypertensive and anti-inflammatory effects, heparin has shown the apoptotic effect in lymphoblasts. In the study, it is aimed to show the apoptotic effect of heparin in lymphoblasts with measuring intracellular calcium and using DNA analysis by flow cytometry, in vitro. Methods: Twenty-three newly dia...
To establish and maintain a cross generational cryogenic collection of rare animal biomaterial resources, several technological precautions need to be considered to ensure the usability of the stored samples at longterm. In the German Cell Bank for Wildlife "Alfred Brehm" (short: "CRYO-BREHM") different biotechnological and cryotechnological featur...
We describe a method for fast and easy isolation of cells via trypsin digestion from larvae of Atlantic sturgeon Acipenser oxyrinchus oxyrinchus resulting in a stable, well-proliferating cell culture. The culture conditions for these cells were optimized with the aim of supporting the production of high amounts of biomass. To enhance cell growth an...
Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We fur...
Human dermal fibroblast (HDF) (data set D) were cultured over more than 10 days and imaged at four positions. Analogous to the figure S1, the proliferation curves are shown in (A), the changes of confluency in (B,C) and the change of mean cell area in (D).
(TIFF)
A culture of proliferating PSCs is shown during a time lapse experiment using oblique illumination. This image sequence was used to create reference data set B.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 1 / 10 is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 3 / 10 is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 5 / 10 is shown.
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This video shows the proliferation of PSCs during a timelapse experiment. Video / position 8 / 10 is shown.
(AVI)
Selected previous tracking work, compared to one another with respect to important technical details. The tracking tasks and the methods of evaluation are very diverse, making a direct comparison rather difficult.
(DOC)
A culture of proliferating PSCs is shown during a time lapse experiment using phase contrast microscopy. This image sequence belongs to reference data set B.
(AVI)
The complete genealogical tree for one cell is shown. This tree was reconstructed using the reference data set B.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 4 / 10 is shown.
(AVI)
Pancreatic stem cells (PSCs, data set C) in passage 31 were cultured and observed over a time period of three days and imaged at 10 different positions. The proliferation curves are shown in (A), the changes of confluency in (B,C). The last subfigure (D) displays the change of mean cell area of these different positions. The variances between the 1...
A culture of proliferating PSCs is shown during a time lapse experiment using oblique illumination. This image sequence was used to create reference data set A.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 7 / 10 is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 9 / 10 of data set D is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 10 / 10 is shown.
(AVI)
This videos shows the proliferation of HDFs during a time lapse experiment. Video / Position 1 / 4 is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 2 / 10 is shown.
(AVI)
This video shows the proliferation of PSCs during a timelapse experiment. Video / position 6 / 10 is shown.
(AVI)
This videos shows the proliferation of HDFs during a time lapse experiment. Video / Position 3 / 4 is shown.
(AVI)
This videos shows the proliferation of HDFs during a time lapse experiment. Video / Position 4 / 4 is shown.
(AVI)
This videos shows the proliferation of HDFs during a time lapse experiment. Video / Position 2 / 4 is shown.
(AVI)
Automated microscopy is currently the only method to non-invasively and label-free observe complex multi-cellular processes, such as cell migration, cell cycle, and cell differentiation. Extracting biological information from a time-series of micrographs requires each cell to be recognized and followed through sequential microscopic snapshots. Alth...
Minimizing the foreign body response is seen as one critical research strategy for implants especially when designed for immune-privileged organs like the brain. The context of this work is to improve deep brain stimulating devices used in a consistently growing spectrum of psychomotor and psychiatric diseases mainly in form of stiff electrodes. Ba...
Targeted differentiation of stem cells is mainly achieved by the sequential administration of defined growth factors and cytokines, although these approaches are quite artificial, cost-intensive and time-consuming. We now present a simple xenogeneic rat brain co-culture system which supports neuronal differentiation of adult human stem cells under...
We report the isolation, cultivation, and cryopreservation of cells from adipopancreatic tissue of adult Atlantic herring Clupea harengus. The cell population was cultured for >1 yr and had a mean doubling time of 2 wk. Immunocytochemical analyses revealed that >50% of cells contained glial fibrillary acidic protein and pan-cytokeratins. Vigilin, a...
Cellular models are an interesting tool to study human heart diseases. To date, research groups mainly focus on mouse models, but important murine physiology is different from human characteristics. Recently, scientists found that the electrophysiology of fish cardiomyocytes largely resembles that of humans. So far, cardiomyocyte models were genera...
Adult stem cells are characterized by the ability to maintain their population through cell division and concomitantly generate specialized somatic cell types through differentiation. Stem cells generated from the adult pancreas proliferate rapidly in vitro and are able to develop into cell types of all three germ layers Kruse et al. (2004). Theref...
Background: Applying stem cell therapy in a failing myocardium, the dimension of an intra-myocardial cell homing is significant. Thus a comparison of the homing potential between glandular (GSCs) and mesenchymal stem cells (MSCs) was performed within the myocardium of a big animal model. Methods: In African Bore Goats the intramyocardial homing and...
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Please cite this paper as: An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis. Experimental Dermatology 2010; 19: 549–555.
Abstract: Human skin-derived Nestin+ cells serve as a convenient source for autologous, adult, pluripotent progenitor cells that o...
Cellular replacement therapies in vascular and urogenital organ disorders require an abundant source of smooth muscle cells. A promising approach would be the directed myogenic differentiation (characterized by the expression of alpha-smooth muscle actin (alpha-SMA)) into a sufficient amount of smooth muscle cells through easily obtainable adult st...
Please cite this paper as:‘Fish matters’: the relevance of fish skin biology to investigative dermatology. Experimental Dermatology 2010.
Abstract: Fish skin is a multi-purpose tissue that serves numerous vital functions including chemical and physical protection, sensory activity, behavioural purposes or hormone metabolism. Further, it is an impor...
In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss, the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presen...
Clinical success in tissue regeneration requires improvements in vascularization capacity of scaffolds. Several efforts have been made in this field including cellular and acellular technologies. In this work we combined the use of stem cells derived from pancreas or submandibular glands expressing green fluorescent protein (GFP(+)) with a commerci...
We have recently shown that the expression of nestin, a progenitor/stem cell marker protein, is localized in different mesenchymal compartments in human skin including the sweat gland stroma.
As other exocrine glands are recognized sources of multipotent stem cell populations with potential for multilineage differentiation, it was our aim to isolat...
Stem cells derived from pancreatic tissue are well characterized and exhibit a broad plasticity as they can differentiate beyond lineage boundaries into many cell types. The aim of this study was the comparative characterization of pancreatic stem cells with one other derivate of the embryonic foregut, namely salivary glands, for the existence of s...
Several research groups have reported on the existence and in vitro characterization of multipotent stem-cells from the pancreas. However, the origin of these cells remains largely unexplained. Here, we report that in vitro culturing itself can turn adult cells from human exocrine pancreas into a cell population with typical stem cell characteristi...
Cutaneous nestin+ cells are of substantial interest in regenerative medicine. However, the location of nestin+ cells in situ remains controversial. We therefore sought to determine their location in female human scalp skin, using stringently controlled immunohistochemical techniques, Western blot analysis, and in situ hybridization and complementin...