Charles Ramassamy

Charles Ramassamy
Institut Pasteur International Network · INRS - Institut Armand-Frappier

About

133
Publications
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5,413
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Publications

Publications (133)
Article
Full-text available
Here, we propose a new theoretical framework that enables the use of Differential Dynamic Microscopy (DDM) in fluorescence imaging mode to quantify in situ protein adsorption onto nanoparticles (NP) while simultaneously monitoring for NP aggregation. We use this methodology to elucidate the thermodynamic and kinetic properties of the protein corona...
Article
Full-text available
The overall impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on our society is unprecedented. The identification of small natural ligands that could prevent the entry and/or replication of the coronavirus remains a pertinent approach to fight the coronavirus disease (COVID-19) pandemic. Previously, we showed that the phenolic...
Preprint
Unravelling the mystery of Alzheimer Disease (AD) requires urgent resolution given the worldwide increase of the aging population. There is a growing concern that the current leading AD hypothesis, the amyloid cascade hypothesis, does not stand up to validation with respect to emerging new data. Indeed, several paradoxes are being discussed in the...
Article
In brain, extracellular vesicles (EVs) play an essential role in neuron‐glia interface and ensure the crosstalk between the brain and the periphery. Only a few studies have demonstrated the apolipoprotein E4 variant (APOE ε4)‐driven dysfunction of EVs pathway and the risk to develop Alzheimer’s disease (AD). To better understand the role of APOE ε4...
Article
Background: Growing evidence supports that receptor for advanced glycation end products (RAGE) and glyoxalase-1 (GLO-1) are implicated in the pathophysiology of Alzheimer's disease (AD). Extracellular vesicles (EVs) are nanovesicles secreted by almost all cell types, contribute to cellular communication, and are implicated in AD pathology. Recentl...
Article
Full-text available
Introduction: The substantial link between apolipoprotein E (APOE) ε4 allele and oxidative stress may underlie enhanced Alzheimer's disease (AD) risk. Here, we studied the impact of APOE ε4 on the level of apolipoproteins with antioxidant activities along with oxidative markers in circulating extracellular vesicles (cEVs) and plasma from cognitive...
Article
Zwitterion polymers with strong antifouling properties have been suggested as the prime alternative to polyethylene glycol (PEG) for drug nanocarriers surface coating. It is believed that PEG coating shortcomings, such as immune responses and incomplete protein repellency, could be overcome by zwitterionic polymers. However, no systematic study has...
Article
Full-text available
The COVID-19 disease caused by the virus SARS-CoV-2, first detected in December 2019, is still emerging through virus mutations. Although almost under control in some countries due to effective vaccines that are mitigating the worldwide pandemic, the urgency to develop additional vaccines and therapeutic treatments is imperative. In this work, the...
Article
Full-text available
Background Alzheimer’s disease (AD) is the most common neurodegenerative disease ultimately manifesting as clinical dementia. Despite considerable effort and ample experimental data, the role of neuroinflammation related to systemic inflammation is still unsettled. While the implication of microglia is well recognized, the exact contribution of per...
Article
Full-text available
Alzheimer’s disease (AD) is the most common age-related neurodegenerative disorder, responsible for nearly two-thirds of all dementia cases. In this review, we report the potential AD treatment strategies focusing on natural polyphenol molecules (green chemistry) and more specifically on the inhibition of polyphenol-induced amyloid aggregation/disa...
Article
Significance: Glutathione (GSH) represents the most abundant and the main antioxidant in the body with important functions in the brain related to Alzheimer's disease (AD). Recent Advances: Oxidative stress is one of the central mechanisms in AD. We and others have demonstrated the alteration of GSH in AD brain, its important role for the detoxifi...
Article
Introduction: In brain, extracellular vesicles (EVs) play an essential role in the neuron-glia interface and ensure the crosstalk between the brain and the periphery. Some studies now link the pathway dysfunction of the EVs to apolipoprotein E gene variant (APOE ε4) and the risk of progression to Alzheimer's disease (AD). To better understand the...
Article
Unhealthy dietary habits can play a crucial role in metabolic damages, promoting alteration of neural functions through the lifespan. Recently, dietary change has been perceived as the first line intervention in prevention and/or treatment of metabolic damages and related diseases. In this context, our study was designed to assess the eventual ther...
Article
Alzheimer disease (AD) and vascular dementia (VaD) are the most common form of dementia worldwide. Actually, the search for early peripheral biomarkers for AD and other dementia represent a great challenge. Exosomes or extracellular vesicles (EVs) are key players in cerebral function. We have demonstrated that the levels of some pathogenic proteins...
Article
Alzheimer’s disease (AD) is characterized by a series of overlapping pathophysiological cascades, including the aggregation of β‐amyloid plaques and the formation of neurofibrillary tangles derived from hyperphosphorylated tau proteins. Actually, the search for peripheral biomarkers for AD that reflect AD‐related processes in the brain has received...
Article
Advanced glycation end‐products (AGEs), their precursors methylglyoxal (MG), glyoxal (GO) and their receptors RAGEs are known to be involved in the pathophysiology of Alzheimer’s disease (AD). The aim of our study was to evidence the presence of RAGEs and glyoxalase‐1 (GLO‐1), the main enzyme involved in the degradation of MG, in the circulating ex...
Article
Treatments of neurodegenerative diseases (NDDs) are severely hampered by the presence of the blood-brain barrier (BBB) precluding efficient brain drug delivery. The development of drug nanocarriers aims at increasing the brain therapeutic index would represent a real progress in brain disease management. PEGylated polyester nanoparticles (NPs) are...
Article
The blood-brain barrier prevents passage of large and hydrophilic molecules, undermining efforts to deliver most active molecules, proteins and other macromolecules. To date, nanoparticle-assisted delivery has been extensively studied to overcome this challenge but with limited success. On the other hand, for certain brain therapeutic applications,...
Article
Nanoparticulate carriers, often referred to as nanoparticles (NPs), represent an important pharmacological advance for drug protection and tissue-specific drug delivery. Accessing the central nervous system (CNS), however, is a complex process regulated by mainly three brain barriers. While some leukocyte (i.e., immune cell) subsets are equipped wi...
Chapter
Fundamental causes of neurodegenerative diseases (NDDs) such as Alzheimer’s (AD) and Parkinson’s diseases (PD) remain a matter of debate, and the discovery of disease-modifying treatments represents a great challenge. Currently, patients with NDD have only access to actives intended to alleviate symptoms and not to target fundamental causes of the...
Article
Full-text available
Major phenolic compounds from olive oil (ArOH-EVOO), oleuropein (Ole), tyrosol (Tyr), and p-coumaric acid (p-Cou), are known for their antioxidant and neuroprotective properties. We previously demonstrated that their combination could potentiate their antioxidant activity in vitro and in cellulo. To further our knowledge of their electron-transfer...
Article
Background Diabetes is considered as a risk factor for Alzheimer’s disease, but it is yet un-clear whether this pathological link is reciprocal. Although Alzheimer’s disease and diabetes appear as entirely different pathological entities affecting the central nervous system and a peripheral organ (pan-creas), respectively, they share a common patho...
Article
Accumulating evidence suggests that the propagation of hyperphosphorylation of tau protein and the amyloid-β peptide can be mediated by extracellular vesicles (EVs) and be associated with the onset and the progression of Alzheimer's disease (AD). As EVs may transfer between the brain and the blood, we have thus hypothesized that the total plasma EV...
Article
Phenolic compounds from olive oil (ArOH-EVOO) are recognized for their antioxidant and neuroprotective capacities, but are often studied individually or through a natural extract. As their reactivity towards reactive oxygen species (ROS) depends on their structure and could implicate different complementary mechanisms, we hypothesized that their ef...
Article
Full-text available
Methylglyoxal (MG) and glyoxal (GO) are suggested to be associated with the development of neurodegenerative pathologies. However, their peripheral levels in relation to cognitive decline and their effects on key factors in neuronal cells are poorly investigated. The aim of this study was to determine their serum levels in MCI (mild cognitive impai...
Article
Full-text available
Background: Both advanced glycation end products (AGEs) N-(1-carboxymethyl)-L-lysine (CML) and pentosidine were found in the brain from Alzheimer's disease (AD) patients and were associated with the neuropathological hallmarks of AD. In AD patients, the circulating level of both AGEs remains unknown. Moreover, their levels in peripheral extracellu...
Article
Caloric restriction (CR) is the most effective intervention to improve health span and extend lifespan in preclinical models. This anti-aging effect of CR is related to attenuation of oxidative damage in various tissues, with divergent results in the brain. We addressed how brain oxidoreductive balance would be modulated in male Sprague-Dawley (SD)...
Article
Drug nanocarriers surface chemistry is often presumed to be uniform. For instance, the polymer surface coverage and distribution of ligands on nanoparticles are described with averaged values obtained from quantification techniques based on particle populations. However, these averaged values may conceal heterogeneities at different levels, either...
Article
Oxidative stress plays a pivotal and early role in the pathophysiology of Alzheimer's disease (AD). There is convincing evidence that oxidative alterations in AD and in mild cognitive impairment (MCI) patients are not limited to the brain but are extended to the blood compartment. However, the oxidative pattern in plasma is still inconclusive. More...
Presentation
La maladie d’Alzheimer (MA) touche environ 47 millions de personnes dans le monde. Par conséquent, une meilleure compréhension des mécanismes pathologiques et le développement d’un traitement efficace représente un des enjeux majeurs du XXIème siècle. La MA est associée à un déclin de la mémoire et des fonctions cognitives. Dans le cerveau, les apo...
Article
Full-text available
Canine cognitive dysfunction (CCD) is an age-dependent neurodegenerative condition characterised by changes in decline in learning and memory patterns. The neurodegenerative features of CCD in ageing dogs and cats are similar to human ageing and Alzheimer's disease (AD). Discovering neuroprotective disease-modifying therapies against CCD and AD is...
Article
An early diagnosis of Alzheimer's disease (AD) is required for early and more effective treatment. Oxidative stress plays an early role in AD and could promote the formation of advanced glycation end products (AGEs) in AD brains. However, the peripheral association of specific AGEs and oxidative markers with cognitive decline remains to be defined...
Chapter
Alzheimer’s disease (AD) is a progressive neurodegenerative disease with several preclinical and clinical stages. There is growing interest in investigating early biomarkers to adequately define the at-risk state which may ultimately allow preventive strategies and therapeutic agents for the disease- modifying status of AD. The validation of biomar...
Article
One major challenge in the field of nanotherapeutics is to increase the selective delivery of cargo to targeted cells. Using Poly Lactic-co-Glycolic Acid (PLGA), we recently highlighted the importance of polymer composition in the biological fate of the nanodrug delivery systems. However the route of internalisation of polymeric nanoparticles (NPs)...
Article
In Alzheimer's disease (AD) and in mild cognitive impairment (MCI) patients, by-products of lipid peroxidation such as acrolein accumulated in vulnerable regions of the brain. We have previously shown that acrolein is a highly reactive and neurotoxic aldehyde and its toxicity involves the alteration of several redox-sensitive pathways. Recently, pr...
Article
Curcumin, a neuroprotective agent with promising therapeutic approach has poor brain bioavailability. Herein, we demonstrate that curcumin-encapsulated PLGA 50:50 nanoparticles (NPS-Cur 50:50) is able to prevent the phosphorylation of Akt and Tau proteins in SK-N-SH cells induced by H2O2, displays higher anti-inflammatory and antioxidant activities...
Conference Paper
La maladie d'Alzheimer (MA) est la forme la plus fréquente des maladies neurodégénératives (MND). Selon l’Organisation Mondiale de la Santé, elle représenterait 50% des démences qui touchent 37 millions de personnes (von Bernhardi et al., 2012). La MA, de progression lente et irréversible, est caractérisée par une atrophie structurale, des altérati...
Poster
Protein Carbonyl, Total Antioxidant Capacity and Reduced Thiols in Plasma from Alzheimer’s Patients Is Inversely Associated with Cognitive Performance.
Conference Paper
Introduction: PLGA is a copolymer of D,L-Lactide (LA) and glycolic acid (GA) with a wide range of LA:GA ratio and molecular weights and therefore can display various physicochemical and degradation characteristics for controlled drug release applications according to the matrix used. PLGA polymer has been extensively studied for the development of...
Article
We developed a nanoparticles (NPs) library from poly(ethylene glycol)-poly lactic acid comb-like polymers with variable amount of PEG. Curcumin was encapsulated in the NPs with a view to develop a delivery platform to treat diseases involving oxidative stress affecting the CNS. We observed a sharp decrease in size between 15 and 20% w/w of PEG whic...
Article
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder of aging. It is a multifactorial disease with several overlapping pathways. Therefore, successful therapy should target several pathological features simultaneously. In this regard, cumulative data have demonstrated that polyphenols can display neuroprotective effects through...
Conference Paper
Actual challenge in the field of nanoneuropharmacology is converging to optimize the efficient uptake of active compounds into the CNS through polymer based nanoparticles. The relationships between the polymer composition and the biological fate are still unclear. This study aimed to provide the effect of polyethylene glycol (PEG) content and archi...
Conference Paper
Actual challenge in the field of nanoneuropharmacology is converging to optimize the efficient uptake of active compounds into the CNS through polymer based nanoparticles. The relationships between the polymer composition and the biological fate are still unclear. This study aimed to provide the effect of polyethylene glycol (PEG) content and archi...
Conference Paper
Werner syndrome (WS) is an autos omal recessive disorder associated with mutations in a gene encoding for a RecQ - type DNA helicase. This enzyme is involved in different aspects of DNA repair, replication, and transcription. Clinically, WS is one of the most intriguing disorders characteri zed by an accelerated aging phenotype. Interestingly, Alzh...
Article
Oxidative stress is involved in the etiology of multiple chronic diseases including Parkinson’s and Alzheimer’s diseases (AD). Moreover, it is believed to be a primary cause in diseases pathogenesis. For instance, in AD lipid peroxidation by-products such as acrolein accumulated specifically in vulnerable regions of the brain from mild cognitive im...
Article
Berberine (BBR), one of the major constituents of Chinese herb Rhizoma coptidis, has been reported to exert beneficial effects to various diseases, including Alzheimer's disease (AD). In the present work, we aimed to investigate the effects of BBR on neuronal cell death induced by homocysteic acid (HCA), which was considered as a risk of AD. BBR si...
Article
Aims: Oxidative stress (OS) plays an important role in the pathogenesis of neurodegenerative diseases, including Alzheimer's disease (AD). This study was designed to uncover the cellular and biochemical mechanisms underlying the neuroprotective effects of tacrine-3-caffeic acid (T3CA), a novel promising multifunctional anti-Alzheimer's dimer, agai...
Article
Acrolein is a highly electrophilic alpha, beta-unsaturated aldehyde to which humans are exposed in many situations and has been implicated in neurodegenerative diseases, such as Alzheimer's disease. Lithium is demonstrated to have neuroprotective and neurotrophic effects in brain ischemia, trauma, neurodegenerative disorders, and psychiatric disord...
Article
The aim of the present study was to examine the neuroprotective effect of curcumin against the toxicity induced by acrolein and to identify its cellular mechanisms and targets. Human neuroblastoma cells SK-N-SH were treated with acrolein. Curcumin, from 5 μM, was able to protect SK-N-SH cells against acrolein toxicity. The addition of curcumin rest...
Article
Previous studies have suggested that elevated blood Homocysteic acid (HCA) levels increased the risk of Alzheimer's disease (AD), but the underlying mechanisms are unclear. Herein, we studied the neuronal toxicity of HCA and the underlying mechanisms in HT-22 cells. Results showed that HCA induced cell death in concentration- and time-dependent man...
Article
Parkinson's disease (PD) is one of the most common age related neurodegenerative disease and affects millions of people worldwide. Strong evidence suggests a role for oxidative stress and mitochondrial dysfunctions in the pathogenesis of PD. Recent epidemiologic and toxicological studies have shown that environmental factors, especially herbicides...
Article
Acrolein-induced oxidative stress is hypothesized to involve in the etiology of Alzheimer's disease (AD). Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) have antioxidative and neuroprotective properties. The present study investigated the protective effects of CA/CAPE on acrolein-induced oxidative neuronal toxicity. HT22 mouse hippocampa...
Article
The pathologic mechanisms of Alzheimer's disease (AD) haven't been fully uncovered. Acrolein, a ubiquitous dietary pollutant and by-product of oxidative stress, can induce cytotoxicity in neurons, which might play an important role in the etiology of AD. Here, we examined the effects of Acrolein on the AD pathologies in vitro and in vivo. We found...
Article
Nanofiltration (NF) (pressure-driven process) and electrodialysis with ultrafiltration membrane (EDUF) (electrically-driven process) were compared in terms of mass flux and mass balance. Molecular weight profile and protein, peptide and amino acids contents of the fractions obtained were also analysed. The two processes lead to different results si...
Article
The aims of this work were 1) to determine the bioactivities resulting from the hydrolysis of soy (Glycine max) proteins isolate (SPI) by a double enzymatic treatment of pepsin and pancreatin and 2) to investigate whether fractionation of the protein hydrolysates by two ultrafiltration techniques using either hollow fiber (HF) or spiral-wounded (SW...
Article
Curcumin, a natural polyphenolic pigment present in the spice turmeric (Curcuma longa), is known to possess a pleiotropic activity such as antioxidant, anti-inflammatory, and anti-amyloid-β activities. However, these benefits of curcumin are limited by its poor aqueous solubility and oral bioavailability. In the present study, a polymer-based nanop...
Article
Parkinson's disease (PD) is one of the most common age-related neurodegenerative diseases and affects millions of people worldwide. Strong evidence supports the role of free radicals, oxidative stress, mitochondrial, and proteasomal dysfunctions underlying neuronal death in PD. Environmental factors, especially pesticides, represent one of the prim...
Article
Alzheimer's disease (AD) is a multifactorial disorder characterized by the presence of amyloid plaques and neurofibrillary tangles (NFTs). Rare early-onset forms of AD are associated with autosomal dominant mutations in the amyloid precursor protein gene, presenilin 1 gene, or presenilin 2 gene. The late-onset form of the disease (LOAD) is the most...
Article
Curcumin, the phytochemical agent in the spice turmeric, which gives Indian curry its yellow colour, is also a traditional Indian medicine. It has been used for millennia as a wound-healing agent and for treating a variety of ailments. The antioxidant, anti-inflammatory, antiproliferative and other properties of curcumin have only recently gained t...
Article
It is estimated that more than 24 million people sufferhave from dementia today and Alzheimer's disease (AD) represents the most common form of dementia. Therefore, there is a critical need to develop new strategies to prevent or to slow down the progression of AD. Recent studies suggested that certain diets, beverages, polyphenols, functional food...
Chapter
In developing countries, ageing of the population leads to an increase of cases with cognitive impairment, the most dramatic form being dementia. Dementia is characterised by the alteration of superior cognitive functions such as memory, attention or executive functions. The most prevalent cause of dementia is Alzheimer's disease (AD). At present,...