Charles L Guy

University of Florida | UF · Department of Environmental Horticulture

Background: There is mounting anecdotal and empirical evidence that gardening and art-making afford therapeutic benefits. Objectives: This randomly controlled pilot study tested the hypothesis that participation in group-based indoor gardening or art-making activities for one hour twice a week for four weeks would provide quantifiably different...

Additional index words. therapeutic horticulture, arts in medicine, mental health A human subject experiment involving healthy women ages 26-49 was conducted where study participants were randomly assigned to either a gardening or art intervention consisting of eight one-hour sessions over a four-week period. Self-report psychometric assessments w...

Plants provide people with vital resources necessary to sustain life. Nutrition, vitamins, calories, oxygen, fuel, and medicinal phytochemicals are just a few of the life-supporting plant products, but does our relationship with plants transcend these physical and biochemical products? This review synthesizes some of the extant literature on people...

Petrifilms are dehydrated agar culture plates that have been used to quantify colony forming units (CFU) mL of either aerobic bacteria (Petrifilm-AC) or fungus (Petrifilm-YM), depending on substrate composition. Microbes in irrigation systems can indicate biofilm risk and potential clogging of irrigation emitters. The research objective was to comp...

Thesis (M.S.)--Florida Technological University. Includes bibliographical references (leaves 68-74).

Arsenate interferes with enzymatic processes and inhibits phosphate uptake in plants. This study examined the role of phytase and phosphatase in arsenate tolerance and P acquisition in the arsenic hyperaccumulator Pteris vittata. Enzyme-mediated hydrolysis of phytate in P. vittata extracts was not inhibited by arsenate at 5 mM or by heating at 100°...

The objective was to analyze the physical, chemical, and biological water quality in horticulture irrigation systems in 24 ornamental plant greenhouses and nurseries in the United States. At each greenhouse or nursery, water was collected from up to five points ("Sample Types") which included 1) "Source" from municipal or private well supplies, 2)...

Many plants of tropical and subtropical origin are severely damaged when exposed to chilling temperatures between 2 and 15°C. In contrast, the cruciferous plant Arabidopsis thaliana is chilling tolerant and, therefore provides an alternative model plant system for the identification of chilling tolerance traits. In this chapter, we describe physiol...

In tightly closed human habitats such as space stations, locations near volcano vents and closed culture vessels, atmospheric CO(2) concentration may be 10 to 20 times greater than Earth's current ambient levels. It is known that super-elevated (SE) CO(2) (>1,200 µmol mol(-1)) induces physiological responses different from that of moderately elevat...

CO2 or developmental responsive low molecular weight compounds with 2 fold or more changes in their steady state levels in mixed effect model analysis (p-value ≤0.001). (XLSX)

A. Corresponding genes of the development-responsive transcripts with 2 fold or more changes in their signals in mixed effect model analysis (p-value ≤0.001). B. Corresponding genes of the CO2 regulated transcripts. CO2 only or development independent and dependent CO2 regulated transcripts with 2 fold or more changes in their signals in mixed effe...

The raw data for metabolite profiling. n = 3 biological experiments each having 3 analytical replicates (XLSX)

CO2 responsive transcripts with biotic abiotic, hormone, and sugar regulation. The transcripts in Table S2 are compared to publicly available microarray data sets. (XLSX)

The raw data for transcript profiling. n = 3 biological experiments. (XLSX)

In tightly closed human habitats such as space stations, locations near volcano vents and closed culture vessels, atmospheric CO2 concentration may be 10 to 20 times greater than Earth’s current ambient levels. It is known that super-elevated (SE) CO2 (>1,200 µmol mol−1) induces physiological responses different from that of moderately elevated CO2...

In tightly closed human habitats such as space stations, locations near volcano vents and closed culture vessels, atmospheric CO2 concentration may be 10 to 20 times greater than Earth’s current ambient levels. It is known that super-elevated (SE) CO2 (>1,200 µmol mol−1) induces physiological responses different from that of moderately elevated CO2...

DEAD-box RNA helicases compose a large subfamily of RNA helicases found in all eukaryotes and prokaryotes. Functional DEAD-box RNA helicases are considered to be RNA chaperones that modify RNA secondary structure and perhaps three-dimensional structures during different cellular processes involving RNA metabolism. Although a relationship of DEAD-bo...

Schematic gene diagram of AtRH22 and AtRH52. Exons and introns are represented by boxes and lines, respectively. Insertion positions of T-DNA insertion are marked with arrowheads. Primers used to determine the genotype and orientation of the T-DNA insertion are displayed

List of primers used in this study

The objective was to analyze physical, chemical and biological water quality in horticulture irrigation systems, from a survey of 24 ornamental plant nurseries in the U.S. (“Locations”). Within each Location, water was collected from up to five points (“Sample Types”) which included “Source” from municipal or well supplies, “Tanks” from enclosed st...

Grapefruit (Citrus × paradisi) develop symptoms of chilling injury (CI) if held at temperatures below about 10 °C. Conditioning grapefruit at a low, but non-chilling (16 °C) temperature prior to storage at a chilling temperature reduces the development of CI symptoms. Changes in transcript abundance for a number of genes have been correlated with c...

The formation of ice on and inside plant tissues represents a major challenge to survival. The resulting phase transition and spatial redistribution of liquid water from inside the cell to extracellular ice results in physical changes to cells and enormous physical stresses and strains. The ability of higher plants to acclimate and tolerate freezin...

The fully annotated Figure 7: hierarchical average linkage cluster analysis of transporter gene expression using uncentered correlation. Hierarchical average linkage cluster analysis of transporter gene expression using uncentered correlation. The cluster analysis is based on transporter genes with significant expression at Time 45, 90 or 180. Yell...

Plant growth performance ANOVA. ANOVA (t-test) results are shown for specific genotype concentration effects in the growth experiments. (0.01 MB XLS)

Root transcriptome ANOVA. An ANOVA analysis of differential expression in the root transcriptome. (0.71 MB XLS)

Martian regolith (unconsolidated surface material) is a potential medium for plant growth in bioregenerative life support systems during manned missions on Mars. However, hydrated magnesium sulfate mineral levels in the regolith of Mars can reach as high as 10 wt%, and would be expected to be highly inhibitory to plant growth. Disabling ion transpo...

Leaf senescence is a programmed developmental process governed by various endogenous and exogenous factors, such as the plant developmental stage, leaf age, phytohormone levels, darkness, and exposure to stresses. It was found that, in addition to its well-documented role in the enhancement of plant frost tolerance, overexpression of the C-repeat/d...

Plants possess inducible tolerance mechanisms to temperature extremes that contribute to survival, yet many aspects of stress-inducible responses remain poorly understood. One example is the cold induction of pyruvate decarboxylase and alcohol dehydrogenase gene expression that has long been a mystery. In the present work, comparative transcriptome...

Analysis of three plastid DNA sequences for a broad sampling of Amaryllidaceae resolve the American genera of the Amaryllidaceae as a clade that is sister to the Eurasian genera of the family, but base substitution rates for these genes are too low to resolve much of the intergeneric relationships within the American clade. We obtained ITS rDNA seq...

The metabolic consequence of suboptimal (400 μmol mol−1 or ppm), near-optimal (1500 ppm) and supra-optimal (10,000 ppm) atmospheric carbon dioxide concentrations [CO2] was investigated in an attempt to reveal plausible underlying mechanisms for the differential physiological and developmental responses to increasing [CO2]. Both non-targeted and tar...

Plants possess inducible tolerance mechanisms that extend the temperature range for survival during acute temperature stress. The inducible mechanisms of cold acclimation and acquired thermotolerance involve highly complex processes. These include perception and signal transduction of non-optimal temperatures or their physical consequences on cellu...

A pre-storage conditioning (CD) treatment of 16 degrees C for 7 d enhanced chilling tolerance of grapefruit and reduced the development of chilling injuries during storage at 5 degrees C. To gain a better understanding of the molecular mechanisms involved in the responses of citrus fruit to low temperatures, we performed genome-wide transcriptional...

Glucan phosphorylating enzymes are required for normal mobilization of starch in leaves of Arabidopsis (Arabidopsis thaliana) and potato (Solarium tuberosum), but mechanisms underlying this dependency are unknown. Using two different activity assays, we aimed to identify starch degrading enzymes from Arabidopsis, whose activity is affected by gluca...

Exposure of Arabidopsis to low temperatures results in cold acclimation where freezing tolerance is enhanced. To achieve a wider view of the role of transcriptome to biochemical changes that occur during cold acclimation, analyses of concurrent transcript and metabolite changes during cold acclimation was performed revealing the dynamics of selecte...

Hsp70 molecular chaperones have been shown to play an important role in helping cells to cope with adverse environments, especially in response to high temperatures. The molecular chaperone function of Hsc70 at low temperature was investigated. A cold-inducible spinach cytosolic Hsc70 was subcloned into a protein expression vector and the recombina...

Cold-acclimation-induced changes in freezing tolerance and translatable RNA content were compared in seedlings of a relatively cold sensitive citrus species, Citrus grandis L. Osb. cv. Thong Dee (pummelo), and the cold-hardy citrus relative, Poncirus trifoliata L. Raf. cv. Pomeroy (trifoliate orange). Cold acclimation of pummelo (10 days at 15°C fo...

Abstract Cold-acclimated stems of red-osier dogwood (Cornus sericea L.) were sampled in midwinter and early spring and subjected to the following low temperature treatments: (a)0 →−40 → 0°C; (b) 0 →−40 →− 196 → 0°C; (c) 0 →−40 →−196 →−269 →−196 → 0°C; (d) 0 →−40 →−269 →−196 → 0°C; (e) 0 →−196 → 0°C; (f) 0 →−269 →−196 →0°C. The cortical parenchyma c...

Abstract Seasonal cold-acclimation patterns and the effects of photoperiod and temperature on cold-hardiness of Hibiscus rosa-sinensis L. and Hibiscus syriacus L. were determined. Field-grown H. rosasinensis consistently failed to survive freezing at - 2°C. Two genotypes of field- and container-grown H. syriacus initiated cold-acclimation in mid Se...

In 1975 O'Farrell described, in detail, a procedure to separate proteins by polyacrylamide gel electrophoresis in two dimensions. This powerful new technique relied on two characteristics of proteins: charge and molecular mass. In the first dimension, proteins were separated on the basis of net charge in a pH gradient by isoelectric focusing, and i...

A combination of hot water (a rinse at 62 degrees C for 20 s) and conditioning (pre-storage at 16 degrees C for 7 d) treatments synergistically reduced chilling injury development in grapefruit (Citrus paradisi, cv. "Star Ruby") during cold storage at 2 degrees C, suggesting that the treatments may activate different chilling tolerance responses. T...

A primary role of β-amylase (BMY) is to produce maltose during hydrolytic starch degradation. In photosynthetic organs, BMY activity is present in the chloroplast, where substrate starch is localized. BMY activity is also found in the vacuole and cytosol, where starch is not known to be localized suggesting additional roles in glucan degradation. T...

It has been suggested that beta-amylase (BMY) induction during temperature stress in Arabidopsis could lead to starch-dependent maltose accumulation, and that maltose may contribute to protection of the electron transport chain and proteins in the chloroplast stroma during acute stress. A time-course transcript profiling analysis for cold shock at...

The exposure of Arabidopsis thaliana (Arabidopsis) plants to spaceflight environments results in differential gene expression. A 5-day mission on orbiter Columbia in 1999 (STS-93) carried transgenic Arabidopsis plants engineered with a transgene composed of the alcohol dehydrogenase (Adh) gene promoter linked to the β-Glucuronidase (GUS) reporter g...

Visualizing and analysing the potential non-linear structure of a dataset is becoming an important task in molecular biology. This is even more challenging when the data have missing values. Here, we propose an inverse model that performs non-linear principal component analysis (NLPCA) from incomplete datasets. Missing values are ignored while opti...

In animals and yeast, cytosolic Hsp70s function in concert with other molecular chaperones. Hsp70 is a major chaperone in the Hsp90 multi-chaperone complexes that participate in maturation of steroid receptors and several other proteins. Hsp70s also appear to form a complex with Hsp90 and Hsp110/sHsp. A 100 kDa protein was co-immunoprecipitated wit...

Regulatory control in biological systems is exerted at all levels within the central dogma of biology: DNA→messenger RNA (mRNA)→Enzymeinactive→ Enzymeactive→Metabolites (Figure 1). Metabolites are the end products of all cellular regulatory processes and reflect the ultimate outcome of potential changes suggested by genomics and proteomics caused b...

Metabolic profiling analyses were performed to determine metabolite temporal dynamics associated with the induction of acquired thermotolerance in response to heat shock and acquired freezing tolerance in response to cold shock. Low-M(r) polar metabolite analyses were performed using gas chromatography-mass spectrometry. Eighty-one identified metab...

A number of studies have demonstrated beta-amylase induction in response to abiotic stress. In the present work, a temperature response profile in 5 degrees C increments from 45 degrees C to 0 degrees C showed that induction at temperature extremes was specific for two members of the gene family (BMY7 and BMY8). Both members encode proteins that po...

Hsp70s function as molecular chaperones. The protective chaperone activities of hsp70 help to confer tolerance to heat, glucose deprivation, and drought. Overexpression of hsp70s in many organisms correlates with enhanced thermotolerance, altered growth, and development. To better understand the roles of hsp70 proteins in Arabidopsis, the molecular...

Acquired tolerance to temperature stresses is a major protective mechanism. Recent advances have revealed key components of stress signal transduction pathways that trigger enhanced tolerance, and several determinants of acquired tolerance have been identified. Although high and low temperature stresses impose different metabolic and physical chall...

The generic limits of Hymenocallis have been variously proposed by different taxonomic workers, often without discussion or data. The genera Leptochiton, Ismene, Elisena, and Pseudostenomesson have been included with Hymenocallis, lumped together as the genus Ismene, or maintained as distinct genera. Recent cladistic analysis of plastid and nrDNA f...

Salt stress responses of C. grandis (L.) Osb., P. trifoliata (L.) Raf. and their F1 (17-40) were investigated. Different growth parameters, including shoot elongation, leaf, stem, structural root (> 2mm diameter), fine root (< 2mm diameter) and whole plant dry mass production as well as different tissue and whole plant accumulations of 11 macro and...

Poncirus trifoliata (L.) Raf. and Citrus grandis (L.) Osb. were phenotypically distinct and their selected F1, 17-40, had features that displayed a combination of both parents. Favorable alleles at genes that condition traits for high leaf mass proportion, abundant fibrous root production, and superior tissue tolerance to high Na concentration in f...

The effects of salinization with 40 mM sodium chloride on P. trifoliata (L.) Raf., C. grandis (L.) Osb. and their F1, and a BC1 progeny population [C. grandis x (C. grandis x P. trifoliata)] were investigated. A total of 272 traits were analyzed in salinized and non-salinized BC1 progeny, including parameters of growth, tissue and whole plant dry m...

Barley (Hordeum vulgare L.) exposed to low temperature increases its freezing tolerance. This increase has been associated with several metabolic changes caused by low temperature, including expression of dehydrins (DHN), a family of proteins induced by dehydration and cold acclimation. DHNs play an undetermined role in dehydration responses during...

Temperature stress is an assured reality for all horticultural crops, as nearly every plant experiences some form of temperature stress during its life cycle. To cope with temperature extremes beyond the range of an organism's optimum for growth, stress responses and survival strategies have evolved to varying degrees in an organism-specific fashio...

The Hsp70 molecular chaperones of plants are encoded by a multi-gene family whose members are developmentally regulated and differentially expressed in response to temperature stress and other conditions that interrupt normal protein folding or favor protein denaturation. Under non-stressful conditions, Hsp70 cognates function in concert with a var...

We isolated cDNA clones for two nuclear-encoded, organellar members of the Arabidopsis hsp70 gene family, mtHsc70-2 (AF217458) and cpHsc70-2 (AF217459). Together with the completion of the genome sequence, the hsp70 family in Arabidopsis consists of 14 members unequally distributed among the five chromosomes. To establish detailed expression data o...

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) va...

Expression of six Hsp70s in spinach (Spinacia oleracea cv Longstanding Bloomsdale) leaves grown under isothermal conditions is regulated by a light/dark (L/D) mechanism distinctly different from the light-regulated mechanism for the chlorophyll a/b-binding protein (cab) or small subunit of ribulose-1,5-bisphosphate carboxylase oxygenase (rbcS). Sub...

The standard amplified fragment-length polymorphism (AFLP) technique was modified to develop a convenient and reliable technique for rapid genetic characterization of plants. Modifications included (i) using one restriction enzyme, one adapter molecule and primer, (ii) incorporating formamide to generate more intense and uniform bands and (iii) usi...

Quantitative trait loci (QTLs) influenced by salinization have been identified in a previously mapped BC1 progeny population of (C. grandis) x (C grandis x P. trifoliata). Each progeny plant was vegetatively propagated to provide multiple control and salinized clones. Traits assayed included growth and dry weight, and Na+ and Cl- accumulation in in...

Steady-state mRNA levels for three Hsp70s were found to be regulated by a distinctive light/dark mechanism in spinach leaves. Messenger RNAs for the chloroplast stromal and two cytosolic forms displayed a diurnal expression pattern under isothermal conditions that appeared to be independent of circadian control. While protein blot data showed relat...

Salt stress responses of C. grandis L. (Osb.), P. trifoliata (L.) Raf. and their F 1 were investi-gated. Growth, growth rates, as well as leaf, stem, structural root (> 2 mm diameter), fine root (≤ 2mm diameter) and whole plant dry masses were determined for the three genotypes tested in 0, 40 and 80 mМ NaCl environments for 20 weeks. P. trifoliata...

The effects of salinization with 0, 30, 60, 90, and 120 mМ sodium chloride (NaCl) on Poncirus trifoliata (L.) Raf. cv. Pomeroy were studied by means of stem elongation patterns, whole plant and tissue mass production, and mineral nutrient accumulation. The elements analyzed in leaf, stem, structural root and fine root tissues included Na, Cl, P, K,...

The Plant Kingdom encompasses a grouping of mostly sessile organisms that show extreme variation in morphology, size, ecological adaptation, life cycle, and climatic tolerance. With the exception of low elevation tropical environments, plants living just about anywhere else in the world may be subject to temperatures below that which are optimal fo...

Cladistic analyses of plastid DNA sequences rbcL and trnL-F are presented separately and combined for 48 genera of Amaryllidaceae and 29 genera of related asparagalean families. The combined analysis is the most highly resolved of the three and provides good support for the monophyly of Amaryllidaceae and indicates Agapanthaceae as its sister famil...

The effects of salinization with 40 mM NaCl on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F1, and a BC1 progeny population (C. grandis × (F1)) were investigated by means of Na+ and Cl- analyses and QTL (quantitative trait loci) mapping. A total of 38 traits related to different tissue or whole-plant Na+ and (or) Cl- accumulation...

The effects of salinization with 40 mM sodium chloride on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F1, and a BC1 progeny population [C. grandis × (F1)] were investigated by measuring growth and dry mass production of different tissues and by QTL (quantitative trait locus) mapping. A total of 36 traits related to growth (six tr...

Stress 70 molecular chaperones are found in all the major subcellular compartments of plant cells, and they are encoded by a multigene family. Twelve members of this family have been identified in spinach. The expression of the stress 70 molecular chaperones in response to heat shock is well-known and it appears that low temperature exposure can al...

Molecular chaperones of the stress 70 family reversibly bind and release nonnative proteins in a nucleotide-dependent cycle. Purified monoclonal antibodies prepared against spinach (Spinacia oleracea) stress 70 molecular chaperones were used in immunoprecipitation experiments with extracts of spinach leaf tissue pulse-labeled with [35S]methionine i...

The cDNA sequence for CAP160, an acidic protein previously linked with cold acclimation in spinach (Spinacia oleracea L.), was characterized and found to encode a novel acidic protein of 780 amino acids having very limited homology to a pair of Arabidopsis thaliana stress-regulated proteins, rd29A and rd29B. The lack of similarity in the structural...

The stress 70 molecular chaperones of plants are localized and function in all of the major subcellular compartments of the cell. Collectively, all of the various forms are encoded by a multigene family in the nucleus. At least 12 members of this family have been found, and sequence and DNA blot analyses provide an emerging description of the diver...

On Dec 18, 1997 this sequence version replaced gi:2654211.