Charles S Brennan

RMIT University | RMIT · School of Science

The much publicised global trend in rising levels of obesity and diabetes has refuelled interest in the dietary intake of the macronutrients (fat, protein, and carbohydrates) necessary to maintain the state of normalcy (good health) of an individual. Both scientific and public attention have focused on the dietary mediation of chronic health syndro...

There is inconsistency between current in vitro digestion methods with regard to accommodation of a (salivary) α-amylase exposure during the oral phase. The effect of a salivary α-amylase pre-exposure on subsequent in vitro starch digestion curve profiles for various foods was investigated. Foods were chewed, expectorated and the boluses left to re...

A novel strain of Lactobacillus plantarum DMDL 9010 (CGMCC No. 5172) was isolated from naturally fermented mustard. The potential cholesterol reduction effects of this strain were investigated using an in vivo model. The results showed that L. plantarum DMDL 9010 at a dose of 109 cells per day significantly reduced (P ≤ 0.05) serum total cholestero...

The application of sugar replacers used in bakery products is of growing interest to the food industry, as it provides the possibility of delivering products with reduced energy and sugar. The aim of this study was to investigate the textural properties and glycaemic responses of muffins made using stevianna and inulin. Two levels of sugar replacer...

In this study, a partial substitution of durum wheat semolina with three different species of mushrooms (white button, shitake and porcini) was undertaken to increase the nutritional value of the pasta. The cooking properties and textural characteristics of the pasta produced were also determined. The results showed that the addition of mushroom po...

Development of Gluten-Free Pasta summarizes current progress in the development of gluten-free (GF) products, focusing particularly on pasta products. Presented in 11 chapters, the book focuses on the role of prebiotic fiber, hydrocolloids, fruit and vegetable by-product pomace and the physical, microstructural, sensory, and nutritional properties...

A high glycemic index diet can lead to elevated blood glucose levels and chronic diseases. In this study, Dictyophora indusiata powder, oats powder, and Polygonatum kingianum powder were substituted for some wheat flour to make pizza crust. The aim was to investigate the effects of the three powders on the chemical composition, physical quality, an...

Bacillus cereus is an important zoonotic foodborne conditional pathogen. It is found in vegetables, dairy products, rice, and other foods, thereby greatly endangering human health. Investigations on B. cereus contamination in China primarily focus on raw milk, dairy products, meat, and others, and limited research has been conducted on plant-based...

The aim of this study was to investigate the ability of shell (coating) formulations comprised of alginate and glucono delta lactone (GDL) to encapsulate a mixture of green tea and turmeric extracts. Three concentrations of alginate and GDL were used at 0.5%, 0.75%, and 1%, w/v and their solid ratio was varied using a factorial design. A response s...

The aim of this study was to prepare a novel pH-sensitive smart film based on the addition of purple garlic peel extract (PGE) and TiO2 nanoparticles in a sodium alginate (SA)/polyvinyl alcohol (PVA) matrix to monitor the freshness of beef. FT-IR spectroscopy revealed the formation of stronger interaction forces between PVA/SA, PGE, and TiO2 nanopa...

Due to the global rise in food insecurity, micronutrient deficiency, and diet-related health issues, the United Nations (UN) has called for action to eradicate hunger and malnutrition. Grains are the staple food worldwide; hence, improving their nutritional quality can certainly be an appropriate approach to mitigate malnutrition. This review artic...

Low-pressure plasma (LPP) treatment was implemented as a nonthermal technology to preserve the organoleptic and nutritional qualities of lyophilized lemon slices. Effects of LPP treatment on the basic quality and flavor characteristics of lyophilized lemon slices stored for 7 d were evaluated. Fresh lyophilized lemon slices were prepared as a refer...

Medium‐chain fatty acids (MCFAs) are naturally associated with human milk lipids and can also be associated with MCTs (medium‐chain triacylglycerols). Healthy lipids could be constructed from medium‐ and long‐chain triacylglycerols (MLCTs) composed of MCFAs. Biochemical and chemical catalysts catalyze many chemical and enzymatic processes, such as...

The effects of dairy ingredient substitution including whey protein concentrate (WPC), whey lactalbumin concentrate (WLAC) and skim milk powder (SMP) on functional properties of oat flour were investigated. Pasting analysis revealed that adding dairy ingredients at low substitution concentrations (5% and 10%) weaken the gel strength. WPC and WLAC r...

The aim of this research is to characterise the physicochemical and techno‐functional properties of the Moringa oleifera flower powder, and various techniques were used in this regard, such as FTIR, GC–MS, TGA, FESEM, and EDS. The bulk and tapped density of the Moringa oleifera flower powder were found to be 0.44 ± 0.03 (g/cm ³ ) and 0.51 ± 0.01 (g...

The effect of cassava-banana flour formulation and soy protein isolate (SPI) addition on gluten-free bread quality and antioxidant properties has been studied. The gluten-free flour composition was 25:75, 50:50, and 75:25 for cassava & banana flour with SPI levels ranged 0, 2.5, 5.0, and 7.5%. Wheat bread was used as control. Different gluten-free...

Objectives Obesity is the accumulation of excessive visceral and subcutaneous fat in the body. The herbal formulation can help treat obesity and our study aims to evaluate the acute basic biological effects of herbal formulation to control obesity. Methods Antiobesity herbal formulation, prepared by the combination of Saussurea lappa root, Betula...

Heterocyclic aromatic amines (HAAs) are detrimental substances can develop during the high-temperature cooking of protein-rich foods, such as meat. They are potent mutagens and carcinogens linked to an increased risk of various cancers. HAAs have complex structures with nitrogen-containing aromatic rings and are formed through chemical reactions be...

The aim of this study was to investigate the ability of shell (coating) formulations comprised of alginate and glucono delta lactone (GDL) to encapsulate a mixture of green tea and turmeric extracts. Three concentrations (0.5%, 0.75%, and 1%, w/v) of alginate and GDL were used, and their solid ratio was varied using a factorial design. A response s...

Shiitake polysaccharides acted as a non-competitive inhibitor to α- glucosidase and inhibited glucose transport of digested starch from Caco- 2 cells monolayer

The inhibition mechanism of shitake mushroom polysaccharides (Lentinula edodes polysaccharides, LEP) against α-glucosidase was studied by enzyme kinetic assay, fluorescence quenching and molecular docking. The effect of LEP on glucose transport of digested starch was investigated via an in vitro digestion/Caco-2 transwell model. LEP exhibited a str...

This work aimed to determine the effect of sealing process at different internal temperatures on the cooking loss, lipid oxidation, textural, and sensory properties, as well as the protein profile of cooked meat. For this aim, four different groups were formed. The control group was not sealed, while the other three groups were sealed until their i...

Quinoa is a pseudo-cereal with great nutritional and functional qualities, serving as an excellent substitution to develop quinoa-containing foods. This study aimed to explore the influence of quinoa flour substitution on quality characteristics of wheat flour (WF). WF was substituted with different level of quinoa core flour, ground quinoa whole f...

The present study investigates the effects of α-amylase (6 and 10 ppm), xylanase (70 and 120 ppm), and cellulase (35 and 60 ppm) on the physicochemical characteristics and nutritional quality of Chinese steamed bun (CSB) incorporated with 15% wheat bran (WB). Compared to the single enzyme, the combined enzymes improved the specific volume of CSB up...

Microbiota plays an essential role in human development and body homeostasis. Individual and environmental variables influence the diversity of microbiota, which performs crucial biochemical activities in the human body and influences health status and disease in later years. Many diseases in adulthood may be prevented or treated if the relationshi...

This study investigated the ability of acidic polysaccharides from Auricularia auricula-judae (AAP) and Tremella fuciformis (TFP) mushrooms to modulate starch digestion and absorption. Gelatinised sorghum starch was used as starch-rich material, and its digestion and glucose transport were determined through in vitro digestion/Caco-2 cells model. R...

The quality of fresh-cut Ma bamboo (Dendrocalamus latiflorus Munro) shoots was evaluated via physicochemical, 16S rRNA gene sequencing, and metabolomic analyses. Lactobacillaceae, Streptococcaceae, and Enterobacteriaceae were the predominant bacteria. Melatonin and UV-C treatments most inhibited Lactobacillaceae and Cyano-bacteriales, respectively....

The poor water solubility and rhein (RH) stability limit its application in the functional food industry. In the present study, the RH-loaded water-in-oil-in-water nano emulsion and microcapsules were prepared using the conjugates of pullulan-Jiuzao glutelin (JG) (m/m, 2:1, PJC-2) obtained by Maillard reaction and enteric-soluble materials (poly...

Meeting the United Nation’s sustainable development goals for zero hunger becomes increasingly challenging with respect to climate change and political and economic challenges. An effective strategy to alleviate hunger and its severe implications is to produce affordable, nutrient-dense, and sustainable food products. Ancient grains were long-forgo...

Phenolic compounds can form complexes with starch during food processing, which can modulate the release of phenolic compounds in the gastrointestinal tract and regulate the bioaccessibility of phenolic compounds. The starch-phenolic complexation is determined by the structure of starch, phenolic compounds, and the food processing conditions. In th...

Defatted peanut meal protein hydrolysates (DPMHs) usually have a bitter taste. γ-Glutamylation by Bacillus amyloliquefaciens l-glutaminase was introduced to DPMH to reduce its bitterness and generated a γ-glutamylated product (DPMH-G). Extra l-glutamine (l-Gln) (5% w/w) was added to DPMH, and the mixture was then γ-glutamylated (DPMH-G-Q). Results...

Abstract This study aimed at gaining insight into the mechanism of interactions between sorghum starch and mushroom polysaccharides (MPs) obtained from three cultivars Lentinula edodes (LEP), Auricularia auricula (AAP), and Tremella fuciformis polysaccharides (TFP) in relation to gelatinization, retrogradation and digestibility of starch, and micro...

Buckwheat is a good source of dietary fibre, protein and minerals, which has many beneficial effects on human health. However, previous research has illustrated that the addition of buckwheat has detrimental effects on the dough rheology and final products quality. Thus, the objective of this study was to investigate the effects of α-amylase (6 and...

A significant percentage of the world population suffers from non-communicable diseases (NCDs) such as diabetes, cardiovascular diseases, cancers, and obesity due to unhealthy food habits. There is an association between the ingestion of carbohydrate-dense food products and diabetes and obesity. Resistant (RS) starch is chemically tolerable to the...

A significant percentage of the world population suffers from noncommunicable diseases (NCDs) such as diabetes, cardiovascular diseases, cancers, and obesity due to unhealthy food habits. There is an association between the ingestion of carbohydrate-dense food products and diabetes and obesity. Resistant (RS) starch is chemically tolerable to the d...

Herein, some quality characteristics (water content, pH, antioxidant activity and sugar contents) of fresh garlic (Taşköprü, Türkiye) and black garlic, which were produced under various ageing temperatures (60 °C, 70 °C and 80 °C) and durations (30, 45 and 60 days), and the effect of ageing processes on these quality characteristics were determined...

Ganoderma lucidum is a medicinal mushroom that has been traditionally used in Chinese medicine for centuries. It has been found to have a wide range of medicinal properties, including antioxidant, anti-inflammatory, and immune-boosting effects. Recent research has focused on the potential benefits of G. lucidum in treating metabolic disorders such...

The aim of this study was to investigate the effects of extruded multigrain (Tartary buckwheat, oat and black bean) powder product (MG) fed with a high-fat-diet (HFD) on metabolism and gut microbiota modulation of mice. Thirty C57BL/6J mice were fed a normal diet (n = 10), HFD (n = 10) or HFD replacing 40% MG (HFMG, n = 10) for six weeks. The resul...

A better understanding of dough rheology during processing is crucial in the bakery industry, since quality attributes of the final product are influenced by those properties. In this study, we investigated the effects of xylanase, α-amylase and cellulase on the rheological properties of bread dough enriched in oat bran. A DoughLAB was used to meas...

The use of 3D printing (3DP) for food applications is gaining attention both as a research topic, and for industrial applications. Chocolate is one of the most common food commodities used for 3DP, owing to its melt extrusion capability and popularity in the high‐end food industry. However, chocolate remains a difficult substrate to work with, due...

The impact of using chia seeds at different rates (0%, 0.5%, 1%, and 1.5%) in meatball on the water content, pH and lipid oxidation (thiobarbituric acid reactive substances, TBARS), cooking loss and heterocyclic aromatic amines (HAAs) content of meatballs cooked at 150, 200, and 250 °C was investigated. The chia seed significantly affected water co...

Soy protein isolate and egg white protein were added to cassava-banana gluten-free pasta and the effects on the nutritional quality, digestibility properties, protein digestibility corrected amino acid (PDCAA), and sensory acceptance of the pasta was observed. Banana-cassava composite flour (75:25) was blended with soy protein isolate or egg white...

In this work, thermomechanically micronized sugar beet pulp (MSBP), a micron-scaled plant-based byproduct comprised of soluble elements (∼40 wt%) and insoluble fibrous particles (IFPs, ∼60 wt%), was used as a sole stabilizer for oil-in-water emulsion fabrication. The influence of emulsification parameters on the emulsifying properties of MSBP was i...

The effects of dietary fibre on gut microbiota and on the colonic markers of health have been well investigated, along with kinetics of fibre fermentation. Most of the evidence available is based on faecal analysis or static in‐vitro models of colonic fermentation of constant amounts of fibres incubated individually with faecal inoculum. These resu...

Gold nanoparticles (AuNPs) have been applied in a variety of fields based on their unique physical and chemical properties. Phenolic compounds (PCs) are the largest variety of phytochemicals in foods and have received substantial attention owing to their extraordinary health benefits. Recently, extensive studies have been conducted on the synthesis...

This study aimed to explore the effect of aged pork fat on the quality and volatile compounds of Chi-aroma Baijiu during the ageing stage. Result showed that glycerol and total ester contents of CBN, CB1, CB3 and CB5 were significantly higher than that in BL (P < 0.05), but total acid contents did not change significantly (P > 0.05). Moreover, ther...

The present study investigates the individual and interactional effects of α-amylase (6 and 10 ppm), xylanase (70 and 120 ppm) and cellulase (35 and 60 ppm) on the physicochemical characteristics and nutritional quality of Chinese steamed bread (CSB) incorporated with 15% oat bran. As a result, the single enzyme can significantly improve the specif...

Background: The processing of sweet potatoes generates a waste by-product rich in sweet potato protein (SPP). Objective: In this study, the effects of the concentrations of Alcalase and Ficin, hydrolysis time and pH value on the foaming properties of SPP hydrolysates (SPPH) determined via gas sparging method were investigated. Results: The res...

Background Zein is the major protein in corn and the main byproduct of the starch industry and, therefore, crucial for global agricultural commodities. Its unique fibre-forming properties, which underline its potential as a biodegradable material, have drawn intensified interest from the public, scientists, and industries because of growing concern...

Adipogenesis is a complex physiological process involving the formation of adipocytes and accumulation as adipose tissues. It is one of the contributors for the development of obesity. This study assessed the potential of phenolic extracts and potassium hydroxycitrate, obtained from Hibiscus sabdariffa, to inhibit adipogenesis. The phenolic extract...

Turmeric (Curcuma longa L.) powder is widely used as a spice and seasoning in Asian countries. This study investigated the effect of turmeric extracts on the anticancer activity of Huh7 and HCT 116 cells. The curcumin bioactive compounds were extracted using various methods such as microwave-assisted extraction (MAE), ultrasound-assisted extraction...

The manuscript investigates and reports for the first time utilizing a non-GMO approach to alter the fermentation process of Pinot Noir wines. We have experimentally demonstrated that certain dietary compounds possess histone deacetylase (HDAC) inhibiting activity and can alter the wine characteristics by potentially altering yeast gene transcripti...

Inspired by the emulsion stability of sugar beet pulp pectin, the hydrophobic protein fraction in sugar beet pulp (SBP) is expected to feature high interfacial activity. This work retrieved alkaline extracted protein-polysaccharide conjugates (AEC) from partially depectinized SBP by hot alkaline extraction. AEC was protein-rich (57.20 %), and the p...

This study reports the digestibility and nutritional quality of pasta made from durum wheat semolina which was partially substituted by puree, juice or pomace from spinach and red cabbage. The results show that 10% substitution of semolina with red cabbage pomace and spinach pomace, 1% substitution of spinach juice, and 2% substitution of spinach p...

Commercial emulsions are usually stabilized by multi-component formulations, such as particle and biopolymer, rather than single emulsifiers. However, their interactions and contributions to emulsification are complicated and lack elucidations. This work investigates the interaction between cellulose nanocrystal (CNC) and sugar beet pectin (SBP) an...

Edible hemp products or superfood refers to Cannabis sativa or industrial hemp. In general, hemp is a rich source of functional metabolites, such as Tetrahydrocannabinol (THC), Cannabidiol (CBD), and other cannabinoids. Hemp has been widely used in food products, such as bread, cookies, meatballs, energy bars, cooking oil, snacks, and crackers. Hem...

Herein, effects of astaxanthin usage on some quality parameters of meatballs and heterocyclic aromatic amines (HAAs) formation were investigated. The water content of meatballs with 1% of astaxanthin exhibited significantly (P<0.05) lower water content than other groups. The pH values of the samples were significantly (P<0.05) reduced due to astaxa...

Dietary phenolic compounds must be released from the food matrix in the gastrointestinal tract to play a bioactive role, the release of which is interfered with by food structure. The release of phenolics (unbound and bound) of cold and hot extruded noodles enriched with phenolics (2.0%) during simulated in vitro gastrointestinal digestion was inve...

To predict the maturity of bananas, the present study used non-destructive methods to analyze changes in the sweetness and color of the stalks, middles, and tips of bananas during ripening. The results indicated that the respective maturation of these three segments did not occur simultaneously, as indicated by the differential enzyme activity and...

A high glycaemic index diet would cause a rapid increase in blood sugar levels and lead to chronic metabolic disorders. In this study, wheat bread enriched with mushroom (Pleurotus eryngii and Cantharellus cibarius) powder at different content (0, 5, 10, and 15 g/100 g replacement based on total weight) was prepared. The effect on the physical, che...

Jiuzao glutelin (JG) properties were investigated in a previous study. To enhance the stability and application of JG in food products, JG was grafted with dextran, gum Arabic (GA), and pectin via Maillard reaction (MR) in this study. A nano-encapsulated carrier for riboflavin (RIB) and rhein (RHE) co-delivery was constructed based on the polysacch...

In this study, microencapsulation was used on gurum seed oil (GSO) to evaluate quality characteristics during accelerated storage at 60°C for 28 days. Accelerated storage altered the levels of all fatty acids of GSO (31.36‐ 95.65%), whereas, those of microencapsulated GSO showed reduced changes (0.13‐ 38.46%). At the beginning of storage, the tocop...

Epigenetic changes in genomics provide phenotypic modification without DNA sequence alteration. This study shows that benzoic acid, a common food additive and known histone deacetylase inhibitor (HDACi), has an epigenetic effect on Saccharomyces cerevisiae . Benzoic acid stimulated formation of epigenetic histone marks H3K4Me2, H3K27Me2, H3K18ac an...

Pullulan-Jiuzao glutelin (JG) conjugates (PJCs) were prepared via Maillard reaction in this study. PJCs were prepared by optimizing pullulan to JG ratios (0.5:1, 1:1, 2:1, and 4:1, expressed as PJC-0.5, PJC-1, PJC-2, and PJC-4, respectively) and reaction times (0-180 min) at pH 7 and 11. The secondary structure changes of PJC compared to JG, potent...

Wheat flour (WF) was substituted with different level of quinoa core flour (QCF), quinoa whole flour obtained by grinding mill (GQWF) and flour mill (RQWF) equipment separately, to develop QCF, GQWF and RQWF-formulated fried-free instant noodles. Tensile properties and quality attributes of dough, and cooking qualities, texture properties and senso...

This study aimed to develop and evaluate a method for simultaneously analyzing malondialdehyde (MDA) and two typical toxic α,β-unsaturated aldehydes, 4-hydroxy-2-hexenal (HHE), and 4-hydroxy-2-nonenal (HNE), using ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). The method possessed widely lin...