Changhui Zhao

Changhui Zhao
Jilin University | JUT · Department of Food Quality and Safety (College of Food Science and Engineering)

Ph.D

About

67
Publications
11,524
Reads
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964
Citations
Additional affiliations
September 2019 - August 2020
Jilin University
Position
  • Professor (Associate)
December 2015 - September 2019
Jilin University
Position
  • Lecturer
December 2015 - September 2019
Jilin University
Position
  • Lecturer
Education
January 2012 - May 2015
University of Maryland, College Park
Field of study
  • Nutrition and Food Science
September 2005 - July 2009
Harbin Institute of Technology
Field of study
  • Food Science and Engineering

Publications

Publications (67)
Article
Selenium is a trace nutrient that has both nutritional and nutraceutical functions, whereas narrow nutritional range of selenium intake limits its use. Selenium nanoparticles (SeNPs) are less toxic and more bioavailable than traditional forms of selenium, suggesting that SeNPs have the potential to replace traditional selenium in food industries an...
Article
Genetic modification has great advantages in improving performance of bacteria, but its oral safety has not been systematically evaluated. In this study, the toxicity including the reproductive toxicity of two genetically modified bacteria engineered using food-grade vectors on three generations of rats (F0, F1 and F2)were studied. Sprague Dawley r...
Article
Full-text available
Gynostemma pentaphyllum (G. pentaphyllum) is a perennial liana herb of the Cucurbitaceae family which has both nutraceutical and pharmacological functions. The objective of the current study was to investigate the preventative effects of G. pentaphyllum and Gypenoside-IV (GP-IV, a saponin monomer in G. pentaphyllum) on metabolic symptoms in high fa...
Article
Full-text available
The tiger nut is the tuber of Cyperus esculentus L., which is a high-quality wholesome crop that contains lipids, protein, starch, fiber, vitamins, minerals and bioactive factors. This article systematically reviewed the nutritional composition of tiger nuts; the processing methods for extracting oil, starch and other edible components; the physioc...
Article
Forest frog (Rana chensinensis) eggs contain high-quality protein but have not been well utilized. In this study, the total protein of forest frog eggs was extracted and 4491 protein/peptides were identified by HPLC-MS/MS. The egg protein was glycated using monosaccharides (lactose, fructose, xylose and glucose). The xylose modified egg protein sho...
Article
Lactoferrin (LF) shows good anticancer biological activity. Lactoferrin can combine with chlorogenic acid (CGA) in dairy drinks like milk coffee and milk tea. However, the nature and functional changes of LF after binding of CGA are not clear. The stability and binding properties of CGA on LF and its antiproliferative effect on colorectal cancer SW...
Article
Nucleic‐Acid‐Modified Liposome In article number 2101246, Hao Yu, Wentao Xu, and co‐workers review the construction strategies including covalent methods, non‐covalent methods, and protein‐mediated methods for fabricating nucleic‐acidmodified liposomes, and their applications in stability enhancement, biosensing, drug delivery and synthesis of biom...
Article
Liposomes with excellent biocompatibility have been widely used in nutraceutical and biomedical fields for delivery of bioactive molecules. Functional nucleic acids including aptamers that have good target recognizing ability and molecular assembly properties can be used to modify liposomes to expand their applications. This review comprehensively...
Article
Continuing studies show that whey components, notably proteins, have valuable nutritional and techno-functional properties supporting their applications in foods and drinks. Complementing these features is the increasing evidence of the health-promoting characteristics of whey proteins and peptides. This paper reviews whey production, composition,...
Article
Dongbei Suancai (DBSC) – a Chinese cabbage-based sauerkraut is a traditional fermented food which is popular in Asian countries. The biogenic amines that are usually generated during spontaneous fermentation have raised public health concern, while inoculation technology may solve this problem. In the current research, the biogenic amines, as well...
Article
Background Probiotics are a group of bacteria that play a critical role in intestinal microbiota homeostasis and may help adjunctively treat certain diseases like metabolic and immune disorders. Objective We recently generated a space-flight mutated Lactobacillus plantarum SS18-50 with good in vitro probiotic characteristics. In the current resear...
Article
Long-term consumption of a high-fat diet (HFD) can cause glucose and lipid metabolism disorders, damage the brain and nervous system and result in cognitive impairment. The objective of this study was to investigate the preventative effects of Lactobacillus paracasei (Jlus66, a probiotic extracted from cheese in Northeast China) on cognitive impair...
Article
Hericium erinaceus polysaccharides (HEPs) were isolated from the fruiting bodies of H. erinaceus with 53.36 % total carbohydrates and 32.56 % uronic acid. To examine whether HEPs can alter the diversity and the abundance of gut microbiota, juvenile and adult mice were fed with HEPs for 28 days. Based on the result of 16S sequencing of gut microbiot...
Article
As a dietary polyphenol, kaempferol exhibits numerous biological activities such as antioxidant and anticancer properties. However, its application is limited because of its poor solubility and low permeability. This work aims to investigate the interaction of kaempferol with α-lactalbumin. Multiple-spectroscopic techniques were used to prove the i...
Article
Revealing the interaction mechanism between bovine lactoferrin (LF) and 20(S)-ginsenoside Rg3 (Rg3), thereby introducing Rg3 to LF and even into stable emulsions will contribute significantly to food valorization and food industry. Adding Rg3 to LF caused slight absorbance increment and static fluorescence quench of LF, implying the successful comb...
Article
Selenium is an essential trace element that is involved in the synthesis of various selenoproteins in higher animals including humans. Twenty five selenoproteins have been identified in humans. These selenoproteins have various physiological functions. Organic selenium is safer than inorganic selenium and can be used as a nutritional supplement for...
Article
The purpose of the current study was to characterize the structural and functional properties of the hydrolysate of velvet antler heated at different temperatures (90, 100, 110 and 120 ℃) for 15 min. After heat treatment, the surface hydrophobicity of the velvet antler hydrolysates increased from 663.12 to 839.63 (at 100 ℃), indicating the maximum...
Article
Full-text available
The function and application of β‐glucosidase attract attention nowadays. β‐glucosidase was confirmed of transforming ginsenoside Rb1 to rare ginsenoside, but the interaction mechanism remains not clear. In this work, β‐glucosidase from GH1 family of Paenibacillus polymyxa was selected, and its gene sequence bglB was synthesized by codon. Then, rec...
Article
Tea polyphenols have become one of the promising healthcare molecules due to their anti-cancer, anti-oxidant, and anti-obesity capacities, making tea among the most popular and functional beverages worldwide. However, the sensitive and simple detection and discrimination of tea polyphenols is difficult to achieve since the analogues usually have si...
Article
Full-text available
Space mutation is an efficient tool in microbial breeding. The aim of the present study was to screen out space‐induced mutants of Lactobacillus plantarum with potent probiotic properties. The wild‐type Lactobacillus plantarum GS18 was subjected to 31 days and 18.5 hr of space flight, in which 13 isolates were selected for analysis. These mutants w...
Article
Whey protein is a fraction of milk protein that has both nutritional and health boosting effects. Hydrolyzation of whey protein by enzyme or fermentation can generate bioactive peptides that are beneficial to the life activities and/or regulate the physiological functions. Though whey protein has been widely applied in food industry, many newly dev...
Article
Background chlorogenic acid is a type of phenolic acid found in many plants. Chlorogenic acid has anti‐obesity effect with unclear mechanism. The current study was to investigate the regulatory effect of chlorogenic acid on energy balance in high fat diet induced obese C57BL/6J mice administrated with 100 mg/kg chlorogenic acid for 13 weeks. Resul...
Article
In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI–dextran conjugates (WPI‐D). The results of the extent of glycation (EG) and sodium...
Article
Food enriched with organic selenium is considered as a good source for selenium supplementation. In the current research, we cultivated Pleurotus citrinopileatus with medium containing different levels of sodium selenate, evaluated the antioxidant activity, and sequenced the transcriptome of the Se-enriched mushroom. Selenium content in Se-enriched...
Article
Glycosylation is one of the more studied methods used to improve the emulsification properties of proteins, and glycosylation products are often used as transport carriers for nutrients. In this study, we investigated the structure and emulsifying properties of whey protein isolate (WPI)-sugar conjugates formed with different sugars (glucose, lacto...
Article
Flaxseed oil is a type of herbal oil obtained from the ripened seeds of the flax plant (Linum usitatissimum L.) that is widely utilized for baked foods. The aim of present study was to investigate the effect of flaxseed oil on ulcerative colitis. The rats were gavaged daily with flaxseed oil at doses of 400, 800 and 1600 mg/kg b.w. for six weeks, w...
Article
Glyphosate tolerant soybeans represent a large portion of soybeans grown and fed to farm animals around the world. Despite their widespread use for many years, some have raised questions regarding their safety because the soybeans were genetically modified. The CP4 EPSPS gene which imparts resistance to topical application of the herbicide glyphosa...
Article
Full-text available
The nonalcoholic fatty liver disease (NAFLD) is a progressive liver disease that affects the health of people in an increasing rate. In the current research, we investigated the beneficial effect of a novel probiotic strain L. paracasei Jlus66 (Jlus66) on rats with high‐fat diet (HFD)‐induced NAFLD. The intestinal flora of rats was analyzed based o...
Article
The ovum oil of forest frog has various health beneficial functions. In the current research, we evaluated the hypolipidemic effects of the low-cholesterol ovum oil from the forest frog and its combination with stigmasterol in rats.
Article
In the current research, we systematically investigated the physicochemical stability of the nanoemulsions that were formulated with 90% (v/v) whey protein isolate (WPI) aqueous phase and 10% (v/v) medium chain triglyceride oil phase at neutral pH using high intensity ultrasonication. We measured the physicochemical properties of nanoemulsions stab...
Article
Full-text available
Obesity has become one of the most important health problems worldwide requiring urgent need for efficient control. Pleurotus citrinopileatus (P. citrinopileatus)—a type of edible mushroom with abundant bioactive molecules—is a promising source for achieving this goal. In the present study, we evaluated the anti‐obesity and hypolipidemic effect of...
Chapter
Whey protein is one of the two major protein types of bovine milk, accounting for 20 percent of milk protein, while casein accounts for the remaining 80 percent. Human breast milk contains less methionine, phenylalanine, and lysine compared to bovine milk and more cysteine and tryptophan. Branched chain amino acids (BCAAs) are highly valued amino a...
Article
Full-text available
Hericium erinaceus (HE) is a well-known edible and medicinal fungus widely grown in Asian countries. Polysaccharides from the Hericium erinaceus (HEP) are major biological macromolecules. It has been reported that HEP has multiple biological activities, such as antioxidant activity, immunomodulatory effects, anti-inflammatory effect, anti-chronic g...
Article
Full-text available
Background Previous studies suggested that probiotics intervention may be one of the methods for preventing and/or treating gastric ulcer. Objective The aim of the study was to compare the preventive effects of a spaceflight mutant Lactobacillus reuteri F-9-35 and its wild type on ethanol-induced gastric injury in rats. Design Forty rats were ran...
Article
Procyanidins are a group of flavonoids that can be found in many plants. The objective of the current study was to investigate the anti-obesity effect of procyanidin on high fat diet induced obese C57BL/6J mice. The mice were administrated with 100 mg/kg procyanidin for 12 weeks. The results showed that consumption of procyanidin significantly amel...
Article
Full-text available
Beta‐carotene, the main dietary source of provitamin A, is required for maintaining optimum human health. The bioaccessibility of beta‐carotene can be greatly improved when ingested with fat. Therefore, the aim of the current study was to select proper oils (palm oil, coconut oil, fish oil, and corn oil) as a carrier to form stable nanoemulsion tha...
Article
Probiotics are considered to be a potential treatment for ulcerative colitis (UC). The aim of this study was to compare the preventive effect of a space flight-induced mutant L. reuteri F-9-35 and its wild type on UC in vivo. Female mice were randomly assigned to five groups: one normal and four colitic. Mice from colitis groups were daily gavaged...
Article
Full-text available
Thermal treatment has been utilized to improve the functional properties of proteins for many years. In this study, we aimed to investigate the effect of sodium triphosphate (Na5P3O10) on particle size and size distribution of heat‐induced whey protein concentrate (WPC) aggregates under different processing conditions. The results showed that high...
Article
Full-text available
With the rapid development of modern society, many chronic diseases are increasing including diabetes, obesity, cardiovascular diseases, etc., which further cause an increased death rate worldwide. A high caloric diet with reduced natural polysaccharides, typically indigestible polysaccharides, is considered a health risk factor. With solid evidenc...
Article
Purple lettuce (Lactuca sativa L.) is a type of low caloric vegetable often consumed freshly, whereas its function in metabolic control has been little studied. To investigate the protective effect of a type of purple lettuce against metabolic risk factors, we treated the C57BL/6J DIO mice with or without purple lettuces for 8 weeks. Results showed...
Article
Full-text available
Effect of ultrasound treatment on the physicochemical properties and structure of β‐lactoglobulin were investigated. β‐Lactoglobulin was treated with ultrasound at different amplitudes, temperatures, and durations. The surface hydrophobicity and free sulfhydryl group of β‐lactoglobulin were significantly increased after ultrasound treatment (p < .0...
Article
Full-text available
Alliin (S-allyl cysteine sulfoxide) is a bioactive sulfoxide compound derived from garlic. To evaluate the preventive effect of alliin against metabolic risk factors in diet induced obese (DIO) mice, we treated the C57BL/6J DIO mice with drinking water with or without alliin (0.1 mg/ml) for 8 weeks. Results showed that alliin had no significant eff...
Article
Full-text available
Lutein is a hydrophobic carotenoid that has multiple health functions. However, the application of lutein is limited due to its poor solubility in water and instability under certain conditions during storage. Hereby we generated lutein loaded nano-emulsions using whey protein isolate (WPI) or polymerized whey protein isolate (PWP) with assistance...
Article
Astaxanthin nanodispersion was prepared using whey protein isolate (WPI) and polymerized whey protein (PWP) through an emulsification–evaporation technique. The physicochemical properties of the astaxanthin nanodispersion were evaluated and the transport of astaxanthin was assessed using a Caco-2 cell monolayer model. The astaxanthin nanodispersion...
Article
Nonalcoholic fatty liver disease (NAFLD) is the main cause of chronic liver disease worldwide. Previous evidence indicates that probiotics can be applied as therapeutic agent for NAFLD. In this study, the potential probiotic strain Lactobacillus paracasei Jlus66 was isolated from natural fermented milk using a culture-dependent method, and its prob...
Article
Heat treatment is a common method to improve certain functional properties of whey protein, whereas this process is usually accompanied with the formation of lysinoalanine (LAL), which may reduce the protein nutritional value and even cause safety concerns. This study aimed to investigate the effect of the protein content (8–10%, wt/vol), pH (7.5–8...
Article
Pre-heated whey protein isolate (WPI) solution (10% w/v, 85°C for 30 min) was prepared and subjected to high-intensity ultrasound (HUS, 20 kHz) at different durations (5-40 min) before acidification to determine the effects of HUS on glucono-δ-lactone (GDL)-induced gelation properties of whey protein gel. Results showed that HUS reduced the particl...
Article
Purple sweet potato (PSP) is widely grown in Asia and considered as a healthy vegetable. The objective of the current study was to determine the anti-obesity effect of the PSP on high fat diet induced obese C57BL/6J mice. The mice were administrated with high fat diet supplemented with the sweet potato (SP) or PSP at the concentration of 15% and 30...
Article
Pleurotus citrinopileatus is an edible medicinal mushroom rich in biomolecules and thus has a high potential for use in formulating pharmaceutical and nutraceutical products. To test its effect on body weight and glucose control, we generated diet-induced obese (DIO) C57BL/6J male mice by feeding the mice a high-fat diet (60% fat) for 8 weeks and t...
Article
Full-text available
Selenium-binding protein 1 (SBP1) is not a selenoprotein but structurally binds selenium. Loss of SBP1 during carcinogenesis usually predicts poor prognosis. Because genome instability is a hallmark of cancer, we hypothesize that SBP1 sequesters cellular selenium and sensitizes cancer cells to DNA-damaging agents. To test this hypothesis, we knocke...
Article
Energy balance is achieved as the result of both central and peripheral regulators working together. Sugar intake can cause a disruption in energy balance that can increase the risk of the onset of obesity. In this review, we will discuss the relationship between sugar consumption and obesity. We have summarized the current evidence that shows how...
Article
The dicamba-tolerant soybean MON87708 expresses the dicamba mono-oxygenase (DMO) enzyme that is encoded by the dmo gene. In order to evaluate the safety of this soybean, a 90-day subchronic feeding toxicity study (13 weeks) was conducted on Sprague-Dawley rats. A total of 140 rats were divided into 7 groups (10/sex/group), including a standard comm...
Chapter
Selenium binding protein 1 (SBP1) is a ubiquitously expressed protein that can bind selenium specifically. A prominent feature of SBP1 is its downregulation during tumorigenesis of various origins. SBP1 is also involved in senescence, the stress response, cellular differentiation, protein transport, and the ubiquitinating/deubiquitinating pathways....
Article
Lipid rafts are microdomains in plasma membrane and can mediate cytotoxicity. In this study, the role of lipid rafts in ochratoxin A-induced toxicity was investigated using Hepatoblastoma Cell Line HepG-2 cells. Disruption of cholesterol-containing lipid rafts enhanced Ochratoxin A (OTA) toxicity, as shown by increased lactate dehydrogenase leakage...
Article
Full-text available
Fructose can quickly increase the 11β-HSD1 expression in both the liver and adipose tissue of rats. The intra cellular glucocorticoids regeneratedby11β-HSD1 increase the risk of obesity development. However, how sugars affect the expression of hypothalamic 11β-HSD1 is not clear. In the current paper, we treated Sprague Dawley rats with 15% of diffe...
Article
Oxidative stress and apoptosis are involved in Ochratoxin A (OTA)-induced renal cytotoxicity. Apoptosis signal-regulating kinase 1 (ASK1) is a Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK, MAP3K) family member that plays an important role in oxidative stress-induced cell apoptosis. In this study, we performed RNA interference of ASK1 in H...
Article
Full-text available
It has been known for years that free access to sugar solutions can cause weight gain and/or obesity in rats. We recently reported that brief access to sugar solutions can affect the hypothalamic neuropeptides that help to regulate energy balance. In this paper, we present the results in which we examined the effects of these sugars on the expressi...
Article
It has been known for years that free access to sugar solutions can cause weight gain and/or obesity in rats. We recently reported that brief access to sugar solutions can affect the hypothalamic neuropeptides that help to regulate energy balance. In this paper, we present the results in which we examined the effects of these sugars on the expressi...
Thesis
Long term use of sugars can induce excess caloric intake and/or obesity. To evaluate the effects of sugar intake on different regions of the hypothalamus (the brain's control center for energy homeostasis) we first developed and then evaluated a microscope-assisted dissection method. Because of the small size of the paraventricular nucleus, we vali...
Article
Many Chinese herbs are traditionally used as medicine to improve the functions of gastrointestinal tract. Some of these herbs are also promising agents for the improvement of the gut microbiota and the treatment of ulcerative colitis. By screening seven traditional Chinese herbs, we found that Red Ginseng and Semen Coicis were the most effective in...
Article
Full-text available
Ochratoxin A (OTA) is known to be nephrotoxic and hepatotoxic in rodents when exposed orally. To understand the systematic responses to OTA exposure, GC-MS- and (1)H-NMR-based metabolomic techniques together with histopathological assessments were applied to analyse the urine and plasma of OTA-exposed rats. It was found that OTA exposure caused sig...
Article
Full-text available
The objective of this work was to characterize the fat globule and milk fat globule membrane (MFGM) proteins in Maiwa (n = 6), Gannan (n = 4) and Zhongdian yak whole milks (n = 4). Fat globule size distribution and specific fat surface area of whole yak milk were determined using a laser diffraction particle size analyzer. The average specific fat...
Article
To obtain functional yeast with antioxidant ability for food industry, the antioxidant activity of intact cell and intracellular cell-free extract of Pichia fermentans BY5 and Issatchenkia orientalis BY10 was investigated. Both intact cell and extract of them demonstrated antioxidant activity ranged from 49% to 68%. The ability to scavenge 1, 1-dip...

Projects

Projects (6)