Chang Chen

Chang Chen
Wageningen University & Research | WUR · Laboratory of Physical Chemistry and Soft Matter

Master of Engineering

About

22
Publications
3,887
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351
Citations
Introduction
Mr. Chen completed his bachelor's degree at Harbin Institute of Technology (HIT) from 2012 to 2016. After that, He came to HIT, Shenzhen (HITsz) and got his master degree in January 2019. From January 2020, he worked as a research assistant at HITsz and Peking university until May 2021. Now he is a PhD student at Wageningen University & Research. His PhD project is about the bioengineering of cell-mimicking vesicles that can undergo shape transformations and primitive migration.
Skills and Expertise

Publications

Publications (22)
Article
Microfluidic paper-based analytical devices (µPADs) have been extensively studied for disease diagnostics, food quality control and environmental monitoring due to the advantages of low cost, portability, and simplicity. The lack of flow con-trollability has triggered the development of valves for such devices. This paper reports the µPADs integrat...
Article
Inoculation of single cells into separate culture chambers is one of the key requirements in single-cell analysis. This paper reports an innovative microfluidic chip integrating two pneumatic microvalves to screen and print single cells onto a well plate. The upper and lower size limits of cells can be dynamically controlled by regulating the defor...
Article
Paper microfluidics has been extensively exploited as a powerful tool for environmental and medical detection applications. Both flow delay and compatibility with either polar or non-polar reagents are indispensable for the automation of detections requiring multiple reaction steps. This article reports the systematic studies of a 3D-printing proto...
Article
Full-text available
Generating and maintaining the concentration dilutions of diffusible molecules in microchannels is critical for high-throughput chemical and biological analysis. Conventional serial network microfluidic technologies can generate high orders of arbitrary concentrations by a predefined microchannel network. However, a previous design requires a large...
Article
Full-text available
Compartmentalization is a vital aspect of living cells to orchestrate intracellular processes. In a similar vein, constructing dynamic and responsive sub-compartments is key to synthetic cell engineering. In recent years, liquid-liquid phase separation via coacervation has offered an innovative avenue for creating membraneless organelles (MOs) with...
Article
Full-text available
Particle‐stabilized emulsions, also known as Pickering emulsions, have shown promise in areas that require long‐term stability with minimum use of surfactants. While most work has focused on densely covered Pickering emulsions, such emulsions are known to retain stability even when the interfaces are sparsely covered with particles. Here, the forma...
Article
Full-text available
Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid transition is currently not understood. This study focuses on the phase transitions of a disordered glycine-rich protein (GRP) found in tick saliva...
Preprint
Full-text available
Living cells orchestrate a myriad of biological reactions within a highly complex and crowded environment. A major factor responsible for such seamless assembly are the preferential interactions between the constituent macromolecules, either associative or segregative, that can drive de-mixing to produce co-existing phases, and thus provide a dynam...
Article
Full-text available
Pathological conditions linked to shear stress have been identified in hematological diseases, cardiovascular diseases, and cancer. These conditions often exhibit significantly elevated shear stress levels, surpassing 1000 dyn/cm² in severely stenotic arteries. Heightened shear stress can induce mechanical harm to endothelial cells, potentially lea...
Article
An important exercise for understanding the emergent behavior in biology is to study it using minimal synthetic systems. Current biomimetic systems involving directed motion and collective migration require at least three components: two immiscible solvents to form an emulsion, and external agent(s), usually surfactants, to give rise to asymmetric...
Preprint
Full-text available
Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid transition is not yet understood. This study focuses on the phase transitions of a disordered glycine-rich protein (GRP) that is prominent in tick...
Article
Full-text available
Biosensing using liquid crystals has a tremendous potential by coupling the high degree of sensitivity of their alignment to their surroundings with clear optical feedback. Many existing set-ups use birefringence of nematic liquid crystals, which severely limits straightforward and frugal implementation into a sensing platform due to the sophistica...
Preprint
Full-text available
Biosensing using liquid crystals has a tremendous potential by coupling the high degree of sensitivity of their alignment to theirsurroundings with clear optical feedback. Many existing set-ups use birefringence of nematic liquid crystals, which severelylimits straightforward and frugal implementation into a sensing platform due to the sophisticate...
Article
Full-text available
Microfluidic paper-based analytical devices (μPADs) have been widely explored for point-of-care testing due to their simplicity, low cost, and portability. μPADs with multiple-step reactions usually require precise flow control, especially flow-delay. This paper reports the numerical, mathematical, and experimental studies of flow delay through wax...
Article
Full-text available
Printing single cells into individual chambers is of critical importance for single-cell analysis using traditional equipment, for instance, single-cell clonal expansion or sequencing. The size of cells can usually be a reflection of their types, functions, and even cell cycle phases. Therefore, printing individual cells within the desired size ran...
Article
Shear stress of physiological flows in the human body plays a critical role in maintaining the structure and functions of single cells and multicellular organs. Recently, the wide-spreading microfluidic technologies have made significant contributions to the in-vitro recapitulation of complex biochemical and mechanical cues. Microfluidic approaches...
Article
It is critical to reliably and rapidly detect multiple disease biomarkers in tiny liquid samples with high sensitivity to meet the growing demand for point-of-care diagnostics. This paper reports a microfluidic platform integrating magnetic-based single bead trapping in conjunction with acoustic micromixing for simultaneous detection of multiple ca...

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