• Home
  • Chandradhish Ghosh
Chandradhish Ghosh

Chandradhish Ghosh
Helmholtz Institute for RNA based infection Research · RNA Biology of Bacterial Infections

Doctor of Philosophy

About

31
Publications
9,052
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,868
Citations
Additional affiliations
January 2017 - February 2021
Max Planck Institute of Colloids and Interfaces
Position
  • PostDoc Position
Description
  • Glyconanomaterials as drug carriers and immunotherapeutics; Automated glycan assemby; Drug sugar conjugates; Nanobody drug conjugates.
May 2009 - July 2009
Indian Institute of Science Bangalore
Position
  • Research Assistant
December 2010 - present
Jawaharlal Nehru Centre for Advanced Scientific Research
Position
  • PhD Student

Publications

Publications (31)
Article
The international symposium ASOBIOTICS 2024 brought together scientists across disciplines to discuss the challenges of advancing antibacterial antisense oligomers (ASOs) from basic research to clinical application. Hosted by the Helmholtz Institute for RNA-based Infection Research (HIRI) in Wurzburg, Germany, on September 12-13th, 2024, the event...
Article
Full-text available
Antisense oligomer (ASO)-based antibiotics that target mRNAs of essential bacterial genes have great potential for counteracting antimicrobial resistance and for precision microbiome editing. To date, the development of such antisense antibiotics has primarily focused on using phosphorodiamidate morpholino (PMO) and peptide nucleic acid (PNA) backb...
Preprint
Full-text available
Antisense oligomer (ASO)-based antibiotics that target mRNAs of essential bacterial genes have great potential for counteracting antimicrobial resistance and for precision microbiome editing. To date, the development of such antisense antibiotics has primarily focused on using phosphorodiamidate morpholino (PMO) and peptide nucleic acid (PNA) backb...
Article
Membrane-active small molecules (MASMs) are small organic molecules designed to reproduce the fundamental physicochemical properties of natural antimicrobial peptides: their cationic charge and amphiphilic character. This class of compounds has a promising broad range of antimicrobial activity and, at the same time, solves some major limitations of...
Article
Full-text available
Nanoparticles that modulate innate immunity can act as vaccine adjuvants and antigen carriers and are promising alternatives to conventional anticancer therapy. Nanoparticles might, upon contact with serum, activate the complement system that might in turn result in clearance and allergic reactions. Herein, we report that ultrasmall glyconanopartic...
Article
Full-text available
Infections by intracellular pathogens cause significant morbidity and mortality due to lack of efficient drug delivery. Amphotericin B, currently used to treat leishmaniasis and cryptococcosis, is very toxic and cannot eradicate intracellular Cryptococcus neoformans (C. neoformans). Glycosylated gold nanoparticles are water dispersible and biocompa...
Article
Full-text available
Malaria, a mosquito-borne disease caused by the Plasmodium species, claims more than 400,000 lives globally each year. Increasing drug resistance of the parasite renders the development of new anti-malaria drugs necessary. Alternatively, better delivery systems for already marketed drugs could help to solve the resistance problem. Herein, we report...
Article
Full-text available
Clostridium difficile infections (CDIs) are a growing health concern worldwide. The recalcitrance of C. difficile spores to currently available treatments and concomitant virulence of vegetative cells has made it imperative to develop newer modalities of treatment. Aryl-alkyl-lysines have been earlier reported to possess antimicrobial activity agai...
Article
As more antibiotics are rendered ineffective by drug-resistant bacteria, focus must be shifted towards alternative therapies for treating infections. Although several alternatives already exist in nature, the challenge is to implement them in clinical use. Advancements within biotechnology, genetic engineering, and synthetic chemistry have opened u...
Article
The emergence of bacterial resistance and hesitance in approving new drugs has bolstered research on membrane-active agents such as antimicrobial peptides and their synthetic derivatives as therapeutic alternatives against bacterial infections. Herein, we document the action of aryl-alkyl-lysines on liposomes mimicking bacterial membranes using sol...
Article
L-lysines were conjugated to lipidated biphenyls using simple synthetic chemistry to obtain selective membrane-active antibacterial agents that inhibited cell-wall biosynthesis. The most selective compound bore promising activity against biofilm-related infections and intracellular bacteria, but was belligerent to resistance development. Further, i...
Article
Mortality due to pathogenic fungi has been exacerbated by the rapid development of resistance to frontline antifungal drugs. Fungicidal compounds with novel mechanisms of action are urgently needed. Aryl-alkyl-lysines, which are membrane-active small molecules, were earlier shown to be broad-spectrum antibacterial agents with potency in vitro and i...
Article
Cell wall biosynthesis inhibitors (CBIs) have historically been one of the most effective classes of antibiotics. They are the most extensively used class of antibiotics and their importance is exemplified by the β-lactams and glycopeptide antibiotics. However, this class of antibiotics has not received impunity from resistance development. In the...
Article
Due to emerging resistance there is a steady need for new antimalarial drugs. Here, we report a new class of water soluble, non-toxic compounds, aryl-alkyl-lysines, with promising activity against the ring stage of Plasmodium falciparum. The optimal compound perturbed the plasma membrane potential and the digestive vacuole of parasites. In the muri...
Article
Full-text available
In light of the recent outbreak of Ebola virus (EBOV) disease inWest Africa, there have been renewed efforts to search for effective antiviral countermeasures. A range of compounds currently available with broad antimicrobial activity have been tested for activity against EBOV. Using live EBOV, eighteen candidate compounds were screened for antivir...
Article
Full-text available
In the global effort to thwart antimicrobial resistance, lipopeptides are an important class of antimicrobial agents, especially against Gram-negative infections. In an attempt to circumvent their synthetic complexities, we designed simple membrane-active agents involving only one amino acid and two lipid tails. Herein we show that the use of two s...
Article
Bacterial colonization and subsequent formation of biofilms onto surfaces of medical devices and implants is a major source of nosocomial infections. Most antibacterial coatings to combat infections are either metal-based or non-degradable polymer-based and hence limited by their non-degradability and unpredictable toxicity. Moreover, to combat inf...
Article
Full-text available
Correction for 'Selective and broad spectrum amphiphilic small molecules to combat bacterial resistance and eradicate biofilms' by Jiaul Hoque et al., Chem. Commun., 2015, 51, 13670-13673.
Article
Full-text available
Development of synthetic strategies to combat Staphylococcal infections, especially those caused by methicillin resistant Staphyloccus aureus (MRSA), needs immediate attention. In this manuscript we report the ability of aryl-alkyl-lysines, simple membrane active small molecules, to treat infections caused by planktonic cells, persister cells and b...
Article
Full-text available
Infections caused by drug-resistant Gram-negative pathogens continue to be significant contributors to human morbidity. The recent advent of New Delhi metallo-β-lactamase-1 (blaNDM-1) producing pathogens, against which few drugs remain active, has aggravated the problem even further. This paper shows that aryl-alkyl-lysines, membrane-active small m...
Article
Full-text available
The emergence of bacterial resistance and biofilm associated infections has created a challenging situation in global health. In this present state of affairs where conventional antibiotics are falling short of being able to provide a solution to these problems, development of novel antibacterial compounds possessing the twin prowess of antibacteri...
Article
Bacterial biofilms represent the root-cause of chronic or persistent infections in humans. Gramnegative bacterial infections due to nosocomial and opportunistic pathogens such as Acinetobacter baumannii are more difficult to treat because of their inherent and rapidly acquiring resistance to antibiotics. Due to biofilm formation, A. baumannii has b...
Article
Infectious diseases continue to be one of the major contributors to human morbidity. The rapid rate at which pathogenic microorganisms have developed resistance against frontline antimicrobials has compelled scientists to look for new alternatives. Given their vast antimicrobial repertoire, substantial research effort has been dedicated toward the...
Article
Several antibiotics possess, beyond their conventional mechanism of antibacterial action, secondary functions which inhibit bacterial pathogenesis. One such secondary action is that of inhibiting quorum sensing in bacteria. Furthermore, there are certain drugs and natural molecules which do not possess antibacterial action but possess functions whi...
Article
Full-text available
Rationally designed amphiphilic small molecules kill selectively drug-sensitive and drug-resistant bacteria over mammalian cells. The small molecules disperse preformed biofilm and reduce the viable bacterial count in the biofilm. Moreover, this class of membrane-active molecules disarm the development of bacterial resistance.
Article
Full-text available
Vancomycin, a glycopeptide antibiotic, has long been a drug of choice for life-threatening Gram-positive bacterial infections. Vancomycin confers its antibacterial activity by inhibiting bacterial cell wall biosynthesis. However, over the time, vancomycin has also been rendered ineffective by vancomycin-resistant bacteria (VRB). These bacteria deve...
Article
Full-text available
Natural and synthetic membrane active antibacterial agents offer hope as potential solutions to the problem of bacterial resistance as the membrane-active nature imparts low propensity for the development of resistance. In this report norspermidine based antibacterial molecules were developed which displayed excellent antibacterial activity against...
Article
Full-text available
The emergence of multi-drug resistant bacteria compounded by depleting arsenal of antibiotics has accelerated efforts towards development of antibiotics with novel mechanism of action. In this report we present a series of small molecular antibacterial peptoid mimics which exhibit high in-vitro potency against a variety of Gram-positive and Gram-ne...

Questions

Question (1)
Question
I am conjugating a drug on gold nanoparticles. The unbound drug absorbs well between 350-400 nm. The naked gold nanoparticle absorbs between 250-500 nm. After conjugation of the drug to the nanoparticle, the UV features of the drug are skewed and blue shifted. I thought of creating a standard curve of the plain drug and calculate the amount of drug on the nanoparticle by correlation. However, because of this aforementioned problem, I do not know if it is a good idea to use the same technique.
The drug also shows fluorescence. If I take an excitation scan of the nanoparticle keeping fluorescence at 500 nm constant, the excitation spectra matches to that of unbound drug. Also exciting at 350 nm, the fluorescence spectra of the nanoparticles matches with that of the drug. Gold nanoparticles has absolutely no contribution in the fluorescence spectra. I proceeded to make a standard curve of fluorescence with different concentrations of the drug and calculate the amount of drug on the nanoparticle by correlation of fluorescence intensities. Is fluorescence a correct technique to use for quantification of the amount of drug? Any help would be appreciated.

Network

Cited By