Chandan Paswan

• PhD (Animal Genetics & Breeding)
• Researcher at Directorate of Poultry Research Hyderabad

Short term selection response for egg mass at 52 weeks of age (EM52), the primary trait of selection and the correlated response of other production traits was evaluated utilizing the last five generations data of Vanaraja female line (PD-2) at ICAR-Directorate of Poultry Research, Hyderabad. The phenotypic and genetic response for EM52 was signifi...

The present study was carried out to assess the inheritance of growth traits and to study the effect of selection on carcass characteristics and egg quality traits in Vanashree, an improved indigenous chicken. Estimates of heritability were high for body weights recorded at 4, 5 and 6 weeks of age and 8th week shank length, while it was moderate fo...

Breeding value (BV), genetic parameters and additive genetic, and maternal effects were evaluated on growth and production traits utilizing data from eight generations employing animal model in a rural male parent line (PD‐6) chicken at ICAR‐Directorate of Poultry Research, Hyderabad, India. The least squares means (LSM) for body weight (BW) and sh...

Variance and genetic parameters were estimated for growth and production traits of synthetic broiler female line (PB-2) using REML animal model to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits. The overall least squares mean (LSM) for bo...

The data on 837 birds representing five crosses (Aseel x PD-1, Aseel x PD-2, Aseel x PD-6, Aseel x PB-1 and Aseel x PB-2) survived up to 12 weeks of age were evaluated for various growth, meat quality, meat composition, sensory attributes and carcass traits as an alternative to native chicken farming. Body weights and shank lengths significantly (P...

Vanaraja female line (PD-2) chicken was evaluated for growth, production, carcass and egg quality traits utilizing the data generated from 3,737 chicks and 599 hens produced in pedigreed mating with 50 sires and 250 dams at ICAR-Directorate of Poultry Research, Hyderabad, Telangana. Fertility was 86.63% and the hatchability was 86.72% on fertile eg...

Background: Rural poultry farming with improved dual purpose chicken varieties is fast growing in the country. The meat quality of cocks and its acceptance by the consumer is important for its propagation as dual purpose variety. The two way cross produced by PD-1 xPD-4 (improved Aseel) was evaluated for growth, carcass and meat quality.Metohds: Th...

A comprehensive study was carried out in Gramapriya female line (PD-3) with respect to growth, production, selection response and population structure utilizing the data generated for the last 7 generations at ICAR-Directorate of Poultry Research, Hyderabad, Telangana. The average fertility was 71.89% and hatchability was 80.52% on fertile egg set...

Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken. Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five genera...

The present study was formulated to characterize and comprehend the molecular structural characteristics of ACTRIIB receptor in Aseel and control broiler (CB) populations. The full length coding sequence (1539 bp) of the receptor was amplified, cloned, sequenced and analyzed using bioinformatic tools. The physico chemical properties of protein and...

Molecular breeding exploiting candidate genes is burgeoning reproductive approach to improve growth traits in poultry. The activin type IIB receptor (ACTRIIB) is a negative growth regulator, modulating action of many muscle growth regulators. PCR Single strand conformation polymorphism was employed to unravel polymorphism in promoter region of the...

A comprehensive study was conducted to evaluate the performance of a two way cross developed for free range poultry farming under farm and field conditions. Chicks (412) produced by mating PD-1 males with PD-4 females in single hatch were utilized for the study. The data collected on 200 chicks from field and 193 chicks from farm were analysed to s...

Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA construct...

In the present study, we quantified myoglobin (Mb) gene expression in two different indigenous chicken breeds, namely Ghagus and Aseel to discern tissue specific expression pattern in different age groups. Quantitative real-time PCR assay was developed for measurement of the Mb mRNA expression in different tissues in chickens of different age group...

Birds (364) of both sexes, 11-wk-old, belonging to 2 native (Brown Nicobari and Ghagus) breeds and 1 exotic breed (Dahlem Red) were evaluated for cell-mediated immune response (CMI) by phytohemagglutinin-P (PHA-P), hemagglutination inhibition (HI) assay against Newcastle disease virus (NDV) antigen (LaSota stock virus), flow cytometric analysis of...

The growth and production performance of a promising three way cross was evaluated under farm and backyard conditions to assess its suitability as backyard chicken variety. The data generated on 200 birds from backyard and 136 birds from farm conditions was analyzed to study the effect of sex on growth and rearing system on growth and production tr...

The comparative performance of the terminal crosses involving either Poultry Directorate-6 (PD-6) or Poultry Directorate-2 (PD-2) lines as male and Poultry Directorate-3 (PD-3) line as female parents was evaluated to study the suitability of PD-6 line for production of Gramapriya rural chicken variety utilizing the performance data on 534 birds bel...

The North-Eastern Hills Region of India in general and Nagaland in particular is characterized by inaccessibility, marginality, fragility, cultural heterogeneity, rich biodiversity, low livestock and crop productivity. In Nagaland, there are 16 recognized tribes besides numerous minor tribes which are mostly non-vegetarian. Also, the per caput cons...

Aseel is an important native chicken breed of India, known for its martial qualities (aggressive fighting abilities), pugnacity, and majestic gait. The aim of the study is to conserve and characterize the Aseel germplasm, which is considered to be endangered. The birds were maintained on deep litter under a simulated backyard type of housing having...

Myostatin is a member of TGF-β super family and is directly involved in regulation of body growth through limiting muscular growth. A study was carried out in three chicken lines to identify the polymorphism in the coding region of the myostatin gene through SSCP and DNA sequencing. A total of 12 haplotypes were observed in myostatin coding region...

Augmenting the meat production is among the primary breeding objective of genetic selection programs in poultry production. However, the knowledge about the expression of genes regulating muscle growth at the molecular level is inadequate. Activin type IIB receptor (ACTRIIB) has been reported to play vital role in the negative regulation of muscle...

Amongst several trans-boundary diseases of small ruminants in India, Peste des petits ruminants (PPR) is a devastating disease due to its spread and economic importance. The main strategy for prevention of this disease is the vaccination with attenuated whole virus vaccine. Present study was carried out to identify the sources of variation and also...

A study was carried out to characterize the nucleotide variability in the promoter of the IGF-1 gene in broiler line of chicken. A PCR product of 375bp was amplified and nucleotide variability was studied using PCR-SSCP technique in chicken control broiler line. Selected sample PCR products were also sequenced to confirm the variability in promoter...

Carcass traits are of immense importance in chicken as some parts of the carcass particularly breast muscle and legs are mostly preferred by the consumers with good market price while the remaining parts have a lower price. Carcass traits primarily depend on the growth of the birds, which are controlled by the candidate genes. Activin receptor type...

Malpura sheep is one of the heavy mutton type sheep breeds found in semi-arid region of Rajasthan. Objective of the study was to revise the earlier selection criteria and set up a new one for Malpura sheep. The data on 2,995 Malpura sheep descended from 188 sires and 1,021 dams for 11 years (2004–2014) was used to construct the indices using variou...

Follistatin gene is a member of transforming growth factor-β super family involved in muscle growth. The present study was carried out to characterize the nucleotide variability in the exonic (coding) region of FSTN gene in boiler (PD-1) line and control layer chicken lines. A PCR product of 219 bp of exon-3 was amplified and nucleotide variability...

Ovine MHC DRB1 exon 2 (Ovar-DRB1.2) gene is arguably one of the critical genes, responsible for disease resistance against parasite in animals. This study was carried out for indepth analysis of polymorphism in MHC DRB1.2 gene and to explore the underlying molecular mechanisms for the development of disease resistance in Garole. PCR-RFLP study reve...

Activin Type 2A Receptor (ACVR2A) is the receptor for Myostatin, a member of TGFβ super family in chicken and other animals. Expression profile of ACVR2A gene was studied in two contrasting lines of chicken i.e control broiler (CB) having faster growth rate and control layer (CL) having slower growth. Expression pattern of ACVR2A gene during embryo...

1. The objectives of the study were to detect polymorphism in the coding region of the IGF1 gene, explore the expression profile and estimate association with growth traits in indigenous and exotic chickens. 2. A total of 12 haplotypes were found in Cornish, control layer and Aseel breeds of chicken in which the h1 haplotype was most frequent. 3. N...

Estimates of (co)variance components and genetic parameters were calculated for birth weight (BWT), weaning weight (WWT), 6 month weight (6WT), 9 month weight (9WT), 12 month weight (12WT), average daily gain (ADG) from birth to weaning (ADG1), weaning to 6 month (ADG2) and 6 to 12 month (ADG3) for Malpura sheep. Data were collected over a period o...

India although has achieved self sufficiency in food grains, it has to solve the problems of protein-energy malnutrition for millions of people. Presently, poultry industry has emerged as a big hope, which can meet not only the protein requirement to millions but can provide employment and thereby, livelihood security. India is the 3rd largest prod...

Myostatin (MSTN), a transforming growth factors β (TGF-β) family member, is an important regulator of muscle mass. While polymorphism in the coding region of MSTN is extensively studied, the genetic causes of transcriptional regulation are poorly understood. This study is targeted to identify variations in the promoter region of MSTN. This study re...

Malpura sheep is one of the well adapted and heavy breeds of sheep in semi-arid region of India, which is primarily reared for mutton production. Present study was carried out to evaluate the reproductive parameters and selective value and also to estimate (co)variance component for these traits in the Malpura sheep on the data spread over 35 years...

Chickens (Gallus gallus) were the first avian species selected for whole genome sequencing because of their economic value, use as a food source, livelihood security and research importance. Any living organism contains a galaxy of genes which express all the phenotypes or characters by encoding proteins and peptides, and playing regulatory roles i...

The concept of adaptability revolves around fitness describing relative ability of an individual to survive and reproduce next generation to ensure continued survival of the population and is the result of natural selection over many generations. Current trend in genetic selection has severely eroded the genetic base ignoring the diversity of the p...

The study was conducted on Sirohi kids of 3 to 6 months of age born during the year 2008-09, to assess the growth potential of kids for chevon production. Under intensive system (group 1) of feeding, 3 WT and 6 WT was 14.75 and 27.63 kg, respectively. The finishing weight was almost 2 kg more than semi-intensive group (group 2) and 6.6 kg more than...

Parasitic gastroenteritis (PGE), dominated by haemonchosis is one of the major constraints to profitable sheep production and sole reliance on chemotherapeutic measures for its control resulted in additional problem of anthelmintic resistance. Among alternate approaches, exploitation of genetic control of PGE is subject of recent interest and in th...

Hatkar, D.N., Prince, L.L.L., Sirothia, A.R. and Paswan, C. 2008. Study of genetic relatedness among meat producing Indian sheep breeds by RAPD-PCR. J. Appl. Anim. Res., 38: 129–133. To estimate the genetic variability and phylogenetic relationship among four breeds (Sonadi, Madgyal, Deccani and Nellore) of sheep adapted in the semi-arid climatic c...

(Co)variance components and genetic parameters of weight at birth (BWT), weaning (3WT), 6, 9 and 12 months of age (6WT, 9WT and 12WT, respectively) and first greasy fleece weight (GFW) of Bharat Merino sheep, maintained at Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India, were estimated by restricted maximum likelihood, fitti...

Estimates of (co)variance components were obtained for lamb growth traits in Bharat Merino sheep over a period of 17 years (1990-2006). Records of 2630 lambs from 214 sires and 949 dams were used for this analysis. Data were analyzed by restricted maximum likelihood (REML), fitting an animal model ignoring or including maternal genetic and maternal...

Bhushan B., Patra, B. N., Paswan, C., Umang, Kumar, P., Dutt, T., Das, P.J., Sharma, A. and Ahlawat, S. P. S. 2007. DNA polymorphism of BoLA-DRB3.2 gene in Tharparkar cattle (Bos indicus) by PCR-RFLP. J. Appl. Anim. Res., 32: 187–190. PCR-RFLP study of BoLA-DRB3 exon 2 was carried out in 36 Tharparkar cattle using three restriction enzymes namely H...

Characterization ofBoLA-DRB 3.2 gene was carried out in 25 crossbred cows suffering from clinical mastitis and 50 apparently healthy cows by PCR-RFLP. Digestion of 284 bp product of DRB3.2 gene with HaeIII restriction enzyme revealed five patterns out of these HaeIII-a (0.53) and -alb (0.38) being more frequent. RsaIn (0.120) pattern with highest f...

The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Five genotypes i.e. HaeIII-a, HaeIII-b, HaeIII-e, HaeIII-ab and HaeIII-ae were observed when the 284 bp PCR products were digested with H...