Chanchal Mandal

Chanchal Mandal
Khulna University | KU · Biotechnology and Genetic Engineering Discipline

PhD in Molecular and Life Science

About

36
Publications
12,605
Reads
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203
Citations
Introduction
Chanchal Mandal currently works at the Biotechnology and Genetic Engineering Discipline, Khulna University. Chanchal does research in Bioinformatics, Molecular Biology and Genetics.

Publications

Publications (36)
Article
Full-text available
Fetal alcohol spectrum disorder is a collective term representing fetal abnormalities associated with maternal alcohol consumption. Prenatal alcohol exposure and related anomalies are well characterized, but the molecular mechanism behind this phenomenon is not well characterized. In this present study, our aim is to profile important genes that re...
Article
Fetal alcohol spectrum disorder is a collective term that represents fetal abnormalities associated with maternal alcohol consumption. Prenatal alcohol exposure and related anomalies are well characterized, but the molecular mechanism behind this phenomenon is not yet understood. Few insights have been gained from genetic and epigenetic studies of...
Article
Full-text available
Ethanol is well known for its teratogenic effects during fetal development. Maternal alcohol consumption allows the developing fetus to experience the detrimental effects of alcohol exposure. Alcohol-mediated teratogenic effects can vary based on the dosage and the length of exposure. The specific mechanism of action behind this teratogenic effect...
Article
Full-text available
Ethanol is well known as a teratogenic factor that is capable of inducing a wide range of developmental abnormalities if the developing fetus is exposed to it. Duration and dose are the critical parameters of exposure that affect teratogenic variation to the developing fetus. It is suggested that ethanol interferes with epigenetic processes especia...
Article
Alcohol is a teratogenic agent that can cause a wide range of developmental disorders, and sometimes, the effects persist throughout an individual's lifetime. Researchers have shown the involvement of epigenetic mechanisms in alcohol-mediated disorders. Non-coding RNAs are one of the major sources of epigenetic modifications, especially microRNAs....
Article
Epigenetic modifications by toxic heavy metals are one of the intensively investigated fields of modern genomic research. Among a diverse group of heavy metals, lead (Pb) is an extensively distributed toxicant causing an immense number of abnormalities in the developing fetus via a wide variety of epigenetic changes. As a divalent cation, Pb can re...
Chapter
The issue of antimicrobial resistance gained current attention to scientists. The biological approaches would be the game changer to beat pathogenic microorganisms. The successful use of antibodies in different diseases raises the hope of using antibodies as antimicrobial agents. However, several obstacles—antibody production cost, allergic reactio...
Article
Full-text available
Transdifferentiation (or activation) of hepatic stellate cells (HSCs) to myofibroblasts is a key event in liver fibrosis, and activated HSCs in tumor microenvironment reportedly promote tumor progression. This study aimed to analyze the effect of an inhibitor of HSC activation, R-III (a retinol-binding protein - albumin domain III fusion protein),...
Article
Liver fibrosis is one of the leading causes for cirrhotic liver disease and the lack of therapies to treat fibrotic liver is a major concern. Liver fibrosis is mainly occurred by activation of hepatic stellate cells and some stem cell therapies had previously reported for treatment. However, due to some problems with cell-based treatment, a safe th...
Article
Full-text available
Silver nanoparticles (Ag-NPs) are among the most widely used nanoparticles that show a broad spectrum of antibacterial activity. Green synthesis of Ag-NPs from plant extract is the most popular method in vitro. In this study, Ag-NPs were biologically synthesized from the leaf extract of Moringa oleifera and the antimicrobial activity was observed....
Article
Full-text available
Interstitial fibrosis is a common feature of chronic kidney disease, and platelet-derived growth factor receptor-β (PDGFR-β)-positive mesenchymal cells are reportedly the major source of scar-producing myofibroblasts. We had previously demonstrated that albumin and its derivative R-III (a retinol-binding protein-albumin domain III fusion protein) i...
Article
In recent years, several studies have reported positive outcomes of cell-based therapies despite insufficient engraftment of transplanted cells. These findings have created a huge interest in the regenerative potential of paracrine factors released from transplanted stem or progenitor cells. Interestingly, this notion has also led scientists to que...
Article
Full-text available
Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Although, recent advances have greatly expanded the capability of yeast surface display, the protein display system is still far away from industrial application. One of the major components of a stable, efficient and successful...
Article
Full-text available
Conventional methods for covalent immobilization of proteins often result in denaturation due to chemical treatments. However, proteins immobilized at microbial cell surfaces by regular cellular processes could be bound covalently to the cell wall without being exposed to chemical treatment. Yeasts display systems provide several advantages over ba...
Article
Full-text available
Antibiotics are the secondary metabolites produced by bacteria and fungi to defend themselves from other pathogens. These secondary metabolites are being produced and used as a drug to cure different diseases. However, antibiotic resistance is a common problem that demands an urgent need to discover new antibiotics routinely. Several approaches hav...
Article
Backgrounds PU.1 is a pioneer transcription factor and a master regulator of the myeloid lineage. However, the role of PU.1 in lipopolysaccharide-dependent microglial activation has yet to be investigated. So, this study was conducted to determine the effects of PU.1 in LPS-induced activation of microglial cells. Methods We knocked out PU.1 in mur...
Article
Full-text available
Histone-modifying enzymes are key players in the field of cellular differentiation. Here, we used GSK-J4 to profile important target genes that are responsible for neural differentiation. Embryoid bodies were treated with retinoic acid (10 μM) to induce neural differentiation in the presence or absence of GSK-J4. To profile GSKJ4-target genes, we p...
Article
During the differentiation process, various epigenetic factors regulate the precise expression of important genes and control cellular fate. During this stage, the differentiating cells become vulnerable to external stimuli. Here, we used an early neural differentiation model to observe ethanol-mediated transcriptional alterations. Our objective wa...
Poster
Full-text available
Drinking during pregnancy causes harms to the developing fetus which can be persist throughout entire life. It causes mental retardation, memory problems, poor judgement, hyperactivity and other neuronal diseases. Here, we used an in vitro model of neuronal differentiation to profile ethanol-mediated alteration of gene expressions using next genera...
Poster
Full-text available
Alcohol (ethanol, EtOH) consumption during pregnancy causes a variety of prenatal and postnatal disorders collectively referred to as fetal alcohol spectrum disorders (FASDs). Recently, increasing evidence indicates that alcohol may induce epigenetic alterations, in particular aberrant DNA methylation patterns. To better understand the molecular ev...
Article
Neural differentiation involves complex changes of gene expression patterns, which are controlled by chromatin remodeling that promotes or inhibits neurogenesis and gliogenesis. To study the roles of the ubiquitously transcribed tetratricopeptide repeat on chromosome X (UTX) during neuronal differentiation, we performed gene expression analysis in...
Article
Full-text available
It is well known that consuming alcohol prior to and during pregnancy can cause harm to the developing fetus. Fetal alcohol spectrum disorder is a term commonly used to describe a range of disabilities that may arise from prenatal alcohol exposure such as fetal alcohol syndrome, partial fetal alcohol syndrome, alcohol-related neurodevelopmental dis...
Article
The objective of the present study was to investigate the changes in gene expression in the fetal brain (forebrain and hippocampus) caused by maternal binge alcohol consumption. Pregnant C57BL/6J mice were treated intragastrically with distilled phosphate-buffered saline (PBS) or ethanol (2.9 g/kg) from embryonic day (ED) 8 to 12. Microarray analys...
Poster
Full-text available
The molecular mechanism behind alcohol mediated fetal anomalies are not yet well characterized. Here, we have analyzed the gene expression pattern of human carcinoma cell-derived embryoid bodies in the absence or presence of ethanol. A cDNA microarray analysis at day 7 profiled a total of 493 differentially expressed genes in response to 50 mM etha...
Article
Toxicity of ethanol can lead to behavioral and cognitive impairments of fetus. The aim of this current study was to investigate the alteration of genes at developing fetal brain by maternal binge alcohol consumption. Genome-wide transcriptional analysis revealed a set of differentially expressed genes (20 upregulated and 21 down-regulated; 1.5-fold...
Article
Full-text available
Neural stem cells (NSCs) can be differentiated into one of three cell lineages: neurons, astrocytes or, oligodendrocytes. Some neurotoxins have the ability to deregulate this dynamic process. NSC cell fate can be altered by ethanol as reported previously. Our aim was to investigate the alteration of genes by ethanol during NSC differentiation and t...
Poster
Full-text available
Neural stem cells (NSCs) can be differentiated into one of three cell lineages: neurons, astrocytes or, oligodendrocytes. Ethanol has previously been reported to alter the cell fate of NSCs. To explore the molecular mechanism underlying this phenomenon, we performed a comparative transcriptomic analysis of mouse NSC differentiation in the presence...
Article
Full-text available
Ethanol (EtOH) exposure during embryonic development causes dysfunction of the central nervous system (CNS). Here, we examined the effects of chronic EtOH on gene expression during early stages of neuronal differentiation. Human embryonic carcinoma (NCCIT) cells were differentiated into neuronal precursors/lineages in the presence or absence of EtO...
Poster
Full-text available
Consuming alcohol prior to and during pregnancy can cause harm to the developing fetus. Here, we report that maternal binge alcohol consumption alters several important genes (Nova1, Ntng1, Gal, Neurog2, Neurod2 and Fezf2) that are involved in nervous system development in the mouse hippocampus at embryonic day 18 (ED18). Pathway analysis also reve...
Poster
Full-text available
Fetal alcohol spectrum disorder (FASD) is a set of developmental malformation in nervous system by maternal alcohol consumption during pregnancy. Objective of the present study was to investigate the alteration of gene expression in fetal brain (forebrain and hippocampus) caused by maternal binge alcohol consumption. Pregnant C57BL/6J mice were tre...
Article
Fetal alcohol spectrum disorder (FASD) is a set of developmental malformations caused by excess alcohol consumption during pregnancy. Using an in vitro system, we examined the role that chronic ethanol (EtOH) exposure plays in gene expression changes during the early stage of embryonic differentiation. We demonstrated that EtOH affected the cell mo...
Article
Dedifferentiation of more mature cell types into multipotential cells is of interest since they have potential regenerative capacity. Reversine has the ability to induce the reversion of adult cells to a multipotent state from which they can re-differentiate into other cell types when induced by appropriate culture conditions. To evaluate the dedif...
Poster
Full-text available
Ethanol exposure during embryonic development causes dysfunction of the central nervous system (CNS) and leads to fetal alcohol spectrum disorders (FASD). In this study, we examined the effects of chronic ethanol exposure on gene expression during early neuronal differentiation. NCCIT cells were differentiated into neuronal precursors/lineages by r...
Article
Full-text available
Leaf segments, internode and nodal segments of Stevia were cultured on MS medium supplemented with varying concentrations (1, 3, 5 and 7 mg L -1) of growth regulators (2,4-D, BAP and NAA) along with coconut water for observe morphogenic responses (mainly formation of callus). Ninety five percent aseptic cultures were obtained when sterilized with 0...

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Projects

Projects (2)
Project
To find the effect of nutritional status of mother to the developing fetus
Project
Effect of different media on the regenerative potential of mesenchymal stem cells and secretomes.