Chalermpong Saenjum

Chiang Mai University | CMU · Department of Pharmaceutical Sciences

Obesity, a major global health concern, is represented by an accumulation of adipose tissue and body mass, leading to a higher incidence of non-communicable diseases (NCDs). Camellia sinensis var. assamica, known as cha-miang, contains catechin and its derivatives, which have been reported to affect several health-related concerns such as anti-canc...

This study investigated the potential of microbial fermentative transforming processes in valorizing the cashew apple by-product into a low-alcohol, health-benefiting beverage. We particularly investigated the use of a non-Saccharomyces yeast, Cyberlindnera rhodanensis DK, as the main targeted microbe. At 30 °C without agitation, C. rhodanensis DK...

Bacterial pathogens have remained a major public health concern for several decades. This study investigated the antibacterial activities of Miang extracts (at non-neutral and neutral pH) against Bacillus cereus TISTR 747, Escherichia coli ATCC 22595, Salmonella enterica serovar Typhimurium TISTR 292 and Streptococcus mutans DMST 18777. The potenti...

This article reviews the use of natural resources in analytical chemistry throughout history. Plant extracts were employed as indicators in chemistry for identifying the acidity or alkalinity of liquids as early as the 1650s. Later, as the industrial revolution altered people’s lives, synthetic chemicals were used instead. Modern techniques of anal...

This study focused on isolating tannin-tolerant yeasts from Miang, a fermented tea leaf product collected from northern Laos PDR, and investigating related food applications. From 43 Miang samples, six yeast isolates capable of ethanol production were obtained, with five isolates showing growth on YPD agar containing 4% (w/v) tannic acid. Molecular...

In this work, we prepared a series of magnetic carbonaceous materials (CB200F, CB400F, CB600F, CB800F, and CB1000) from waste bamboo (RB) for the removal of cesium ions from water. Physicochemical properties such as pHpzc, specific surface area, and surface functional groups of the prepared adsorbents were evaluated and their cesium ion adsorption...

A sustainable procedure offering green, simple, and rapid analysis was developed to determine benzalkonium chloride (BKC) in pharmaceutical preparations. The determination using smartphones was based on the ion pair colorimetric reaction with bromothymol blue (BTB), which produces a yellow color. The intensity of the product color, which is proport...

Citation: Kham, N.N.N.; Phovisay, S.; Unban, K.; Kanpiengjai, A.; Saenjum, C.; Lumyong, S.; Shetty, K.; Khanongnuch, C. A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production. J. Fungi 2024, 10, 243. https://doi. Abstract: This study aims to utilize the microbial re...

Carica papaya L. leaves, traditionally utilized in dietary supplements and pharmaceuticals, exhibit a broad spectrum of potentially therapeutic properties, including anti-inflammatory, antimalarial, and wound healing properties. This study examined the acute and chronic toxicity of 10% ethanolic-extracted C. papaya leaf in Sprague Dawley rats. The...

Fine Na-type zeolite grains (ZE) were prepared by dry milling treatment (D-ZE) or wet milling treatment (W-ZE). Then, the prepared samples were characterized. The effective parameters of adsorption capacities including the initial concentration, adsorption temperature, pH, and contact time for lead-ion adsorption were determined. The results reveal...

Objective Plants in the Annonaceae family are known for having abundant biologically active secondary metabolites. They have been used in alternative drugs for various diseases in several countries, for instance, the bark of Cananga odorata (Lam.) Hook and Thomson is used for Ophthalmic inflammation and wound healing in Malaysia. Extracts from the...

Mango (Mangifera indica L.) is one of the most economically important fruits in Thailand. Mango has been used as a traditional medicine because it possesses many biological activities, such as antioxidant properties, anti-inflammatory properties, microorganism-growth inhibition, etc. Among its natural pharmacologically active compounds, mangiferin...

This research evaluated the role and feasibility of the granular nanocellulose particles (GNC) from sugarcane bagasse obtained from enzymatic hydrolysis in reducing lipid digestibility and permeability in an in vitro simulated gastrointestinal (GI) system. GNC concentration (0.02%, w/v) had significantly affected the released free fatty acids (FFA)...

This research evaluated the role and feasibility of the granular nanocellulose particles (GNC) from sugarcane bagasse obtained from enzymatic hydrolysis in reducing lipid digestibility and permeability in the in vitro simulated gastrointestinal (GI) system. GNC concentration (0.02%, w/v) had significantly affected the released free fatty acid (FFA)...

Second-generation lactic acid production requires the development of sustainable and economically feasible processes and renewable lignocellulose biomass as a starting raw material. Weizmannia coagulans MA42 was isolated from a soil sample in Chiang Mai province, Thailand and showed the highest production of L-lactic acid and lignocellulolytic enzy...

The efficient pretreatment of lignocellulosic biomass is essential for cellulose valorization. This work investigated the fractionation efficiency of the pretreatment process based on natural acidic deep eutectic solvent (NADES) for isolating cellulose-rich materials (CRM) from coffee parchment (CP). The results showed that NADES, composed of choli...

A colloidal silicate granulated nickel–aluminum–zirconium (CSG-NAZ) was prepared, and the chromium(VI) (Cr(VI)) ions recovery capacity was evaluated using a sodium sulfate solution in a column experiment. The amount adsorbed and breakthrough time were enhanced by decreasing the flow rate (flow rate is in the order of 3.0 > 2.0 > 0.5 mL). The breakt...

Northern Indochina is a region with abundant and diverse natural resources, including ethnomedicinal plants that have been traditionally used to alleviate cancer symptoms. However, the literature reviews regarding the scientific and clinical validation of the active ingredients and signaling mechanisms for cancer therapeutic application are still l...

In Thailand and worldwide, smartphone addiction among university students is a growing concern. This study aims to investigate behaviors of smartphone use, the prevalence of smartphone addiction, the duration of smartphone use, and their associated factors among pharmacy students at a university in northern Thailand. This cross-sectional study was...

In this study, waste biomass adsorbents produced from mangosteen shells (MGS) were prepared (denoted as MGS500 and MGS1000). The physical and chemical characteristics, such as scanning electron microscopy, thermogravimetric-differential thermal analysis, specific surface area, pore volumes, surface functional groups, and point of zero charge of the...

This study aims to investigate the antioxidant and anti-cancer activities of Clerodendrum chinense leaf ethanolic extract. The phenylethanoid glycoside-enriched extract, namely verbascoside and isoverbascoside, was determined in the ethanolic C. chinense leaf extract using the validated HPLC method. The ethanolic extract showed DPPH and ABTS free r...

Background Elsholtzia is a genus in the family Lamiaceae, and some species in this genus are commonly used for food and in ethnomedicinal formulations by some ethnic groups of China and Thailand. Despite their apparent utility, few studies have been conducted to evaluate their potential as sources of medicinally active agents. Purpose We aimed to...

An investigation was made to use some vegetable oils, namely, extra virgin coconut oil, tea seed oil, soybean oil, and extra light olive oil, as alternative solvents for the simple green colorimetric liquid-liquid partitioning extraction determination of diphenhydramine hydrochloride (DPHH). The ion pair of DPHH and bromocresol green (BCG) in an aq...

This research demonstrated an excellent potential approach for utilizing Miang fermentation broth (MF-broth), a liquid residual byproduct from the Miang fermentation process as a health-targeted beverage. One hundred and twenty yeast strains isolated from Miang samples were screened for their potential to ferment MF-broth and four isolates, P2, P3,...

Inflammatory bowel disease (IBD) has become a global concern. Proanthocyanidin-rich red rice extract (PRRE) has been shown to suppress the inflammatory response in cellular cultures. However, the anti-colitis effect of PRRE has never been investigated in animals. This study aimed to examine the protective effect of the PRRE against dextran sulfate...

The concept that aging is a disease and the rise in the elderly population all over the world have increased the need for drugs to intervene the aging and age-induced impairments. Bioactives derived from plants have shown the possibility to be utilized as potential natural remedies against many age-related complications, such as cancer, cardiovascu...

A green and sustainable downscaled procedure was developed to perform smartphone-based colorimetric determination of fluoroquinolones in extemporaneous syrup formulations. The procedure functioned by complexing fluoroquinolones with metal ions, the most suited of which is a ferric ion, to produce a colored product in a 96-well plate combined with s...

This study reviews the preparation of adsorbents, specifically wheat bran, from waste biomass for the adsorption/recovery of Pt(IV) ions from aqueous phase. Herein, the physicochemical characteristics of virgin wheat bran (WB) and WB calcined at 500 °C and 1000 °C (WB500 and WB1000, respectively) were evaluated. Elemental distribution and binding e...

Chlorhexidine gluconate (CHG) is a cationic disinfectant. The positive charge of CHG molecules binds to phospholipid's negative charge in bacterial cell walls, causing membrane disruption. The in vitro kinetic physical, chemical and biological incompatibilities of nine lubricating gels with 1% w/v CHG were investigated. Five containing anionic thic...

A sustainable downscaled procedure using smartphone-based colorimetric determination of manganese (Mn(II)) was developed. This novel Mn(II) determination procedure is proposed using a simple, available microwell-plate platform and a smartphone as a detector. This approach is based on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by periodat...

Caesalpinia sappan L. heartwood was collected from Mae Chaem District, Chiang Mai Province, Thailand. Crude extracts were prepared by Soxhlet’s extraction using 50, 60, and 70% of ethanol (EtOH) at 50, 60, and 70 °C, and the brazilin content was measured using reversed-phase high performance liquid chromatography (RP-HPLC). The antibacterial activi...

“People-Forest-Miang” communities are villages located in the cultivated area of Camellia sinensis var. assamica, or Cha Miang, in northern Thailand. Cha Miang forests are a form of agriculture relying on forest-rich bioresources. This study focuses on a survey of the diversity of medicinal plants used by “People-Forest-Miang” communities in Mae Ka...

Coal fly ash (FA) was treated by hydrothermal activation with sodium hydroxide solution at different concentrations to optimize the conversion method. Zeolite of the sodium type is prepared from coal FA by 1, 1.5, and 3 mol/L sodium hydroxide solutions (ZE1, ZE1.5, and ZE3). These adsorbents’ morphology, crystal structure, scanning electron microsc...

To increase the adsorption capability of Hg2+ from aqueous media, we prepared sodium-type fine zeolite grains with various particle sizes (denoted as ZE1, ZE2 and ZE3). The particle sizes of ZE1, ZE2 and ZE3 were 16.363 ± 0.365, 1.454 ± 0.357 and 0.607 ± 0.377 μm, respectively. Moreover, the CEC, specific surface area and pore volume were in the or...

We combined a nickel–aluminum–zirconium complex hydroxide (NAZ) with colloidal silica as a binder to prepare a granulated agent for adsorbing heavy metals from aqueous media. Three samples with different particle diameters were prepared to evaluate the effects on the properties: small (NAZ-S), medium (NAZ-M), and large (NAZ-L). We confirmed the gra...

The COVID-19 pandemic has forced analytical chemistry educators in Thailand to change methods of teaching and learning to new normal ones. Higher education has faced additional challenges because of a lack of hands-on experiments and an increasing number of students in foundation chemistry courses being hindered from practicing skills. This work ai...

This study aimed to study the biotransformation of indigenous northern Thai purple rice using β-glucosidase-producing Lactobacillus (BGPL) to increase the content of bioactive anthocyanin for colorectal chemoprevention and immunization. BGPL, namely, Lactobacillus FR 332, was first isolated from Thai fermented foods. Indigenous northern Thai purple...

Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that Perilla frutescens seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hex-ane fraction (PLH) in osteoporosis has not yet been in...

Background. Cissus quadrangularis Linn. (CQ) is a medicinal plant with good evidence for the treatment of hemorrhoids, listed in the Thai National List of Herbal Products in the oral dosage form. Acmella paniculata (Wall ex. DC.) R. K. Jansen. (AP) is a medicinal plant with a local anesthetic effect. Objective. To investigate the potential of recta...

To minimize chemical waste and protect the environment, our team has used green analysis with natural reagents. In this work, we designed a natural-reagent assay kit for iron determination and implemented it in chemistry education in Thailand. The iron assay method was adapted from Thai local wisdom of testing water quality using guava leaves. The...

High performance liquid chromatography (HPLC) for catechins and related compounds in Miang (traditional Lanna fermented tea leaf) was developed to overcome the matrices during the fermentation process. We investigated a variety of columns and elution conditions to determine seven catechins, namely (+)-catechin, (-)-gallocatechin, (-)-epigallocatech...

A high-sensitivity green analytical methodology was developed for the spectrophotometric determination of tetracycline (TC), employing simultaneous injection effective mixing analysis (SIEMA). This is based on the chelation between tetracycline and zirconium, resulting in a yellow-colored product which exhibits maximum absorption at 420 nm. Under o...

In this paper, a novel antioxidant analysis is proposed using a simple minimized device based on moving drops as solution handling and a smartphone as a detector. This approach is based on the colorimetric determination of the scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), expressed as the half-maximal inhibitory concent...

Five glutinous purple rice cultivars and non-glutinous purple rice cultivated in different altitudes in the north of Thailand were collected. The samples were extracted using ethanol and determined for anthocyanins using HPLC. The total phenolic content (TPC), total flavonoid content (TFC), and the antioxidant, anti-inflammatory, and antimicrobial...

Guanidinyl tryptophan derivatives TGN1, TGN2, TGN3, and TGN4 were synthesized, and these compounds were shown to possess in vitro inhibitory activity for amyloid aggregation in a previous study. Nevertheless, the influence of the TGN series of compounds on the binding and permeation behaviors of an Aβ monomer to the cell membranes was not elucidate...

The current study investigated the inhibiting effect on reactive oxygen species (ROS), reactive nitrogen species (RNS), and matrix metalloproteinase-1 (MMP-1) production in a cell-based study of standardized rosmarinic acid enriched extract (SRAEE) prepared from Thunbergia laurifolia leaves. HPLC chromatogram revealed that rosmarinic acid is a majo...

The characteristics of acid-activated raw bentonite (RB) activated with binary acid solutions sulfuric acid + nitric acid, nitric acid + phosphoric acid, and phosphoric acid + sulfuric acid, at a concentration of 5 mol/L (denoted as 5-SN, 5-NP, and 5-PS), were evaluated. Moreover, its application for improving the electrical conductivity in tap wat...

A modern chemical sensor system (M-CSS) was developed for the cost-effective chemical analysis of Thai precision and sustainable agriculture (TPSA), which is suitable in rural Thailand and elsewhere. The aim of this study was to achieve precision and sustainable agriculture (P-SA). The M-CSS functions according to the International Union of Pure an...

The study investigated the impact of the fermentation process on the phenolic contents and antioxidant and anti-inflammatory activities in extracts of Miang, an ethnic fermented tea product of northern Thailand. The acetone (80%) extraction of Miang samples fermented by a non-filamentous fungi-based process (NFP) and filamentous fungi-based process...

Miang or fermented tea leaf has been a part of the Mae Kampong community, one of northern Thailand’s famous tourist attractions, for a long time. Although the demand and popularity of Miang are decreasing, Miang is still important to the community lifestyle in various ways. Thus, this study aimed to investigate Miang consumption behavior, lifestyle...

Raw and calcined basil seeds (BS and BS1000, respectively) were evaluated for their ability to remove herbicides such as paraquat and diquat. The physicochemical properties of BS and BS1000 were determined and the effects of contact time and initial concentration on paraquat and diquat adsorption were assessed. After calcination treatment, the numb...

In this study, we investigated the antioxidant and anti-inflammatory phytochemicals and paramagnetic species in dragon fruit using high-performance liquid chromatography (HPLC) and electron paramagnetic resonance (EPR). HPLC analysis demonstrated that dragon fruit is enriched with bioactive phytochemicals, with significant variations between each p...

The feasibility of using potassium-type zeolite (K-type zeolite) prepared from coal fly ash (CFA) for the removal of Hg2+ from aqueous media and the adsorption/desorption capabilities of various potassium-type zeolites were assessed in this study. Potassium-type zeolite samples were synthesized by hydrothermal treatment of CFA at different interval...

The microbial community in ethnic fermented tea, “Miang” prepared from Camellia sinensis var. assamica in northern Thailand was investigated during the 10 days of a filamentous fungi growth-based process (FFP) using both culture-dependent and culture-independent techniques. During the aerobic phase (days 1-3), filamentous fungi were visually observ...

To evaluate the feasibility of nickel–aluminum (the Ni2+:Al3+ molar ratios of 1.0:1.0 and 1.0:2.0 are denoted as NA11 and NA12, respectively) and nickel–aluminum–zirconium type (the Ni2+:Al3+:Zr4+ molar ratios of 0.9:1.0:0.09 and 0.9:2.0:0.09 are denoted as NAZ1 and NAZ2, respectively) hydroxides for Cr(VI) removal from aqueous media, the adsorptio...

The aim of this study is to determine antioxidant and anti-inflammatory activities relating to the antiosteoporosis effects of various perilla seed meal (PSM) fractions. The remaining waste of perilla seed obtained from cold oil compression was extracted with 70% ethanol and sequentially fractionated according to solvent polarity with hexane, dichl...

This study aimed to develop, characterize, and investigate the cosmeceutical activities and irritation properties of Punica granatum peel extract (PE) loaded nanofibers. Nanofibers from polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP K90) were prepared using an electrospinning technique and characterized by scanning electron microscope, Fouri...

This study aimed to investigate the characteristics of acid-activated bentonite by focusing on its capability of improving the quality of tap water used during wire electrical discharge machining. Raw bentonite (RB) was activated using sulfuric acid, nitric acid, and phosphoric acid solutions with concentrations of 1, 5, and 10 mol/L, respectively....