Chaitanya Tellapragada

Karolinska Institutet | KI · Department of Laboratory Medicine

Objectives: We evaluated performance of an isothermal microcalorimetry (IMC) method for determining the minimal inhibitory concentrations (MICs) among extensively drug resistant (XDR) Gram-negative bacilli. Methods: A collection of 320 clinical isolates (n=80 of each) of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acineto...

The study was undertaken to evaluate the performance of Unyvero Hospitalized Pneumonia Panel (HPN) Application, a multiplex PCR based method for the detection of bacterial pathogens from lower respiratory tract (LRT) samples, obtained from COVID-19 patients with suspected secondary hospital-acquired pneumonia. Residual LRT samples obtained from cri...

Objectives: The worldwide emergence of antibiotic resistance calls for effective exploitation of existing antibiotics. Antibiotic combinations with different modes of action can synergize for successful treatment. In the present study, we used microcalorimetry screening to identify synergistic combination treatments against clinical MDR isolates. T...

Purpose of review: Gram-negative bloodstream infections (GNBSI) are common and carry considerable mortality. Treatment is complicated by increasing antimicrobial resistance, posing a challenge for timely appropriate antibiotics and limiting the choices of effective definitive therapy. The present review aims to summarize recent studies addressing...

Objective The present study was aimed at elucidating the epidemiology of sepsis, with a special emphasis on identifying the common bacterial aetiology, proportion of infections caused by multi-drug resistant (MDR) bacteria, and risk factors associated with 28-day mortality at a university hospital in South India. Methods A prospective study was un...

Nucleoside analogues have been in clinical use since 1960s and they are still used as the first therapeutic option for several cancers and viral infections, due to their high therapeutic efficacy. However, their wide clinical acceptance has been limited due to their high toxicity and severe side effects to patients. Herein, we report on a nanocarri...

Multiple episodes of Enterococcus faecalis bacteremia (EfsB) may indicate a relapse and be due to an undiagnosed infective endocarditis (IE). The aims were to study the clinical presentation of patients with EfsB with focus on the risk of recurrent infection and IE, identify potential improvements of the management, and to investigate whether E. fa...

Objectives : To evaluate the activity of meropenem-amikacin and meropenem-colistin combinations with checkerboard broth microdilution (CKBM) compared to isothermal microcalorimetry (ITMC) assays against a multicentric collection of multidrug-resistant Gram-negative (MDR-GN) clinical isolates, to compare the Fractional inhibitory concentration index...

Melioidosis is a seasonal infectious disease in tropical and subtropical areas caused by the soil bacterium Burkholderia pseudomallei. In many parts of the world, including South West India, most cases of human infections are reported during times of heavy rainfall, but the underlying causes of this phenomenon are not fully understood. India is amo...

We investigated the concordance between the Unyvero Hospitalized Pneumonia (HPN) application and quantitative culture for detection of bacterial pathogens from serial lower respiratory tract (LRT) specimens collected from the same subject. Comparison of results from HPN application and culture was evaluated using 69 LRT samples from 27 subjects, us...

The recent pandemic, Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has devastated humanity and is continuing to threaten us. Due to the high transmissibility of this pathogen, researchers are still trying to cope with the treatment and prevention of this disease. Few of them were successf...

The study was undertaken to evaluate the performance of Unyvero Hospitalized Pneumonia (HPN) panel application, a multiplex PCR-based method for the detection of bacterial pathogens from lower respiratory tract (LRT) samples, obtained from COVID-19 patients with suspected secondary hospital-acquired pneumonia. Residual LRT samples obtained from cri...

The vast majority of human immunodeficiency virus (HIV) infections across the world are transmitted sexually, so understanding the correlates of protection at the genital mucosa level is very important. The genital tract mucosa is a primary site of HIV replication, and discerning the nature of HIV-specific immune responses in this compartment is cr...

Clostridioides difficile infection represents a growing clinical challenge. The new compound omadacycline is a potential treatment alternative, as many antibiotics have limited activity or are rarely used due to costs and side effects. The activity of omadacycline and five comparators was assessed with agar dilution on a 2015-to-2018 collection of...

Background Predicting spontaneous preterm birth (SPTB) during mid-trimester would be very useful. We used a multimodality screening approach mainly focusing on urogenital infections among unselected obstetric population between 18 and 24 weeks in a tertiary center. Method Diagnosis of lower genital tract infection (LGTI) was attempted among 228 pr...

Background Screening of lower genital tract infection (LGTI) in mid-trimester prevents preterm birth (PTB). There is no consensus on best screening methods for LGTI-symptoms, speculum findings, a point of care tests or high vaginal swab microscopy. Aim To know diagnostic accuracy of screening of lower genital tract infection (LGTI) in mid-trimeste...

Background: Melioidosis is gaining recognition as an emerging infectious disease with diverse clinical manifestations and high-case fatality rates worldwide. However, the molecular epidemiology of the disease outside the endemic regions such as northeast part of Thailand and northern Australia remains unclear. Methodology/principal findings: Cli...

Aims In this present study, the utility of a newly developed Loop mediated isothermal amplification (LAMP) and real‐time PCR assays designed to amplify the virB gene region of B. melitensis were evaluated from human clinical specimens. Methods and Results 54 culture confirmed cases of brucellosis and 54 culture negative but clinically suspected ca...

Background Melioidosis is gaining recognition as an emerging infectious disease with diverse clinical manifestations and high-case fatality rates,worldwide. However, the molecular epidemiology of the disease outside the endemic regions such as,Thailand and Northern Australia remains unclear. Methods Clinical data and B. pseudomallei (Bps) isolates...

Background: Radical cure of Plasmodium vivax malaria requires treatment with a blood schizonticide and a hypnozoitocide (primaquine) to eradicate the dormant liver stages. There has been uncertainty about the operational effectiveness and optimum dosing of the currently recommended 14-day primaquine (PQ) course. Methods: A two centre, randomized...

The aim of our study was to examine and compare the clinical presentations, complications, laboratory findings, treatment and outcome of patients with acute, subacute and chronic forms of brucellosis in a tertiary care setting. This hospital-based observational study was undertaken between April 2015 and March 2017. Patients diagnosed with brucello...

Two commercially available alginate impression materials were incorporated with varying concentrations of zinc oxide nanoparticles (ZnO-NP) or copper oxide nanoparticles (CuO-NP) and their properties such as antimicrobial activity, gel strength, permanent deformation, gelation time and flow were measured. The results showed that alginates incorpora...

Background: Melioidosis is endemic in northern Australia, South and South East Asia. Infection usually follows percutaneous inoculation or inhalation of the causative bacterium, Burkholderia pseudomallei, which is present in soil and surface water in the endemic region. Our aim was to study the clinical, radiological and laboratory presentations of...

Objectives: The main objective of the present in vitro study is to evaluate the antimicrobial activity and properties of irreversible hydrocolloid impression material incorporated with silver nanoparticles of varying size at different concentrations. Methods: Silver nanoparticles of 80-100, 50-80, 30-50 and 10-20nm size were added to irreversibl...

Melioidosis is a fatal infection caused by the soil saprophyte Burkholderia pseudomallei. Early diagnosis and befitting medical management can significantly influence the clinical outcomes among patients with melioidosis. Witnessing an annual increment in the number of melioidosis cases, over the past few years, mainly from the developing tropical...

Melioidosis has recently gained importance as an emerging disease in India. Recurrent melioidosis has been reported from different parts of the world and can be due to relapse or reinfection. Distinction between relapse and reinfection is important for epidemiology, investigation and management. Here, we present the data regarding rate of recurrenc...

There is a slow but steady rise in the case detection rates of melioidosis from various parts of the Indian sub-continent in the past two decades. However, the epidemiology of the disease in India and the surrounding South Asian countries remains far from well elucidated. Multi-locus sequence typing (MLST) is a useful epidemiological tool to study...

Therapeutic options for the treatment of melioidosis caused by Burkholderia pseudomallei are limited due to theinherent resistance conferred by this pathogen to various groups of antibiotics. Witnessing an increase in the numberof microbiological culture‑confirmed cases of melioidosis at our settings in the past few years, we undertook this study t...

The present study was undertaken to evaluate the efficacy of clinical and microbiological investigations available in limited resource settings for an effective diagnosis of vaginal infections/abnormal vaginal microbiota among pregnant women. As an outcome of the study we intended to find the association of various vaginal infections during pregnan...

Objectives: The present study was undertaken to study the maternal risk factors for preterm birth (PTB) and low birth weight (LBW) with a special emphasis on assessing the proportions of maternal genitourinary and periodontal infections among Indian women and their association with adverse pregnancy outcomes. Methods: A hospital-based prospective...

Background: Although melioidosis, is an important disease in many Southeast Asian countries and Australia, there is limited data on its prevalence and disease burden in India. However, an increase in case reports of melioidosis in recent years indicates its endemicity in India. Aims and methods: A population-based cross-sectional seroprevalence...

STROBE checklist. (DOC)

Statement of problem: Conventional spray and the immersion disinfection of irreversible hydrocolloid impression materials may lead to dimensional changes. Purpose: The purpose of this in vitro study was to investigate the antimicrobial activity and properties of irreversible hydrocolloid impression materials incorporated with silver nanoparticle...

Here, we report the draft genome sequence of Burkholderia pseudomallei CM_Manipal, the causative agent of melioidosis isolated from a diabetic patient in Manipal, southern India. The draft genome consists of 107 contigs and is 7,209,157 bp long. A total of 5,600 coding sequences (CDSs), 60 tRNAs, 12 rRNAs, and one noncoding RNA (ncRNA) were predict...

Candida spp. have emerged as successful pathogens in both invasive and mucosal infections. Varied virulence factors and growing resistance to antifungal agents have contributed to their pathogenicity. We studied diagnostic accuracy of HiCrome Candida Differential Agar and Vitek 2 Compact system for identification of Candida spp. in comparison with...

Early diagnosis and appropriate treatment play an important role in combating the mortality and morbidity caused by Tuberculosis and a rapid, reliable microscopy that can detect acid fast bacilli can be a very useful diagnostic modality. We have evaluated the performance of Light Emitting Diode Fluorescence Microscopy as an alternative to the exist...

Background Maternal lower genital tract during pregnancy is a complex niche of microbes that normally inhabit or cause infections in few instances. Association of various microbial flora and adverse pregnancy outcomes is being increasingly explored. The study was aimed to determine the prevalence of lower genital tract infections (LGTI)among pregna...

In view of recent understanding of the association of periodontal infections and adverse pregnancy outcomes, the present investigation was undertaken to study the periodontal infections among 390 asymptomatic pregnant women and to find an association of bacterial etiologies with the disease. Prevalence of gingivitis was 38% and clinical periodontit...

Early diagnosis and appropriate treatment play an important role in combating the mortality and morbidity caused by Tuberculosis and a rapid, reliable microscopy that can detect acid fast bacilli can be a very useful diagnostic modality. We have evaluated the performance of Light Emitting Diode Fluorescence Microscopy as an alternative to the exist...

To analyze the epidemiology and laboratory characteristics of Staphylococcus aureus bacteremia (SAB) in an Indian tertiary care hospital. We collected clinical, epidemiological, and laboratory data of all cases of SAB during August 2010 through July 2011. All isolates were tested for the Panton-Valentine leukocidin (PVL) gene. Eighteen percent of a...

Background: Forty adult volunteers were recruited to a study of Salmonella Typhi (S. Typhi) infection in human volunteers. To determine the host response to infection, participants received a S.Typhi challenge dose of 1-5 × 103 or 1-5 × 104 colony forming units (CFU). The antibody secreting cell (ASC) response in infected individuals was studied to...