Cesar Ordóñez

• University of Leon

The role of nursing students as future health promoters of healthy behaviors is influenced by their attitude towards smoking. A cross-sectional study using a self-administered survey was performed to analyze smoking prevalence among undergraduate the nursing students who attend nursing faculties in 2 European countries (Spain and Portugal) during a...

Neglected Tropical Diseases (NTD) represent a serious threat to humans, especially for those living in poor or developing countries. Almost one-sixth of the world population is at risk of suffering from these diseases and many thousands die because of NTDs, to which we should add the sanitary, labor and social issues that hinder the economic develo...

Leishmaniasis is a group of zoonotic diseases caused by a trypanosomatid parasite mostly in impoverished populations of low-income countries. In their different forms, leishmaniasis is prevalent in more than 98 countries all over the world and approximately 360-million people are at risk. Since no vaccine is currently available to prevent any form...

To analyse changes in prevalence, knowledge, beliefs and attitudes relating to smoking among undergraduate nursing and physiotherapy students over a 10-year period. Few studies have been performed to describe changes in the use of tobacco and associated characteristics. This study was a sequential cross-sectional study. A self-administered survey w...

Background As future health educators, nursing and physiotherapy students will play an essential role in the prevention of smoking.AimTo determine the prevalence of smoking among students and to analyse their knowledge of and attitudes towards smoking.Method This was a descriptive cross-sectional study using an anonymous questionnaire and conducted...

We performed a study in the city of Leon and its outskirts during the months of February and March 2010 using personal interviews as a survey method. These surveys have been developed using the "Sphinx Survey” tool, and are composed of 60 items, taking into account different classificatory variables (sex, age, occupation, education, health status)....

Forty captive Mallards (Anas platyrhynchos), of both sexes, were separated into five groups and dosed with lead shot via oral intubation; one group was used as a control. Lead dosage differed in terms of shot number and size, as well as administration time. One hundred and thirty-five wild mallards were trapped between 1998 and 2001 in the Boada an...

Cryptosporidium parvum, one of the most important causative organisms of human diarrheas during childhood, contains a monomeric DNA-topoisomerase IB (CpTopIB) in chromosome 7. Heterologous expression of CpTopIB gene in a budding yeast strain lacking this activity proves that the cryptosporidial enzyme is functional in vivo. The enzymatic activity i...

Sodium butyrate is a sodium salt of a volatile short-chain fatty acid (butyric acid) used to prevent Salmonella Enteritidis infection in birds. Three groups of fifty 1-d-old broilers each were fed the following diets: T0 = standard broiler diet (control); T1 = standard broiler diet supplemented with 0.92 g/kg of an additive with free sodium butyrat...

Background: Intervention in the prevention and control of the smoking habit among health sciences students in order to improve the beliefs and attitudes of future professionals concerning this serious public health problem. The objective of this study is to compare the validity of three different methods for calculating the prevalence of smokers. M...

Intervention in the prevention and control of the smoking habit among health sciences students in order to improve the beliefs and attitudes of future professionals concerning this serious public health problem. The objective of this study is to compare the validity of three different methods for calculating the prevalence of smokers. First-year st...

Cytotoxicity of two insect growth regulators, diflubenzuron, a benzoylphenylurea derivative that inhibits the synthesis of new chitin in target organisms, and pyriproxyfen, an insect juvenile hormone analogue, were tested on CHO-K1 cultures, using the neutral red incorporation assay. Both compounds displayed cytotoxic effects that rise with time ex...

1. The basal cytotoxic effect of the organochlorine pesticides hexachlorocyclohexane and lindane on CHO-K1 cultures was assessed at fractions of their lethal doses as determined by the neutral red incorporation (NRI) assay (NRI(6.25), NRI(12.5) and NRI(25)). The sulphur-redox cycle enzymes glutathione peroxidase, glutathione reductase and glutathio...

The effects of cadmium (Cd(2+)), mercury (Hg(2+)), lead (Pb(2+)), copper (Cu(2+)) and nickel (Ni(2+)) on the glutathione (GSH)-redox cycle were assessed in CHO-K1 by the neutral red uptake inhibition (NR) assay (NR(6.25), NR(12.5) and NR(25)). Mercury proved to be the most and lead the least toxic of the metals tested. The effects on GSH content an...

Methionine adenosyltransferase (MAT) catalyzes the synthesis of s-adenosylmethionine (AdoMet), a metabolite that plays an important role in a variety of cellular functions, such as methylation, sulfuration, and polyamine synthesis. In this study, genomic DNA from the protozoan parasite Leishmania infantumwas cloned and characterized. L. infantum MA...

Se ha determinado la citotoxicidad del fungicida ditiocarbámico mancozeb, en cultivos celulares de ovario de hámster (CHO-K1), usando los bioensayos estandarizados de incorporación de rojo neutro (RN) y del contenido total de proteínas (PT). Las dos técnicas mostraron ser comparables en la determinación del efecto citotóxico, mostrando valores de R...

Residue analysis of seven organophosphorous insecticides in fruit by GC/NPD and confirmation by mass spectrometry. The presence of seven organophosphate residues has been studied in apples and pears acquired from the market in León (Spain). An extraction system for aqueous matrixes and a multiresidue analysis based on gas chromatography (GC) with n...

The cytotoxicity of the dithiocarbamate fungicide mancozeb was assessed on hamster ovary cell culture (CHO-K1), using the neutral red incorporation (RN) and total protein content (PT) assays as endpoints. Both techniques were reliable in evaluating the cytotoxic parameters of mancozeb. The RN50 value calculated by probit/log curves was lower than 1...

The presence of seven organophosphate residues has been studied in apples and pears acquired from the market in León (Spain). An extraction system for aqueous matrixes and a multiresidue analysis based on gas chromatography (GC) with nitrogen phosphorous detection (NPD) have been used. Positive samples were confirmed by gas chromatography coupled t...

The effect of the cyclodiene organochlorine pesticides aldrin, dieldrin and endosulfan was assessed on CHO-K1 cultures at fractions of their lethal doses, determined by the neutral red (NRI) incorporation assay (NRI6.25, NRI12.5 and NRI25). Glutathione peroxidase, reductase and S-transferase, and total and oxidised glutathione were evaluated along...

A method using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS) has been developed for the characterization and determination of pyridoquinoline derivatives 4,6-bis(dimethylaminoethylamino)-2,8,10-trimethylpyrido[3,2-g]quinoline, 4,6-bis(dimethylaminoethoxy)-2,8,10-trimethylpyrido[3,2-g]quinoline and 4,6...

Wistar rats were subjected to a 90 days physical aerobic training plus repeated doses of salbutamol, a β2-adrenergic agosnist. Rats were treated with salbutamol at two different doses; therapeutic (16 μg/kg body weight, twice a day), and doping (3 mg/kg weight, twice a day), and they were training with an aerobic protocol throughout the experiment...

On Dec 15, 2000 this sequence version replaced gi:11037717.

The effect of herbicide paraquat has been assessed on CHO-K1 cultures at different concentrations. Glutathione peroxidase, reductase and S-transferase, as well as total and oxidized glutathione, were evaluated along the standard growth curve of the cultures. Paraquat was then administered during mid-log phase at concentrations that produced a calcu...

Treatment of experimental animals subjected to 90 days physical training programme plus repeated doses of salbutamol, a beta-adrenergic agonist, administered under two different regimes: therapeutic (16 microg/kg body weight, twice a day) and doping (3 mg/kg body weight, twice a day), caused a marked increase in size of skeletal (soleus, gastrocnem...

On Oct 27, 2000 this sequence version replaced gi:5106756.

Methionine adenosyltransferase (MAT), S -adenosylmethionine (AdoMet), and S -adenosylhomocysteine (AdoHcy), have been analysed at different time-points during the growth curve of Leishmania infantum. MAT activity and AdoMet content peaked in the lag and early log phases, whereas higher levels of AdoHcy were found in stationary phase cells. MAT acti...

An acute treatment of mice with clenbuterol, a beta-adrenergic agonist, produced a marked increase of polyamines levels in heart, particularly during the early phase of administration of the drug. A single dose of 1.5 mg/kg caused as much as a 10 fold induction in activity of ornithine decarboxylase (ODC) and 3 to 4 fold increase in levels of putre...

To evaluate the cytotoxicity of seven metals, two lines of fish cell culture, BF-2 and RTG-2 were used. The first cell line (BF-2). derived from the caudal fin portion of the trunk of bluegill sunfish and the second fish cell line, (RTG-2), derived from the gonadal tissues of the rainbow trout fish, both representing continental water organisms. Tw...

The induction of hypertrophy of cardiac and skeletal muscles has been studied after treatment with two different salbutamol dosages, therapeutic and doping. Treatment of rats subjected to a physical training schedule with repeated doses (16 μg kg−1 per day or 3 mg kg−1 per day) of salbutamol, a specific β-adrenergic agonist, induced a marked increa...

The use of β-agonists as growth-promoting agents in cattle could lead to toxic side-effects in man. One such effect is the accumulation of polyamines which seem to be implicated in muscle and heart hypertrophy. We have studied the induction of cardiac hypertrophy after treatment with clenbuterol and the role of polyamines in this effect. Treatment...

The cytotoxicity of twelve organochlorine insecticides and seven metals has bene evaluated by four different in vitro alternative methods using the established CHO-K1 cell line as a mammalian model. The assessed techniques represent distant endpoints, neutral red (NR) assay, methyl tetrazolium test (MTT) assay, total cell protein content assay and...