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Cedric Jacqueline

Cedric Jacqueline
University of Nantes, IRS2 Nantes - Biotech · UPRES EA 3826 Thérapeutiques Cliniques et Expérimentales des Infections

PhD

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149
Publications
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Publications

Publications (149)
Article
Full-text available
Rationale: Brain injury induces systemic immunosuppression increasing the risk of viral reactivations and altering neurological recovery. Objectives: To determine if systemic immune alterations and lung replication of Herpesviridae are associated and can help predict outcomes after brain injury. Methods: We collected peripheral blood mononucle...
Article
Full-text available
At the early stages of life development, alveoli are colonized by embryonic macrophages, which become resident alveolar macrophages (ResAM) and self-sustain by local division. Genetic and epigenetic signatures and, to some extent, the functions of ResAM are dictated by the lung microenvironment, which uses cytokines, ligand-receptor interactions, a...
Article
S. aureus bone infections remain a therapeutic challenge, leading to long and expensive hospitalizations. Systemic antibiotic treatments are inconsistently effective due to insufficient penetration into the infectious site. In an osteomyelitis model, the single local administration of nanoparticle-encapsulated daptomycin allows sterilization of the...
Article
Full-text available
Staphylococcus aureus is the most frequent aetiology of bone and joint infections (BJI) and can cause relapsing and chronic infections. One of the main factors involved in the chronicization of staphylococcal BJIs is the internalization of S. aureus into osteoblasts, the bone-forming cells. Previous studies have shown that S. aureus triggers an imp...
Article
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The world is on the verge of a major antibiotic crisis as the emergence of resistant bacteria is increasing, and very few novel molecules have been discovered since the 1960s. In this context, scientists have been exploring alternatives to conventional antibiotics, such as ribosomally synthesized and post-translationally modified peptides (RiPPs)....
Article
NK cells play a key role in both anti-bacterial and anti-tumor immunity. Pseudomonas aeruginosa (PA) infection has already been reported to depress Natural Killer (NK) cell functions. We studied in vitro the effect of PA on NK cell cytotoxic response (CD107a membrane expression) to a lymphoma cell line. Through positive and negative cell sorting an...
Article
Introduction L’immunodépression (ID) post-septique est l’une des principales causes de mortalité secondaire au décours du sepsis, notamment en raison des infections nosocomiales qui lui sont associées. Une altération sévère des lymphocytes CD4⁺ est l’une des caractéristiques de l’ID post-septique. Les lymphocytes T régulateurs (Treg) sont des cellu...
Article
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
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Sepsis causes inflammation-induced immunosuppression with lymphopenia and alterations of CD4+ T-cell functions that renders the host prone to secondary infections. Whether and how regulatory T cells (Treg) are involved in this postseptic immunosuppression is unknown. We observed in vivo that early activation of Treg during Staphylococcus aureus sep...
Article
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Sepsis and trauma cause inflammation and elevated susceptibility to hospital-acquired pneumonia. As phagocytosis by macrophages plays a critical role in the control of bacteria, we investigated the phagocytic activity of macrophages after resolution of inflammation. After resolution of primary pneumonia, murine alveolar macrophages (AMs) exhibited...
Article
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Impeding as well as reducing the burden of antimicrobial resistance in Gram negative pathogens is an urgent public health endeavor. Our current antibiotic armamentarium is dwindling while major resistance determinants (e.g. extended-spectrum β-lactamases (ESBLs)) continue to evolve and disseminate around the world. One approach to attack this probl...
Article
Objectives: In patients with spinal cord injury, spinal cord injury-immune depression syndrome induces pneumonia. We aimed to develop a new spinal cord injury-immune depression syndrome mouse model and to test antiprogrammed cell death 1 therapy. Design: Experimental study. Setting: Research laboratory. Subjects: RjOrl: SWISS and BALB/cJ mice....
Article
Objectives: To evaluate the significant role played by biofilms during prosthetic vascular material infections (PVMIs). Methods: We developed an in vivo mouse model of Staphylococcus aureus PVMI allowing its direct observation by confocal microscopy to describe: (i) the structure of biofilms developed on Dacron® vascular material; (ii) the local...
Article
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Introduction Un stress aigu (sepsis, choc traumatique) entraîne une immunosuppression post-inflammation et augmente le risque de développer une infection respiratoire secondaire. Le but de ce travail était de comparer les propriétés fonctionnelles des MA pendant et après la résolution de l’inflammation dans un modèle murin de pneumonie et d’extrapo...
Article
Introduction Après un premier essor au début du XXe siècle, la phagothérapie a été rapidement éclipsée dans les pays occidentaux par l’avènement des antibiotiques au début des années 1940. Face à la progression de la résistance bactérienne aux antibiotiques et au faible développement de nouvelles molécules, les phages, virus spécifiques des bactéri...
Article
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Pseudomonas aeruginosa is a major threat for immune-compromised patients. Bacterial pneumonia can induce uncontrolled and massive neutrophil recruitment ultimately leading to acute respiratory distress syndrome and epithelium damage. Interleukin-22 plays a central role in the protection of the epithelium. In this study, we aimed to evaluate the rol...
Article
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Pseudomonas aeruginosa (PA) expresses the type III secretion system (T3SS) and effector exoenzymes that interfere with intracellular pathways. Natural killer (NK) cells play a key role in antibacterial immunity and their activation is highly dependent on IL-12 produced by myeloid cells. We studied PA and NK cell interactions and the role of IL-12 u...
Article
Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-characterized clinical C. acnes iso...
Article
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Objectives: Combination antibiotic therapy has been used successfully to treat some patients with bacterial infections. However, although certain combinations may result in beneficial synergistic activity, others may produce antagonistic effects resulting in poor treatment outcomes. Ceftolozane/tazobactam is an antibacterial agent with potent acti...
Article
Lung infections cause prolonged immune alterations and elevated susceptibility to secondary pneumonia. We found that, after resolution of primary viral or bacterial pneumonia, dendritic cells (DC), and macrophages exhibited poor antigen-presentation capacity and secretion of immunogenic cytokines. Development of these “paralyzed” DCs and macrophage...
Article
Introduction Dans l’endocardite infectieuse à Staphylococcus aureus sensible à la méticilline (SASM), il existe actuellement une controverse concernant l’impact pronostique de la concentration minimale inhibitrice (CMI) à la vancomycine avec de nombreuses études cliniques contradictoires. L’objectif était d’évaluer l’impact de la CMI à la vancomyci...
Article
Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by li...
Presentation
The extensive research devoted to understand biofilms resistance/tolerance to antimicrobials has been oriented on mechanisms involving specific genetic or physiological cell properties but also on antibiotic sorption and/or reaction with biofilm components that may lessen the antimicrobial bioavailability, decreasing its efficiency. This latter hyp...
Poster
Dynamic fluorescence imaging combined with innovative and specific fluorescent probes have greatly improved the research concerning biofilms antimicrobial resistance: it gives the possibility to study non-invasively and in situ antimicrobials localization and activity according to the bacterial physiological state. However, this research currently...
Article
Full-text available
Staphylococcus aureus (S. aureus) is one of the most frequent pathogens responsible for biofilm-associated infections (BAI) and the choice of antibiotics to treat these infections remains a challenge for the medical community. In particular, daptomycin has been reported to fail against implant-associated S. aureus infections in clinical practice wh...
Poster
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Immunotherapy is used in cancerology research, and actually the most studied immunomodulatory molecule is the anti-PD1 antibody. In murine models of sepsis, PD1 cell surface receptor, a marker of “T-cell exhaustion”, has been shown to play a major role in subsequent morbidity. Therapeutic strategies using anti-PD1 antibodies stimulate immune system...
Article
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Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII). Those infections, usually hematogenous, mostly originate from the urinary tract. We investigated the strategies developed by E. coli in this context to evade host innate immune responses, i.e. complement and polymorphonuclear neutrophils (PMN). Twenty...
Article
This study aimed to compare the susceptibility to carbapenems (imipenem, meropenem and doripenem) of clinical strains of Pseudomonas aeruginosa. It also studied whether susceptibility to imipenem or meropenem could predict, reliably, susceptibility to doripenem. Pseudomonal strains were collected from respiratory specimens, half of them from cystic...
Article
Staphylococcus aureus bone and joint infection (BJI) is associated with significant rates of chronicity and relapse. In this study, we investigated how S. aureus is able to adapt to the human environment by comparing isolates from single patients with persisting or relapsing BJIs that were recovered during the initial and recurrent BJI episodes. In...
Article
Objective: Prosthetic vascular graft infection (PVGI) is an emerging disease, mostly caused by staphylococci, with limited data regarding efficacy of current antistaphylococcal agents. We aimed to assess the efficacy of different antibiotic regimens. Methods: Six different strains of MSSA and MRSA were used. We compared results of minimal biofil...
Presentation
Background: Staphylococcus aureus is one of the most frequent pathogens responsible for biofilm­associated infections (BAI) and more specifically those presenting a persistent character, like osteomyelitis or endocarditis. Among clinically­used antibiotics, very few enable long­term successful treatments against BAI. There is therefore a major need...
Presentation
Staphylococcus aureus is one of the most frequent pathogens associated with biofilm-related infections. Among clinically-used antibiotics for the treatment of S. aureus infections, very few enable long-term successful treatments against biofilms. There is therefore a major need to gain a better understanding of these biological structures tolerance...
Poster
Staphylococcus aureus is one of the most frequent pathogens associated with biofilm-related infections. Among clinically-used antibiotics for the treatment of S. aureus infections, very few enable long-term successful treatments against biofilms. There is therefore a major need to gain a better understanding of these biological structures tolerance...
Article
Full-text available
Crohn's disease and ulcerative colitis, the two major forms of inflammatory bowel diseases (IBDs), are characterized by high levels of IL-22 production. Rodent studies revealed that this cytokine is protective during colitis but whether this is true in IBDs is unclear. We show here that levels of the soluble inhibitor of IL-22, interleukin 22-bindi...
Article
Full-text available
Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII), but little is known about the pathogenicity of this species in such infections that are increasing due to the ageing of the population. We report how this pathogen interacts with human osteoblastic MG-63 cells in vitro, by comparing twenty OII E. coli...
Presentation
Full-text available
30% of trauma patients hospitalized intensive care units develop nosocomial infections (Staphylococcus aureus-induced pneumonia), which significantly increase mortality. In cancerology as well as in sepsis or trauma, PD1 cell surface receptor, a marker or “T-cell exhaustion”, has been shown to play a major role. Therapeutic strategies using anti-PD...
Article
Full-text available
Vancomycin is the usual antibiotic treatment in coagulase-negative staphylococcus sepsis in premature infants but causes renal toxicity. As linezolid is effective in Gram-positive cocci infection, and devoid of renal side-effects, it has been used in Nantes neonatal intensive care units and linezolid plasma concentrations were monitored. The aims o...
Article
Full-text available
Objective: Trauma induces a state of immunosuppression, which is responsible for the development of nosocomial infections. Hydrocortisone reduces the rate of pneumonia in patients with trauma. Because alterations of dendritic cells and natural killer cells play a central role in trauma-induced immunosuppression, we investigated whether hydrocortis...
Presentation
Background. Daptomycin, vancomycin and their combination with rifampicin exhibits activity against circulating infections but seem to be less efficient on biofilms. Our work focused on the study of the diffusion, bioavailability and activity of these antibiotics through methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus...
Conference Paper
Full-text available
To assess the ability of the Pseudomonas aeruginosa Type-III Secretion System (T3SS) to alter the protective interleukin-22 (IL22) response in an acute pneumonia mouse model.
Poster
Background. Daptomycin, vancomycin and their combination with rifampicin exhibits activity against circulating infections but seem to be less efficient on biofilms. Our work focused on the study of the diffusion, bioavailability and activity of these antibiotics through methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus...
Article
Full-text available
The aim of this study was to compare ceftolozane with ceftazidime, piperacillin/tazobactam (TZP) and imipenem in an experimental rabbit model of Pseudomonas aeruginosa pneumonia. Efficacy was assessed following 2 days of treatment by total colony counts in different tissues (lung, spleen and blood culture). Mean ± standard deviation pulmonary bacte...
Article
Full-text available
Microbial biofilm contributes to chronic infection and is involved in the pathogenesis of prosthetic joint infections. Biofilms are structurally complex and should be considered a dynamic system able to protect the bacteria from host defence mechanisms and from antibacterial agents. Despite the use of antibiotics recognized as effective against acu...
Poster
Full-text available
Une lésion médullaire peut provoquer une immunosuppression (IS) qui sensibilise l’individu aux infections opportunistes. Les infections pulmonaires retrouvées chez des patients ayant un traumatisme sévère sont souvent dues à Staphylococcus aureus (S. aureus). Les mécanismes amenant à une IS sont encore mal compris. Les cellules Natural killer (NK)...
Article
Full-text available
Pseudomonas aeruginosa infection is a clinically relevant infection involved in pneumonia in ICUs. Understanding the type of immune response initiated by the host during pneumonia would help defining new strategies to interfere with the bacteria pathogenicity. In this setting, the role of natural killer cells remains controversial. We assessed the...
Article
Full-text available
Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this property for use in an in vivo pneumonia mouse mode...
Article
Full-text available
Background: Linezolid is considered as a therapeutic alternative to the use of glycopeptides for the treatment of pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA). Clinical studies reported a potent survival advantage conferred by the oxazolidinone and called into question the use of glycopeptides as first-line therapy. Meth...
Conference Paper
Full-text available
Background: Neutrophils are crucial in the defense against bacteria infection. Type-III secretion system (T3SS) is one of the major virulence factor of Pseudomonas aeruginosa (PA). It includes several exoenzymes (Exo) named S, T, Y and U. Exo can interfere with host cellular mechanisms and induce cell apoptosis once injected into targeted cells. Th...
Article
Full-text available
Infective endocarditis is a worrisome disease, with no significant decrease of the incidence and mortality in the last decade. Despite a changing epidemiology over the last few years, S. aureus remains the leading causative organism in Canada, the U.S., and Europe. Experimental animal models of endocarditis have been extensively used to evaluate th...
Article
Full-text available
Haemorrhage-induced immunosuppression has been linked to nosocomial infections. We assessed the impact of MonoPhosphoryl Lipid A (MPLA), a TIR-domain-containing adaptor protein inducing Interferon-biased Toll Like Receptor 4 agonist currently used as a vaccine adjuvant in humans, on post-haemorrhage susceptibility to infection.We used a mice model...