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Cécile Angebault

Cécile Angebault
CHU Henri Mondor, Assistance Publique – Hôpitaux de Paris · Parasitology-Mycology Unit in Microbiology Department

PharmD, PhD

About

59
Publications
4,928
Reads
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1,478
Citations
Additional affiliations
May 2016 - present
Assistance Publique – Hôpitaux de Paris
Position
  • Praticien Hospitalier Universitaire, CHU Henri Mondor
November 2015 - present
Université Paris-Est Créteil
Position
  • Praticien Hospitalier Universitaire
November 2011 - October 2015
Assistance Publique – Hôpitaux de Paris
Position
  • Assistant Hospitalo-Universitaire, Hôpital Necker-Enfants Malades

Publications

Publications (59)
Article
Introduction Fusarium spp. est un agent fongique phytopathogène et émergent en pathologie humaine chez les patients sévèrement immunodéprimés et grands brûlés. Les fusarioses invasives restent associées à une importante morbi-mortalité en lien avec un diagnostic difficile en l’absence de culture positive. L’objectif de notre étude est d’évaluer la...
Article
Full-text available
Scedosporium species are opportunistic pathogens causing various infections, including disseminated infections in severely immunocompromised patients. Preventive measures aiming to reduce the risk of exposure to these fungi require a better knowledge on their ecology and on the sources of contamination of the patients. In this context, 99 soil samp...
Article
Full-text available
Objectives Aspergillus and Mycobacterium are opportunistic pathogens that can cause severe pulmonary diseases. To date, the clinical significance of their concomitant isolation and potential interactions in the lung remains poorly understood. The aim of this study was to assess the prevalence of their concomitant isolation from respiratory samples,...
Article
Full-text available
Among 419 consecutive allogeneic hematopoietic cell transplant recipients, we observed 17 (4.0%) cases of toxoplasmosis at a median time of day 45 (range, 6 to 322) after transplant. Seven of these 17 cases occurred before day 30 after transplant. Because of the lack of PCR screening and trimethoprim-sulfamethoxazole prophylaxis before engraftment,...
Article
Full-text available
Background The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for bacterial microbiota analysis. Here, we investigated the impact of storage and DNA extraction on b...
Data
Comparison of DNA extracted from fecal samples stored in two different conditions using two extraction protocols. Three fecal samples from healthy individuals stored at -80°C after dilution in RNAlater® or without additive, were submitted to 2 extraction protocols: the IHMS Protocol Q and the PowerSoil® MoBio kit. (TIF)
Data
Boxplot comparison, at individual level, of log2-abundance of bacterial (A) and fungal (B) taxa according to storage condition. Bacterial diversity was assessed at genus level using 16S rRNA gene ultra-deep sequencing (454 technology) and fungal diversity at genus or section level using ITS1 ultra-deep sequencing. Boxplot of log2-abundance of taxa...
Data
Boxplot comparison, at individual level, of log2-abundance of bacterial (A) and fungal (B) taxa according to extraction protocol. Bacterial diversity was assessed at genus level using 16S rRNA gene ultra-deep sequencing (454 technology) and fungal diversity at genus or section level using ITS1 ultra-deep sequencing. Boxplot of log2-abundance of tax...
Data
Abundance Fold Change of bacterial (A) and fungal (B) taxa significantly different according to storage condition at general level. (DOCX)
Data
Rarefaction curves of bacterial (A) and fungal (B) diversity assessed from fecal samples using 16S or ITS1 ultra-deep sequencing (454 technology). Total DNA was extracted from fecal samples of 3 healthy individuals (i1, i2 and i3) using two storage conditions (within two-hours freezing or RNAlater® dilution before freezing) and two extraction proto...
Data
Alpha diversity measurements for bacterial (A) and fungal (B) metagenomic analyzes of 3 fecal samples using 2 storage and 2 extraction conditions. (DOCX)
Data
PCoA plots of bacterial (A) and fungal (B) between-sample dissimilarities according to storage or extraction condition. PCoA plots, computed using bray distance, of fecal samples of 3 healthy individuals (i1 [blue], i2 [green] and i3 [orange]) processed using two storage conditions (within two-hours freezing [triangle] or RNAlater® dilution before...
Data
Results of ultra-deep sequencing (reads, OTUs and assignment) for 16S and ITS1 targets (12 samples). (DOCX)
Data
Abundance Fold Change of bacterial (A) and fungal (B) taxa significantly different according to storage condition at individual level. (DOCX)
Data
Abundance Fold Change of bacterial (A) and fungal (B) taxa significantly different according to extraction protocol at general level. (DOCX)
Data
Abundance Fold Change of bacterial (A) and fungal (B) taxa significantly different according to extraction protocol at individual level. (DOCX)
Article
Full-text available
Given the complexity of the airway microbiota in the respiratory tract of cystic fibrosis (CF) patients, it seems crucial to compile the most exhaustive and exact list of the microbial communities inhabiting CF airways. The aim of the present study was to compare the bacterial and fungal diversity of sputa from adult CF patients during non-exacerba...
Article
Imipenem is active against extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) but favours the intestinal emergence of resistance. The effects of imipenem on intestinal microbiota have been studied using culture-based techniques. In this study, the effects were investigated in patients using culture and metagenomic techniques. Seven...
Article
Full-text available
Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in hematology. Although occasionally reported, the role of interhuman transmission of P. jirovecii in PCP, compared to that of reactivation, remains an unresolved question; the recommendation to isolate PCP patients in the hematology ward are not well evidence-based. Following a...
Article
Background Aspergillus fumigatus is a major opportunistic pathogen causing nosocomial infection. Hospital outbreaks of invasive aspergillosis have been associated with demolition and building construction. This study was designed to examine the impact of meteorologic factors and different periods of work on outdoor fungal airborne concentrations....
Article
Full-text available
Background. Early diagnosis and treatment are crucial in invasive fungal diseases (IFD). Serum (1-3)-β-d-glucan (BG) is believed to be an early IFD marker, but its diagnostic performance has been ambiguous, with insufficient data regarding sensitivity at the time of IFD diagnosis (TOD) and according to outcome. Whether its clinical utility is equiv...
Article
Full-text available
Epidemiological and individual risk factors for colonisation by Enterobacteria producing extended spectrum beta-lactamases (E-ESBL) have been studied extensively, but whether it is associated with significant changes in the composition of the rest of the microbiota is still unknown. To answer this question we assessed in an isolated Amerindian Guia...
Article
Invasive aspergillosis (IA) is a major cause of morbidity and mortality in immunocompromised adults and children, the number of which has been continuously increasing in the last decades. The purpose of our review was to provide epidemiological, clinical, and biological data and antifungal treatment options in the pediatric population. Several biol...
Article
Delay of active antimicrobial therapy in haematological patients with Gram negative bacilli bacteraemia during profound neutropenia exposes them to increased morbidity and mortality. The digestive tract is the main source of enterobacteria causing bacteraemia in these patients. We thus evaluated the usefulness of broad-spectrum beta-lactam - resist...
Presentation
Objectives: The incidence of invasive fungal diseases (IFD) is currently increasing as a consequence of the growing population of immunocompromised patients. A key to IFD prognosis relies on early diagnosis, allowing early initiation of antifungal therapy. Over past years, the detection of the fungal (1-3)-β-D-glucan (BG) antigen has been increasin...
Article
Le mycobiote se réfère à la composante fongique du microbiote vivant au sein d’un environnement donné. Le mycobiote humain fait donc partie du microbiote humain, au même titre que les bactéries. Malgré son importance, il reste peu étudié. De nouvelles approches indépendantes de la culture, comme les méthodes de séquençage à haut débit, développées...
Article
The mycobiota refers to the fungal component of the overall microbiota inhabiting a specific ecosystem. The human mycobiota is thus part of the human microbiota along with bacteria, archeobacteria, protozoa and viruses. Despite its importance, it has remained understudied. However, with to the development of new culture-independent approaches over...
Article
Bacteraemia was reported to be associated with false-positive 1→3-β-D-glucan (BG) assay results. We thus prospectively assessed the reactivity of the BG (Fungitell) in samples of 21 ADULTS WITH BACTERAEMIA: . BG was negative in all and is s therefore an unlikely cause of false positive BG in adults. © The Author 2014. Published by Oxford University...
Article
Full-text available
Solid organ transplant recipients are at risk for invasive fungal diseases, and are also exposed to healthcare-associated mucormycosis. Mainly causing localized cutaneous mucormycosis, Mucor irregularis infection is reported for the first time in a kidney-transplant recipient. A healthcare-associated origin was highly suspected in this case. We per...
Article
An increasing number of infections due to Pseudallescheria/Scedosporium species has been reported during the past decades, both in immunocompromised and immunocompetent patients. Additionally, these fungi are now recognized worldwide as common agents of fungal colonization of the airways in cystic fibrosis patients, which represents a risk factor f...
Article
Full-text available
Carriage of extended-spectrum beta-lactamase-producing enterobacteria (ESBL-E) has increased in community settings. Little is known about their long-term evolution. French Guiana Amerindians living in a very remote village, already sampled in 2001 and 2006 for ESBL-E fecal carriage, were screened again in October 2010. Sociodemographic data and ant...
Article
Full-text available
Extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli (ESBL E. coli) strains are of major concern because few antibiotics remain active against these bacteria. We investigated the association between the fecal relative abundance (RA) of ESBL-producing E. coli (ESBL-RA) and the occurrence of ESBL E. coli urinary tract infections (UTIs)....
Article
Full-text available
In industrialized countries Candida albicans is considered the predominant commensal yeast of the human intestine, with approximately 40% prevalence in healthy adults. We discovered a highly original colonization pattern that challenges this current perception by studying in a 4- year interval a cohort of 151 Amerindians living in a remote communit...
Article
Full-text available
Azole resistance has appeared recently in Aspergillus fumigatus and increased dangerously in the last decade. The main resistance mechanism is a point mutation of CYP51A, the gene encoding 14α-sterol demethylase, the target enzyme of azole antifungal drugs. This mutation can induce resistance to itraconazole alone or multi-azole resistance. CYP51A...
Article
MALDI-TOF mass spectrometry (MALDI-TOF MS) is a new powerful tool increasingly used in microbiological laboratories over the past years to identify rapidly and reliably bacterial and yeast microorganisms isolated from clinical samples. In the present review, we will describe the principle and the main features of MALDI-TOF MS use for yeast identifi...
Article
Full-text available
Intestinal flora contains a reservoir of Gram-negative bacilli (GNB) resistant to cephalosporins, which are potentially pathogenic for intensive care unit (ICU) patients; this has led to increasing use of carbapenems. The emergence of carbapenem resistance is a major concern for ICUs. Therefore, in this study, we aimed to assess the intestinal carr...
Article
Antibiotics are essential agents that have greatly reduced human mortality due to infectious diseases. Their use, and sometimes overuse, have increased over the past several decades in humans, veterinary medicine and agriculture. However, the emergence of resistant pathogens is becoming an increasing problem that could result in the re-emergence of...
Article
Full-text available
Nasal carriage of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) is highly prevalent in community subjects, but its dynamic has been little investigated. Nasal swabbing was performed in 2006 and 2008 in 154 Amerindians living isolated in French Guiana. MR-CoNS strains were identified and characterized by non-β-lactam susceptibilit...
Article
Full-text available
From the time of CTX-M emergence, extended-spectrum β-lactamase-producing enterobacteria (ESBL-E) have spread worldwide in community settings as well as in hospitals, particularly in developing countries. Although their dissemination appears linked to Escherichia coli intestinal carriage, precise paths of this dynamic are largely unknown. Children...
Article
Full-text available
Melioidosis is a disease caused by Burkholderia pseudomallei and considered endemic in South-East Asia but remains poorly documented in Cambodia. We report the first series of hospitalized pulmonary melioidosis cases identified in Cambodia describing clinical characteristics and outcomes. We characterized cases of acute lower respiratory infections...
Article
Full-text available
The three major soil-transmitted helminths (STH) Ascaris lumbricoides, Trichuris trichiura and Necator americanus/Ancylostoma duodenale are among the most widespread parasites worldwide. Despite the global expansion of preventive anthelmintic treatment, standard operating procedures to monitor anthelmintic drug efficacy are lacking. The objective o...
Article
Full-text available
Staphylococcus aureus nasal carriage is influenced by multifactorial interactions which are difficult to study in open populations. Therefore, we concomitantly assessed the epidemiological, microbiological, and human-genetic carriage-related factors in a nearly closed population. In 2006 and 2008, we collected nasal S. aureus strains, human DNA, an...
Article
Full-text available
Intestinal carriage is a key factor in extended-spectrum beta-lactamase (ESBL) infection epidemiology but is difficult to study in open communities. To overcome this problem, we studied a highly stable group of Amerindians for whom we reported an ESBL carriage prevalence of 3.2% in 2001. In 2006, ESBL carriage was assessed among 163 healthy volunte...

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